| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pCDFDuet1, pCDFDuet 1 |
| 质粒类型: | 大肠杆菌蛋白双表达载体，有两个多克隆位点 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3781 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; |
| 3' 测序引物: | T7t |
| 3' 测序引物序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-His, C-S |
| 载体抗性: | streptomycin |
| 备注: |
pCDFDuet-1 is designed for the coexpression of two target genes. pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA).
|
| 产品目录号: | 71340-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
买家导航
pCDFDuet-1载体 pCDFDuet1质粒基本信息
pCDFDuet-1质粒图谱 pCDFDuet1载体图谱和pCDFDuet-1载体序列 pCDFDuet1质粒序列多克隆位点信息
pCDFDuet-1质粒 pCDFDuet1载体简介
pCDFDue-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.
pCDFDuet-1质粒序列 pCDFDuet1载体序列
LOCUS pCDFDuet 3781 bp DNA Circular SYN
DEFINITION pCDFDuet
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3781
/organism="pCDFDuet"
/mol_type="other DNA"
misc_feature 2..29
/label="lacO"
promoter 214..232
/label="T7_promoter"
misc_feature 232..259
/label="lacO"
misc_feature complement(448..466)
/label="T7_Terminal_primer"
terminator 467..502
/label="T7_terminator"
gene complement(680..1471)
/label="spect (variant)"
/gene="spect (variant)"
CDS complement(680..1471)
/label="ORF frame 1"
CDS complement(680..1471)
/label="ORF frame 1"
promoter complement(1513..1541)
/label="AmpR_promoter"
misc_feature complement(2559..3650)
/label="lacI"
CDS complement(2559..3518)
/label="ORF frame 3"
CDS complement(2559..3518)
/label="ORF frame 3"
CDS complement(3391..158)
/label="ORF frame 3"
CDS complement(3391..158)
/label="ORF frame 3"
promoter 3765..2
/label="T7_promoter"
ORIGIN
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
601 aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc
661 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
721 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
781 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
841 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
901 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
961 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
1021 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
1081 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
1141 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
1201 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
1261 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
1321 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
1381 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
1441 tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca
1501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
1561 aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
1621 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
1681 cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
1741 aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
1801 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct
1861 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac
1921 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt
1981 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc
2041 ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc
2101 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
2161 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
2221 agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag
2281 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat
2341 cctttgatct tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta
2401 gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg
2461 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
2521 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
2581 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
2641 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
2701 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
2761 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
2821 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
2881 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
2941 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
3001 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
3061 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
3121 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
3181 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
3241 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
3301 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
3361 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
3421 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
3481 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
3541 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
3601 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
3661 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
3721 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat
3781 a
//
DEFINITION pCDFDuet
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3781
/organism="pCDFDuet"
/mol_type="other DNA"
misc_feature 2..29
/label="lacO"
promoter 214..232
/label="T7_promoter"
misc_feature 232..259
/label="lacO"
misc_feature complement(448..466)
/label="T7_Terminal_primer"
terminator 467..502
/label="T7_terminator"
gene complement(680..1471)
/label="spect (variant)"
/gene="spect (variant)"
CDS complement(680..1471)
/label="ORF frame 1"
CDS complement(680..1471)
/label="ORF frame 1"
promoter complement(1513..1541)
/label="AmpR_promoter"
misc_feature complement(2559..3650)
/label="lacI"
CDS complement(2559..3518)
/label="ORF frame 3"
CDS complement(2559..3518)
/label="ORF frame 3"
CDS complement(3391..158)
/label="ORF frame 3"
CDS complement(3391..158)
/label="ORF frame 3"
promoter 3765..2
/label="T7_promoter"
ORIGIN
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
601 aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc
661 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
721 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
781 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
841 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
901 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
961 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
1021 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
1081 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
1141 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
1201 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
1261 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
1321 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
1381 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
1441 tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca
1501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
1561 aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
1621 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
1681 cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
1741 aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
1801 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct
1861 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac
1921 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt
1981 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc
2041 ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc
2101 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
2161 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
2221 agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag
2281 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat
2341 cctttgatct tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta
2401 gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg
2461 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
2521 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
2581 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
2641 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
2701 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
2761 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
2821 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
2881 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
2941 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
3001 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
3061 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
3121 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
3181 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
3241 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
3301 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
3361 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
3421 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
3481 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
3541 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
3601 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
3661 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
3721 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat
3781 a
//
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pCDFDuet-1质粒 pCDFDuet1载体应用举例
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