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pDendra2-C载体 pDendra2C质粒基本信息
pDendra2-C质粒图谱 pDendra2C载体图谱和pDendra2-C载体序列 pDendra2C质粒序列多克隆位点信息
pDendra2-C质粒 pDendra2C载体简介
pDendra2-C质粒序列 pDendra2C载体序列
LOCUS pDendra2-C 4716 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for fusing Dendra2 to the N-terminus of a partner protein.
ACCESSION .
VERSION .
KEYWORDS pDendra2-C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4716)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, December 14, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4716
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1302
/codon_start=1
/product="monomeric photoswitchable green-to-red
fluorescent protein from Dendronephthya"
/note="Dendra2"
/note="mammalian codon-optimized"
/translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
SPLPSQVW"
misc_feature 1328..1380
/note="MCS"
/note="multiple cloning site of fluorescent protein
plasmids"
misc_feature 1389..1399
/note="stop codons"
/note="stop codons in all three reading frames"
polyA_signal 1504..1625
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1632..2087)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2114..2218
/gene="bla"
/note="AmpR promoter"
promoter 2220..2577
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2428..2563
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2612..3406
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3638..3685
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4014..4602
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatgaacac cccgggaatt aacctgatca aggaggacat gcgcgtgaag
661 gtgcacatgg agggcaacgt gaacggccac gccttcgtga tcgagggcga gggcaagggc
721 aagccctacg agggcaccca gaccgccaac ctgaccgtga aggagggcgc ccccctgccc
781 ttcagctacg acatcctgac caccgccgtg cactacggca accgggtgtt caccaagtac
841 cccgaggaca tccccgacta cttcaagcag agcttccccg agggctacag ctgggagcgc
901 accatgacct tcgaggacaa gggcatctgc accatccgca gcgacatcag cctggagggc
961 gactgcttct tccagaacgt gcgcttcaag ggcaccaact tcccccccaa cggccccgtg
1021 atgcagaaga agaccctgaa gtgggagccc agcaccgaga agctgcacgt gcgcgacggc
1081 ctgctggtgg gcaacatcaa catggccctg ctgctggagg gcggcggcca ctacctgtgc
1141 gacttcaaga ccacctacaa ggccaagaag gtggtgcagc tgcccgacgc ccacttcgtg
1201 gaccaccgca tcgagatcct gggcaacgac agcgactaca acaaggtgaa gctgtacgag
1261 cacgccgtgg cccgctacag ccccctgccc agccaggtgt ggtccggcga cagcggcgtg
1321 tacaagactc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
1381 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
1441 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
1501 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
1561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
1621 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1681 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
1741 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
1801 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
1861 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
1921 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
1981 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2041 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
2101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
2161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
2221 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
2281 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
2341 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
2401 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
2461 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
2521 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
2581 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
2641 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
2701 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
2761 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
2821 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
2881 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
2941 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
3001 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
3061 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
3121 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
3181 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
3241 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
3301 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
3361 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
3421 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
3481 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
3541 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
3601 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
3661 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
3721 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
3781 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
3841 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
3901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
3961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
4021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
4081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
4141 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
4201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
4261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
4321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
4381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
4441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
4501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
4561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
4621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
4681 cctgattctg tggataaccg tattaccgcc atgcat
//
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