| 出品人: | Bob Weinberg |
|---|---|
| 载体名称: | pBABE-neo-SV40LT |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 7464 bp |
| 5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
| 3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | DH5α、HB101等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
逆病毒载体pBABE-neo-SV40LT表达Large T Antigen，用来建永生细胞系。 推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | #1780 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 7464 bp ds-DNA circular SYN 07-AUG-2016
DEFINITION synthetic circular DNA.
KEYWORDS pBABE-neo SV40LT
SOURCE synthetic DNA construct
REFERENCE 1 (bases 1 to 7464)
AUTHORS Biofeng Lab.
FEATURES Location/Qualifiers
source 1..7464
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 10..477
/note="5'LTR2"
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 16..477
/note="3MoMuLV LTR"
misc_feature 540..739
/note="MMLV Psi"
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
misc_feature 547..1356
/note="psi plus pack"
CDS 940..1356
/note="gag"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
CDS 1394..3520
/codon_start=1
/note="ORF frame 1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
misc_feature 1652..3520
/note="SV40LT"
/note="Used to create immortalized cell lines"
misc_feature 1769..1789
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
misc_feature 1775..1789
/note="NLS"
promoter 3606..3935
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
enhancer 3607..3822
/note="SV40 enhancer"
rep_origin 3786..3921
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 4155..4203
/note="Neo/Kan promoter"
CDS 4292..5086
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
LTR 5890..6359
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS complement(6474..7334)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7335..7439)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
61 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
121 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
181 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
241 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
301 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
361 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
421 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
481 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
541 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
601 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
661 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
721 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
781 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
841 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
901 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
961 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
1021 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
1081 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
1141 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
1201 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
1261 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
1321 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccgcg accggtcgac
1381 cagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
1441 tctaggtctt gaaaggagtg cctgggggaa tattcctctg atgagaaagg catatttaaa
1501 aaaatgcaag gagtttcatc ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa
1561 tactctgtac aagaaaatgg aagatggagt aaaatatgct catcaacctg actttggagg
1621 cttctgggat gcaactgaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
1681 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
1741 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
1801 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
1861 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
1921 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
1981 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
2041 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
2101 gactagagat ccattttctg ttattgagga aagtttgcca ggtgggttaa aggagcatga
2161 ttttaatcca gaagaagcag aggaaactaa acaagtgtcc tggaagcttg taacagagta
2221 tgcaatggaa acaaaatgtg atgatgtgtt gttattgctt gggatgtact tggaatttca
2281 gtacagtttt gaaatgtgtt taaaatgtat taaaaaagaa cagcccagcc actataagta
2341 ccatgaaaag cattatgcaa atgctgctat atttgctgac agcaaaaacc aaaaaaccat
2401 atgccaacag gctgttgata ctgttttagc taaaaagcgg gttgatagcc tacaattaac
2461 tagagaacaa atgttaacaa acagatttaa tgatcttttg gataggatgg atataatgtt
2521 tggttctaca ggctctgctg acatagaaga atggatggct ggagttgctt ggctacactg
2581 tttgttgccc aaaatggatt cagtggtgta tgacttttta aaatgcatgg tgtacaacat
2641 tcctaaaaaa agatactggc tgtttaaagg accaattgat agtggtaaaa ctacattagc
2701 agctgctttg cttgaattat gtggggggaa agctttaaat gttaatttgc ccttggacag
2761 gctgaacttt gagctaggag tagctattga ccagttttta gtagtttttg aggatgtaaa
2821 gggcactgga ggggagtcca gagatttgcc ttcaggtcag ggaattaata acctggacaa
2881 tttaagggat tatttggatg gcagtgttaa ggtaaactta gaaaagaaac acctaaataa
2941 aagaactcaa atatttcccc ctggaatagt caccatgaat gagtacagtg tgcctaaaac
3001 actgcaggcc agatttgtaa aacaaataga ttttaggccc aaagattatt taaagcattg
3061 cctggaacgc agtgagtttt tgttagaaaa gagaataatt caaagtggca ttgctttgct
3121 tcttatgtta atttggtaca gacctgtggc tgagtttgct caaagtattc agagcagaat
3181 tgtggagtgg aaagagagat tggacaaaga gtttagtttg tcagtgtatc aaaaaatgaa
3241 gtttaatgtg gctatgggaa ttggagtttt agattggcta agaaacagtg atgatgatga
3301 tgaagacagc caggaaaatg ctgataaaaa tgaagatggt ggggagaaga acatggaaga
3361 ctcagggcat gaaacaggca ttgattcaca gtcccaaggc tcatttcagg cccctcagtc
3421 ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
3481 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
3541 ttaacgggga tcccagtgtg gtggtacgta ggaattcgcc agcacagtgg tcgaccctgt
3601 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3661 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
3721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
3781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
3841 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
3901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tcacgctgcc gcaagcactc
3961 agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg
4021 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
4081 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
4141 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
4201 caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc
4261 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
4321 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
4381 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
4441 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
4501 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
4561 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
4621 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
4681 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
4741 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
4801 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
4861 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
4921 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
4981 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
5041 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
5101 ttcgcatcga tgcatcgatg cggcgggtgt ggtggttacg cgtaatctgc tgcttgcaaa
5161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
5221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
5281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
5341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
5401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
5461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
5521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
5581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
5641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
5701 tatggaaaaa cgccagcaac gcatcgataa aataaaagat tttatttagt ctccagaaaa
5761 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
5821 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
5881 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
5941 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
6001 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
6061 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
6121 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
6181 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
6241 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
6301 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
6361 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
6421 tagtgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
6481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
6541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
6601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
6661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
6721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
6781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
6841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
6901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
6961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
7021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
7081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
7141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
7201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
7261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
7321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
7381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
7441 acatttcccc gaaaagtgcc acct
//
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