载体名称: | pHC79-2cos/tk |
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来源历史: | <- W. Lindenmaier |
载体大小: | 11.7 kb |
质粒抗性: |
Ap抗性 in Escherichia coli, TK+ in animal cells
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载体说明: | 动物细胞和大肠杆菌的Cosmid克隆载体。pHC79-2cos/tk包含两个串联cos位点。可选择的标记是单纯疱疹病毒胸苷激酶基因(TK)和Apr基因。克隆地点:ClaI,HindIII。 |
宿主菌: | Escherichia coli K12 B1046 (derivative of strain HB101) |
培养条件: | LB培养基,37度 |
参考文献: |
Lindenmaier, W., Hauser, H., Greiser de Wilke, I., Schütz, G. (1982). Gene shuttling: moving of cloned DNA into and out of eukaryotic cells. Nucl.Acids Res. 10 : 1243-1256 . Lindenmaier, W., Hauser, H., Collins, J., (1985). An alternative gene cloning method for the isolation of human genes by expression in mouse cell clones. In 35. Colloquium - Mosbach 1984. The impact of gene transfer techniques in eucaryotic cell biology , pp. 17-25. Springer Verlag. |
安全等级: | 1级 |
分离国家: | 原产国未知 |
存储时间: | 1990年3月16日前 |