| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET37b, pet 37b, pET-37b(+) |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5932 bp |
| 5' 测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3' 测序引物序列: | T7t: 5'-GCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-Signal, N-CBD, N-S, N-Thrombin, N-EK, C-His |
| 载体抗性: | 卡那 |
| 表达宿主菌: | BL21(DE3) 、 Rosetta2(DE3)、C43(DE3) |
| 备注: | 载体含有N端纤维素结合结构域(N-CBD), 是分泌性蛋白表达载体。 |
| 产品目录号: | |
| 稳定性: | 瞬时表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-37b(+)载体 pET37b质粒基本信息
pET-37b(+)质粒图谱 pET37b载体图谱和pET-37b(+)载体序列 pET37b质粒序列多克隆位点信息
pET-37b(+)质粒 pET37b载体简介
pET-37b(+)质粒序列 pET37b载体序列
LOCUS pET-37b(+) 5932 bp DNA circular 23-MAY-2002 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers promoter complement(929..945) /vntifkey="30" /label=T7\promoter misc_signal complement(729..857) /vntifkey="87" /label=Signal\sequence primer_bind complement(386..730) /vntifkey="28" /label=CBD(cenA)\tag protein_bind complement(291..335) /vntifkey="31" /label=S\tag protein_bind complement(150..173) /vntifkey="31" /label=His\tag terminator complement(26..72) /vntifkey="43" /label=T7\terminator CDS 1336..2415 /vntifkey="4" /label=lac\I rep_origin complement(3849..3849) /vntifkey="33" /label=ColE1\pBR322 CDS 4558..5370 /vntifkey="4" /label=kan\sequence rep_origin complement(5466..5921) /vntifkey="33" /label=f1\origin protein_bind complement(351..368) /vntifkey="31" /label=thrombin protein_bind complement(249..260) /vntifkey="31" /label=factor\Xa misc_feature complement(249..249) /vntifkey="21" /label=LIC\site CDS complement(902..926) /vntifkey="4" /label=lac\operator BASE COUNT 1353 a 1555 c 1669 g 1355 t ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggccagagga 241 gagttagagc gaccctcaat accggagcca ccaccggtac ccagatctgg gctgtccatg 301 tgctggcgtt cgaatttagc agcagcggtt tctttcatac caattgcagt actaccgcgt 361 ggcaccagac ccgcggaacc tggtgccgtg gggctggtcg tcggcacggt gcccgtgcag 421 gtggtgccgt tgagcgagaa cgacgccggc gtcgggttgg acccggccca cgagccgttg 481 aacccgaacg acgccgtgcc gccggtcggg atgctgccgt tccagggcag gctggtgacg 541 gagacctggc cgccgttggt cgaggcggtg ccgttccaca gctgctggat acgctggcct 601 gcggtgtagg tccagtcgag cttccacgac gagacggggt cgccgaggtt ggtgatcgtg 661 acgttggcgc cgaagccgcc gggccactgg ttggtgacgg cgtagtcgac gcggcagccg 721 ggactagcgg cctgggcggg cagcacgacc gtggcgccga cgacgagcgt cgccgccgcg 781 gcggcgagcg tcgtgcgcag agcggtgcgg gcgccgcggg ccgggtggcc gggtgcgggc 841 gtggtacgag ggtccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag 901 gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga 961 gatcgatctc gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg 1021 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg 1081 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt 1141 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc 1201 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga gatcccggac 1261 accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa 1321 ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc 1381 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg 1441 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa 1501 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg 1561 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg 1621 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc 1681 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct 1741 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc 1801 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc 1861 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt 1921 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa 1981 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag 2041 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc 2101 gccattaccg agtccgggct gcgcgttggt gcggacatct cggtagtggg atacgacgat 2161 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg 2221 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc 2281 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa 2341 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 2401 ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc 2461 gggatctcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 2521 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 2581 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat 2641 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac 2701 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggcccc 2761 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 2821 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 2881 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 2941 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 3001 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 3061 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 3121 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 3181 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 3241 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 3301 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 3361 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 3541 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 3601 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 4261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 4501 ttttggtcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg 4561 agccatattc aacgggaaac gtcttgctct aggccgcgat taaattccaa catggatgct 4621 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat 4681 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt 4741 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt 4801 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc 4861 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt 4921 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt 4981 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt 5041 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa 5101 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt 5161 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga 5221 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct 5281 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg 5341 cagtttcatt tgatgctcga tgagtttttc taagaattaa ttcatgagcg gatacatatt 5401 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 5461 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 5521 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 5581 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 5641 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 5701 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 5761 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 5821 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 5881 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca //
