| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pRSFDuet1, pRSFDuet 1 |
| 质粒类型: | 大肠杆菌蛋白双表达载体，有两个多克隆位点 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3829 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; |
| 3' 测序引物: | T7t |
| 3' 测序引物序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-His, C-S |
| 载体抗性: | Kanamycin |
| 备注: |
pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene.
|
| 产品目录号: | 71341-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
买家导航
pRSFDuet-1载体 pRSFDuet1质粒基本信息
pRSFDuet-1质粒图谱 pRSFDuet1载体图谱和pRSFDuet-1载体序列 pRSFDuet1质粒序列多克隆位点信息
pRSFDuet-1质粒 pRSFDuet1载体简介
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pCDFDuet™-1 (Cat. Np. 71340-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.
pRSFDuet-1质粒序列 pRSFDuet1载体序列
LOCUS pRSFDuet-1 3829 bp DNA Circular SYN
DEFINITION pRSFDuet-1
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3829
/organism="pRSFDuet-1"
/mol_type="other DNA"
misc_feature 2..29
/label="lacO"
promoter 214..232
/label="T7_promoter"
misc_feature 232..259
/label="lacO"
misc_feature complement(448..466)
/label="T7_Terminal_primer"
gene complement(739..1554)
/label="KanR2"
/gene="KanR2"
CDS complement(739..1554)
/label="ORF frame 2"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
promoter complement(1596..1624)
/label="AmpR_promoter"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
CDS complement(2607..3566)
/label="ORF frame 3"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS complement(3439..158)
/label="ORF frame 3"
/translation="MRPQACRPAGAPSSNSDPGCGDDGDGCCPWYISLLKLNKIISTG
ELLSAHNSPIVSRINFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECET
SNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTV
VADWRCHLQSGPARAVANCRGD*"
promoter 3813..2
/label="T7_promoter"
/translation="MRPQACRPAGAPSSNSDPGCGDDGDGCCPWYISLLKLNKIISTG
ELLSAHNSPIVSRINFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECET
SNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTV
VADWRCHLQSGPARAVANCRGD*"
ORIGIN
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt
661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca
1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt
1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
1621 tgaatgtatt tagaaaaata aacaaatagg catgcagcgc tcttccgctt cctcgctcac
1681 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggtg tcagctcact caaaagcggt
1741 aatacggtta tccacagaat caggggataa agccggaaag aacatgtgag caaaaagcaa
1801 agcaccggaa gaagccaacg ccgcaggcgt ttttccatag gctccgcccc cctgacgagc
1861 atcacaaaaa tcgacgctca agccagaggt ggcgaaaccc gacaggacta taaagatacc
1921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
1981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt
2041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2161 acgacttatc gccactggca gcagccattg gtaactgatt tagaggactt tgtcttgaag
2221 ttatgcacct gttaaggcta aactgaaaga acagattttg gtgagtgcgg tcctccaacc
2281 cacttacctt ggttcaaaga gttggtagct cagcgaacct tgagaaaacc accgttggta
2341 gcggtggttt ttctttattt atgagatgat gaatcaatcg gtctatcaag tcaacgaaca
2401 gctattccgt tactctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
2461 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
2521 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
2581 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
2701 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
2761 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
2821 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
2881 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
2941 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
3001 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
3061 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
3121 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
3181 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
3241 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
3301 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
3361 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
3421 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
3481 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
3541 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
3601 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
3661 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
3721 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
3781 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata
//
DEFINITION pRSFDuet-1
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3829
/organism="pRSFDuet-1"
/mol_type="other DNA"
misc_feature 2..29
/label="lacO"
promoter 214..232
/label="T7_promoter"
misc_feature 232..259
/label="lacO"
misc_feature complement(448..466)
/label="T7_Terminal_primer"
gene complement(739..1554)
/label="KanR2"
/gene="KanR2"
CDS complement(739..1554)
/label="ORF frame 2"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
promoter complement(1596..1624)
/label="AmpR_promoter"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
CDS complement(2607..3566)
/label="ORF frame 3"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS complement(3439..158)
/label="ORF frame 3"
/translation="MRPQACRPAGAPSSNSDPGCGDDGDGCCPWYISLLKLNKIISTG
ELLSAHNSPIVSRINFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECET
SNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTV
VADWRCHLQSGPARAVANCRGD*"
promoter 3813..2
/label="T7_promoter"
/translation="MRPQACRPAGAPSSNSDPGCGDDGDGCCPWYISLLKLNKIISTG
ELLSAHNSPIVSRINFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECET
SNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTV
VADWRCHLQSGPARAVANCRGD*"
ORIGIN
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt
661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca
1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt
1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
1621 tgaatgtatt tagaaaaata aacaaatagg catgcagcgc tcttccgctt cctcgctcac
1681 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggtg tcagctcact caaaagcggt
1741 aatacggtta tccacagaat caggggataa agccggaaag aacatgtgag caaaaagcaa
1801 agcaccggaa gaagccaacg ccgcaggcgt ttttccatag gctccgcccc cctgacgagc
1861 atcacaaaaa tcgacgctca agccagaggt ggcgaaaccc gacaggacta taaagatacc
1921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
1981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt
2041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2161 acgacttatc gccactggca gcagccattg gtaactgatt tagaggactt tgtcttgaag
2221 ttatgcacct gttaaggcta aactgaaaga acagattttg gtgagtgcgg tcctccaacc
2281 cacttacctt ggttcaaaga gttggtagct cagcgaacct tgagaaaacc accgttggta
2341 gcggtggttt ttctttattt atgagatgat gaatcaatcg gtctatcaag tcaacgaaca
2401 gctattccgt tactctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
2461 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
2521 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
2581 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
2701 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
2761 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
2821 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
2881 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
2941 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
3001 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
3061 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
3121 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
3181 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
3241 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
3301 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
3361 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
3421 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
3481 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
3541 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
3601 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
3661 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
3721 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
3781 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata
//
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