| 质粒名称: | pT2K-CAGGS-T2TP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 114725 |
| 存储实验室: | Martine Roussel |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGGS-T2TP |
| 载体出品公司: | Koichi Kawakami |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Tol2 transposase |
| 物种来源: | Medaka fish (Oryzias latipes) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pT2K-CAGGS-T2TP was a gift from Martine Roussel (Addgene plasmid # 114725 ; http://n2t.net/addgene:114725 ; RRID:Addgene_114725) |
| 参考文献部分: | Novel MYC-driven medulloblastoma models from multiple embryonic cerebellar cells. Kawauchi D, Ogg RJ, Liu L, Shih DJH, Finkelstein D, Murphy BL, Rehg JE, Korshunov A, Calabrese C, Zindy F, Phoenix T, Kawaguchi Y, Gronych J, Gilbertson RJ, Lichter P, Gajjar A, Kool M, Northcott PA, Pfister SM, Roussel MF. Oncogene. 2017 Sep 14;36(37):5231-5242. doi: 10.1038/onc.2017.110. Epub 2017 May 15. 10.1038/onc.2017.110 PubMed 28504719 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pT2K-CAGGS-T2TP 6992 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expression in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pT2K-CAGGS-T2TP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6992)
AUTHORS Kawauchi D, Ogg RJ, Liu L, Shih DJH, Finkelstein D, Murphy BL, Rehg
JE, Korshunov A, Calabrese C, Zindy F, Phoenix T, Kawaguchi Y,
Gronych J, Gilbertson RJ, Lichter P, Gajjar A, Kool M, Northcott PA,
Pfister SM, Roussel MF
TITLE Novel MYC-driven medulloblastoma models from multiple embryonic
cerebellar cells.
JOURNAL Oncogene. 2017 Sep 14;36(37):5231-5242. doi: 10.1038/onc.2017.110.
Epub 2017 May 15.
PUBMED 28504719
REFERENCE 2 (bases 1 to 6992)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6992
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 5..384
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 662..1678
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1602..1624
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1686..1705
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind complement(4014..4033)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 4079..4134
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(4133..4152)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(4495..4511)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4495..4511)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4508..4530)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4519..4535
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4543..4573)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4588..4609
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4668..4863
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 4714..4849
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4776..4795
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 4869..5003
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4906..4925)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4960..4979
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5071..5088)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5242..5830)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5322..5341)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6001..6861)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6624..6643
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6862..6966)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggsgcgc gccrggsggg gcggggsggg gcgrggggsg
541 gggsggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
1261 ggggccgcct cgggccgggg rgggctcggg ggaggggcgc ggcggccccc ggagcgccgg
1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgaggt
1741 cgagtcacat ctattaccac aatgcacagc accttgacct ggaaattagg gaaattataa
1801 cagtcaatca gtggaagaaa atggaggaag tatgtgattc atcagcagct gcgagcagca
1861 cagtccaaaa tcagccacag gatcaagagc acccgtggcc gtatcttcgc gaattctttt
1921 ctttaagtgg tgtaaataaa gattcattca agatgaaatg tgtcctctgt ctcccgctta
1981 ataaagaaat atcggccttc aaaagttcgc catcaaacct aaggaagcat attgagagaa
2041 tgcacccaaa ttacctcaaa aactactcta aattgacagc acagaagaga aagatcggga
2101 cctccaccca tgcttccagc agtaagcaac tgaaagttga ctcagttttc ccagtcaaac
2161 atgtgtctcc agtcactgtg aacaaagcta tattaaggta catcattcaa ggacttcatc
2221 ctttcagcac tgttgatctg ccatcattta aagagctgat tagtacactg cagcctggca
2281 tttctgtcat tacaaggcct actttacgct ccaagatagc tgaagctgct ctgatcatga
2341 aacagaaagt gactgctgcc atgagtgaag ttgaatggat tgcaaccaca acggattgtt
2401 ggactgcacg tagaaagtca ttcattggtg taactgctca ctggatcaac cctggaagtc
2461 ttgaaagaca ttccgctgca cttgcctgca aaagattaat gggctctcat acttttgagg
2521 tactggccag tgccatgaat gatatccact cagagtatga aatacgtgac aaggttgttt
2581 gcacaaccac agacagtggt tccaacttta tgaaggcttt cagagttttt ggtgtggaaa
2641 acaatgatat cgagactgag gcaagaaggt gtgaaagtga tgacactgat tctgaaggct
2701 gtggtgaggg aagtgatggt gtggaattcc aagatgcctc acgagtcctg gaccaagacg
2761 atggcttcga attccagcta ccaaaacatc aaaagtgtgc ctgtcactta cttaacctag
2821 tctcaagcgt tgatgcccaa aaagctctct caaatgaaca ctacaagaaa ctctacagat
2881 ctgtctttgg caaatgccaa gctttatgga ataaaagcag ccgatcggct ctagcagctg
2941 aagctgttga atcagaaagc cggcttcagc ttttaaggcc aaaccaaacg cggtggaatt
3001 caacttttat ggctgttgac agaattcttc aaatttgcaa agaagcagga gaaggcgcac
3061 ttcggaatat atgcacctct cttgaggttc caatgtttaa tccagcagaa atgctgttct
3121 tgacagagtg ggccaacaca atgcgtccag ttgcaaaagt actcgacatc ttgcaagcgg
3181 aaacgaatac acagctgggg tggctgctgc ctagtgtcca tcagttaagc ttgaaacttc
3241 agcgactcca ccattctctc aggtactgtg acccacttgt ggatgcccta caacaaggaa
3301 tccaaacacg attcaagcat atgtttgaag atcctgagat catagcagct gccatccttc
3361 tccctaaatt tcggacctct tggacaaatg atgaaaccat cataaaacga ggcatggact
3421 acatcagagt gcatctggag cctttggacc acaagaagga attggccaac agttcatctg
3481 atgatgaaga ttttttcgct tctttgaaac cgacaacaca tgaagccagc aaagagttgg
3541 atggatatct ggcctgtgtt tcagacacca gggagtctct gctcacgttt cctgctattt
3601 gcagcctctc tatcaagact aatacacctc ttcccgcatc ggctgcctgt gagaggcttt
3661 tcagcactgc aggattgctt ttcagcccca aaagagctag gcttgacact aacaattttg
3721 agaatcagct tctactgaag ttaaatctga ggttttacaa ctttgagtag cgtgtactgg
3781 cattagattg tctgtcttat agtttgataa ttaaatacaa acagttctaa agcaggataa
3841 aaccttgtat gcatttcatt taatgttttt tgagattaaa agcttaaaca agaaaaaaaa
3901 aaaaaaaaaa aaaaaaaagt actagtcgag gaattcactc ctcaggtgca ggctgcctat
3961 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
4021 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
4081 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
4141 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
4201 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
4261 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
4321 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
4381 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
4441 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
4501 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
4561 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
4621 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag
4681 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
4741 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
4801 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
4861 aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
4921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
4981 atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
5041 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
5101 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
5161 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
5221 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
5281 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
5341 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
5401 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
5461 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
5521 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
5581 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
5641 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
5701 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
5761 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
5821 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
5881 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
5941 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
6001 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
6061 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
6121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
6181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
6241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
6301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
6361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
6421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
6481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
6541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
6601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
6661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
6721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
6781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
6841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
6901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
6961 tccgcgcaca tttccccgaa aagtgccacc tg
//
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