| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pACYCDuet1, pACYCDuet 1 |
| 质粒类型: | 大肠杆菌蛋白双基因表达载体，有两个多克隆位点 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4008 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; |
| 3' 测序引物: | T7t |
| 3' 测序引物序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-His, C-S |
| 载体抗性: | 氯霉素 |
| 备注: |
pACYCDuet-1 is designed for the coexpression of two target genes. pACYCDuet-1 carries the P15A replicon, lacI gene and chloramphenicol resistance gene.
|
| 产品目录号: | 71147-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
买家导航
pACYCDuet1载体基本信息
pACYCDuet1载体图谱质粒图谱和多克隆位点信息
pACYCDuet1载体简介
pACYCDue-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179- 3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3).
pACYCDuet1载体序列
LOCUS pACYCDuet-1 4008 bp DNA Circular SYN
DEFINITION pACYCDuet-1
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..4008
/organism="pACYCDuet-1"
/mol_type="other DNA"
misc_feature 2..29
/label="lacO"
promoter 214..232
/label="T7_promoter"
misc_feature 232..259
/label="lacO"
misc_feature complement(448..466)
/label="T7_Terminal_primer"
gene complement(729..1388)
/label="CAT/CamR (variant)"
/gene="CAT/CamR (variant)"
CDS complement(729..1388)
/label="ORF frame 2"
rep_origin 1750..2661
/label="p15A_origin"
misc_feature complement(2786..3877)
/label="lacI"
CDS complement(2786..3745)
/label="ORF frame 3"
CDS complement(3618..158)
/label="ORF frame 2"
promoter 3992..2
/label="T7_promoter"
ORIGIN
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct
721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc
901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca
1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct
1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc
1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc
1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc
1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt
1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac
1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga
2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga
2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag
2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata
//
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