出品公司: | Agilent |
---|---|
载体名称: | pNTAP-Shuttle-B |
质粒类型: | 腺病毒载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 7736 bp |
5' 测序引物及序列: | CMV Forward: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | SBP ; CBP (N-端) |
载体抗性: | 卡那霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | DH5α |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: | 腺病毒载体pNTAP-Shuttle-B载体表达表达N端融合链霉亲和素肽和钙结合肽的目的蛋白。 |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 腺病毒 |
买家导航
pNTAP-Shuttle-B载体质粒基本信息
pNTAP-Shuttle-B质粒图谱载体图谱和pNTAP-Shuttle-B载体序列质粒序列多克隆位点信息
pNTAP-Shuttle-B质粒载体简介
pNTAP-Shuttle-B限制性酶切位点
pNTAP Shuttle B, 7736 bp version 016001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AarI 1 3179
AatII 4 459, 512, 595, 781
Acc65I 1 1039
AccI 3 1202, 2816, 3754
AccIII 2 3909, 4136
AclI 1 6115
AcuI 6 1792, 1989, 3395, 5524, 6731
7163
AflIII 4 143, 1236, 3949, 4997
AhdI 2 2115, 5885
Alw44I 2 2499, 5311
AlwNI 5 1795, 1953, 2038, 5408, 6225
ApaI 2 2609, 2915
ApoI 7 242, 966, 1434, 2510, 3782
7271, 7282
AseI 3 4279, 6061, 7721
AvaI 6 1182, 1195, 1215, 1930, 2110
4214
AvaII 7 2114, 2138, 3163, 3304, 3382
6028, 7122
BamHI 2 1189, 4814
BanII 9 1153, 1631, 1688, 1844, 2609
2915, 3634, 6967, 7540
BbeI 3 3508, 6357, 6605
BbsI 2 1053, 3409
BciVI 3 2492, 5206, 6814
BclI 2 1277, 6447
BfrBI 3 2405, 2583, 4808
BglI 7 422, 544, 615, 1091, 1986
4064, 6004
BglII 1 934
BlnI 1 4324
Bme1580I 7 1019, 2499, 2609, 2915, 5311
6548, 6641
BmgBI 3 65, 2159, 4729
BmrI 5 632, 5935, 6230, 6311, 6545
BmtI 1 940
BpmI 5 1105, 1126, 1691, 4348, 5975
Bpu10I 3 1841, 2885, 2981
BpuEI 6 3373, 3763, 5103, 5365, 5642
6997
BsaAI 6 676, 1078, 2389, 6177, 6908
7467
BsaBI 4 1278, 2051, 4176, 6461
BsaHI 9 459, 512, 595, 781, 1199
2555, 3508, 6357, 6605
BsaI 5 1746, 2122, 3110, 3610, 5957
BsaWI 8 1261, 2495, 3909, 4136, 5203
5350, 6339, 6637
BseMII 9 981, 1139, 1842, 1951, 2886
2982, 5272, 5681, 5847
BseRI 3 3211, 3503, 4123
BseYI 9 1173, 2287, 2374, 2441, 2866
3063, 5301, 6352, 7110
BsgI 4 1861, 2471, 3526, 4482
BsiEI 7 1209, 1243, 2127, 4202, 4910
5334, 6512
BsiHKAI 9 1129, 1601, 1631, 2499, 3259
3634, 5311, 6715, 6905
BsmBI 3 978, 2206, 2553
BsmFI 8 499, 650, 818, 2113, 3011
3164, 3748, 6743
BsmI 4 1365, 1464, 2585, 3616
BspCNI 9 981, 1139, 1842, 1951, 2886
2982, 5272, 5681, 5847
BspHI 1 5717
BspMI 8 1976, 2820, 2878, 3180, 3899
6273, 6502, 6865
BsrBI 5 1212, 4345, 4928, 7217, 7611
BsrDI 4 2604, 5944, 6126, 6832
BsrGI 1 192
BssHII 5 2096, 2098, 3354, 3485, 7670
BssSI 4 3515, 3622, 5170, 7198
Bst1107I 1 3754
BstAPI 2 1854, 3073
BstEII 2 3176, 4092
BstXI 1 2593
Bsu36I 1 1138
BtgI 9 698, 1043, 1659, 1814, 2613
3292, 3444, 3468, 7038
BtgZI 7 687, 1885, 2829, 6892, 7043
7236, 7455
BtsI 5 1460, 1858, 2229, 3523, 6284
ClaI 1 1230
Csp6I 10 193, 556, 581, 636, 669
720, 877, 1040, 3244, 6911
DdeI 10 981, 1139, 1842, 1951, 2525
2886, 2982, 5272, 5681, 5847
DraI 6 1320, 2017, 3775, 3999, 5754
5773
DraIII 2 1078, 7464
DrdI 4 3442, 5099, 6627, 7418
EagI 2 1209, 6512
EarI 3 4875, 6955, 7165
Eco47III 3 2284, 3399, 4364
EcoICRI 2 1631, 3634
EcoO109I 5 1088, 2137, 2340, 3303, 3597
EcoRI 1 3782
EcoRV 1 1249
FspI 2 6110, 6706
HgaI 10 855, 1200, 1205, 1238, 1673
2555, 3422, 5099, 5677, 7696
HincII 3 1202, 1381, 1895
HindIII 1 1224
HpaI 1 1381
KasI 3 3508, 6357, 6605
KpnI 1 1039
MluI 1 1236
MmeI 5 1766, 1961, 5187, 5371, 7421
MscI 3 268, 3360, 6686
MslI 5 699, 2594, 2659, 3880, 7039
MunI 2 1370, 1914
NaeI 4 1096, 4069, 7106, 7570
NarI 3 3508, 6357, 6605
NcoI 5 698, 1043, 1659, 3444, 7038
NdeI 2 571, 4819
NgoMIV 4 1096, 4069, 7106, 7570
NheI 1 940
NotI 1 1208
NsiI 3 2405, 2583, 4808
NspI 9 143, 1651, 3007, 3022, 3127
3949, 4806, 4997, 7007
PacI 2 4799, 7729
PciI 3 143, 3949, 4997
PfoI 2 2154, 2670
PmeI 1 3774
PmlI 1 1078
PpuMI 2 2137, 3303
PsiI 3 1401, 4041, 7339
PspOMI 2 2609, 2915
PstI 5 1774, 1801, 2231, 2964, 6131
PvuI 1 1243
PvuII 7 1111, 1956, 2864, 2891, 2967
6350, 6710
RsaI 10 193, 556, 581, 636, 669
720, 877, 1040, 3244, 6911
RsrII 1 7121
SacI 2 1631, 3634
SacII 2 1814, 3468
SalI 1 1202
SapI 3 4874, 6955, 7165
SexAI 1 3659
SfcI 8 1774, 1801, 2231, 2964, 5262
5453, 6131, 7687
SfoI 3 3508, 6357, 6605
SgrAI 1 186
SmaI 5 1182, 1195, 1930, 2110, 4214
SmlI 7 1215, 3373, 3763, 5103, 5365
5642, 6997
SnaBI 1 676
SphI 3 1651, 3127, 7007
SrfI 1 1181
StyI 10 698, 1043, 1659, 2344, 3444
3480, 3498, 4324, 6415, 7038
TatI 5 192, 555, 635, 668, 719
TfiI 7 1920, 2461, 3569, 4176, 4972
7091, 7225
TspGWI 4 154, 3743, 4054, 4616
Tth111I 2 1748, 6721
Van91I 3 1058, 1077, 2155
XbaI 1 1267
XcmI 3 2037, 3628, 6415
XhoI 1 1215
XmaI 5 1182, 1195, 1930, 2110, 4214
XmnI 1 7719
ZraI 4 459, 512, 595, 781
Enzymes that do NOT cut molecule:
AflII AgeI AleI AscI AsiSI
BbvCI BlpI BsiWI BstBI EcoNI
FseI FspAI NruI PshAI SanDI
SbfI ScaI SfiI SpeI SspI
StuI SwaI
pNTAP-Shuttle-B质粒序列载体序列
pNTAP Shuttle B, 7736 bp version 016001 NOTE: The following sequence has been verified for accuracy at the junctions. The remainder of the sequence has been obtained from existing data. 1 CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG 51 GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG 101 TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA 151 GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG 201 GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG 251 CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA 301 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA 351 GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 401 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC 451 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 501 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT 551 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC 601 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG 651 GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 701 TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC 751 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 801 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC 851 CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC 901 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CTAGCATGAA 951 GCGACGATGG AAAAAGAATT TCATAGCCGT CTCAGCAGCC AACCGCTTTA 1001 AGAAAATCTC ATCCTCCGGG GCACTTGGAA GCGGTAGCGG TACCATGGAC 1051 GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG 1101 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC 1151 GGGAGCCCTC CGGCGGCTGC AAGCTGGGCT GCCCGGGCGG ATCCCCCGGG 1201 CGTCGACGCG GCCGCTCGAG CCGAAGCTTA TCGATACGCG TCCGATCGGA 1251 TATCCGATCC ACCGGATCTA GATAACTGAT CATAATCAGC CATACCACAT 1301 TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC 1351 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC 1401 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 1451 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA 1501 TCTTAACGCG GATCTGGGCG TGGTTAAGGG TGGGAAAGAA TATATAAGGT 1551 GGGGGTCTTA TGTAGTTTTG TATCTGTTTT GCAGCAGCCG CCGCCGCCAT 1601 GAGCACCAAC TCGTTTGATG GAAGCATTGT GAGCTCATAT TTGACAACGC 1651 GCATGCCCCC ATGGGCCGGG GTGCGTCAGA ATGTGATGGG CTCCAGCATT 1701 GATGGTCGCC CCGTCCTGCC CGCAAACTCT ACTACCTTGA CCTACGAGAC 1751 CGTGTCTGGA ACGCCGTTGG AGACTGCAGC CTCCGCCGCC GCTTCAGCCG 1801 CTGCAGCCAC CGCCCGCGGG ATTGTGACTG ACTTTGCTTT CCTGAGCCCG 1851 CTTGCAAGCA GTGCAGCTTC CCGTTCATCC GCCCGCGATG ACAAGTTGAC 1901 GGCTCTTTTG GCACAATTGG ATTCTTTGAC CCGGGAACTT AATGTCGTTT 1951 CTCAGCAGCT GTTGGATCTG CGCCAGCAGG TTTCTGCCCT GAAGGCTTCC 2001 TCCCCTCCCA ATGCGGTTTA AAACATAAAT AAAAAACCAG ACTCTGTTTG 2051 GATTTGGATC AAGCAAGTGT CTTGCTGTCT TTATTTAGGG GTTTTGCGCG 2101 CGCGGTAGGC CCGGGACCAG CGGTCTCGGT CGTTGAGGGT CCTGTGTATT 2151 TTTTCCAGGA CGTGGTAAAG GTGACTCTGG ATGTTCAGAT ACATGGGCAT 2201 AAGCCCGTCT CTGGGGTGGA GGTAGCACCA CTGCAGAGCT TCATGCTGCG 2251 GGGTGGTGTT GTAGATGATC CAGTCGTAGC AGGAGCGCTG GGCGTGGTGC 2301 CTAAAAATGT CTTTCAGTAG CAAGCTGATT GCCAGGGGCA GGCCCTTGGT 2351 GTAAGTGTTT ACAAAGCGGT TAAGCTGGGA TGGGTGCATA CGTGGGGATA 2401 TGAGATGCAT CTTGGACTGT ATTTTTAGGT TGGCTATGTT CCCAGCCATA 2451 TCCCTCCGGG GATTCATGTT GTGCAGAACC ACCAGCACAG TGTATCCGGT 2501 GCACTTGGGA AATTTGTCAT GTAGCTTAGA AGGAAATGCG TGGAAGAACT 2551 TGGAGACGCC CTTGTGACCT CCAAGATTTT CCATGCATTC GTCCATAATG 2601 ATGGCAATGG GCCCACGGGC GGCGGCCTGG GCGAAGATAT TTCTGGGATC 2651 ACTAACGTCA TAGTTGTGTT CCAGGATGAG ATCGTCATAG GCCATTTTTA 2701 CAAAGCGCGG GCGGAGGGTG CCAGACTGCG GTATAATGGT TCCATCCGGC 2751 CCAGGGGCGT AGTTACCCTC ACAGATTTGC ATTTCCCACG CTTTGAGTTC 2801 AGATGGGGGG ATCATGTCTA CCTGCGGGGC GATGAAGAAA ACGGTTTCCG 2851 GGGTAGGGGA GATCAGCTGG GAAGAAAGCA GGTTCCTGAG CAGCTGCGAC 2901 TTACCGCAGC CGGTGGGCCC GTAAATCACA CCTATTACCG GGTGCAACTG 2951 GTAGTTAAGA GAGCTGCAGC TGCCGTCATC CCTGAGCAGG GGGGCCACTT 3001 CGTTAAGCAT GTCCCTGACT CGCATGTTTT CCCTGACCAA ATCCGCCAGA 3051 AGGCGCTCGC CGCCCAGCGA TAGCAGTTCT TGCAAGGAAG CAAAGTTTTT 3101 CAACGGTTTG AGACCGTCCG CCGTAGGCAT GCTTTTGAGC GTTTGACCAA 3151 GCAGTTCCAG GCGGTCCCAC AGCTCGGTCA CCTGCTCTAC GGCATCTCGA 3201 TCCAGCATAT CTCCTCGTTT CGCGGGTTGG GGCGGCTTTC GCTGTACGGC 3251 AGTAGTCGGT GCTCGTCCAG ACGGGCCAGG GTCATGTCTT TCCACGGGCG 3301 CAGGGTCCTC GTCAGCGTAG TCTGGGTCAC GGTGAAGGGG TGCGCTCCGG 3351 GCTGCGCGCT GGCCAGGGTG CGCTTGAGGC TGGTCCTGCT GGTGCTGAAG 3401 CGCTGCCGGT CTTCGCCCTG CGCGTCGGCC AGGTAGCATT TGACCATGGT 3451 GTCATAGTCC AGCCCCTCCG CGGCGTGGCC CTTGGCGCGC AGCTTGCCCT 3501 TGGAGGAGGC GCCGCACGAG GGGCAGTGCA GACTTTTGAG GGCGTAGAGC 3551 TTGGGCGCGA GAAATACCGA TTCCGGGGAG TAGGCATCCG CGCCGCAGGC 3601 CCCGCAGACG GTCTCGCATT CCACGAGCCA GGTGAGCTCT GGCCGTTCGG 3651 GGTCAAAAAC CAGGTTTCCC CCATGCTTTT TGATGCGTTT CTTACCTCTG 3701 GTTTCCATGA GCCGGTGTCC ACGCTCGGTG ACGAAAAGGC TGTCCGTGTC 3751 CCCGTATACA GACTTGAGAG GGAGTTTAAA CGAATTCAAT AGCTTGTTGC 3801 ATGGGCGGCG ATATAAAATG CAAGGTGCTG CTCAAAAAAT CAGGCAAAGC 3851 CTCGCGCAAA AAAGAAAGCA CATCGTAGTC ATGCTCATGC AGATAAAGGC 3901 AGGTAAGCTC CGGAACCACC ACAGAAAAAG ACACCATTTT TCTCTCAAAC 3951 ATGTCTGCGG GTTTCTGCAT AAACACAAAA TAAAATAACA AAAAAACATT 4001 TAAACATTAG AAGCCTGTCT TACAACAGGA AAAACAACCC TTATAAGCAT 4051 AAGACGGACT ACGGCCATGC CGGCGTGACC GTAAAAAAAC TGGTCACCGT 4101 GATTAAAAAG CACCACCGAC AGCTCCTCGG TCATGTCCGG AGTCATAATG 4151 TAAGACTCGG TAAACACATC AGGTTGATTC ACATCGGTCA GTGCTAAAAA 4201 GCGACCGAAA TAGCCCGGGG GAATACATAC CCGCAGGCGT AGAGACAACA 4251 TTACAGCCCC CATAGGAGGT ATAACAAAAT TAATAGGAGA GAAAAACACA 4301 TAAACACCTG AAAAACCCTC CTGCCTAGGC AAAATAGCAC CCTCCCGCTC 4351 CAGAACAACA TACAGCGCTT CCACAGCGGC AGCCATAACA GTCAGCCTTA 4401 CCAGTAAAAA AGAAAACCTA TTAAAAAAAC ACCACTCGAC ACGGCACCAG 4451 CTCAATCAGT CACAGTGTAA AAAAGGGCCA AGTGCAGAGC GAGTATATAT 4501 AGGACTAAAA AATGACGTAA CGGTTAAAGT CCACAAAAAA CACCCAGAAA 4551 ACCGCACGCG AACCTACGCC CAGAAACGAA AGCCAAAAAA CCCACAACTT 4601 CCTCAAATCG TCACTTCCGT TTTCCCACGT TACGTCACTT CCCATTTTAA 4651 GAAAACTACA ATTCCCAACA CATACAAGTT ACTCCGCCCT AAAACCTACG 4701 TCACCCGCCC CGTTCCCACG CCCCGCGCCA CGTCACAAAC TCCACCCCCT 4751 CATTATCATA TTGGCTTCAA TCCAAAATAA GGTATATTAT TGATGATGTT 4801 AATTAACATG CATGGATCCA TATGCGGTGT GAAATACCGC ACAGATGCGT 4851 AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT 4901 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 4951 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 5001 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 5051 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 5101 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 5151 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 5201 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 5251 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 5301 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 5351 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 5401 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 5451 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 5501 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 5551 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 5601 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 5651 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 5701 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 5751 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 5801 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 5851 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA 5901 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 5951 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 6001 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 6051 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 6101 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGCCAT GAGATTATCA 6151 AAAAGGATCT TCACCTAGAT CCTTTTCACG TAGAAAGCCA GTCCGCAGAA 6201 ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG 6251 AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG 6301 CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC 6351 AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA 6401 TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGCTCTGAT 6451 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA 6501 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG 6551 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 6601 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 6651 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG 6701 TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 6751 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC 6801 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA 6851 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 6901 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 6951 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA 7001 AGGCGAGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 7051 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA 7101 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA 7151 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 7201 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 7251 CCTTCTTGAC GAGTTCTTCT GAATTTTGTT AAAATTTTTG TTAAATCAGC 7301 TCATTTTTTA ACCAATAGGC CGAAATCGGC ACCATCCCTT ATAAATCAAA 7351 AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC 7401 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT 7451 CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGTG 7501 GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT 7551 TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG 7601 AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT 7651 GCGCGTAACC ACCACACCCG CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC 7701 CATTCGCCAT TCAGGATCGA ATTAATTCTT AATTAA
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pNTAP-Shuttle-B质粒载体应用举例
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