| 质粒名称: | SFG.wtCNa_opt.IRES.eGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22489 |
| 存储实验室: | Martin Pule |
|
载体骨架基本信息 |
|
| 载体名称: | SFG |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7678 bp |
| 载体类型: | 逆转录病毒表达 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | codon optimised wild type Calcineurin A,CNa |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1566 bp |
| 基因突变: | Codon Optimised wtCNa |
| GenBank编号 | GQ463593 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI (未破坏) |
| 3' 克隆位点: | MluI (未破坏) |
| 5' 测序引物 | ttacacagtcctgctgaccacc |
| 3' 测序引物 | caagcggcttcggccagtaac |
|
文献引用方法 |
|
| 材料和方法部分: | SFG.wtCNa_opt.IRES.eGFP was a gift from Martin Pule (Addgene plasmid # 22489 ; http://n2t.net/addgene:22489 ; RRID:Addgene_22489) |
| 参考文献部分: | Generation of EBV-specific cytotoxic T-cells that are resistant to calcineurin inhibitors for the treatment of post-transplant lymphoproliferative disease. Brewin J, Mancao C, Straathof K, Karlsson H, Samarasinghe S, Amrolia PJ, Pule M. Blood. 2009 Sep 21. ():. 10.1182/blood-2009-07-228387 PubMed 19770360 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS SFG.wtCNa_opt.IRES.eGFP 9244 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS SFG.wtCNa_opt.IRES.eGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9244)
AUTHORS Brewin J, Mancao C, Straathof K, Karlsson H, Samarasinghe S, Amrolia
PJ, Pule M
TITLE Generation of EBV-specific cytotoxic T-cells that are resistant to
calcineurin inhibitors for the treatment of post-transplant
lymphoproliferative disease.
JOURNAL Blood. 2009 Sep 21. ():.
PUBMED 19770360
REFERENCE 2 (bases 1 to 9244)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9244
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 655..1012
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
primer_bind 1423..1445
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1461..1477
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 1503..1877
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 1815..1831
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
misc_feature 3513..4064
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3657..3674)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3884..3903
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4064..4783
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 4064..4780
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4109..4130)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4370..4389)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4717..4738
/label=EGFP-C
/note="EGFP, forward primer"
LTR 5049..5518
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(6217..6234)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6217..6233)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6226..6248)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(6442..6461)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6561..6583
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6622..6640)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6708..6812
/gene="bla"
/label=AmpR promoter
CDS 6813..7673
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7031..7050)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7844..8432
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8333..8352
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8586..8603
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8720..8741
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8756..8786
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8794..8810
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8799..8821
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8818..8834
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8818..8834
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
LTR 9243..592
/note="long terminal repeat from Moloney murine leukemia
virus"
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttatgctccc cgagctcaat
421 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
781 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
841 tggcccgacc tgagtcctaa aatcccgatc gtttaggact ctttggtgca ccccccttag
901 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
961 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
1021 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggct
1081 agcctgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
1141 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
1201 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
1261 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtggggtaca
1321 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
1381 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
1441 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
1501 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
1561 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
1621 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
1681 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
1741 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
1801 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
1861 gaccatcctc tagactgcca ccatgagcga gcccaaggcc atcgacccca agctgtccac
1921 caccgacaga gtggtgaagg ccgtgccctt tcccccctcc caccgcctga ccgccaagga
1981 ggtgttcgac aacgatggca agcccagagt ggacatcctg aaagcccacc tgatgaagga
2041 gggcagactg gaggagagcg tggccctgcg catcatcacc gagggcgcca gcatcctgcg
2101 ccaggagaag aacctgctgg atatcgacgc ccctgtgacc gtgtgcggcg acatccacgg
2161 acagtttttc gacctgatga agctgttcga ggtgggcggc tctcccgcca acaccagata
2221 cctgttcctg ggcgactacg tggacagagg ctacttctct atcgagtgcg tgctgtacct
2281 gtgggccctg aagatcctgt accccaagac cctgttcctg ctgagaggca accacgagtg
2341 ccgccacctg accgagtact ttaccttcaa gcaggagtgc aagatcaagt acagcgagcg
2401 cgtgtacgat gcctgcatgg acgccttcga ctgtctgccc ctggccgctc tgatgaacca
2461 gcagttcctg tgtgtgcacg gaggcctgag ccccgagatc aacaccctgg acgacatccg
2521 gaagctggac cgcttcaagg agccccctgc ctatggcccc atgtgcgaca tcctgtggag
2581 cgaccccctg gaggactttg gcaacgagaa gacccaggag cactttaccc acaacaccgt
2641 gagaggctgc agctacttct actcctaccc tgctgtgtgc gagttcctgc agcacaacaa
2701 cctgctgagc atcctgaggg cccacgaggc ccaggatgcc ggctaccgca tgtaccgcaa
2761 gtcccagacc accggcttcc cctccctgat caccatcttc tctgccccca actacctgga
2821 cgtgtacaac aacaaagccg ctgtgctgaa gtacgagaac aacgtgatga acatccggca
2881 gttcaactgc tccccccacc cctactggct gcccaacttc atggacgtgt tcacctggtc
2941 cctgcccttt gtgggcgaga aggtgaccga gatgctggtg aacgtgctga acatctgctc
3001 cgacgatgag ctgggcagcg aggaggacgg cttcgatggc gccaccgctg ccgccagaaa
3061 ggaggtgatc cgcaataaga tcagagccat cggcaagatg gccagagtgt tctccgtgct
3121 gcgcgaggag agcgagtccg tgctgaccct gaagggcctg acccccaccg gcatgctgcc
3181 ctccggcgtg ctgagcggag gcaagcagac cctgcagagc gccacagtgg aggccatcga
3241 ggccgatgag gccatcaagg gcttctcccc ccagcacaag atcaccagct tcgaggaggc
3301 caagggcctg gaccggatca acgagcggat gccacccaga cgggatgcca tgcccagcga
3361 tgccaacctg aactccatca acaaggccct gaccagcgag accaacggca ccgacagcaa
3421 cggcagcaac tcctccaaca tccagtgaac gcgtacgtcg cgaccgcgga catgtacaga
3481 gctcgagcgg gatcaattcc gccccccccc taacgttact ggccgaagcc gcttggaata
3541 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
3601 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
3661 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
3721 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
3781 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
3841 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
3901 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
3961 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
4021 aaccacgggg acgtggtttt cctttgaaaa acacgataat aatatggtga gcaagggcga
4081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
4141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
4201 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
4261 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
4321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
4381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
4441 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
4501 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
4561 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
4621 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
4681 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
4741 cgccgccggg atcactctcg gcatggacga gctgtacaag taatccggat tagtccaatt
4801 tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct
4861 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa
4921 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
4981 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
5041 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
5101 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
5161 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
5221 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
5281 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
5341 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
5401 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
5461 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
5521 catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc
5581 atagtactta aagttacatt ggcttccttg aaataaacat ggagtattca gaatgtgtca
5641 taaatatttc taattttaag atagtatctc cattggcttt ctactttttc ttttattttt
5701 ttttgtcctc tgtcttccat ttgttgttgt tgttgtttgt ttgtttgttt gttggttggt
5761 tggttaattt ttttttaaag atcctacact atagttcaag ctagactatt agctactctg
5821 taacccaggg tgaccttgaa gtcatgggta gcctgctgtt ttagccttcc cacatctaag
5881 attacaggta tgagctatca tttttggtat attgattgat tgattgattg atgtgtgtgt
5941 gtgtgattgt gtttgtgtgt gtgactgtga aaatgtgtgt atgggtgtgt gtgaatgtgt
6001 gtatgtatgt gtgtgtgtga gtgtgtgtgt gtgtgtgtgc atgtgtgtgt gtgtgactgt
6061 gtctatgtgt atgactgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
6121 gttgtgaaaa aatattctat ggtagtgaga gccaacgctc cggctcaggt gtcaggttgg
6181 tttttgagac agagtctttc acttagcttg gaattcactg gccgtcgttt tacaacgtcg
6241 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
6301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
6361 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
6421 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
6481 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
6541 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
6601 gaaacgcgcg atgacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
6661 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
6721 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
6781 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
6841 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
6901 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
6961 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
7021 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
7081 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
7141 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
7201 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
7261 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
7321 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
7381 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
7441 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
7501 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
7561 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
7621 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
7681 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
7741 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
7801 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
7861 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
7921 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
7981 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
8041 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
8101 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
8161 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
8221 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
8281 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
8341 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
8401 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
8461 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
8521 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
8581 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
8641 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
8701 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
8761 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
8821 gaaacagcta tgaccatgat tacgccaagc tttgctctta ggagtttcct aatacatccc
8881 aaactcaaat atataaagca tttgacttgt tctatgccct agggggcggg gggaagctaa
8941 gccagctttt tttaacattt aaaatgttaa ttccatttta aatgcacaga tgtttttatt
9001 tcataagggt ttcaatgtgc atgaatgctg caatattcct gttaccaaag ctagtataaa
9061 taaaaataga taaacgtgga aattacttag agtttctgtc attaacgttt ccttcctcag
9121 ttgacaacat aaatgcgctg ctgagcaagc cagtttgcat ctgtcaggat caatttccca
9181 ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga catatacatg
9241 tgaa
//
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