载体名称: | pCP20 |
---|---|
质粒类型: | 基因敲除载体 |
高拷贝/低拷贝: | -- |
克隆方法: | -- |
启动子: | -- |
载体大小: | 9332 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pCP20载体质粒基本信息
pCP20质粒图谱载体图谱和pCP20载体序列质粒序列多克隆位点信息
pCP20质粒载体简介
pCP20质粒序列载体序列
LOCUS pCP20 9332 bp DNA circular SYN 13-JUN-2024 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9332) AUTHORS Ming TITLE Direct Submission JOURNAL Exported Jun 13, 2024 from SnapGene 6.0.2 to Vector NTI(R) format https://www.biofeng.com COMMENT VNTDATE|1060041600| COMMENT VNTDBDATE|1060041600| COMMENT VNTNAME|pCP20| COMMENT VNTAUTHORNAME|Ming| FEATURES Location/Qualifiers CDS complement(561..1220) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" promoter complement(1221..1323) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" rep_origin 1875..2097 /label=pSC101 ori /note="low-copy replication origin that requires the Rep101 protein" CDS 2145..3095 /codon_start=1 /gene="rep101 encoding an A56V mutation" /product="temperature-sensitive version of the RepA protein needed for replication with the pSC101 origin (Armstrong et al., 1984)" /label=Rep101(Ts) CDS complement(4557..5417) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(5418..5522) /gene="bla" /label=AmpR promoter CDS complement(6556..7827) /codon_start=1 /product="site-specific recombinase" /label=FLP /note="FLP is a site-specific recombinase from Saccharomyces cerevisiae. Recombination occurs at FRT sequences." RBS 7831..7839 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 7928..8641 /codon_start=1 /gene="cIts" /product="temperature-sensitive variant of the phage lambda repressor" /label=lambda repressor (ts) /note="thermosensitivity is conferred by the A67T mutation" ORIGIN 1 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca 61 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact 121 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg 181 gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcgcc 241 acaggtgcgg ttgctggcgc taaccgtttt tatcaggctc tgggaggcag aataaatgat 301 catatcgtca attattacct ccacggggag agcctgagca aactggcctc aggcatttga 361 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata 421 agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt 481 ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac 541 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat 601 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc 661 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg 721 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg 781 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg 841 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca 901 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca 961 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc 1021 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg 1081 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact 1141 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat 1201 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa 1261 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga 1321 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca 1381 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg 1441 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt 1501 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg 1561 caaaagcacc gccggacatc agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 1621 ccgggggact gttgggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag 1681 cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa 1741 aaggaatatt cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt 1801 aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa 1861 ctgactaaag tagtgagtta tacacagggc tgggatctat tctttttatc tttttttatt 1921 ctttctttat tctataaatt ataaccactt gaatataaac aaaaaaaaca cacaaaggtc 1981 tagcggaatt tacagagggt ctagcagaat ttacaagttt tccagcaaag gtctagcaga 2041 atttacagat acccacaact caaaggaaaa ggactagtaa ttatcattga ctagcccatc 2101 tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct gaattagttg 2161 ttttcaaagc aaatgaacta gcgattagtc gctatgactt aacggagcat gaaaccaagc 2221 taattttatg ctgtgtggca ctactcaacc ccacgattga aaaccctaca aggaaagaac 2281 ggacggtatc gttcacttat aaccaatacg ttcagatgat gaacatcagt agggaaaatg 2341 cttatggtgt attagctaaa gcaaccagag agctgatgac gagaactgtg gaaatcagga 2401 atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag ttctcaagcg 2461 aaaaattaga attagttttt agtgaagaga tattgcctta tcttttccag ttaaaaaaat 2521 tcataaaata taatctggaa catgttaagt cttttgaaaa caaatactct atgaggattt 2581 atgagtggtt attaaaagaa ctaacacaaa agaaaactca caaggcaaat atagagatta 2641 gccttgatga atttaagttc atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc 2701 ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc aatatgaaat 2761 tggtggttga taagcgaggc cgcccgactg atacgttgat tttccaagtt gaactagata 2821 gacaaatgga tctcgtaacc gaacttgaga acaaccagat aaaaatgaat ggtgacaaaa 2881 taccaacaac cattacatca gattcctacc tacataacgg actaagaaaa acactacacg 2941 atgctttaac tgcaaaaatt cagctcacca gttttgaggc aaaatttttg agtgacatgc 3001 aaagtaagta tgatctcaat ggttcgttct catggctcac gcaaaaacaa cgaaccacac 3061 tagagaacat actggctaaa tacggaagga tctgaggttc ttatggctct tgtatctatc 3121 agtgaagcat caagactaac aaacaaaagt agaacaactg ttcaccgtta catatcaaag 3181 ggaaaactgt ccatatgcac agatgaaaac ggtgtaaaaa agatagatac atcagagctt 3241 ttacgagttt ttggtgcatt taaagctgtt caccatgaac agatcgacaa tgtaacagat 3301 gaacagcatg taacacctaa tagaacaggt gaaaccagta aaacaaagca actagaacat 3361 gaaattgaac acctgagaca acttgttaca gctcaacagt cacacataga cagcctgaaa 3421 caggcgatgc tgcttatcga atcaaagctg ccgacaacac gggagccagt gacgcctccc 3481 gtggggaaaa aatcatggca attctggaag aaatagcgcc tgtttcgttt caggcaggtt 3541 atcagggagt gtcagcgtcc tgcggttctc cggggcgttc gggtcatgca gcccgtaatg 3601 gtgatttacc agcgtctgcc aggcatcaat tctaggcctg tctgcgcggt cgtagtacgg 3661 ctggaggcgt tttccggtct gtagctccat gttcggaatg acaaaattca gctcaagccg 3721 tcccttgtcc tggtgctcca cccacaggat gctgtactga tttttttcga gaccgggcat 3781 cagtacacgc tcaaagctcg ccatcacttt ttcacgtcct cccggcggca gctccttctc 3841 cgcgaacgac agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc 3901 ccggacttct tccggattac cctgaagcac cgttgcgcct tcgcggttac gctccctccc 3961 cagcaggtaa tcaaccggac cactgccacc accttttccc ctggcatgaa atttaactat 4021 catcccgcgc cccctgttcc ctgacagcca gacgcagccg gcgcagctca tccccgatgg 4081 ccatcagtgc ggccaccacc tgaacccggt caccggaaga ccactgcccg ctgttcacct 4141 tacgggctgt ctgattcagg ttatttccga tggcggccag ctgacgcagt aacggcggtg 4201 ccagtgtcgg cagttttccg gaacgggcaa ccggctcccc caggcagacc cgccgcatcc 4261 ataccgccag ttgtttaccc tcacagcgtt caagtaaccg ggcatgttca tcatcagtaa 4321 cccgtattgt gagcatcctc tcgcgtttca tcggtatcat taccccatga acagaaatcc 4381 cccttacacg gaggcatcag tgactaaacg gggtctgacg ctcagtggaa cgaaaactca 4441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 4501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 4561 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 4621 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 4681 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 4741 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 4801 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 4861 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 4921 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 4981 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 5041 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 5101 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 5161 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 5221 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 5281 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 5341 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 5401 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 5461 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 5521 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 5581 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg 5641 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg 5701 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg 5761 cttcctgtcg gccctcattc gtgcgctcta ggatcctcta cgccggacgc atcgtggccg 5821 gcatcaccgg cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg 5881 aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta 5941 ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg 6001 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg 6061 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag 6121 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca 6181 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg 6241 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt 6301 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa 6361 tccggtgaga atggcagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac 6421 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc 6481 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta 6541 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc 6601 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag 6661 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc 6721 ctttgatatt ggatcatatg catagtaccg agaaactagt gcgaagtagt gatcaggtat 6781 tgctgttatc tgatgagtat acgttgtcct ggccacggca gaagcacgct tatcgctcca 6841 atttcccaca acattagtca actccgttag gcccttcatt gaaagaaatg aggtcatcaa 6901 atgtcttcca atgtgagatt ttgggccatt ttttatagca aagattgaat aaggcgcatt 6961 tttcttcaaa gctttattgt acgatctgac taagttatct tttaataatt ggtattcctg 7021 tttattgctt gaagaattgc cggtcctatt tactcgtttt aggactggtt cagaattcct 7081 caaaaattca tccaaatata caagtggatc gatcctaccc cttgcgctaa agaagtatat 7141 gtgcctacta acgcttgtct ttgtctctgt cactaaacac tggattatta ctcccagata 7201 cttattttgg actaatttaa atgatttcgg atcaacgttc ttaatatcgc tgaatcttcc 7261 acaattgatg aaagtagcta ggaagaggaa ttggtataaa gtttttgttt ttgtaaatct 7321 cgaagtatac tcaaacgaat ttagtatttt ctcagtgatc tcccagatgc tttcaccctc 7381 acttagaagt gctttaagca tttttttact gtggctattt cccttatctg cttcttccga 7441 tgattcgaac tgtaattgca aactacttac aatatcagtg atatcagatt gatgtttttg 7501 tccatagtaa ggaataattg taaattccca agcaggaatc aatttcttta atgaggcttc 7561 cagaattgtt gctttttgcg tcttgtattt aaactggagt gatttattga caatatcgaa 7621 actcagcgaa ttgcttatga tagtattata gctcatgaat gtggctctct tgattgctgt 7681 tccgttatgt gtaatcatcc aacataaata ggttagttca gcagcacata atgctatttt 7741 ctcacctgaa ggtctttcaa acctttccac aaactgacga acaagcacct taggtggtgt 7801 tttacataat atatcaaatt gtggcataca acctccttag tacatgcaac cattatcacc 7861 gccagaggta aaatagtcaa cacgcacggt gttagatatt tatcccttgc ggtgatagat 7921 ttaacgtatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg 7981 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc 8041 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt 8101 aaatgcttat aacgccgcat tgcttacaaa aattctcaaa gttagcgttg aagaatttag 8161 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact 8221 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctaa 8281 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag 8341 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa 8401 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg 8461 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga 8521 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga 8581 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggctg 8641 atcggcaagg tgttctggtc ggcgcatagc tgataacaat tgagcaagaa tctgcatttc 8701 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac 8761 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa 8821 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac 8881 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat 8941 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag 9001 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac 9061 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata 9121 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc 9181 atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat 9241 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt 9301 tttatcttgt gcaatgtaac atcagagatt tt //
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