| 质粒名称: | pMK-aadA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 123558 |
| 存储实验室: | Scott Lenaghan, C. Neal Stewart |
|
载体骨架基本信息 |
|
| 载体名称: | pMK-T |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 合成生物学 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | aadA |
| 物种来源: | 合成的 |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMK-aadA was a gift from Scott Lenaghan & C. Neal Stewart (Addgene plasmid # 123558 ; http://n2t.net/addgene:123558 ; RRID:Addgene_123558) |
| 参考文献部分: | MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S. Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. 10.1104/pp.18.01220 PubMed 30679266 |
买家导航
pMKaadA载体基本信息
pMKaadA载体图谱质粒图谱和多克隆位点信息
pMKaadA载体简介
pMKaadA载体序列
LOCUS pMK-aadA 3122 bp ds-DNA circular SYN 27-JUN-2019
DEFINITION MoChlo kit.
ACCESSION .
VERSION .
KEYWORDS pMK-aadA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3122)
AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN,
Lenaghan S
TITLE MoChlo: A versatile modular cloning toolbox for chloroplast
biotechnology.
JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi:
10.1104/pp.18.01220.
PUBMED 30679266
REFERENCE 2 (bases 1 to 3122)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 27, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3122
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 278..300
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 292..309
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 293..309
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 320..339
/label=T7
/note="T7 promoter, forward primer"
promoter 320..338
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 343..354
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 394..1185
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MAEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
primer_bind complement(1328..1345)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1438..2026)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1518..1537)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2200..2994)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(2995..3099)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
361 aggccacgtg tcttgtccag agctcggtct caaatggcag aagcggtgat cgccgaagta
421 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
481 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
541 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
601 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
661 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
721 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
781 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
841 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
901 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
961 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
1021 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
1081 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
1141 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataagcttt gagaccggta
1201 cctggagcac aagactggcc tcatgggcct tcctttcact gcccgctttc cagtcgggaa
1261 acctgtcgtg ccagctgcat taacatggtc atagctgttt ccttgcgtat tgggcgctct
1321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta
1381 atgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
1441 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
1501 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
1561 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
1621 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
1681 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
1741 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
1801 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
1861 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
1921 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
1981 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2041 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2101 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2161 aatctaaagt atatatgagt aaacttggtc tgacagttat tagaaaaatt catccagcag
2221 acgataaaac gcaatacgct ggctatccgg tgccgcaatg ccatacagca ccagaaaacg
2281 atccgcccat tcgccgccca gttcttccgc aatatcacgg gtggccagcg caatatcctg
2341 ataacgatcc gccacgccca gacggccgca atcaataaag ccgctaaaac ggccattttc
2401 caccataatg ttcggcaggc acgcatcacc atgggtcacc accagatctt cgccatccgg
2461 catgctcgct ttcagacgcg caaacagctc tgccggtgcc aggccctgat gttcttcatc
2521 cagatcatcc tgatccacca ggcccgcttc catacgggta cgcgcacgtt caatacgatg
2581 tttcgcctga tgatcaaacg gacaggtcgc cgggtccagg gtatgcagac gacgcatggc
2641 atccgccata atgctcactt tttctgccgg cgccagatgg ctagacagca gatcctgacc
2701 cggcacttcg cccagcagca gccaatcacg gcccgcttcg gtcaccacat ccagcaccgc
2761 cgcacacgga acaccggtgg tggccagcca gctcagacgc gccgcttcat cctgcagctc
2821 gttcagcgca ccgctcagat cggttttcac aaacagcacc ggacgaccct gcgcgctcag
2881 acgaaacacc gccgcatcag agcagccaat ggtctgctgc gcccaatcat agccaaacag
2941 acgttccacc cacgctgccg ggctacccgc atgcaggcca tcctgttcaa tcatactctt
3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3121 ac
//
