| 质粒名称: | Dendra2-H1-10 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 57723 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | Dendra2 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | H1,Mouse Histone H1,NM_008197.3 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | Dendra2 |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Dendra2-H1-10 was a gift from Michael Davidson (Addgene plasmid # 57723 ; http://n2t.net/addgene:57723 ; RRID:Addgene_57723) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Dendra2-H1-10 5242 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Localization: Nucleus/Histones, Excitation: 490 / 553, Emission: 507
/ 573.
ACCESSION .
VERSION .
KEYWORDS Dendra2-H1-10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5242)
TITLE Davidson Photoactivatable Fluorescent Proteins
REFERENCE 2 (bases 1 to 5242)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5242
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 1156..1848
/codon_start=1
/product="monomeric photoswitchable green-to-red
fluorescent protein from Dendronephthya"
/label=Dendra2
/note="mammalian codon-optimized"
/translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
SPLPSQVW"
polyA_signal 1970..2091
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2007..2026)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2061..2080
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2098..2553)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2235..2256)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2447..2466
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2580..2684
/gene="bla"
/label=AmpR promoter
promoter 2686..3043
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2709..2729)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2894..3029
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2956..2975
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3078..3872
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3132..3151)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3742..3761
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4060..4079)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4104..4151
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4480..5068
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4969..4988
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccgcc
541 accatgaccg agaactccac ctccgccccg gcggcgaagc ccaaacgggc caaggcttcc
601 aagaagtcca cggaccaccc caagtattca gacatgatcg tggctgctat ccaggcagag
661 aagaaccgtg ccggctcctc gcgccagtcc atccaaaagt atatcaagag ccactacaag
721 gtgggtgaga acgccgactc ccagatcaag ttgtccatca agcgcctagt gaccaccggt
781 gttctcaagc aaaccaaagg ggtgggcgcc tcggggtcct tcaggctggc caagggcgat
841 gagcccaaaa ggtcggtggc tttcaagaag accaagaagg aagtcaagaa agtggccact
901 ccaaagaagg cagccaagcc caagaaggct gcctccaaag ccccaagcaa gaaacccaaa
961 gccacccctg tcaagaaggc caagaagaag ccggctgcca cgcccaagaa agccaaaaag
1021 cccaaggttg tcaaagtcaa accagtcaag gcctccaaac ccaagaaggc caaaaccgtg
1081 aagcccaaag ccaagtcgag tgccaagagg gggagcaaga agaagtcggg aagcacggat
1141 ccaccggtcg ccaccatgaa caccccggga attaacctga tcaaggagga catgcgcgtg
1201 aaggtgcaca tggagggcaa cgtgaacggc cacgccttcg tgatcgaggg cgagggcaag
1261 ggcaagccct acgagggcac ccagaccgcc aacctgaccg tgaaggaggg cgcccccctg
1321 cccttcagct acgacatcct gaccaccgcc gtgcactacg gcaaccgggt gttcaccaag
1381 taccccgagg acatccccga ctacttcaag cagagcttcc ccgagggcta cagctgggag
1441 cgcaccatga ccttcgagga caagggcatc tgcaccatcc gcagcgacat cagcctggag
1501 ggcgactgct tcttccagaa cgtgcgcttc aagggcacca acttcccccc caacggcccc
1561 gtgatgcaga agaagaccct gaagtgggag cccagcaccg agaagctgca cgtgcgcgac
1621 ggcctgctgg tgggcaacat caacatggcc ctgctgctgg agggcggcgg ccactacctg
1681 tgcgacttca agaccaccta caaggccaag aaggtggtgc agctgcccga cgcccacttc
1741 gtggaccacc gcatcgagat cctgggcaac gacagcgact acaacaaggt gaagctgtac
1801 gagcacgccg tggcccgcta cagccccctg cccagccagg tgtggtaagc ggccgcgact
1861 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
1921 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
1981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
2041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
2101 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
2161 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
2221 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
2281 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
2341 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
2401 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
2461 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
2521 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
2581 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
2641 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
2701 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
2761 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
2821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
2881 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
2941 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
3001 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
3061 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
3121 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
3181 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3241 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3301 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3361 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3421 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3481 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3541 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3601 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
3661 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3721 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3781 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3841 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3901 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3961 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
4021 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
4081 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
4141 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
4201 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
4261 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
4321 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
4381 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
4441 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
4501 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
4561 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
4621 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
4681 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
4741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
4801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
4861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
4921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
4981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
5041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
5101 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
5161 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
5221 atagcccata tatggagttc cg
//
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