| 质粒名称: | pGH335_MS2-AID*Δ-Hygro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 85406 |
| 存储实验室: | Michael Bassik |
|
载体骨架基本信息 |
|
| 载体名称: | Addgene #61426 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MS2-AID*Δ and Hygromycin resistance |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | - |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pGH335_MS2-AID*Δ-Hygro was a gift from Michael Bassik (Addgene plasmid # 85406 ; http://n2t.net/addgene:85406 ; RRID:Addgene_85406) |
| 参考文献部分: | Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. 10.1038/nmeth.4038 PubMed 27798611 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGH335_MS2-AID*Delta-Hygro 11382 bp ds-DNA circular SYN 18-MAR-2020
DEFINITION lentiviral expression vector for MS2-AID*Delta fusion protein with
hygromycin B selectable marker.
ACCESSION .
VERSION .
KEYWORDS pGH335_MS2-AID*Delta-Hygro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11382)
AUTHORS Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
Bassik MC
TITLE Directed evolution using dCas9-targeted somatic hypermutation in
mammalian cells.
JOURNAL Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
PUBMED 27798611
REFERENCE 2 (bases 1 to 11382)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 18, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11382
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2687..3865
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2918..3856
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3813..3833
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 3872..3881
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3881..4267
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
CDS 4322..4342
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4958..5011
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 5015..6034
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
"
misc_feature 6074..6662
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6127..6147)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6545..6556)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6665..6681)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6666..6682)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 7187..7367
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7393..7410)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7399..7623
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7669..8097
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7756..7775)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7966..7987
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8106..8126)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8111..8440
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8291..8426
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8353..8372
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 8488..8535
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 8554..8928
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 9058..9179
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9095..9114)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9149..9168
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9228..9244)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9228..9244)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9241..9263)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9252..9268
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9276..9306)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9321..9342
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9459..9476)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9630..10218)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9710..9729)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10389..11249)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11012..11031
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11250..11354)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tgaaggacgc ggcgctcggg
3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gcttcaaact ttactcagtt
3901 cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta atttcgctaa
3961 tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg tgacatgcag
4021 cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg tccccaaagt
4081 ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa
4141 catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca tcgtgaaggc
4201 aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg ctaactcagg
4261 tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg
4321 acctaagaaa aagaggaagg tggcggccgc tggatccatg gacagcctct tgatgaaccg
4381 gagggagttt ctttaccaat tcaaaaatgt ccgctgggct aagggtcggc gtgagaccta
4441 cctgtgctac gtagtgaaga ggcgtgacag tgctacatcc ttttcactgg actttggtta
4501 tcttcgcaat aagaacggct gccacgtgga attgctcttc ctccgctaca tctcggactg
4561 ggacctagac cctggccgct gctaccgcgt cacctggttc atctcctgga gcccctgcta
4621 cgactgtgcc cgacatgtgg ccgactttct gcgagggaac cccaacctca gtctgaggat
4681 cttcaccgcg cgcctctact tctgtgagga ccgcaaggct gagcccgagg ggctgcggcg
4741 gctgcaccgc gccggggtgc aaatagccat catgaccttc aaagattatt tttactgctg
4801 gaatactttt gtagaaaacc acggaagaac tttcaaagcc tgggaagggc tgcatgaaaa
4861 ttcagttcgt ctctccagac agcttcggcg catccttttg cccctgtatg aggttgatga
4921 cttacgagac gcatttcgta cttgtacagg cagtggagag ggcagaggaa gtctgctaac
4981 atgcggtgac gtcgaggaga atcctggccc aaccatgaaa aagcctgaac tcaccgctac
5041 ctctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
5101 cgagggcgaa gaatctcggg ctttcagctt cgatgtggga gggcgtggat atgtcctgcg
5161 ggtgaatagc tgcgccgatg gtttctacaa agatcgctat gtttatcggc actttgcatc
5221 cgccgctctc cctattcccg aagtgcttga cattggggag ttcagcgaga gcctgaccta
5281 ttgcatctcc cgccgtgcac agggtgtcac cttgcaagac ctgcctgaaa ccgaactgcc
5341 cgctgttctc cagcccgtcg ccgaggccat ggatgccatc gctgccgccg atcttagcca
5401 gaccagcggg ttcggcccat tcggacctca aggaatcggt caatacacta catggcgcga
5461 tttcatctgc gctattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
5521 cgtcagtgcc tccgtcgccc aggctctcga tgagctgatg ctttgggccg aggactgccc
5581 cgaagtccgg cacctcgtgc acgccgattt cggctccaac aatgtcctga ccgacaatgg
5641 ccgcataaca gccgtcattg actggagcga ggccatgttc ggggattccc aatacgaggt
5701 cgccaacatc ttcttctgga ggccctggtt ggcttgtatg gagcagcaga cccgctactt
5761 cgagcggagg catcccgagc ttgcaggatc tcctcggctc cgggcttata tgctccgcat
5821 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
5881 tcagggtcgc tgcgacgcaa tcgtccggtc cggagccggg actgtcgggc gtacacaaat
5941 cgcccgcaga agcgctgccg tctggaccga tggctgtgtg gaagtgctcg ccgatagtgg
6001 aaacagacgc cccagcactc gtcctagggc aaaggatctg cagtaatgag aattcgatat
6061 caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
6121 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
6181 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
6241 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
6301 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
6361 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
6421 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
6481 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
6541 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
6601 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
6661 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
6721 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
6781 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
6841 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
6901 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
6961 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
7021 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
7081 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
7141 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
7201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
7261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
7321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
7381 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
7441 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
7501 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
7561 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
7621 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
7681 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
7741 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
7801 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
7861 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
7921 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
7981 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
8041 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
8101 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
8161 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
8221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
8281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
8341 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
8401 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
8461 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
8521 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
8581 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
8641 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
8701 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
8761 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
8821 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
8881 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
8941 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
9001 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
9061 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
9121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
9181 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
9241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
9301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
9361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
9421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
9481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
9541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
9601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
9661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
9721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
9781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
9841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
9901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
9961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
10021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
10081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
10141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
10201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
10261 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
10321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
10381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
10441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
10501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
10561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
10621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
10681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
10741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
10801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
10861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
10921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
10981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
11041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
11101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
11161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
11221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
11281 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
11341 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
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