| 出品公司: | Agilent |
|---|---|
| 载体名称: | AbVec2.0-mIghg1 |
| 质粒类型: | ORF、哺乳细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 载体大小: | 5.7kb |
| 载体抗性: | AMP |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 哺乳细胞 |
| 备注: | 逆病毒包装载体AbVec2.0-mIghg1适合包装pFB、pCFB逆病毒载体。 |
| 产品目录号: | #51243 |
| 物种: | 小鼠 |
| 温度: | 37度 |
买家导航
AbVec2.0-mIghg1载体质粒基本信息
AbVec2.0-mIghg1质粒图谱载体图谱和AbVec2.0-mIghg1载体序列质粒序列多克隆位点信息
AbVec2.0-mIghg1质粒载体简介
AbVec2.0-mIghg1质粒序列载体序列
LOCUS AbVec2.0-mIghg1 5746 bp DNA circular SYN 10-SEP-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5746)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 10, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5746
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 106..127
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 142..172
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 204..220
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
enhancer 249..628
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 629..832
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1063..1081
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
regulatory 2281..2290
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
polyA_signal 2294..2428
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2497..2854
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2705..2840
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(2874..2890)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3103..3558
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3840..3944
/gene="bla"
/label=AmpR promoter
CDS 3945..4805
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4976..5564
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga
241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa
1201 ctgcaaccgg ttgactaact agctgtgatc aaaacgacac ccccaagcgt ctatccactg
1261 gcccctggat ctgctgccca aactaactcc atggtgaccc tgggatgcct ggtcaagggc
1321 tatttccctg agccagtgac agtgacctgg aactctggat ccctgtccag cggtgtgcac
1381 accttcccag ctgtcctgca gtctgacctc tacactctga gcagctcagt gactgtcccc
1441 tccagcacct ggcccagcca gaccgtcacc tgcaacgttg cccacccggc cagcagcacc
1501 aaggtggaca agaaaattgt gcccagggat tgtggttgta agccttgcat atgtacagtc
1561 ccagaagtat catctgtctt catcttcccc ccaaagccca aggatgtgct caccattact
1621 ctgactccta aggtcacgtg tgttgtggta gacatcagca aggatgatcc cgaggtccag
1681 ttcagctggt ttgtagatga tgtggaggtg cacacagctc agacgaaacc ccgggaggag
1741 cagatcaaca gcactttccg ttcagtcagt gaacttccca tcatgcacca ggactggctc
1801 aatggcaagg agttcaaatg cagggtcaac agtgcagctt tccctgcccc catcgagaaa
1861 accatctcca aaaccaaagg cagaccgaag gctccacagg tgtacaccat tccacctccc
1921 aaggagcaga tggccaagga taaagtcagt ctgacctgca tgataacaaa cttcttccct
1981 gaagacatta ctgtggagtg gcagtggaat gggcagccag cggagaacta caagaacact
2041 cagcccatca tggacacaga tggctcttac ttcgtctaca gcaagctcaa tgtgcagaag
2101 agcaactggg aggcaggaaa tactttcacc tgctctgtgt tacatgaggg cctgcacaac
2161 caccatactg agaagagcct ctcccactct cctggtaaat gatcccagtg tccttggagc
2221 cctctggtcc tacaggactc tgacacctac ctccacccct ccctgtgtaa ataaagcttg
2281 gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
2341 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
2401 catcaatgta tcttatcatg tctggatcgg gaattaattc ggcgcagcac catggcctga
2461 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
2521 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2581 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
2641 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2701 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2761 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
2821 aggaggcttt tttggaggcc taggcttttg caaaaagctg ttaacagctt ggcactggcc
2881 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
2941 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
3001 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
3061 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
3121 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
3181 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
3241 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
3301 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
3361 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
3421 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
3481 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
3541 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
3601 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
3661 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
3721 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
3781 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
3841 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
3901 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
3961 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
4021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
4081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
4141 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
4201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
4261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
4321 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
4381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
4441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
4501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
4561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
4621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
4681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
4741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
4801 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
4861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
4921 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
4981 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
5041 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
5101 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
5161 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
5221 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
5281 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
5341 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
5401 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
5461 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
5521 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
5581 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
5641 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
5701 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//
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