| 出品公司: | Promega |
|---|---|
| 载体名称: | pSP64 |
| 质粒类型: | 酵母双杂交系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 2999bp |
| 5' 测序引物及序列: | Sp6 Fwd: ATTTAGGTGACACTATAG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillian） |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pSP64载体质粒基本信息
pSP64质粒图谱载体图谱和pSP64载体序列质粒序列多克隆位点信息
pSP64质粒载体简介
The pSP64 Poly(A) Vector can be used as a standard cloning vector and for in vitro transcription
from the SP6 promoter. The pSP64 Poly(A) Vector also can be used to generate poly(A)+ transcripts
in vitro. The vector has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites.
Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI (HindIII, PstI, SalI,
AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of the recombinant plasmid with EcoRI
allows the use of SP6 RNA polymerase in vitro to prepare RNA copies of the inserted sequences that
contain a synthetic 3′ "poly(A)" tail of 30 residues.
pSP64质粒序列载体序列
LOCUS pSP64 2999 bp DNA SYN
DEFINITION pSP64
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..2999
/organism="pSP64"
/mol_type="other DNA"
promoter complement(63..81)
/label="M13_reverse_primer"
misc_feature complement(80..102)
/label="M13_pUC_rev_primer"
promoter complement(116..145)
/label="lac_promoter"
gene complement(1228..2088)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1228..2088)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(2130..2158)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(2317..2339)
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
gene complement(2490..2741)
/label="tet (887 - 636)"
/gene="tet (887 - 636)"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 2673..2692
/label="pBRrevBam_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 2983..1
/label="Sp6_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
1021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
1081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
1141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
1201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
1261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
1321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
1381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
1441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
1501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
1561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
1621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
1681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
1741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
1801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
1861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
1921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
1981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
2041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
2161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
2281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
2401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
2461 gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
2521 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
2581 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
2641 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
2701 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
2761 gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga
2821 aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag
2881 ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg
2941 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata
//
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