| 质粒名称: | EGFP-Rab7A Q67L |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 28049 |
| 存储实验室: | Qing Zhong |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5446bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rab7A |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 623 bp |
| 基因突变: | Q67L |
| 标签/融合蛋白: | 2xHA（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | |
| 3' 克隆位点: | |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | EGFP-Rab7A Q67L was a gift from Qing Zhong (Addgene plasmid # 28049) |
| 参考文献部分: | Rubicon controls endosome maturation as a Rab7 effector. Sun Q, Westphal W, Wong KN, Tan I, Zhong Q. Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43. 10.1073/pnas.1010554107 PubMed 20974968 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS EGFP-Rab7A Q67L Sequencing Result 6780 bp DNA SYN
DEFINITION EGFP-Rab7A Q67L Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6780
/organism="EGFP-Rab7A Q67L Sequencing Result"
/mol_type="other DNA"
promoter 143..172
/label="lac_promoter"
misc_feature 186..208
/label="M13_pUC_rev_primer"
promoter 207..225
/label="M13_reverse_primer"
misc_feature complement(281..300)
/label="EBV_rev_primer"
CDS 514..1050
/label="ORF frame 1"
CDS complement(565..1359)
/label="ORF frame 1"
gene complement(568..1347)
/label="NeoR/KanR"
/gene="NeoR/KanR"
promoter complement(1474..1742)
/label="SV40_promoter"
misc_feature complement(1494..1513)
/label="SV40pro_F_primer"
rep_origin complement(1498..1575)
/label="SV40_origin"
misc_feature 1545..1760
/label="SV40_enhancer"
misc_feature 1740..1760
/label="pBABE_3_primer"
rep_origin complement(1874..2180)
/label="f1_origin"
terminator complement(2243..2470)
/label="bGH_PA_terminator"
misc_feature 2456..2473
/label="BGH_rev_primer"
promoter 2492..2509
/label="Sp6_promoter"
misc_feature 2493..2510
/label="Sp6_primer"
CDS complement(2546..3949)
/label="ORF frame 3"
CDS complement(3159..4070)
/label="ORF frame 2"
misc_feature complement(3197..3223)
/label="HA"
gene complement(3233..3946)
/label="EGFP"
/gene="EGFP"
misc_feature complement(3275..3296)
/label="EGFP_C_primer"
misc_feature complement(3737..3766)
/label="Y66 (EGFP)"
misc_feature 3883..3904
/label="EGFP_N_primer"
promoter complement(3963..3981)
/label="T7_promoter"
misc_feature complement(4055..4075)
/label="CMV_fwd_primer"
misc_feature complement(4241..4528)
/label="CAG_enhancer"
promoter 4910..4938
/label="AmpR_promoter"
gene 4980..5840
/label="Ampicillin"
/gene="Ampicillin"
CDS 4980..5840
/label="ORF frame 3"
rep_origin 5995..6614
/label="pBR322_origin"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAGCTGGC
61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT
241 AGCTAGAGGT CGACGGTATA CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC
301 AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT
361 TGTAACCATT ATAAGCTGCA ATAAACAAGT TGGGGTGGGC GAAGAACTCC AGCATGAGAT
421 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT
481 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG
541 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT
601 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC
661 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC
721 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG
781 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC GCGCCTTGAG
841 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC
901 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC
961 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA
1021 TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA
1081 TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC
1141 CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCCTGC AGTTCATTCA GGGCACCGGA
1201 CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC
1261 ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC
1321 GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT
1381 CTCTTGATCA GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG
1441 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCAGAGG CGGCCTCGGC
1501 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG
1561 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG
1621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA
1681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA
1741 CCCTAACTGA CACACATTCC ACAGAATTAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA
1801 TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG
1861 ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC
1921 AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC
1981 TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC
2041 CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA
2101 GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC
2161 ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTGGG GATACCCCCT AGAGCCCCAG
2221 CTGGTTCTTT CCGCCTCAGA AGCCATAGAG CCCACCGCAT CCCCAGCATG CCTGCTATTG
2281 TCTTCCCAAT CCTCCCCCTT GCTGTCCTGC CCCACCCCAC CCCCCAGAAT AGAATGACAC
2341 CTACTCAGAC AATGCGATGC AATTTCCTCA TTTTATTAGG AAAGGACAGT GGGAGTGGCA
2401 CCTTCCAGGG TCAAGGAAGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
2461 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC
2521 TCTAGATGCA TGCTCGAGCG GCCGCTTAGC AACTGCAGCT TTCTGCCGAG GCCTTGGCCC
2581 GGTCATTCTT GTCCAGTTTG ATAGGTTCAG GAAATTCGTT GTACAGCTCC ACCTCCGTTT
2641 CCTGCTTAAG TGCATTCCGT GCAATCGTCT GGAACGCCTG CTCCACGTTG ATGGCCTCCT
2701 TGGCACTGGT CTCAAAGTAG GGAATGTTGT TTTTGCTGTA GCACCAGGCC TGTGCCCGCT
2761 TTGTGGCCAC TTGTCTGTTT TCGAGGTCAA TCTTGTTTCC CAACACAACA AATGGGAAGT
2821 TTTCAGGATC TCGGGGACTG GCCTGGATGA GAAACTCATC TCTCCAGCTA TCTAGGGTTT
2881 TGAATGTGTT GGGGGCAGTC ACATCAAATA CCAGAACGCA GCAGTCTGCA CCTCTGTAGA
2941 AGGCCACACC GAGAGACTGG AACCGTTCCA GTCCTGCTGT GTCCCATATC TGCATTGTGA
3001 CTAGCCTGTC ATCCACCATC ACCTCCTTGG TCAGAAAGTC AGCTCCTATT GTGGCTTTGT
3061 ACTGATTGCT GAATTTCTTA TTCACATACT GGTTCATGAG TGATGTCTTC CCGACTCCAG
3121 AATCTCCCAG GATGATAACC TTCAGCAACA CTTTCTTCCT AGAGGTACCA GCGTAATCCG
3181 GAACATCGTA TGGGTAAGCG TAATCCGGAA CATCGTATGG GTACATGGAT CCCTTGTACA
3241 GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT
3301 CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG
3361 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA
3421 CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT
3481 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
3541 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
3601 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
3661 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
3721 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG
3781 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
3841 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
3901 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATA AGCTTGGGTC
3961 TCCCTATAGT GAGTCGTATT AATTTCGATA AGCCAGTAAG CAGTGGGTTC TCTAGTTAGC
4021 CAGAGAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT
4081 GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC
4141 CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC
4201 CCATTGATGT ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG
4261 TACTGCCAAG TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT
4321 TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC
4381 AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC
4441 GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG
4501 CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCGGAACT CCATATATGG GCTATGAACT
4561 AATGACCCCG TAATTGATTA CTATTAATAA CTAGTCAATA ATCAATGTCA ACGCGTATAT
4621 CTGGCCCGTA CATCGCGAAG CAGCGCAAAA CGCCTAACCC TAAGCAGATT CTTCATGCAA
4681 TTGTCGGTCA AGCCTTGCCT TGTTGTAGCT TAAATTTTGC TCGCGCACTA CTCAGCGACC
4741 TCCAACACAC AAGCAGGGAG CAGATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA
4801 CTGAGAGTGC ACCATAGGGG ATCGGGAGAT CTCCCGATCC GTCGACGTCA GGTGGCACTT
4861 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
4921 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
4981 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
5041 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
5101 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
5161 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
5221 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
5281 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
5341 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
5401 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
5461 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
5521 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
5581 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
5641 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
5701 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
5761 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
5821 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
5881 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
5941 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
6001 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
6061 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
6121 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG
6181 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
6241 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
6301 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
6361 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
6421 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
6481 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
6541 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
6601 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
6661 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
6721 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
//
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