| 出品公司: | Addgene |
|---|---|
| 载体名称: | pAAV-CA |
| 质粒类型: | 腺病毒载体； Cre-LoxP重组酶系统 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CAG (CMV enhancer/chicken β-actin promoter) |
| 载体大小: | 6060 bp |
| 5' 测序引物及序列: | XpAAVE-F (GCTAACCATGTTCATGCCTTC) |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: | -- |
| 产品目录号: | #69616 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV-CA 6060 bp DNA SYN
DEFINITION pAAV-CA
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6060
/organism="pAAV-CA"
/mol_type="other DNA"
misc_feature 239..526
/label="CAG_enhancer"
CDS 410..889
/label="ORF frame 2"
/translation="MPSTPPIDVNDGKWPAWHYAQYMTLWDFPTWQYIYVLVIAITMG
RGEPHVLLHSPHLPPLPTPNFVFIYFLIILCSDGGGGGGGARQAGRGGARGGAGRGGE
VRRQPIRAARSESFLLWRGGGGGGPIKSEARGGRESLRCLRPVPRSAPPRAARPGSD*"
CDS complement(998..1501)
/label="ORF frame 3"
/translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG
TASPAPRPVRSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT
KGPPGAL*"
misc_feature 1836..1855
/label="pCAG_F_primer"
/translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG
TASPAPRPVRSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT
KGPPGAL*"
CDS 2145..2888
/label="ORF frame 3"
/translation="MYAIRGYRMLELIDNQPLDYKICERLTGILNYVAPFTLCGYAAL
MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKSSSFPWLLAYVATW
ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHRYRALLE
RSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
misc_feature 2189..2776
/label="WPRE"
/translation="MYAIRGYRMLELIDNQPLDYKICERLTGILNYVAPFTLCGYAAL
MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKSSSFPWLLAYVATW
ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHRYRALLE
RSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
CDS 2290..2823
/label="ORF frame 1"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
terminator 2805..3283
/label="hGH_PA_terminator"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
rep_origin 3555..3861
/label="f1_origin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
misc_feature 4129..4151
/label="pGEX_3_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
promoter 4310..4338
/label="AmpR_promoter"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
gene 4380..5240
/label="Ampicillin"
/gene="Ampicillin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
CDS 4380..5240
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 5395..6014
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtAAGCT GGGctgcagg aatctagtta
181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
541 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
601 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
661 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
721 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
781 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
841 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
901 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
961 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
1021 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
1081 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
1141 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
1201 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
1261 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
1321 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
1381 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
1441 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
1501 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgTgc
1561 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggG cgaagcggtg
1621 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgcCgtcc
1681 ccttctccCt ctccagcctc ggggctgTcc gcGgggggac ggctgccttc gggggggacg
1741 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
1801 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggttA ttgtgctgtc
1861 tcatcatttt ggcaaagaat tgatttgata cgtacggttg aattcaTTAC CTCGTATAGG
1921 ATACTTTATA CGAAGTTATG CAGAATGGTA GCTGGATTGT AAGTACTATT AGCAATATGA
1981 AACCTCTTAA TAACCTCGTA TAGCATACAT TATACGAAGT AATAgatcta agcttgatat
2041 cgtcGACATA ACTTCGTATA AAGTATCCTA TACGAGGTAA TTTGCCTTAA CCCAGAAATT
2101 ATCAGTACTA TTCTTTAGAA TGGTGCAAAG AATTACTTCG TATAATGTAT GCTATACGAG
2161 GTTATCGCAT Gctagagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
2221 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
2281 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
2341 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
2401 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
2461 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
2521 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
2581 aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt
2641 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
2701 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
2761 gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg
2821 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct
2881 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat
2941 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg
3001 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc
3061 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc
3121 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt
3181 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt
3241 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac
3301 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
3361 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
3421 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
3481 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
3541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
3601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
3661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
3721 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
3781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
3841 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
3901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
3961 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
4021 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
4081 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
4141 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
4201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
4261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
4321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
4381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
4441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
4501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
4561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
4621 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
4681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
4741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
4801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
4861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
4921 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
4981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
5041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
5101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
5161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
5221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
5281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
5341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
5401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
5461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
5521 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
5581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
5641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
5701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
5761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
5821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
5881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
5941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
6001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pNTAP-Shuttle-B
- 下一篇：pAAV-CAG-Fluc
