出品公司: | Clontech |
---|---|
载体名称: | pLVX-IRES-Puro |
质粒类型: | 慢病毒载体;哺乳动物细胞表达载体;双顺反子载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8140 bp |
5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen) |
3' 测序引物及序列: | IRES-R: CCTCACATTGCCAAAAGACG |
载体标签: | 无标签 |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
备注: |
pLVX-IRES-Puro载体以双顺反子的形式同时表达Puro抗性基因和目的基因; CMV启动子驱动目的基因的过表达; 克隆宿主菌为Stbl3,在30度条件下进行质粒扩增。 |
产品目录号: | 632183 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLVX-IRES-Puro载体质粒基本信息
pLVX-IRES-Puro质粒图谱载体图谱和pLVX-IRES-Puro载体序列质粒序列多克隆位点信息
pLVX-IRES-Puro质粒载体简介
pLVX-IRES-Puro质粒序列载体序列
LOCUS pLVX-IRES-Puro 8140 bp DNA circular 8-NOV-2009 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 632183,632186 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal misc_feature 2028..2151 /label=cPPT/CTS\(DNA\flap) promoter 2185..2787 /label=CMV\IE\Promoter misc_feature 4062..4653 /label=WPRE rep_origin complement(5961..6634) /label=pUC\origin CDS complement(6779..7775) /label=Amp(R) misc_binding 1303..1536 /label=RRE misc_binding 636..653 /label=PBS LTR 4856..5492 /label=3'\LTR misc_feature 2841..3448 /label=IRES CDS 3449..4048 /label=Puro(R) misc_feature 2803..2840 /label=MCS BASE COUNT 2082 a 2022 c 2094 g 1942 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 2821 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa 2881 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 2941 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 3001 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 3061 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 3121 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 3181 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 3241 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 3301 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 3361 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt 3421 gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc 3481 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 3541 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 3601 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 3661 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 3721 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 3781 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 3841 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 3901 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 3961 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4021 gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa caatcaacct ctggattaca 4081 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 4141 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 4201 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 4261 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 4321 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 4381 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 4441 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 4501 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 4561 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 4621 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 4681 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 4741 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc 4801 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga 4861 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca 4921 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 4981 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 5041 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 5101 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 5161 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 5221 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 5281 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 5341 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 5401 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 5461 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 5521 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt 5581 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 5641 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 5701 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 5761 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 5821 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 5881 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 5941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 6001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 6481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 6721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 6781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6841 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 6901 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 6961 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7021 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7081 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7141 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7201 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7261 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7321 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7381 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7441 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7501 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7561 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7621 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7681 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 7741 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 7801 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 7861 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 7921 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 7981 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 8041 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 8101 gaaattgtat ttgttaaata tgtactacaa acttagtagt //
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