pCambia1391Z

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pCambia1391Z载体 pCambia 1391Z质粒基本信息

出品公司: CAMBIA
载体名称: pCAMBIA1391Z
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lac
载体大小: 11227 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: GUS、6xHis
载体抗性: 卡那霉素
筛选标记: 潮霉素
克隆菌株: HB101等菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

pCambia1391Z质粒图谱 pCambia 1391Z载体图谱和pCambia1391Z载体序列 pCambia 1391Z质粒序列多克隆位点信息

pCAMBIA1391Z载体图谱



pCAMBIA1391Z 多克隆位点

pCambia1391Z质粒 pCambia 1391Z载体简介

pCambia1391Z质粒序列 pCambia 1391Z载体序列

LOCUS       pCAMBIA1391Z          11227 bp ds-DNA    circular SYN 01-十二月-2013
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            hygromycin- and kanamycin-resistance and LacZ-GUS genes plus the 
            pUC9 MCS.
ACCESSION   AF234312
VERSION     .
KEYWORDS    pCAMBIA1391Z
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Cambia
  TITLE     Direct Submission
  JOURNAL   Exported 2014年6月2日 from SnapGene Viewer 1.5.3
            http://www.snapgene.com
COMMENT     The GenBank record was corrected by inserting a G at position 4743.
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     CDS             join(2..16,207..2024)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label="GUS"
                     /label="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /protein_id="
                     "                    
     intron          17..206
                     /label="cat1 intron"
                     /label="castor bean catalase intron, modified"
     CDS             2031..2048
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label="6xHis"
                     /translation="HHHHHH"
     terminator      2083..2335
                     /label="NOS terminator"
                     /label="nopaline synthase terminator and poly(A) signal"
     misc_feature    2357..2381
                     /label="RB T-DNA repeat"
                     /label="right border repeat from nopaline C58 T-DNA"
     CDS             3681..4310
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label="pVS1 StaA"                     
     CDS             4739..5812
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label="pVS1 RepA"
                     /label="
                     "
                     /protein_id="
                     "                     
     misc_feature    6416..6556
                     /label="bom"
                     /label="basis of mobility region from pBR322"
     rep_origin      complement(6742..7330)
                     /direction=LEFT
                     /label="ori"
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7417..8211)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label="KanR"
                     /label="confers resistance to kanamycin"
                     /protein_id="
                     "
     misc_feature    8636..8660
                     /label="LB T-DNA repeat"
                     /label="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8738..8912
                     /label="CaMV poly(A) signal"
                     /label="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8952..9977)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label="HygR"
                     /label="confers resistance to hygromycin"                     
     promoter        complement(10045..10722)
                     /label="CaMV 35S promoter (enhanced)"
                     /label="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        10936..10966
                     /label="lac promoter"
                     /label="promoter for the E. coli lac operon"
     protein_bind    10974..10990
                     /bound_moiety="lac repressor encoded by lacI"
                     /label="lac operator"
                     /label="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10998..11014
                     /label="M13 rev"
                     /label="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11010..11207
                     /codon_start=1
                     /gene="lacZ (fragment)"
                     /product="LacZ-alpha fragment of?beta-galactosidase"
                     /label="lacZ-alpha"
                     /protein_id="
                     "
                     /translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF
                     ASWRNSEEARTDRPSQQLRSL"
     misc_feature    11027..11062
                     /label="MCS"
                     /label="multiple cloning site from pUC9"
     primer_bind     complement(11063..11079)
                     /label="M13 fwd"
                     /label="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
      961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
     1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
     1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
     4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
     4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
     4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
     4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
     5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
     5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
     5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
     5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
     5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
     5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
     5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
     5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
     5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
     5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
     5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
     5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
     5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
     5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
     5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
     5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
     6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
     6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
     6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
     6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
     6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
     6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7381 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat
     7441 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
     7501 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
     7561 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
     7621 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
     7681 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
     7741 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
     7801 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
     7861 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
     7921 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
     7981 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
     8041 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
     8101 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
     8161 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
     8221 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
     8281 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
     8341 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
     8401 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
     8461 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
     8521 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
     8581 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
     8641 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
     8701 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt
     8761 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
     8821 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat
     8881 ctgggaacta ctcacacatt attatggaga aactcgagct tgtcgatcga cagatccggt
     8941 cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
     9001 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     9061 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     9121 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     9181 atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     9241 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     9301 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     9361 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     9421 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     9481 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     9541 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     9601 tcggccgcag cgatcgcatc catagcctcc gcgaccggtt gtagaacagc gggcagttcg
     9661 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     9721 ctctcgctaa actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     9781 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     9841 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     9901 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     9961 agttcaggct ttttcatatc tcattgcccc ccgggatctg cgaaagctcg agagagatag
    10021 atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta
    10081 tatagaggaa ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
    10141 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
    10201 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
    10261 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
    10321 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
    10381 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
    10441 tagacgagag tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg
    10501 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
    10561 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
    10621 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
    10681 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
    10741 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggcc
    10801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    10861 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    10921 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    10981 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttggctgcag
    11041 gtcgacggat ccccgggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    11101 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
    11161 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaag cctagggagg
    11221 agtccac
//

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