P336-FBP_11

LOCUS       P336-FBP_11         20768 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  T-DNA encoding Fungal Bioluminescent Pathway Components, control 
            pathway, missing Luz.
ACCESSION   .
VERSION     .
KEYWORDS    P336-FBP_11
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20768)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 20768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..20768
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(5..22)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5..21)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..36)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    224..248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1549..2178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2607..3680
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3746..3940
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4176..4198)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4284..4424
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4339..4358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4439..4456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4610..5198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4690..4709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5285..6079)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6504..6528
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6606..6780
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6837..7634)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6948..6967)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7558..7577
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7697..8373)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8564..8585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8600..8630
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8643..8665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8662..8678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8662..8678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    9374..9429
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        10692..11037
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      12188..12388
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     terminator      14165..14872
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
     4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
     4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
     5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
     5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
     5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
     5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
     6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
     6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
     6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
     6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
     6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
     6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
     6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta
     6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
     7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
     7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
     8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
     8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
     8701 tacccgggga tcggcgcgcc ctatggagat atgactggta agtttttctt gccaatacga
     8761 attagaaaac atgtctttga agatgaactg tatttttttt ttttactttg ttgtcatttt
     8821 aatgtacttt cttatcagga ttaaatcttc tgtaatttag agtagttttt ttaacaagat
     8881 aattaacaaa cttagagtaa tgaaaattga gatgttcagt tttcactcat atttcacatt
     8941 ttggtgaaag agtgggtagt atgcaacgtt ctaagtatgt ttggactttg tatcatgttg
     9001 ttttgattct ttgacgacat gtctatttgg gaaacaccaa tgacgtgtac cttgagactg
     9061 atacgattca aagggataga aacacgtcag atttacaagt ggcacctctt caatggacaa
     9121 tgggtattcc aatatgctaa gatgctacga gatatctaat ttatctaaca caactcaatt
     9181 ccaaaccaaa aatctgatgc cagctcgaca agacaaaaaa tctaagctca aaaatgtcaa
     9241 caaccaatag aaatcaaggc attgacgata tcacgagata agcaaattaa atcttcaagt
     9301 tttgcaattc atatgtacgt tataaatacc caaaaacctc accgtaacct agctatccta
     9361 ctgtattttt acaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt
     9421 tacaattaca atggctccta tctcctccac ctggtccaga ttaatcaggt tcgtagctat
     9481 agaaacgtca ctcgtgcata taggcgaacc gattgatgca actatggacg tagggcttgc
     9541 tcgtcgagaa gggaaaacta ttcaggccta tgagattatc ggatctggca gcgccctgga
     9601 cctttctgca caggtgagta agaacgtgct cacagtacga gaattgttga tgccactttc
     9661 tagagaagag ataaagacgg tcagatgctt aggccttaac tatcctgtcc acgcaacaga
     9721 agccaatgtt gctgtaccga agtttccgaa cctgttctac aaaccagtca caagtctcat
     9781 cgggcctgat ggtcttatca cgatccctag tgtagtgcaa ccgcctaagg aacatcagtc
     9841 agattatgaa gctgaacttg tgattgtaat cggaaaggcc gccaagaacg tgtctgagga
     9901 cgaagccttg gattatgtct taggctatac agctgctaac gatattagtt tccgaaaaca
     9961 ccagctcgca gtctcacagt ggtcattctc aaaaggattt ggatctctcc ttctcactat
    10021 acgtatggcc cagacacata gtggaaacat aaataggttc tcacgtgatc aaatctttaa
    10081 tgttaagaaa acaatcagct ttttgtctca aggaactacc ctcgaacctg ggtctatcat
    10141 tttgactggc acccctgatg gggtcggctt cgtcaggaac ccgcccttat acttaaaaga
    10201 tggggatgaa gtgatgactt ggataggttc cggcattggt acgcttgcta acaccgttca
    10261 agaagagaaa acatgctttg caagcggtgg gcatgagtag gcttttatgt tggtgatatg
    10321 gtggtaaatg tagggattta gtttacaatt gcgtatgtct gtgttggata tctgtagtgc
    10381 tgttcttatg gcttagatct tgtaatttct cattacagta tcaatgaata gatatcagtt
    10441 tctagtgatg acattggttc gtcttttagc tgttgattaa tttttcttaa ttgattcatc
    10501 ctattgcaat tcttctgaat ttaaattgta tactgtgaaa ttaagaaaat tcttgaaatt
    10561 aatgagaatt tgagtaatag cgctgttaga gctctgacgg cgcgccctat ggaggtcaac
    10621 atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt ctcagaagat
    10681 caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct cggattccat
    10741 tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa
    10801 tgccatcatt gcgataaagg aaaggctatc attcaagatc tctctgccga cagtggtccc
    10861 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagaggttcc aaccacgtct
    10921 acaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac
    10981 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
    11041 tcgagtataa gagctcattt ttacaacaat taccaacaac aacaaacaac aaacaacatt
    11101 acaattacat ttacaattat cgatacaatg gtgcaggaca cttcaagtgc tagcacaagt
    11161 ccaattttaa ccagatggta tatagacact cgacctctca cggcaagtac cgccgcactt
    11221 ccgctgctgg aaacgctgca accagctgat cagatctccg tccaaaaata ttatcatctc
    11281 aaggacaagc acatgtcact tgcctccaac cttttgaagt atttattcgt tcatcgaaat
    11341 tgccgaatac catggagtag tattgtcata tcaagaacgc ccgacccgca cagaaggcct
    11401 tgctatatac ctccttcagg ttcacaagag gactccttca aagacggata tacgggaatc
    11461 aacgtagaat tcaacgttag tcaccaagcc agcatggtgg caatcgcagg tacggctttc
    11521 acacctaata gcggtggaga tagcaaactc aaaccggagg tcggaataga tataacatgt
    11581 gtaaatgaga ggcagggcag gaatggagaa gaaagatcct tggaatccct gaggcagtat
    11641 atagatatat tcagcgaagt attcagcacc gcagagatgg ctaatataag gcgacttgat
    11701 ggggtttcct caagttccct tagtgcagac agactcgtag attatggata ccgattattc
    11761 tacacctatt gggccttaaa agaggcttat atcaagatga caggtgaggc cttattggct
    11821 ccttggttga gagagctgga gttttccaac gtggtcgctc cagccgctgt cgccgaaagc
    11881 ggggactctg ctggagactt tggcgaaccg tacactggcg tgcgtacaac attgtataag
    11941 aacctcgtag aggacgtacg aattgaggta gctgctttgg gaggagatta tctgttcgca
    12001 acagccgcta gaggtggtgg tataggcgca tcctcacgtc ctggtggtgg cccagatggc
    12061 tctggaatta gatcccagga cccttggagg ccgttcaaga aactcgatat agagcgtgac
    12121 atacagccat gtgctacggg cgtttgtaat tgcctgtcct caggataggc ttgatccccc
    12181 gtcgacagct agctatatca tcaatttatg tattacacat aatatcgcac tcagtctttc
    12241 atctacggca atgtaccagc tgatataatc agttattgaa atatttctga atttaaactt
    12301 gcatcaataa atttatgttt ttgcttggac tataatacct gacttgttat tttatcaata
    12361 aatatttaaa ctatatttct ttcaagatcg ctgttagagc tcggacggcg cgccctatgg
    12421 actggcagac atactgtccc acaaatgaag atggaatctg taaaagaaaa cgcgtgaaat
    12481 aatgcgtctg acaaaggtta ggtcggctgc ctttaatcaa taccaaagtg gtccctacca
    12541 cgatggaaaa actgtgcagt cggtttggct ttttctgacg aacaaataag attcgtggcc
    12601 gacaggtggg ggtccaccat gtgaaggcat cttcagactc caataatgga gcaatgacgt
    12661 aagggcttac gaaataagta agggtagttt gggaaatgtc cactcacccg tcagtctata
    12721 aatacttagc ccctccctca ttgttaaggg agcaaaatct cagagagata gtcctagaga
    12781 gagaaagaga gcaagtagcc tagaagtagt caaggcggcg aagtattcag gcacgtggcc
    12841 aggaagaaga aaagccaaga cgacgaaaac aggtaagagc taagcttaat ggcatccttt
    12901 gaaaattcac tcagtgtctt aatagttggg gctggcttag gtggtttggc cgctgctata
    12961 gcactgcgaa ggcaaggaca tgtagtcaag atatacgata gttcttcatt caaggccgaa
    13021 ttgggagcag gattagctgt accacccaat actctccgtt ccctgcaaca actgggctgt
    13081 aatacagaaa acctgaacgg agtagacaac ctctgcttta cggccatggg atacgacggg
    13141 agtgtaggta tgatgaataa tatgaccgac taccgtgagg catacggcac ttcctggata
    13201 atggtacatc gagtggatct ccacaacgag ctgatgcgag tcgccttgga tccgggcggg
    13261 ttagggcctc ctgccactct tcaccttaac catcgtgtta cgttctgcga tgtggatgcc
    13321 tgtacagtca cgtttacgaa cgggacgaca cagtctgctg acttgattgt cggagctgat
    13381 ggtattaggt ctacgattcg aagattcgtt ctggaggagg acgtgactgt tccagctagc
    13441 ggaattgtcg gctttcgatg gttggtccag gccgatgctc tcgacccata tcccgaactg
    13501 gattggattg tcaaaaaacc acccttggga gccaggttga tttctactcc acagaacccg
    13561 cagagtggcg taggactggc cgatagacga acgatcatta tttacgcatg cagaggtggg
    13621 accatggtta atgtattagc cgtacacgac gacgaaagag atcaaaacac tgctgattgg
    13681 tccgttcccg catcaaagga tgatttattc agagtgtttc acgattacca tccaagattc
    13741 cgacgactct tagagttagc acaggacatt aatttatggc agatgagggt ggtcccggtc
    13801 ttgaaaaaat gggtgaacaa gagggtatgc ttgctgggtg atgccgccca tgcttcactg
    13861 cccacacttg gacaaggctt cgggatgggg ttggaggatg cagtcgcttt gggcacgttg
    13921 ttgcctaagg gaacgaccgc atcacagatc gagacaaggc tggccgttta cgaacagctg
    13981 cgaaaggatc gagctgagtt tgtcgccgca gagagctatg aggagcaata tgtcccagag
    14041 atgagagggc tgtaccttag gtccaaagaa ttgagagaca gagtgatggg ttatgatatt
    14101 aaggtagagt cagagaaggt tttggaaaca ctgttgcgat catccaactc agcttgagct
    14161 tgtcctgctt taatgagata tgcgagaagc ctatgatcgc atgatatttg ctttcaattc
    14221 tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg
    14281 gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt
    14341 tcaatttact gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat
    14401 tgtgctgaat aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg
    14461 ttttattatt acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact
    14521 gattacataa atcttattca aatttcaaaa gtgccccagg ggctagtatc tacgacacac
    14581 cgagcggcga actaataacg ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg
    14641 gtgagattcc ttgaagttga gtattggccg tccgctctac cgaaagttac gggcaccatt
    14701 caacccggtc cagcacggcg gccgggtaac cgacttgctg ccccgagaat tatgcagcat
    14761 ttttttggtg tatgtgggcc ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa
    14821 tcgttgggcg ggtccagggc gaattttgcg acaacatgtc gaggctcagc aggaccgctg
    14881 ttagagctcc cggggcgcgc cctatggagt ggcagacata ctgtcccaca aatgaagatg
    14941 gaatctgtaa aagaaaacgc gtgaaataat gcgtctgaca aaggttaggt cggctgcctt
    15001 taatcaatac caaagtggtc cctaccacga tggaaaaact gtgcagtcgg tttggctttt
    15061 tctgacgaac aaataagatt cgtggccgac aggtgggggt ccaccatgtg aaggcatctt
    15121 cagactccaa taatggagca atgacgtaag ggcttacgaa ataagtaagg gtagtttggg
    15181 aaatgtccac tcacccgtca gtctataaat acttagcccc tccctcattg ttaagggagc
    15241 aaaatctcag agagatagtc ctagagagag aaagagagca agtagcctag aagtagtcaa
    15301 ggcggcgaag tattcaggca cgtggccagg aagaagaaaa gccaagacga cgaaaacagg
    15361 taagagctaa gcttaatgaa tagtagtaaa aaccctccat ccactctgtt agatgttttt
    15421 ctggataccg ctaggaacct tgatacagca tccagaaatg tcctggagtg cggagaacac
    15481 aggtggagtt acagagagtt ggacacagtg tcatccgcat tggcccaaca ccttcgatac
    15541 actgtgggtc ttagccccac tgtagccgtg attagcgaga atcaccccta catacttgca
    15601 ttaatgctcg ccgtatggaa actcggcgga acgttcgcac cgatagatgt tcattcccca
    15661 gccgaacttg ttgctggaat gttgaatatt gtaagcccgt catgtcttgt cattcctagc
    15721 tctgatgtga caaatcagac attggcatgc gatcttaata taccggttgt tgcattccac
    15781 ccacatcaaa gtacaattcc cgaactcaac aagaagtatc tcacagattc tcagatcagt
    15841 cctgacctgc ccttccccga ccctaatcga cctgctttgt acttattcac gtcatctgca
    15901 acgtctaggt ccaatttgaa gtgcgtgcca cttacacaca cattcatatt gcgaaactcc
    15961 ttaagtaaac gagcatggtg caaaagaatg aggccagaga cagattttga tggtattagg
    16021 gtcctggggt gggccccctg gagccatgtc ctcgctcata tgcaggacat tggccctctt
    16081 acattattga atgccgggtg ctacgtattt gccacgaccc catctaccta cccgacggag
    16141 ctgaaggatg accgtgatgt tattagttgc gccgcaaatg ccgtaatgta caaaggtgtt
    16201 aaatccttcg catgcctccc attcgtgctt gggggactca aggctttatg tgagtcagag
    16261 cccagtgtta aggcacaact gcaagtggag gaacgagccc agcttttgaa gtcactccaa
    16321 cacatgaaaa tcctggaatg cgggggggca atgctcgaag tgagtgtggc aagctgggct
    16381 atagagaata ggattcctat atctattggg attggtatga cagagacagg cggcgctctt
    16441 ttcgctgggc cggtccaagc aattcaaacc ggctttagca gtgaagataa atttatcgag
    16501 gacgcaacct acttgctggt caaggacgat tacgagagcc acgcagagga ggacataaac
    16561 gagggcgagt tagtggtaaa gtccagaatg ttacctaggg gttatttggg ctataacgac
    16621 ccctctttct ctgttgacga tgctggttgg gttactttca aaactggaga tcgatattca
    16681 gtcactcctg acggcaaatt ctcctggctt ggtcgaaata ctgatttcat tcagatgacg
    16741 agtggtgaga ctctcgatcc ccgtccgatt gagtccctcc tttgtgagtc cagccttata
    16801 tcacgagcct gtgtcatcgg ggataagttc ctgaacggac cagccacagc tgtatgcgct
    16861 attatagaac tcgaaccaac gactgttgag aaaggccagg ctcacagcag ggatattgcc
    16921 cgaatctttg cccccatcaa tcgagatctt cctcccccgt tacgtatagc atggagtcac
    16981 gtacttgtac ttcagccaag cgagaaaata ccgatgacga aaaagggtac aatcttccgt
    17041 aagaaaatcg aacaggtttt cggttccgct ttaggaggca gtagcggcga caactcacaa
    17101 gcaaccactg acgcttcagt agttaggagg gatgagttga gcaacacggt caaacatatc
    17161 ataagcagag ttttgggagt ctccgacgat gaactccttt ggacactgag ctttgctgag
    17221 cttggcatga cgtccgctct tgctactaga atagcaaatg aattgaacga agttctggta
    17281 ggtgtgaacc tccccatcaa tgcatgctac attcatgtgg acctgccttc tttgtcaaat
    17341 gccgtgtatg caaagttagc acaccttaaa ctcccggata ggacgcccga gccaaggaag
    17401 gccccagttg aaaatcccgg tggaaaggaa atcgtaatag taggtcaggc ttttaggctt
    17461 ccggggagta taaatgatgt cgcttctttg agggacgctt ttttggctag acaagcatcc
    17521 agtattatta ctgaaatacc gccagataga tgggaccacg catcatttta tccaaaagac
    17581 atacgattta ataaagcagg gctcgtcgac atcgctaact acgaccacag ttttttcgga
    17641 ttgacagcca cggaggcact gtacctgtca ccaacgatgc gtttggcact tgaagtgtcc
    17701 tttgaagcct tggagaatgc taatataccc gtgtcccagt taaagggctc tcaaacagct
    17761 gtatatgtcg caaccaccga cgacgggttt gagacactgc tgaatgctga ggcaggatat
    17821 gatgcataca cacgattcta tggcactggt agggccgcct ctacggccag tggacgaatt
    17881 agttatttac tcgacgtaca cggcccttca attaccgtag acactgcttg ttcaggcggt
    17941 gctgtttgca tagaccaggc catagactac ctccaatcct ctagcgcagc tgataccgcc
    18001 ataatctgcg ccagcaacac tcactgctgg ccaggttcct tcatgtttct gagtgctcaa
    18061 ggtatggtaa gttccggggg gcgatgtgca acttttacga cggatgccga cggctatgta
    18121 ccctctgaag gtgctgtggc atttatcctc aaaacccgag aagcagctat gagagataaa
    18181 gatactatct tggcaactat caaagcaacg cagattagtc ataatggtcg ttcccaaggt
    18241 ttagtcgcac caaacgtcaa ttcacaagct gatttacaca gaagcttatt gcagaaagct
    18301 ggtttgagtc cagccgacat tcacttcatc gaagctcacg ggacaggaac gtcactcggt
    18361 gaccttagtg aaatacaagc aattaatgat gcctacacgt catctcagcc tcgaacagcc
    18421 ggacccttaa tagtaagcgc aagtaaaacc gtcattggtc acacagagcc tgctggaccg
    18481 ttggtaggca tgctctcagt tctcaactcc tttaaagagg gcgcagttcc cggcttagct
    18541 cacctgacag cagataacct caatccggct ttagactgct cctccgtccc tttacttatc
    18601 ccctaccagc ctgtccactt ggcagctcct aagccacaca gggctgctgt gatgagttat
    18661 ggcttctccg gcactcttgg aggtatagtt cttgaagcac cggatgagga aaggcttgag
    18721 gaagaaccac ccaacgataa gcctatgtta tttgttgtta gcgccaaaac tcatactgca
    18781 ttgattgagt accttggacg ataccttgaa tttctgctcc aggccaatcc ccaagacttc
    18841 tgcgatatct gttacactag ctgcgttgga cgtgagcatt acaggtaccg ttttgcatgt
    18901 gtggcaaacg atatggaaga tttaattggc caacttcaga agaggcttgg gtctaaggtt
    18961 ccacctaaac cgtcctataa aagaggagcc ctcgcttttg ctttcagtgg acaagggacc
    19021 cagtttcgtg gaatggcaac agaattggct aaggcatata gtggttttcg taagatagtc
    19081 agtgatcttg ctaaaagagc atcagaactg tccggccatg caatcgacag gtttctgctt
    19141 gcatatgaca ttggggctga gaatgttgca ccggatagtg aagctgatca aatctgcata
    19201 ttcgtttacc aatgtagtgt tctcagatgg ctccaaacaa tggggattcg tccgtcagcc
    19261 gtgatcggtc acagcctcgg tgagattagc gcatccgtag ctgctggagc attgtccttg
    19321 gactctgccc ttgacctcgt aataagtaga gccaggttac tgaggtcatc tacgaatgcc
    19381 ccagctggca tggcagctat gtctgcatcc caggatgaag tagtcgaact cataggaaag
    19441 ctcgatttag ataaagctaa tagcctcagc gtgtccgtaa taaacggccc ccagaacacc
    19501 gttgtttcag gatcttctgc agcaatagaa agtatcgttg ctctcgcaaa gggacgaaaa
    19561 atcaaggcca gcgcactcaa cattaaccag gcattccata gtccttacgt cgattctgca
    19621 gtacccggct tacgagcttg gagtgagaaa catatatcat cagctcgacc attgcaaatc
    19681 cctttgtact ctaccctctt gggcgctcaa gtttctgagg gtcaaatgtt aaatcccgat
    19741 cactgggtag accatgctag gaaacccgtt cagttcgcac aagcagccac tataatgaag
    19801 gaatctttca ccggagtaat aatcgatatt ggacctcaag ttgtagcatg gtccctgctc
    19861 ttaagcaatg gtcttacttc cgtgaccgca ctcgccgcaa agcgtggccg ttcacagcaa
    19921 gtggccttcc tctccgcctt agctgatctt tatcaggact acggcgtcgt accagacttt
    19981 gttggactgt acgcacaaca ggaggacgct tccagactga aaaagaccga tatcttaact
    20041 tacccctttc agagggttcg acgttaccct tcctttatac cctctagaag ggcacccacc
    20101 cacgctcatg tccaagacga ggaaactctg tcatcaggat catcaactcc aaccttagaa
    20161 aatactgacc tggacagtgg gaaggaatct ctgatgggac caacaagagg gcttttgcga
    20221 gtcgatgatc ttagggactc aatagtcagt tcagtcaaag atgtcctgga attgaagagt
    20281 aacgaagatc tggacctcag cgagtccctt aatgctcttg gcatggactc tataatgttc
    20341 gctcaattac gaaaaaggat aggggagggc ctcggcctgt ccgttccaat ggtttttctc
    20401 agcgatgcct tcagtattgg agaaatggtc agtaacctcg tggaacaggc cgaagcttcc
    20461 gaggacaatt gagcttatat gaagatgaag atgaaatatt tggtgtgtca aataaaaagc
    20521 ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg ttatgaattt gtggcttttt
    20581 ctaatattaa atgaatgtaa gatcacatta taatgaataa acaaatgttt ctataatcca
    20641 ttgtgaatgt tttgttggat ctcttctgca gcatataact actgtatgtg ctatggtatg
    20701 gactatggaa tatgattaaa gataagcgct gttagagctc tcccggcgcg ccgatatcga
    20761 gctcagtg
//