| 质粒名称: | pXPR_050 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 96925 |
| 存储实验室: | John Doench, David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pXPR |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 8343 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pXPR_050 was a gift from John Doench & David Root (Addgene plasmid # 96925 ; http://n2t.net/addgene:96925 ; RRID:Addgene_96925) |
| 参考文献部分: | Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG. Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8. 10.1038/s41467-018-07901-8 PubMed 30575746 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pXPR_050 8343 bp ds-DNA circular SYN 15-JUN-2022
DEFINITION lentiviral expression of gRNA scaffold.
ACCESSION .
VERSION .
KEYWORDS pXPR_050
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8343)
AUTHORS Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME,
Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG
TITLE Optimized libraries for CRISPR-Cas9 genetic screens with multiple
modalities.
JOURNAL Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8.
PUBMED 30575746
REFERENCE 2 (bases 1 to 8343)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 15, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8343
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(12..31)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 133..254
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(144..163)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 198..217
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
LTR 332..565
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
misc_feature 637..1225
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS 743..754
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind 1152..1172
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(1241..1840)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1324..1344)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1821..1840
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
promoter complement(1853..3031)
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 1862..2800
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind complement(1885..1905)
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
misc_feature 3169..3286
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter complement(3468..3708)
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind complement(3518..3537)
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
primer_bind complement(3688..3708)
/label=hU6-F
/note="Human U6 promoter, forward primer"
CDS complement(3886..3930)
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 4115..4348
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4841..4966
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 5013..5193
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(5194..5420)
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
primer_bind complement(5448..5468)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(5448..5466)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5487..5503)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5487..5503)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5500..5522)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5511..5527
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5535..5565)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5580..5601
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5718..5735)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5889..6477)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5969..5988)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6648..7508)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7271..7290
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7509..7613)
/gene="bla"
/label=AmpR promoter
rep_origin complement(7639..8094)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7776..7797)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7988..8007
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8221..8243
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 8235..8252
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 8236..8252
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8262..8281
/label=T7
/note="T7 promoter, forward primer"
promoter 8262..8280
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 8301..93
/label=SV40 ori
/note="SV40 origin of replication"
ORIGIN
1 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
61 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggatagctag
121 agccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
181 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
241 gcaataaaca agttcctctc actctctgat attcatttct ttgcaagtta taaatactga
301 ataataagat gacatgaact actatacgta ctgctagaga ttttccacac tgactaaaag
361 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
421 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
481 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
541 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
601 acagctgcct tgtaagtcat tggtcttaaa gtcgacgcgg ggaggcggcc caaagggaga
661 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
721 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
781 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
841 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
901 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
961 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
1021 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
1081 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
1141 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
1201 aaattttgta atccagaggt tgattgtcga cttaacgcgt tcaggcaccg ggcttgcggg
1261 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
1321 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
1381 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
1441 ggtcgggcga gactccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
1501 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
1561 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
1621 ccacggcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
1681 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc
1741 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt
1801 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtggccgta cgtcacgaca
1861 cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc
1921 caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt
1981 cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta
2041 aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact
2101 ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg
2161 actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc
2221 cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc
2281 agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag
2341 actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc
2401 gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca
2461 gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc
2521 aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc
2581 agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag
2641 gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat
2701 caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg
2761 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc
2821 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct
2881 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc
2941 tctaggcacc ggatcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg
3001 tgcgctctgc ccactgacgg gcaccggagc caattcccac tcctttcaag acctagaagg
3061 tccattagct gcaaagattc ctctctgttt aaaactttat ccatctttgc acccgggccc
3121 cctgagccgg cgccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat
3181 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
3241 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
3301 tgccatttgt ctcaagatct agttacgcca agcttgaatt caaaaaagca ccgactcggt
3361 gccacttttt caagttgata acggactagc cttatttcaa cttgctacag catttctgct
3421 gtagctctga aacagagacg caaaaaaccg gtcgtctccg gtgtttcgtc ctttccacaa
3481 gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat
3541 tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt
3601 attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct ctctaacagc
3661 cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctc tgggtcccct
3721 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
3781 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
3841 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
3901 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
3961 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
4021 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
4081 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
4141 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
4201 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
4261 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
4321 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
4381 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
4441 atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg
4501 ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt
4561 ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct actgtattat
4621 ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt
4681 gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc
4741 tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa
4801 ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc
4861 tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc
4921 ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc
4981 ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa
5041 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
5101 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
5161 ccaggctcag atctggtcta accagagaga cccgtttatg tatcgagcta ggcacttaaa
5221 tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca gacccgtctg
5281 ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg
5341 ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa
5401 gagtattgca taagactaca ttaagcttgc agctccagct tttgttccct ttagtgaggg
5461 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
5521 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
5581 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
5641 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
5701 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
5761 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
5821 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
5881 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
5941 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
6001 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
6061 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
6121 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
6181 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
6241 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
6301 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
6361 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
6421 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
6481 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
6541 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
6601 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
6661 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
6721 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
6781 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
6841 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
6901 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
6961 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
7021 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
7081 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
7141 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
7201 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
7261 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
7321 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
7381 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
7441 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
7501 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
7561 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
7621 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
7681 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
7741 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
7801 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
7861 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
7921 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
7981 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
8041 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
8101 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
8161 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
8221 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
8281 gcgaattggg tacgtcccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
8341 aga
//
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