出品公司: | Addgene |
---|---|
载体名称: | pLKO.1-TRC |
质粒类型: | 慢病毒载体;RNAi载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | AgeI,EcoRI |
启动子: | U6 |
载体大小: | 8901 bp |
5' 测序引物及序列: | LKO.15' :GACTATCATATGCTTACCGT |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素 |
克隆菌株: | Stbl3 |
宿主细胞(系): | 哺乳动物细胞 |
备注: |
This is the recommended vector for cloning and expressing new shRNA sequences. This plasmid is being used by The RNAi Consortium to produce their shRNA library http://www.broad.mit.edu/genome_bio/trc/ The 1.9kb stuffer can be released with AgeI and EcoRI and replaced with your shRNA sequence of choice. |
产品目录号: | #10878 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLKO.1-TRC载体 pLK0.1-TRC质粒基本信息
pLKO.1-TRC质粒图谱 pLK0.1-TRC载体图谱和pLKO.1-TRC载体序列 pLK0.1-TRC质粒序列多克隆位点信息
pLKO.1-TRC质粒 pLK0.1-TRC载体简介
pLKO.1-TRC质粒序列 pLK0.1-TRC载体序列
pLKO.1-TRC是pLKO.1 puro载体在AgeI和EcoRI限制性内切酶位点中间插入有片段情况下的质粒,可以用来替代pLKO.1 puro载体。 LOCUS pLKO.1 - TRC cloning vector 8901 bp DNA circular SYN DEFINITION pLKO.1 - TRC cloning vector ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..8901 /organism="pLKO.1 - TRC cloning vector" /mol_type="other DNA" CDS 289..1128 /label="ORF frame 1" CDS complement(334..1179) /label="ORF frame 1" gene 529..1128 /label="puro (variant)" /gene="puro (variant)" misc_feature 1239..1254 /label="cPPT" misc_feature 1239..1260 /label="U3PPT" misc_feature 1256..1308 /label="delta_U3" misc_feature 1309..1489 /label="HIV-1_5_LTR" misc_feature 1309..1489 /label="truncHIV-1_3_LTR" terminator 1564..1683 /label="SV40_PA_terminator" misc_feature 1652..1671 /label="EBV_rev_primer" rep_origin 1701..1799 /label="SV40_origin" misc_feature 1784..1803 /label="SV40pro_F_primer" promoter complement(1878..1896) /label="T7_promoter" promoter complement(1906..1922) /label="M13_forward20_primer" misc_feature complement(1915..1937) /label="M13_pUC_fwd_primer" misc_feature 1915..2063 /label="lacZ_a" rep_origin 2081..2387 /label="f1_origin" promoter 2580..2608 /label="AmpR_promoter" gene 2650..3510 /label="Ampicillin" /gene="Ampicillin" CDS 2650..3510 /label="ORF frame 1" rep_origin 3665..4284 /label="pBR322_origin" promoter 4593..4622 /label="lac_promoter" misc_feature 4636..4658 /label="M13_pUC_rev_primer" promoter 4657..4675 /label="M13_reverse_primer" promoter 4692..4711 /label="T3_promoter" misc_feature 4965..5145 /label="HIV-1_5_LTR" misc_feature 4965..5145 /label="truncHIV-1_3_LTR" misc_feature 5256..5300 /label="HIV-1_psi_pack" CDS 5688..6545 /label="ORF frame 3" misc_feature 5810..6043 /label="RRE" promoter 6579..6811 /label="hU6_promoter" misc_feature 6741..6760 /label="LKO1_5_primer" misc_feature 8736..8751 /label="cPPT" CDS complement(8817..530) /label="ORF frame 2" ORIGIN 1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac 541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 721 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 901 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 1021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 1081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac 1141 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca 1201 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 1261 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 1321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 1381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 1441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 1501 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 1561 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 1621 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 1681 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 1741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 1801 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 1861 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 1921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 1981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 2041 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 2101 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 2161 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 2221 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 2281 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 2341 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 2401 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 2461 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 2521 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 2581 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 2641 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 2701 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 2761 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 2821 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 2881 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 2941 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 3001 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 3061 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 3121 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 3181 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 3241 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 3301 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 3361 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 3421 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 3481 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 3541 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 3601 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 3661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 3721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 3781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 3841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 3901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 3961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 4021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 4081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 4141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 4201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 4261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 4321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 4381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 4441 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 4501 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 4561 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 4621 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 4681 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt 4741 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct 4801 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg 4861 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc 4921 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag 4981 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 5041 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 5101 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag 5161 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga 5221 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 5281 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 5341 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 5401 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 5461 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 5521 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 5581 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 5641 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 5701 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 5761 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 5821 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg 5881 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 5941 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 6001 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 6061 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 6121 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 6181 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 6241 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 6301 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 6361 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 6421 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 6481 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag 6541 actagcctcg agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt 6601 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa 6661 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt 6721 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt 6781 tcttggcttt atatatcttg tggaaaggac gaaacACCGG TGTGTTGTAA ATGAGCACAC 6841 AAAATACACA TGCTAAAATA TTATATTCTA TGACCTTTAT AAAATCAACC AAAATCTTCT 6901 TTTTAATAAC TTTAGTATCA ATAATTAGAA TTTTTATGTT CCTTTTTGCA AACTTTTAAT 6961 AAAAATGAGC AAAATAAAAA AACGCTAGTT TTAGTAACTC GCGTTGTTTT CTTCACCTTT 7021 AATAATAGCT ACTCCACCAC TTGTTCCTAA GCGGTCAGCT CCTGCTTCAA TCATTTTTTG 7081 AGCATCTTCA AATGTTCTAA CTCCACCAGC TGCTTTAACT AAAGCATTGT CTTTAACAAC 7141 TGACTTCATT AGTTTAACAT CTTCAAATGT TGCACCTGAT TTTGAAAATC CTGTTGATGT 7201 TTTAACAAAT TCTAATCCAG CTTCAACAGC TATTTCACAA GCTTTCATGA TTTCTTCTTT 7261 TGTTAATAAA CAATTTTCCA TAATACATTT AACAACATGT GATCCAGCTG CTTTTTTTAC 7321 AGCTTTCATG TCTTCTAAAA CTAATTCATA ATTTTTGTCT TTTAATGCAC CAATATTTAA 7381 TACCATATCA ATTTCTGTTG CACCATCTTT AATTGCTTCA GAAACTTCGA ATGCTTTTGT 7441 AGCTGTTGTG CATGCACCTA GAGGAAAACC TACAACATTT GTTATTCCTA CATTTGTGCC 7501 TTTTAATAAT TCTTTACAAT AGCTTGTTCA ATATGAATTA ACACAAACTG TTGCAAAATC 7561 AAATTCAATT GCTTCATCAC ATAATTGTTT AATTTCAGCT TTCGTAGCAT CTTGTTTTAA 7621 TAATGTGTGA TCTATATATT TGTTTAGTTT CATTTTTTCT CCTATATATT CATTTTTAAT 7681 TTTAATTCTT TAATAATTTC GTCTACTTTA ACTTTAGCGT TTTGAACAGA TTCACCAACA 7741 CCTATAAAAT AAATTTTTAG TTTAGGTTCA GTTCCACTTG GGCGAACAGC AAATCATGAC 7801 TTATCTTCTA AATAAAATTT TAGTAAGTCT TGTCCTGGCA TATTATACAT TCCATCGATG 7861 TAGTCTTCAA CATTAACAAC TTTAAGTCCA GCAATTTGAG TTAAGGGTGT TGCTCTCAAT 7921 GATTTCATTA ATGGTTCAAT TTTTAATTTC TTTTCTTCTG GTTTAAAATT CAAGTTTAAA 7981 GTGAAAGTGT AATATGCACC CATTTCTTTA AATAAATCTT CTAAATAGTC TACTAATGTT 8041 TTATTTTGTT TTTTATAAAA TCAAGCAGCC TCTGCTATTA ATATAGAAGC TTGTATTCCA 8101 TCTTTATCTC TAGCTGAGTC ATCAATTACA TATCCATAAC TTTCTTCATA AGCAAAAACA 8161 AAATTTAATC CGTTATCTTC TTCTTTAGCA ATTTCTCTAC CCATTCATTT AAATCCAGTT 8221 AAAGTTTTTA CAATATTAAC TCCATATTTT TCATGAGCGA TTCTATCACC CAAATCACTT 8281 GTTACAAAAC TTGAATATAG AGCCGGATTT TTTGGAATGC TATTTAAGCG TTTTAGATTT 8341 GATAATTTTC AATCAATTAA AATTGGTCCT GTTTGATTTC CATCTAATCT TACAAAATGA 8401 CCATCATGTT TTATTGCCAT TCCAAATCTG TCAGCATCTG GGTCATTCAT AATAATAATA 8461 TCTGCATCAT GTTTAATACC ATATTCAAGC GGTATTTTTC ATGCAGGATC AAATTCTGGA 8521 TTTGGATTTA CAACATTTTT AAATGTTTCA TCTTCAAATG CATGCTCTTC AACCTCAATA 8581 ACGTTATATC CTGATTCACG TAATATTTTT GGGGTAAATT TAGTTCCTGT TCCATTAACT 8641 GCGCTAAAAA TAATTTTTAA ATCTTTTTTA GCTTCTTGCT CTTTTTTGTA GAATTCtcga 8701 cctcgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt 8761 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac 8821 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac 8881 tttggccgcg gctcgagggg g //
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pLKO.1-TRC质粒 pLK0.1-TRC载体应用举例
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