| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-TRE3G , pLVX TRE3G |
| 质粒类型: | 哺乳动物细胞慢病毒表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | TRE3GV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7819 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | 嘌呤霉素(Puromycin) |
| 备注: | 包含有Tet-On 3G反式激活蛋白，多西环素(doxycycline)诱导基因表达的慢病毒载体。 |
| 产品目录号: | 631191 |
| 稳定性: | / |
| 组成型: | 诱导型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLVX-TRE3G载体质粒基本信息
pLVX-TRE3G质粒图谱载体图谱和pLVX-TRE3G载体序列质粒序列多克隆位点信息
pLVX-TRE3G质粒载体简介
pLVX-TRE3G质粒序列载体序列
LOCUS pLVX-TRE3G 7819 bp DNA circular 31-MAY-2011
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No.631191, 631193,631187, 631189
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_feature 1..635
/label=5'\LTR
misc_feature 685..822
/label=Packaging\signal
misc_feature 1303..1536
/label=RRE
misc_feature 2028..2151
/label=cPPT/CTS
misc_feature 2600..3103
/label=pPGK
misc_feature 3124..3723
/label=Puro(R)
misc_feature 3741..4332
/label=WPRE
3'clip 4536..5172
/label=3'\LTR
misc_feature complement(5642..6312)
/label=pUC\ori
misc_feature 2556..2599
/label=MCS
misc_feature 2210..2555
/label=pTRE3G
misc_feature complement(6457..7317)
/label=Amp(R)
BASE COUNT 1973 a 1955 c 2040 g 1851 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
2521 ctggagcaat tccacaacac ttttgtctta tacttggatc cgggcccgcg gccgccccgg
2581 gacgcgtcat atggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
2641 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
2701 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
2761 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
2821 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
2881 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
2941 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
3001 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
3061 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
3121 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
3181 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
3241 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
3301 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
3361 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
3421 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
3481 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
3541 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
3601 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
3661 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
3721 tgacggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
3781 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
3841 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
3901 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
3961 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
4021 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
4081 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
4141 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
4201 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
4261 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
4321 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
4381 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
4441 ggtgggtttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
4501 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
4561 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
4621 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
4681 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
4741 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
4801 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
4861 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
4921 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
4981 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
5041 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
5101 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
5161 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
5221 tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta
5281 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
5341 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
5401 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
5461 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
5521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
5581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
5641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
5701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
5761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
5821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
5881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
5941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
6001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
6061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
6121 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
6181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
6241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
6301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
6361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
6421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
6481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
6541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
6601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
6661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
6721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
6781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
6841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
6901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
6961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
7021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
7081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
7141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
7201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
7261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
7321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
7381 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
7441 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca
7501 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
7561 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
7621 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag
7681 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
7741 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat
7801 gtactacaaa cttagtagt
//
pLVX-TRE3G载体图谱质粒图谱pdf版和pLVX-TRE3G载体序列质粒序列等相关资料下载
pLVX-TRE3G质粒载体应用举例
- 上一篇：pLVX-Tet3G
- 下一篇：pLVX-TRE3G-IRES
