| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-DD-AmCyan1 Reporter |
| 质粒类型: | 慢病毒载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 8494 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 青色荧光蛋白DD-AmCyan1 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系，293、CV-1、CHO等 |
| 备注: |
pLVX-DD-AmCyan1 Reporter载体没有启动子； DD-AmCyan1与AmCyan1相比，N端融合了ProteoTuner destabilization domain (DD)去稳定结构域， 导致AmCyan1在细胞内很快被蛋白酶降解，降低了背景荧光，是研究启动子活性的理想报告基因。 |
| 产品目录号: | 631749 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLVX-DD-AmCyan1 Reporter载体质粒基本信息
pLVX-DD-AmCyan1 Reporter质粒图谱载体图谱和pLVX-DD-AmCyan1 Reporter载体序列质粒序列多克隆位点信息
pLVX-DD-AmCyan1 Reporter质粒载体简介
pLVX-DD-AmCyan1 Reporter is a promoterless reporter vector that allows the functional analysis of different promoters
and promoter/enhancer combinations inserted into its multiple cloning site (MCS). The vector encodes the reporter protein
DD-AmCyan1, a ligand-dependent, destabilized cyan fluorescent protein that minimizes background fluorescence from
leaky promoters. A promoter must be cloned into the MCS, located upstream of the DD-AmCyan1 coding sequence.
Without the addition of a functional promoter, the vector will not express DD-AmCyan1.
Location of Features
5' LTR: 1–635
PBS (primer binding site): 636–653
Ψ (packaging signal): 685–822
RRE (Rev-response element): 1303–1536
cPPT/CTS (central polypurine tract/central termination sequence): 2028–2151
MCS (multiple cloning site): 2195–2234
DD (FKBP-L106P destabilization domain): 2247–2570
AmCyan1 (Anemonia majano cyan fluorescent protein): 2577–3263
PPGK (phosphoglycerate kinase promoter): 3274–3782
Puror(puromycin resistance gene): 3803–4402
WPRE (woodchuck hepatitis virus posttranscriptional regulatory element): 4416–5007
3' LTR: 5211–5847
pUC origin of replication: 6317–6987 (complementary)
Ampr(ampicillin resistance gene; β-lactamase): 7132–8128 (complementary)
