| 出品人: | Addgene |
|---|---|
| 载体名称: | pBABE-neo-hTERT |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 8709 bp |
| 5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
| 3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素和卡那霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | DH5α、HB101等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
逆病毒载体pBABE-neo-hTERT表达telomerase reverse transcriptase，用来建永生细胞系。 推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | #1774 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
pBABE-neo-hTERT载体质粒基本信息
pBABE-neo-hTERT质粒图谱载体图谱和pBABE-neo-hTERT载体序列质粒序列多克隆位点信息
pBABE-neo-hTERT质粒载体简介
pBABE-neo-hTERT质粒序列载体序列
LOCUS pBABE-neo-hTERT Sequencing Result 8709 bp DNA SYN
DEFINITION pBABE-neo-hTERT Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8709
/organism="pBABE-neo-hTERT Sequencing Result"
/mol_type="other DNA"
misc_feature 10..477
/label="5_LTR2"
misc_feature 16..477
/label="3MoMuLV_LTR"
misc_feature 547..1356
/label="psi_plus_pack"
misc_feature 943..1350
/label="gag"
misc_feature 1286..1308
/label="MSCV_primer"
misc_feature 1286..1308
/label="pLXSN_5_primer"
misc_feature 1324..1340
/label="pBABE_5_primer"
CDS complement(1337..2050)
/label="ORF frame 3"
/translation="MVPGPFASQTPSGSTSVWRGPGLSGSAELVQRRPAHLVGAAGSH
QHKERAACQQVNQHVVAHAAQQQPPRPAPPQCVGHRVGQVAAHAGGEGLGGAPAGPVQ
QREAEGQHVLRAALAQPLQHSGHQLLQAGHLAEGGGGGGRPCVPGHAHQALGHQRAES
RRVPALHQPPALGPQAPHERGQRQHLAVVAAQQGAHGSAAGSARHRGGGRGQGFPRAQ
QDEFLRTTTLGSGRRLEKE*"
CDS 1436..4834
/label="ORF frame 2"
/translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDP
AAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFA
LLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFV
LVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPA
PGARRRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPA
RPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYS
SGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPL
FLELLGNHAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQ
LLRQHSSPWQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQEL
TWKMSVRGCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTET
TFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFI
PKPDGLRPIVNMDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLG
LDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYC
VRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSL
NEASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAG
IRRDGLLLRLVDDFLLVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEAL
GGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRR
KLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPT
FFLRVISDTASLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVT
YVPLLGSLRTAQTQLSRKLPGTTLTALEAAANPALPSDFKTILD*"
CDS complement(2471..3691)
/label="ORF frame 3"
/translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
EPPSASQAGPQRAE*"
misc_feature complement(4846..4866)
/label="pBABE_3_primer"
/translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
EPPSASQAGPQRAE*"
misc_feature complement(4852..5067)
/label="SV40_enhancer"
/translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
EPPSASQAGPQRAE*"
promoter 4864..5132
/label="SV40_promoter"
/translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
EPPSASQAGPQRAE*"
rep_origin 5031..5108
/label="SV40_origin"
/translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
EPPSASQAGPQRAE*"
misc_feature 5093..5112
/label="SV40pro_F_primer"
/translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
EPPSASQAGPQRAE*"
promoter 5399..5448
/label="NEOKAN_promoter"
/translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
EPPSASQAGPQRAE*"
CDS 5537..6331
/label="ORF frame 2"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 5540..6328
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(5846..6352)
/label="ORF frame 3"
/translation="MRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAVSPFAAKLFS
NITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVGHARLEPGE
QFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPHCISHDGY
FLGRSKVR*"
gene complement(7719..8579)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAVSPFAAKLFS
NITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVGHARLEPGE
QFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPHCISHDGY
FLGRSKVR*"
CDS complement(7719..8579)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(8621..8649)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccGGCTCAGG
61 GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC
121 CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT
181 TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT
241 ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC
301 TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC
361 CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG
421 TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCATTTGG
481 GGGCTCGTCC GGGATCGGGA GACCCCTGCC CAGGGACCAC CGACCCACCA CCGGGAGGCA
541 AGCTGGCCAG CAACTTATCT GTGTCTGTCC GATTGTCTAG TGTCTATGAC TGATTTTATG
601 CGCCTGCGTC GGTACTAGTT AGCTAACTAG CTCTGTATCT GGCGGACCCG TGGTGGAACT
661 GACGAGTTCT GAACACCCGG CCGCAACCCT GGGAGACGTC CCAGGGACTT TGGGGGCCGT
721 TTTTGTGGCC CGACCTGAGG AAGGGAGTCG ATGTGGAATC CGACCCCGTC AGGATATGTG
781 GTTCTGGTAG GAGACGAGAA CCTAAAACAG TTCCCGCCTC CGTCTGAATT TTTGCTTTCG
841 GTTTGGAACC GAAGCCGCGC GTCTTGTCTG CTGCAGCGCT GCAGCATCGT TCTGTGTTGT
901 CTCTGTCTGA CTGTGTTTCT GTATTTGTCT GAAAATTAGG GCCAGACTGT TACCACTCCC
961 TTAAGTTTGA CCTTAGGTCA CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA
1021 GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC
1081 GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC
1141 TTTTCACCTG GCCCGCATGG ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC
1201 TTGGCTTTTG ACCCCCCTCC CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT
1261 CTTCCTCCAT CCGCCCCGTC TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC
1321 TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCGGCC GGATCCCAGT GTGGTGGTAC
1381 GTAGGAATTC GTCCTGCTGC GCACGTGGGA AGCCCTGGCC CCGGCCACCC CCGCGATGCC
1441 GCGCGCTCCC CGCTGCCGAG CCGTGCGCTC CCTGCTGCGC AGCCACTACC GCGAGGTGCT
1501 GCCGCTGGCC ACGTTCGTGC GGCGCCTGGG GCCCCAGGGC TGGCGGCTGG TGCAGCGCGG
1561 GGACCCGGCG GCTTTCCGCG CGCTGGTGGC CCAGTGCCTG GTGTGCGTGC CCTGGGACGC
1621 ACGGCCGCCC CCCGCCGCCC CCTCCTTCCG CCAGGTGTCC TGCCTGAAGG AGCTGGTGGC
1681 CCGAGTGCTG CAGAGGCTGT GCGAGCGCGG CGCGAAGAAC GTGCTGGCCT TCGGCTTCGC
1741 GCTGCTGGAC GGGGCCCGCG GGGGCCCCCC CGAGGCCTTC ACCACCAGCG TGCGCAGCTA
1801 CCTGCCCAAC ACGGTGACCG ACGCACTGCG GGGGAGCGGG GCGTGGGGGC TGCTGCTGCG
1861 CCGCGTGGGC GACGACGTGC TGGTTCACCT GCTGGCACGC TGCGCGCTCT TTGTGCTGGT
1921 GGCTCCCAGC TGCGCCTACC AGGTGTGCGG GCCGCCGCTG TACCAGCTCG GCGCTGCCAC
1981 TCAGGCCCGG CCCCCGCCAC ACGCTAGTGG ACCCCGAAGG CGTCTGGGAT GCGAACGGGC
2041 CTGGAACCAT AGCGTCAGGG AGGCCGGGGT CCCCCTGGGC CTGCCAGCCC CGGGTGCGAG
2101 GAGGCGCGGG GGCAGTGCCA GCCGAAGTCT GCCGTTGCCC AAGAGGCCCA GGCGTGGCGC
2161 TGCCCCTGAG CCGGAGCGGA CGCCCGTTGG GCAGGGGTCC TGGGCCCACC CGGGCAGGAC
2221 GCGTGGACCG AGTGACCGTG GTTTCTGTGT GGTGTCACCT GCCAGACCCG CCGAAGAAGC
2281 CACCTCTTTG GAGGGTGCGC TCTCTGGCAC GCGCCACTCC CACCCATCCG TGGGCCGCCA
2341 GCACCACGCG GGCCCCCCAT CCACATCGCG GCCACCACGT CCCTGGGACA CGCCTTGTCC
2401 CCCGGTGTAC GCCGAGACCA AGCACTTCCT CTACTCCTCA GGCGACAAGG AGCAGCTGCG
2461 GCCCTCCTTC CTACTCAGCT CTCTGAGGCC CAGCCTGACT GGCGCTCGGA GGCTCGTGGA
2521 GACCATCTTT CTGGGTTCCA GGCCCTGGAT GCCAGGGACT CCCCGCAGGT TGCCCCGCCT
2581 GCCCCAGCGC TACTGGCAAA TGCGGCCCCT GTTTCTGGAG CTGCTTGGGA ACCACGCGCA
2641 GTGCCCCTAC GGGGTGCTCC TCAAGACGCA CTGCCCGCTG CGAGCTGCGG TCACCCCAGC
2701 AGCCGGTGTC TGTGCCCGGG AGAAGCCCCA GGGCTCTGTG GCGGCCCCCG AGGAGGAGGA
2761 CACAGACCCC CGTCGCCTGG TGCAGCTGCT CCGCCAGCAC AGCAGCCCCT GGCAGGTGTA
2821 CGGCTTCGTG CGGGCCTGCC TGCGCCGGCT GGTGCCCCCA GGCCTCTGGG GCTCCAGGCA
2881 CAACGAACGC CGCTTCCTCA GGAACACCAA GAAGTTCATC TCCCTGGGGA AGCATGCCAA
2941 GCTCTCGCTG CAGGAGCTGA CGTGGAAGAT GAGCGTGCGG GGCTGCGCTT GGCTGCGCAG
3001 GAGCCCAGGG GTTGGCTGTG TTCCGGCCGC AGAGCACCGT CTGCGTGAGG AGATCCTGGC
3061 CAAGTTCCTG CACTGGCTGA TGAGTGTGTA CGTCGTCGAG CTGCTCAGGT CTTTCTTTTA
3121 TGTCACGGAG ACCACGTTTC AAAAGAACAG GCTCTTTTTC TACCGGAAGA GTGTCTGGAG
3181 CAAGTTGCAA AGCATTGGAA TCAGACAGCA CTTGAAGAGG GTGCAGCTGC GGGAGCTGTC
3241 GGAAGCAGAG GTCAGGCAGC ATCGGGAAGC CAGGCCCGCC CTGCTGACGT CCAGACTCCG
3301 CTTCATCCCC AAGCCTGACG GGCTGCGGCC GATTGTGAAC ATGGACTACG TCGTGGGAGC
3361 CAGAACGTTC CGCAGAGAAA AGAGGGCCGA GCGTCTCACC TCGAGGGTGA AGGCACTGTT
3421 CAGCGTGCTC AACTACGAGC GGGCGCGGCG CCCCGGCCTC CTGGGCGCCT CTGTGCTGGG
3481 CCTGGACGAT ATCCACAGGG CCTGGCGCAC CTTCGTGCTG CGTGTGCGGG CCCAGGACCC
3541 GCCGCCTGAG CTGTACTTTG TCAAGGTGGA TGTGACGGGC GCGTACGACA CCATCCCCCA
3601 GGACAGGCTC ACGGAGGTCA TCGCCAGCAT CATCAAACCC CAGAACACGT ACTGCGTGCG
3661 TCGGTATGCC GTGGTCCAGA AGGCCGCCCA TGGGCACGTC CGCAAGGCCT TCAAGAGCCA
3721 CGTCTCTACC TTGACAGACC TCCAGCCGTA CATGCGACAG TTCGTGGCTC ACCTGCAGGA
3781 GACCAGCCCG CTGAGGGATG CCGTCGTCAT CGAGCAGAGC TCCTCCCTGA ATGAGGCCAG
3841 CAGTGGCCTC TTCGACGTCT TCCTACGCTT CATGTGCCAC CACGCCGTGC GCATCAGGGG
3901 CAAGTCCTAC GTCCAGTGCC AGGGGATCCC GCAGGGCTCC ATCCTCTCCA CGCTGCTCTG
3961 CAGCCTGTGC TACGGCGACA TGGAGAACAA GCTGTTTGCG GGGATTCGGC GGGACGGGCT
4021 GCTCCTGCGT TTGGTGGATG ATTTCTTGTT GGTGACACCT CACCTCACCC ACGCGAAAAC
4081 CTTCCTCAGG ACCCTGGTCC GAGGTGTCCC TGAGTATGGC TGCGTGGTGA ACTTGCGGAA
4141 GACAGTGGTG AACTTCCCTG TAGAAGACGA GGCCCTGGGT GGCACGGCTT TTGTTCAGAT
4201 GCCGGCCCAC GGCCTATTCC CCTGGTGCGG CCTGCTGCTG GATACCCGGA CCCTGGAGGT
4261 GCAGAGCGAC TACTCCAGCT ATGCCCGGAC CTCCATCAGA GCCAGTCTCA CCTTCAACCG
4321 CGGCTTCAAG GCTGGGAGGA ACATGCGTCG CAAACTCTTT GGGGTCTTGC GGCTGAAGTG
4381 TCACAGCCTG TTTCTGGATT TGCAGGTGAA CAGCCTCCAG ACGGTGTGCA CCAACATCTA
4441 CAAGATCCTC CTGCTGCAGG CGTACAGGTT TCACGCATGT GTGCTGCAGC TCCCATTTCA
4501 TCAGCAAGTT TGGAAGAACC CCACATTTTT CCTGCGCGTC ATCTCTGACA CGGCCTCCCT
4561 CTGCTACTCC ATCCTGAAAG CCAAGAACGC AGGGATGTCG CTGGGGGCCA AGGGCGCCGC
4621 CGGCCCTCTG CCCTCCGAGG CCGTGCAGTG GCTGTGCCAC CAAGCATTCC TGCTCAAGCT
4681 GACTCGACAC CGTGTCACCT ACGTGCCACT CCTGGGGTCA CTCAGGACAG CCCAGACGCA
4741 GCTGAGTCGG AAGCTCCCGG GGACGACGCT GACTGCCCTG GAGGCCGCAG CCAACCCGGC
4801 ACTGCCCTCA GACTTCAAGA CCATCCTGGA CTGAGTCGAC CCTGTGGAAT GTGTGTCAGT
4861 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA
4921 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA
4981 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC
5041 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG
5101 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG
5161 GAGGCCTAGG CTTTTGCAAA AAGCTTCACG CTGCCGCAAG CACTCAGGGC GCAAGGGCTG
5221 CTAAAGGAAG CGGAACACGT AGAAAGCCAG TCCGCAGAAA CGGTGCTGAC CCCGGATGAA
5281 TGTCAGCTAC TGGGCTATCT GGACAAGGGA AAACGCAAGC GCAAAGAGAA AGCAGGTAGC
5341 TTGCAGTGGG CTTACATGGC GATAGCTAGA CTGGGCGGTT TTATGGACAG CAAGCGAACC
5401 GGAATTGCCA GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT
5461 GGCTTTCTTG CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGATCTGATC AAGAGACAGG
5521 ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
5581 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
5641 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
5701 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
5761 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
5821 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
5881 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
5941 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
6001 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
6061 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA
6121 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC
6181 GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA
6241 ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC
6301 CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC ATCGATGCAT
6361 CGATGCGGCG GGTGTGGTGG TTACGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC
6421 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG
6481 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC
6541 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG
6601 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG
6661 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA
6721 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG
6781 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA
6841 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT
6901 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA
6961 GCAACGCATC GATAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG
7021 ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA
7081 ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT
7141 ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG
7201 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
7261 CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC TCAGCAGTTT CTAGAGAACC
7321 ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA
7381 CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA
7441 GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG
7501 TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG
7561 GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCACATGCA GCATGTATCA
7621 AAATTAATTT GGTTTTTTTT CTTAAGTATT TACATTAAAT GGCCATAGTG CGGCCGCAAA
7681 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
7741 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
7801 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
7861 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
7921 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
7981 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
8041 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
8101 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
8161 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
8221 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
8281 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
8341 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
8401 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
8461 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
8521 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
8581 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
8641 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
8701 GTGCCACCT
//
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pBABE-neo-hTERT质粒载体应用举例
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