LOCUS pCAMBIA1281Z 11379 bp ds-DNA circular SYN 01-十二月-2013
DEFINITION Agrobacterium binary vector for plant transformation, with
hygromycin- and chloramphenicol-resistance and LacZ-GUS genes plus
the pUC8 MCS.
ACCESSION AF234294
VERSION .
KEYWORDS pCAMBIA1281Z
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT The GenBank record was corrected by inserting a G at position 4743.
FEATURES Location/Qualifiers
source 1..11379
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS join(2..16,207..2024)
/codon_start=1
/product="beta-glucuronidase"
/label="GUS"
/label="This version of the gusA gene has a 5' extension
with a catalase intron to ensure expression in plants but
not bacteria."
/protein_id="
"
intron 17..206
/label="cat1 intron"
/label="castor bean catalase intron, modified"
CDS 2031..2048
/codon_start=1
/product="6xHis affinity tag"
/label="6xHis"
/translation="HHHHHH"
terminator 2083..2335
/label="NOS terminator"
/label="nopaline synthase terminator and poly(A) signal"
misc_feature 2357..2381
/label="RB T-DNA repeat"
/label="right border repeat from nopaline C58 T-DNA"
CDS 3681..4310
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label="pVS1 StaA"
CDS 4739..5812
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label="pVS1 RepA"
/label="
"
/protein_id="
"
misc_feature 6416..6556
/label="bom"
/label="basis of mobility region from pBR322"
rep_origin complement(6742..7330)
/direction=LEFT
/label="ori"
/label="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7601..8260)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label="CmR"
/label="confers resistance to chloramphenicol"
misc_feature 8788..8812
/label="LB T-DNA repeat"
/label="left border repeat from nopaline C58 T-DNA"
polyA_signal 8890..9064
/label="CaMV poly(A) signal"
/label="cauliflower mosaic virus polyadenylation signal"
CDS complement(9104..10129)
/codon_start=1
/product="hygromycin B phosphotransferase"
/label="HygR"
/label="confers resistance to hygromycin"
promoter complement(10197..10874)
/label="CaMV 35S promoter (enhanced)"
/label="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
promoter 11088..11118
/label="lac promoter"
/label="promoter for the E. coli lac operon"
protein_bind 11126..11142
/bound_moiety="lac repressor encoded by lacI"
/label="lac operator"
/label="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 11150..11166
/label="M13 rev"
/label="common sequencing primer, one of multiple similar
variants"
CDS 11162..11359
/codon_start=1
/gene="lacZ (fragment)"
/product="LacZ-alpha fragment of?beta-galactosidase"
/label="lacZ-alpha"
/protein_id="
"
misc_feature 11176..11211
/label="MCS"
/label="multiple cloning site from pUC8"
primer_bind complement(11215..11231)
/label="M13 fwd"
/label="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
7381 ctcacgttaa gggattttgg tcatgcatga tatatctccc aatttgtgta gggcttatta
7441 tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg
7501 tgcttagtgc atctaatcgc ttgagttaac gccggcgaag cggcgtcggc ttgaacgaat
7561 ttctagctag aggatcgcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
7621 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
7681 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
7741 ccattgtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
7801 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
7861 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
7921 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
7981 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
8041 ggaactccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
8101 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
8161 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
8221 atatatcaac ggtggtatat ccagtgattt ttttctccat gatgtttaac tttgttttag
8281 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
8341 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca
8401 taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
8461 ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag
8521 gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt
8581 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
8641 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
8701 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
8761 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
8821 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
8881 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
8941 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
9001 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
9061 gaaactcgag cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg
9121 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
9181 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
9241 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
9301 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg
9361 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
9421 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
9481 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
9541 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
9601 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
9661 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
9721 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct
9781 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
9841 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca
9901 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
9961 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
10021 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
10081 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
10141 ccccgggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag
10201 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt
10261 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
10321 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
10381 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
10441 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
10501 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
10561 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
10621 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
10681 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
10741 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
10801 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
10861 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
10921 agacgagagt gtcgtgctcc accatgttgg ccaatacgca aaccgcctct ccccgcgcgt
10981 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
11041 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
11101 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
11161 tatgaccatg attacgaatt cccggggatc cgtcgacctg cagccaagct tggcactggc
11221 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
11281 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
11341 ccaacagttg cgcagcctga agcctaggga ggagtccac
//