| 质粒名称: | pSL-MS2-12X |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 27119 |
| 存储实验室: | Robert Singer |
|
载体骨架基本信息 |
|
| 载体名称: | pSL1180 |
| 载体出品公司: | Pharmacia |
| 空载体大小: | 3422 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | Stbl2 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | MS2 binding sites (12) |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | BglII(未破坏) |
| 5’测序引物 | M13_Rev |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSL-MS2-12X was a gift from Robert Singer (Addgene plasmid # 27119 ; http://n2t.net/addgene:27119 ; RRID:Addgene_27119) |
| 参考文献部分: | Localization of ASH1 mRNA particles in living yeast. Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM. Mol Cell. 1998 Oct . 2(4):437-45. 10.1016/S1097-2765(00)80143-4 PubMed 9809065 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSL-MS2-12X 4048 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSL-MS2-12X
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4048)
AUTHORS Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM
TITLE Localization of ASH1 mRNA particles in living yeast.
JOURNAL Mol Cell. 1998 Oct . 2(4):437-45.
PUBMED 9809065
REFERENCE 2 (bases 1 to 4048)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4048
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_RNA 94..112
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 133..151
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 203..221
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 242..260
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 312..330
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 351..369
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 421..439
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 460..478
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 530..548
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 569..587
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 639..657
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 678..696
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
primer_bind complement(954..971)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(954..970)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(963..985)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 1183..1638
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1270..1289)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1480..1501
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(1655..1674)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1774..1796
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1834..1852)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1920..2024
/gene="bla"
/label=AmpR promoter
CDS 2025..2885
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2243..2262)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3056..3644
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3545..3564
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3798..3815
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3932..3953
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3968..3998
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4006..4022
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4011..4033
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4030..4046
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4030..4046
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 gattacgaat tcgaatggcc atgggacgtc gacctgaggt aattataacc cgggccctat
61 atatggatcc taaggtacct aattgcctag aaaacatgag gatcacccat gtctgcaggt
121 cgactctaga aaacatgagg atcacccatg tctgcagtat tcccgggttc attagatcct
181 aaggtaccta attgcctaga aaacatgagg atcacccatg tctgcaggtc gactctagaa
241 aacatgagga tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa
301 ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actccagaaa acatgaggat
361 cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
421 acatgaggat cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct
481 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc
541 acccatgtct gcaggtcgac tctagaaaac atgaggatca cccatgtctg cagtattccc
601 gggttcatta gatcctaagg tacctaattg cctagaaaac atgaggatca cccatgtctg
661 caggtcgact ccagaaaaca tgaggatcac ccatgtctgc agtattcccg ggttcattag
721 atctgcgcgc gatcgatatc agcgctttaa atttgcgcat gctagctata gttctagagg
781 taccggttgt taacgttagc cggctacgta tactccggaa tattaatagg cctaggatgc
841 atatggcggc cgcctgcagc tggcgccatc gatacgcgta cgtcgcgacc gcggacatgt
901 acagagctcg agaagtacta gtggccacgt gggccgtgca ccttaagctt ggcactggcc
961 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
1021 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
1081 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
1141 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
1201 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
1261 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
1321 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
1381 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
1441 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
1501 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
1561 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
1621 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
1681 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
1741 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
1801 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
1861 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
1921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
1981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
2041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
2101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
2161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
2221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
2281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
2341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
2401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
2461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
2521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
2581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
2641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
2701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
2761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
2821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
2881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
2941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
3001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
3061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
3121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
3181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
3241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
3301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
3361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
3421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
3481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
3541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
3601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
3661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
3721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
3781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
3841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
3901 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
3961 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
4021 aatttcacac aggaaacagc tatgacat
//
