| 出品公司: | ATCC |
|---|---|
| 载体名称: | pBad33, pBad 33, pBad-33 |
| 质粒类型: | 大肠杆菌蛋白表达载体 |
| 表达水平: | 高 |
| 启动子: | araC |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5352 bp |
| 5' 测序引物及序列: | pBAD-F: ATGCCATAGCATTTTTATCC |
| 3' 测序引物及序列: | pBAD-R: gatttaatctgtatcagg |
| 载体标签: | / |
| 载体抗性: | 氯霉素 10 ug/ml |
| 备注: | 表达启动子是arabinose Bad启动子。 |
| 产品目录号: | 87402 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 诱导表达 |
| 病毒/非病毒: | 非病毒 |
买家导航
pBAD33载体基本信息
pBAD33载体图谱质粒图谱和多克隆位点信息
pBAD33载体简介
pBAD33载体序列
LOCUS pBAD33 5352 bp DNA circular SYN
DEFINITION pBAD33
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5352
/organism="pBAD33"
/mol_type="other DNA"
misc_feature complement(96..974)
/label="AraC"
CDS complement(96..1025)
/label="ORF frame 2"
misc_feature 1004..1019
/label="araO2"
misc_feature 1161..1172
/label="araO1"
misc_feature 1203..1216
/label="CAP_BS"
misc_feature 1208..1227
/label="pBAD_fwd_primer"
misc_feature 1213..1251
/label="AraI1I2"
promoter 1248..1276
/label="ARA_promoter"
misc_feature complement(1395..1412)
/label="pTrcHis_rev_primer"
misc_feature complement(1395..1412)
/label="pBAD_rev_primer"
terminator 1445..1602
/label="rrnB_terminator"
terminator 1568..1611
/label="rrnB_T1_terminator"
terminator 1743..1770
/label="rrnB_T2_terminator"
promoter 1811..1839
/label="AmpR_promoter"
gene 2008..2292
/label="Ampicillin (576 - 860)"
/gene="Ampicillin (576 - 860)"
rep_origin 2351..2650
/label="f1_origin"
gene complement(3344..4003)
/label="CAT/CamR"
/gene="CAT/CamR"
CDS complement(3344..4003)
/label="ORF frame 3"
rep_origin 4365..5204
/label="p15A_origin"
misc_feature 5249..5268
/label="pBRrevBam_primer"
ORIGIN
1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt
1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1981 ctgaagatca gttggggcaa actattaact ggcgaactac ttactctagc ttcccggcaa
2041 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
2101 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
2161 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
2221 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
2281 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc
2341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
2401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
2461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
2521 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
2581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
2641 gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
2701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
2761 ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata
2821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
2881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
2941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
3001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
3061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
3121 tcctgttacc agtcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
3181 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
3241 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
3301 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
3361 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga
3421 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
3481 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
3541 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
3601 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
3661 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
3721 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
3781 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
3841 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
3901 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
3961 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
4021 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
4081 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
4141 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
4201 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga
4261 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
4321 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
4381 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
4441 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
4501 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
4561 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
4621 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
4681 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
4741 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
4801 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
4861 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
4921 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
4981 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
5041 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
5101 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
5161 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttg ctcatgagcc
5221 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
5281 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt
5341 tgacagctta tc
//
