出品公司: | Agilent |
---|---|
载体名称: | pNTAP-Shuttle-A |
质粒类型: | 腺病毒载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 7735 bp |
5' 测序引物及序列: | CMV Forward: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | DD tag (N-端) |
载体抗性: | 卡那霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | DH5α |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: | 腺病毒载体pNTAP-Shuttle-A载体表达表达N端融合链霉亲和素肽和钙结合肽的目的蛋白。 |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 腺病毒 |
买家导航
pNTAP-Shuttle-A载体质粒基本信息
pNTAP-Shuttle-A质粒图谱载体图谱和pNTAP-Shuttle-A载体序列质粒序列多克隆位点信息
pNTAP-Shuttle-A质粒载体简介
pNTAP-Shuttle-A载体限制性酶切位点
pNTAP Shuttle A, 7735 bp version 016001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AarI 1 3178
AatII 4 459, 512, 595, 781
Acc65I 1 1039
AccI 3 1201, 2815, 3753
AccIII 2 3908, 4135
AclI 1 6114
AcuI 6 1791, 1988, 3394, 5523, 6730
7162
AflIII 4 143, 1235, 3948, 4996
AhdI 2 2114, 5884
Alw44I 2 2498, 5310
AlwNI 5 1794, 1952, 2037, 5407, 6224
ApaI 2 2608, 2914
ApoI 7 242, 966, 1433, 2509, 3781
7270, 7281
AseI 3 4278, 6060, 7720
AvaI 6 1181, 1194, 1214, 1929, 2109
4213
AvaII 7 2113, 2137, 3162, 3303, 3381
6027, 7121
BamHI 2 1188, 4813
BanII 9 1153, 1630, 1687, 1843, 2608
2914, 3633, 6966, 7539
BbeI 4 1177, 3507, 6356, 6604
BbsI 2 1053, 3408
BciVI 3 2491, 5205, 6813
BclI 2 1276, 6446
BfrBI 3 2404, 2582, 4807
BglI 7 422, 544, 615, 1091, 1985
4063, 6003
BglII 1 934
BlnI 1 4323
Bme1580I 7 1019, 2498, 2608, 2914, 5310
6547, 6640
BmgBI 3 65, 2158, 4728
BmrI 5 632, 5934, 6229, 6310, 6544
BmtI 1 940
BpmI 5 1105, 1126, 1690, 4347, 5974
Bpu10I 3 1840, 2884, 2980
BpuEI 6 3372, 3762, 5102, 5364, 5641
6996
BsaAI 7 676, 1078, 2388, 4630, 6176
6907, 7466
BsaBI 4 1277, 2050, 4175, 6460
BsaHI 10 459, 512, 595, 781, 1177
1198, 2554, 3507, 6356, 6604
BsaI 5 1745, 2121, 3109, 3609, 5956
BsaWI 8 1260, 2494, 3908, 4135, 5202
5349, 6338, 6636
BseMII 9 981, 1139, 1841, 1950, 2885
2981, 5271, 5680, 5846
BseRI 3 3210, 3502, 4122
BseYI 9 1173, 2286, 2373, 2440, 2865
3062, 5300, 6351, 7109
BsgI 4 1860, 2470, 3525, 4481
BsiEI 7 1208, 1242, 2126, 4201, 4909
5333, 6511
BsiHKAI 9 1129, 1600, 1630, 2498, 3258
3633, 5310, 6714, 6904
BsmBI 3 978, 2205, 2552
BsmFI 8 499, 650, 818, 2112, 3010
3163, 3747, 6742
BsmI 4 1364, 1463, 2584, 3615
BspCNI 9 981, 1139, 1841, 1950, 2885
2981, 5271, 5680, 5846
BspHI 1 5716
BspMI 8 1975, 2819, 2877, 3179, 3898
6272, 6501, 6864
BsrBI 5 1211, 4344, 4927, 7216, 7610
BsrDI 4 2603, 5943, 6125, 6831
BsrGI 1 192
BssHII 5 2095, 2097, 3353, 3484, 7669
BssSI 4 3514, 3621, 5169, 7197
Bst1107I 1 3753
BstAPI 2 1853, 3072
BstEII 2 3175, 4091
BstXI 1 2592
Bsu36I 1 1138
BtgI 9 698, 1043, 1658, 1813, 2612
3291, 3443, 3467, 7037
BtgZI 7 687, 1884, 2828, 6891, 7042
7235, 7454
BtsI 5 1459, 1857, 2228, 3522, 6283
ClaI 1 1229
Csp6I 10 193, 556, 581, 636, 669
720, 877, 1040, 3243, 6910
DdeI 10 981, 1139, 1841, 1950, 2524
2885, 2981, 5271, 5680, 5846
DraI 6 1319, 2016, 3774, 3998, 5753
5772
DraIII 2 1078, 7463
DrdI 4 3441, 5098, 6626, 7417
EagI 2 1208, 6511
EarI 3 4874, 6954, 7164
Eco47III 3 2283, 3398, 4363
EcoICRI 2 1630, 3633
EcoO109I 5 1088, 2136, 2339, 3302, 3596
EcoRI 1 3781
EcoRV 1 1248
FspI 2 6109, 6705
HgaI 10 855, 1199, 1204, 1237, 1672
2554, 3421, 5098, 5676, 7695
HincII 3 1201, 1380, 1894
HindIII 1 1223
HpaI 1 1380
KasI 4 1177, 3507, 6356, 6604
KpnI 1 1039
MluI 1 1235
MmeI 5 1765, 1960, 5186, 5370, 7420
MscI 3 268, 3359, 6685
MslI 5 699, 2593, 2658, 3879, 7038
MunI 2 1369, 1913
NaeI 4 1096, 4068, 7105, 7569
NarI 4 1177, 3507, 6356, 6604
NcoI 5 698, 1043, 1658, 3443, 7037
NdeI 2 571, 4818
NgoMIV 4 1096, 4068, 7105, 7569
NheI 1 940
NotI 1 1207
NsiI 3 2404, 2582, 4807
NspI 9 143, 1650, 3006, 3021, 3126
3948, 4805, 4996, 7006
PacI 2 4798, 7728
PciI 3 143, 3948, 4996
PfoI 2 2153, 2669
PmeI 1 3773
PmlI 1 1078
PpuMI 2 2136, 3302
PsiI 3 1400, 4040, 7338
PspOMI 2 2608, 2914
PstI 5 1773, 1800, 2230, 2963, 6130
PvuI 1 1242
PvuII 7 1111, 1955, 2863, 2890, 2966
6349, 6709
RsaI 10 193, 556, 581, 636, 669
720, 877, 1040, 3243, 6910
RsrII 1 7120
SacI 2 1630, 3633
SacII 2 1813, 3467
SalI 1 1201
SapI 3 4873, 6954, 7164
SexAI 1 3658
SfcI 8 1773, 1800, 2230, 2963, 5261
5452, 6130, 7686
SfoI 4 1177, 3507, 6356, 6604
SgrAI 1 186
SmaI 5 1181, 1194, 1929, 2109, 4213
SmlI 7 1214, 3372, 3762, 5102, 5364
5641, 6996
SnaBI 2 676, 4630
SphI 3 1650, 3126, 7006
SrfI 1 1180
StyI 10 698, 1043, 1658, 2343, 3443
3479, 3497, 4323, 6414, 7037
TatI 5 192, 555, 635, 668, 719
TfiI 7 1919, 2460, 3568, 4175, 4971
7090, 7224
TspGWI 4 154, 3742, 4053, 4615
Tth111I 2 1747, 6720
Van91I 3 1058, 1077, 2154
XbaI 1 1266
XcmI 3 2036, 3627, 6414
XhoI 1 1214
XmaI 5 1181, 1194, 1929, 2109, 4213
XmnI 1 7718
ZraI 4 459, 512, 595, 781
Enzymes that do NOT cut molecule:
AflII AgeI AleI AscI AsiSI
BbvCI BlpI BsiWI BstBI EcoNI
FseI FspAI NruI PshAI SanDI
SbfI ScaI SfiI SpeI SspI
StuI SwaI
pNTAP-Shuttle-A质粒序列载体序列
pNTAP Shuttle A, 7735 bp version 016001 NOTE: The following sequence has been verified for accuracy at the junctions. The remainder of the sequence has been obtained from existing data. 1 CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG 51 GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG 101 TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA 151 GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG 201 GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG 251 CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA 301 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA 351 GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 401 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC 451 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 501 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT 551 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC 601 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG 651 GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 701 TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC 751 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 801 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC 851 CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC 901 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CTAGCATGAA 951 GCGACGATGG AAAAAGAATT TCATAGCCGT CTCAGCAGCC AACCGCTTTA 1001 AGAAAATCTC ATCCTCCGGG GCACTTGGAA GCGGTAGCGG TACCATGGAC 1051 GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG 1101 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC 1151 GGGAGCCCTC CGGCGGCTGC AAGCTGGGCG CCCGGGCGGA TCCCCCGGGC 1201 GTCGACGCGG CCGCTCGAGC CGAAGCTTAT CGATACGCGT CCGATCGGAT 1251 ATCCGATCCA CCGGATCTAG ATAACTGATC ATAATCAGCC ATACCACATT 1301 TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC 1351 TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT 1401 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 1451 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 1501 CTTAACGCGG ATCTGGGCGT GGTTAAGGGT GGGAAAGAAT ATATAAGGTG 1551 GGGGTCTTAT GTAGTTTTGT ATCTGTTTTG CAGCAGCCGC CGCCGCCATG 1601 AGCACCAACT CGTTTGATGG AAGCATTGTG AGCTCATATT TGACAACGCG 1651 CATGCCCCCA TGGGCCGGGG TGCGTCAGAA TGTGATGGGC TCCAGCATTG 1701 ATGGTCGCCC CGTCCTGCCC GCAAACTCTA CTACCTTGAC CTACGAGACC 1751 GTGTCTGGAA CGCCGTTGGA GACTGCAGCC TCCGCCGCCG CTTCAGCCGC 1801 TGCAGCCACC GCCCGCGGGA TTGTGACTGA CTTTGCTTTC CTGAGCCCGC 1851 TTGCAAGCAG TGCAGCTTCC CGTTCATCCG CCCGCGATGA CAAGTTGACG 1901 GCTCTTTTGG CACAATTGGA TTCTTTGACC CGGGAACTTA ATGTCGTTTC 1951 TCAGCAGCTG TTGGATCTGC GCCAGCAGGT TTCTGCCCTG AAGGCTTCCT 2001 CCCCTCCCAA TGCGGTTTAA AACATAAATA AAAAACCAGA CTCTGTTTGG 2051 ATTTGGATCA AGCAAGTGTC TTGCTGTCTT TATTTAGGGG TTTTGCGCGC 2101 GCGGTAGGCC CGGGACCAGC GGTCTCGGTC GTTGAGGGTC CTGTGTATTT 2151 TTTCCAGGAC GTGGTAAAGG TGACTCTGGA TGTTCAGATA CATGGGCATA 2201 AGCCCGTCTC TGGGGTGGAG GTAGCACCAC TGCAGAGCTT CATGCTGCGG 2251 GGTGGTGTTG TAGATGATCC AGTCGTAGCA GGAGCGCTGG GCGTGGTGCC 2301 TAAAAATGTC TTTCAGTAGC AAGCTGATTG CCAGGGGCAG GCCCTTGGTG 2351 TAAGTGTTTA CAAAGCGGTT AAGCTGGGAT GGGTGCATAC GTGGGGATAT 2401 GAGATGCATC TTGGACTGTA TTTTTAGGTT GGCTATGTTC CCAGCCATAT 2451 CCCTCCGGGG ATTCATGTTG TGCAGAACCA CCAGCACAGT GTATCCGGTG 2501 CACTTGGGAA ATTTGTCATG TAGCTTAGAA GGAAATGCGT GGAAGAACTT 2551 GGAGACGCCC TTGTGACCTC CAAGATTTTC CATGCATTCG TCCATAATGA 2601 TGGCAATGGG CCCACGGGCG GCGGCCTGGG CGAAGATATT TCTGGGATCA 2651 CTAACGTCAT AGTTGTGTTC CAGGATGAGA TCGTCATAGG CCATTTTTAC 2701 AAAGCGCGGG CGGAGGGTGC CAGACTGCGG TATAATGGTT CCATCCGGCC 2751 CAGGGGCGTA GTTACCCTCA CAGATTTGCA TTTCCCACGC TTTGAGTTCA 2801 GATGGGGGGA TCATGTCTAC CTGCGGGGCG ATGAAGAAAA CGGTTTCCGG 2851 GGTAGGGGAG ATCAGCTGGG AAGAAAGCAG GTTCCTGAGC AGCTGCGACT 2901 TACCGCAGCC GGTGGGCCCG TAAATCACAC CTATTACCGG GTGCAACTGG 2951 TAGTTAAGAG AGCTGCAGCT GCCGTCATCC CTGAGCAGGG GGGCCACTTC 3001 GTTAAGCATG TCCCTGACTC GCATGTTTTC CCTGACCAAA TCCGCCAGAA 3051 GGCGCTCGCC GCCCAGCGAT AGCAGTTCTT GCAAGGAAGC AAAGTTTTTC 3101 AACGGTTTGA GACCGTCCGC CGTAGGCATG CTTTTGAGCG TTTGACCAAG 3151 CAGTTCCAGG CGGTCCCACA GCTCGGTCAC CTGCTCTACG GCATCTCGAT 3201 CCAGCATATC TCCTCGTTTC GCGGGTTGGG GCGGCTTTCG CTGTACGGCA 3251 GTAGTCGGTG CTCGTCCAGA CGGGCCAGGG TCATGTCTTT CCACGGGCGC 3301 AGGGTCCTCG TCAGCGTAGT CTGGGTCACG GTGAAGGGGT GCGCTCCGGG 3351 CTGCGCGCTG GCCAGGGTGC GCTTGAGGCT GGTCCTGCTG GTGCTGAAGC 3401 GCTGCCGGTC TTCGCCCTGC GCGTCGGCCA GGTAGCATTT GACCATGGTG 3451 TCATAGTCCA GCCCCTCCGC GGCGTGGCCC TTGGCGCGCA GCTTGCCCTT 3501 GGAGGAGGCG CCGCACGAGG GGCAGTGCAG ACTTTTGAGG GCGTAGAGCT 3551 TGGGCGCGAG AAATACCGAT TCCGGGGAGT AGGCATCCGC GCCGCAGGCC 3601 CCGCAGACGG TCTCGCATTC CACGAGCCAG GTGAGCTCTG GCCGTTCGGG 3651 GTCAAAAACC AGGTTTCCCC CATGCTTTTT GATGCGTTTC TTACCTCTGG 3701 TTTCCATGAG CCGGTGTCCA CGCTCGGTGA CGAAAAGGCT GTCCGTGTCC 3751 CCGTATACAG ACTTGAGAGG GAGTTTAAAC GAATTCAATA GCTTGTTGCA 3801 TGGGCGGCGA TATAAAATGC AAGGTGCTGC TCAAAAAATC AGGCAAAGCC 3851 TCGCGCAAAA AAGAAAGCAC ATCGTAGTCA TGCTCATGCA GATAAAGGCA 3901 GGTAAGCTCC GGAACCACCA CAGAAAAAGA CACCATTTTT CTCTCAAACA 3951 TGTCTGCGGG TTTCTGCATA AACACAAAAT AAAATAACAA AAAAACATTT 4001 AAACATTAGA AGCCTGTCTT ACAACAGGAA AAACAACCCT TATAAGCATA 4051 AGACGGACTA CGGCCATGCC GGCGTGACCG TAAAAAAACT GGTCACCGTG 4101 ATTAAAAAGC ACCACCGACA GCTCCTCGGT CATGTCCGGA GTCATAATGT 4151 AAGACTCGGT AAACACATCA GGTTGATTCA CATCGGTCAG TGCTAAAAAG 4201 CGACCGAAAT AGCCCGGGGG AATACATACC CGCAGGCGTA GAGACAACAT 4251 TACAGCCCCC ATAGGAGGTA TAACAAAATT AATAGGAGAG AAAAACACAT 4301 AAACACCTGA AAAACCCTCC TGCCTAGGCA AAATAGCACC CTCCCGCTCC 4351 AGAACAACAT ACAGCGCTTC CACAGCGGCA GCCATAACAG TCAGCCTTAC 4401 CAGTAAAAAA GAAAACCTAT TAAAAAAACA CCACTCGACA CGGCACCAGC 4451 TCAATCAGTC ACAGTGTAAA AAAGGGCCAA GTGCAGAGCG AGTATATATA 4501 GGACTAAAAA ATGACGTAAC GGTTAAAGTC CACAAAAAAC ACCCAGAAAA 4551 CCGCACGCGA ACCTACGCCC AGAAACGAAA GCCAAAAAAC CCACAACTTC 4601 CTCAAATCGT CACTTCCGTT TTCCCACGTT ACGTAACTTC CCATTTTAAG 4651 AAAACTACAA TTCCCAACAC ATACAAGTTA CTCCGCCCTA AAACCTACGT 4701 CACCCGCCCC GTTCCCACGC CCCGCGCCAC GTCACAAACT CCACCCCCTC 4751 ATTATCATAT TGGCTTCAAT CCAAAATAAG GTATATTATT GATGATGTTA 4801 ATTAACATGC ATGGATCCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA 4851 AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 4901 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 4951 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 5001 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 5051 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 5101 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 5151 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 5201 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 5251 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 5301 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 5351 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 5401 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 5451 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 5501 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 5551 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 5601 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 5651 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 5701 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 5751 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 5801 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 5851 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 5901 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 5951 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 6001 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 6051 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 6101 TAATAGTTTG CGCAACGTTG TTGCCATTGC TGCAGCCATG AGATTATCAA 6151 AAAGGATCTT CACCTAGATC CTTTTCACGT AGAAAGCCAG TCCGCAGAAA 6201 CGGTGCTGAC CCCGGATGAA TGTCAGCTAC TGGGCTATCT GGACAAGGGA 6251 AAACGCAAGC GCAAAGAGAA AGCAGGTAGC TTGCAGTGGG CTTACATGGC 6301 GATAGCTAGA CTGGGCGGTT TTATGGACAG CAAGCGAACC GGAATTGCCA 6351 GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT 6401 GGCTTTCTTG CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGCTCTGATC 6451 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 6501 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 6551 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 6601 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 6651 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT 6701 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 6751 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 6801 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 6851 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 6901 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 6951 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA 7001 GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT 7051 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 7101 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 7151 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 7201 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 7251 CTTCTTGACG AGTTCTTCTG AATTTTGTTA AAATTTTTGT TAAATCAGCT 7301 CATTTTTTAA CCAATAGGCC GAAATCGGCA CCATCCCTTA TAAATCAAAA 7351 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC 7401 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC 7451 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGTGG 7501 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT 7551 TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA 7601 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG 7651 CGCGTAACCA CCACACCCGC GCGCTTAATG CGCCGCTACA GGGCGCGTCC 7701 ATTCGCCATT CAGGATCGAA TTAATTCTTA ATTAA
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pNTAP-Shuttle-A质粒载体应用举例
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