LOCUS pLVX-TRE3G-IRES 8409 bp DNA circular 9-MAY-2012
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT 631362, 631351, 631354, 631257
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_feature 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 1303..1536
/label=RRE
misc_feature 2028..2151
/label=cPPT/CTS
misc_feature 3190..3693
/label=Ppgk
misc_feature 3714..4313
/label=Puro(R)
misc_feature 4331..4922
/label=WPRE
3'clip 5126..5762
/label=3'\LTR
misc_feature complement(6232..6902)
/label=pUC\ori
misc_feature 2210..2555
/label=pTRE3GV
misc_feature complement(7047..7907)
/label=Amp(R)
misc_feature 2585..3165
/label=IRES
misc_feature complement(7908..8012)
/label=pAmp(R)
misc_feature 3166..3189
/label=MCS-2
misc_feature 2556..2580
/label=MCS-1
BASE COUNT 2108 a 2117 c 2194 g 1991 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
2521 ctggagcaat tccacaacac ttttgtctta tacttggatc cgggcccgcg gccgccccgg
2581 ccggcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
2641 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
2701 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
2761 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
2821 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
2881 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
2941 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
3001 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
3061 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
3121 acggggacgt ggttttcctt tgaaaaacac gatgataata tggcccccgg gacgcgtcat
3181 atggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
3241 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
3301 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
3361 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
3421 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
3481 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
3541 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
3601 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
3661 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg
3721 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
3781 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
3841 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
3901 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
3961 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
4021 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
4081 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
4141 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
4201 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
4261 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacggggcg
4321 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
4381 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
4441 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
4501 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
4561 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
4621 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
4681 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
4741 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
4801 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
4861 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
4921 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
4981 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
5041 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
5101 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
5161 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
5221 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
5281 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
5341 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
5401 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
5461 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
5521 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
5581 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
5641 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
5701 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
5761 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
5821 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
5881 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
5941 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
6001 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
6061 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
6121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
6181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
6241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
6301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
6361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
6421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
6481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
6541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
6601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
6661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
6721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
6781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc
6841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
6901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
6961 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
7021 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
7081 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
7141 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
7201 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
7261 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
7321 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
7381 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
7441 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
7501 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
7561 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
7621 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
7681 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
7741 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
7801 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
7861 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
7921 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
7981 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
8041 gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa
8101 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
8161 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
8221 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt
8281 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
8341 tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat gtactacaaa
8401 cttagtagt
//