| 质粒名称: | ERM-APEX2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79055 |
| 存储实验室: | Alice Ting |
|
载体骨架基本信息 |
|
| 载体名称: | pLX304 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7740 bp |
| 完整质粒大小: | 8694 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，细菌表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | APEX2 anchored to the cytosolic face of the ER membrane |
| 物种来源: | Soybean |
| 插入大小: | 954 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV immearly promotor |
| 标签/融合蛋白: |
P450氧化酶2C1的残基1-27（插入物上的N端）
V5（插入件上的C端子）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | ERM-APEX2 was a gift from Alice Ting (Addgene plasmid # 79055 ; http://n2t.net/addgene:79055 ; RRID:Addgene_79055) |
| 参考文献部分: | Directed evolution of APEX2 for electron microscopy and proximity labeling. Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. 10.1038/nmeth.3179 PubMed 25419960 |
买家导航
ERMAPEX2载体基本信息
ERMAPEX2载体图谱质粒图谱和多克隆位点信息
ERMAPEX2载体简介
ERMAPEX2载体序列
LOCUS ERM-APEX2 8694 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Soybean APEX2 anchored to cytosolic face of ER membrane with
N-terminal targeting sequence of residues 1-27 of ER-resident P450
oxidase 2C1..
ACCESSION .
VERSION .
KEYWORDS ERM-APEX2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8694)
AUTHORS Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK,
Ting AY
TITLE Directed evolution of APEX2 for electron microscopy and proximity
labeling.
JOURNAL Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
PUBMED 25419960
REFERENCE 2 (bases 1 to 8694)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8694
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 105..408
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 409..612
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 563..583
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 635..659
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 836..1582
/codon_start=1
/product="soybean ascorbate peroxidase, engineered to serve
as a monomeric tag for electron microscopy and live-cell
proteomics (Lam et al., 2015)"
/label=APEX2
/note="more active than the original APEX due to the A134P
mutation"
/translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
YAADEDAFFADYAEAHQKLSELGFADA"
CDS 1583..1624
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
protein_bind complement(1632..1656)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1658..1699
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 1741..2329
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1794..1814)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2212..2223)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 2401..2634
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 2712..2833
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2749..2768)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2803..2822
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 2873..3008
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2935..2954
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3028..3047)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3029..3047)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3057..3074)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3057..3073)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3066..3088)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3215..3670
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3302..3321)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3512..3533
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3696..3800
/gene="bla"
/label=AmpR promoter
CDS 3801..4661
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4019..4038)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4832..5420
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5321..5340
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5574..5591
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5708..5729
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5744..5774
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5782..5798
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5787..5809
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5806..5822
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5806..5822
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 5841..5861
/label=T3
/note="T3 promoter, forward primer"
promoter 5843..5861
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 5889..6115
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6116..6296
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6343..6468
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6961..7194
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7379..7423
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 7598..8098
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 8017..8035
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 8110..8505
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 8565..8682
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 agggacagca gagatccact ttggcgccgg ctcgaggggg cccggggtta ttaatagtaa
61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
601 ataagcagag ctctctggct aactgtcggg atcaacaagt ttgtacaaaa aagcaggctt
661 cgaaggagat agaaccatgg accctgtggt cgtgctgggg ctgtgcctga gctgtctgct
721 gctgctgagc ctgtggaagc agtcctacgg aggcggggga tcaggaagcg gcggccgcgg
781 atcaggaagc ggatcaggaa gcggatcagg aagcggatca ggaagcggat caggaggaaa
841 gtcttaccca actgtgagtg ctgattacca ggacgccgtt gagaaggcga agaagaagct
901 cagaggcttc atcgctgaga agagatgcgc tcctctaatg ctccgtttgg cattccactc
961 tgctggaacc tttgacaagg gcacgaagac cggtggaccc ttcggaacca tcaagcaccc
1021 tgccgaactg gctcacagcg ctaacaacgg tcttgacatc gctgttaggc ttttggagcc
1081 actcaaggcg gagttcccta ttttgagcta cgccgatttc taccagttgg ctggcgttgt
1141 tgccgttgag gtcacgggtg gacctaaggt tccattccac cctggaagag aggacaagcc
1201 tgagccacca ccagagggtc gcttgcccga tcccactaag ggttctgacc atttgagaga
1261 tgtgtttggc aaagctatgg ggcttactga ccaagatatc gttgctctat ctgggggtca
1321 cactattgga gctgcacaca aggagcgttc tggatttgag ggtccctgga cctctaatcc
1381 tcttattttc gacaactcat acttcacgga gttgttgagt ggtgagaagg aaggtctcct
1441 tcagctacct tctgacaagg ctcttttgtc tgaccctgta ttccgccctc tcgttgacaa
1501 atatgcagcg gacgaagatg ccttctttgc tgattacgct gaggctcacc aaaagctttc
1561 cgagcttggg tttgctgatg ccggcaagcc catccccaac cccctgctgg gcctggacag
1621 cacctgataa gacccagctt tcttgtacaa agtggttggt aagcctatcc ctaaccctct
1681 cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac
1741 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
1801 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
1861 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
1921 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
1981 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
2041 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
2101 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
2161 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
2221 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
2281 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
2341 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
2401 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
2461 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
2521 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
2581 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
2641 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
2701 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
2761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
2821 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
2881 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
2941 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
3001 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
3061 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
3121 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
3181 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
3241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
3301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
3361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
3421 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
3481 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
3541 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
3601 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
3661 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
3721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
3781 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
3841 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
3901 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
3961 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4021 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
4081 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
4141 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
4201 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
4261 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
4321 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
4381 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
4441 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
4501 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
4561 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
4621 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
4681 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
4741 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
4801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
4861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
4921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
4981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
5221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
5281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
5341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
5401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
5461 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
5521 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
5581 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
5641 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
5701 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
5761 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
5821 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
5881 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
5941 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
6001 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
6061 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
6121 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
6181 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
6241 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
6301 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
6361 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
6421 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
6481 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
6541 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
6601 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
6661 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
6721 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
6781 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
6841 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
6901 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
6961 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
7021 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
7081 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
7141 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
7201 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
7261 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
7321 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
7381 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
7441 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
7501 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
7561 atcgtttcag acccacctcc caaccccgag gggacccttg cgccttttcc aaggcagccc
7621 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
7681 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
7741 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
7801 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
7861 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
7921 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
7981 tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
8041 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg
8101 ggtaccacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
8161 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
8221 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
8281 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
8341 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
8401 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
8461 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggcctgca gctgcagtag
8521 taagaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
8581 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
8641 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttac
//
