| 出品公司: | Promega |
|---|---|
| 载体名称: | pSP73 |
| 质粒类型: | 大肠杆菌克隆载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 2464bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillian） |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | P2221 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
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pSP73载体质粒基本信息
pSP73质粒图谱载体图谱和pSP73载体序列质粒序列多克隆位点信息
pSP73质粒载体简介
The pSP73 Vector offers a wide range of restriction sites, providing greater versatility
in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA
polymerase promoters and a unique multiple cloning region, which includes restriction sites
for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII,
PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation
of the multiple cloning region.
pSP73质粒序列载体序列
LOCUS pSP73 2464 bp DNA SYN
DEFINITION pSP73
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..2464
/organism="pSP73"
/mol_type="other DNA"
promoter complement(102..120)
/label="T7_promoter"
rep_origin complement(363..982)
/label="pBR322_origin"
gene complement(1137..1997)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1137..1997)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(2039..2067)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(2226..2248)
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 2448..1
/label="Sp6_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gaaccagatc tgatatcatc gatgaattcg agctcggtac ccggggatcc tctagagtcg
61 acctgcaggc atgcaagctt cagctgctcg aggccggtct ccctatagtg agtcgtatta
121 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
181 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
541 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
781 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
1021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
1081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
1141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
1201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
1261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
1321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
1381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
1441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
1501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
1561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
1621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
1681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
1741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
1801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
1861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
1921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
1981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
2041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
2101 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
2161 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
2221 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
2281 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
2341 aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
2401 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
2461 tata
//
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