| 质粒名称: | pAAV2/2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 104963 |
| 存储实验室: | Melina Fan |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 with additional elements |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 7468 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达,AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rep2/Cap2 |
| 物种来源: | parvovirus |
| 插入大小: | 4090 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | p5 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13 Reverse |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV2/2 was a gift from Melina Fan (Addgene plasmid # 104963 ; http://n2t.net/addgene:104963 ; RRID:Addgene_104963) |
买家导航
pAAV22载体基本信息
pAAV22载体图谱质粒图谱和多克隆位点信息
pAAV22载体简介
pAAV22载体序列
LOCUS pAAV2/2 7468 bp ds-DNA circular SYN 25-SEP-2018
DEFINITION RepCap plasmid for AAV production, serotype 2 (VERSION 3 - STILL
TESTING, DO NOT USE).
ACCESSION .
VERSION .
KEYWORDS pAAV2/2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7468)
TITLE AAV Packaging
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7468)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 25, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7468
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 132..151
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 385..402
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 519..540
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 555..585
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 593..609
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 598..620
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 617..633
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 617..633
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 683..699
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 683..699
/label=pBluescriptSK
/note="For pBluescript vector"
protein_bind 713..760
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind complement(5336..5353)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5336..5352)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5345..5367)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin complement(5493..5948)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5631..5652)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5842..5861
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 5975..6079
/gene="bla"
/label=AmpR promoter
CDS 6080..6940
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6298..6317)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7111..231
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgccgatatc
661 gttaacgccc cgcgccggcc gctctagaac tagtggatcc cccggaagat cagaagttcc
721 tattccgaag ttcctattct ctagaaagta taggaacttc tgatctattc gagctcggta
781 cccctagagt cctgtattag aggtcacgtg agtgttttgc gacattttgc gacaccatgt
841 ggtcacgctg ggtatttaag cccgagtgag cacgcagggt ctccattttg aagcgggagg
901 tttgaacgcg cagccgccat gccggggttt tacgagattg tgattaaggt ccccagcgac
961 cttgacgagc atctgcccgg catttctgac agctttgtga actgggtggc cgagaaggaa
1021 tgggagttgc cgccagattc tgacatggat ctgaatctga ttgagcaggc acccctgacc
1081 gtggccgaga agctgcagcg cgactttctg acggaatggc gccgtgtgag taaggccccg
1141 gaggcccttt tctttgtgca atttgagaag ggagagagct acttccacat gcacgtgctc
1201 gtggaaacca ccggggtgaa atccatggtt ttgggacgtt tcctgagtca gattcgcgaa
1261 aaactgattc agagaattta ccgcgggatc gagccgactt tgccaaactg gttcgcggtc
1321 acaaagacca gaaatggcgc cggaggcggg aacaaggtgg tggatgagtg ctacatcccc
1381 aattacttgc tccccaaaac ccagcctgag ctccagtggg cgtggactaa tatggaacag
1441 tatttaagcg cctgtttgaa tctcacggag cgtaaacggt tggtggcgca gcatctgacg
1501 cacgtgtcgc agacgcagga gcagaacaaa gagaatcaga atcccaattc tgatgcgccg
1561 gtgatcagat caaaaacttc agccaggtac atggagctgg tcgggtggct cgtggacaag
1621 gggattacct cggagaagca gtggatccag gaggaccagg cctcatacat ctccttcaat
1681 gcggcctcca actcgcggtc ccaaatcaag gctgccttgg acaatgcggg aaagattatg
1741 agcctgacta aaaccgcccc cgactacctg gtgggccagc agcccgtgga ggacatttcc
1801 agcaatcgga tttataaaat tttggaacta aacgggtacg atccccaata tgcggcttcc
1861 gtctttctgg gatgggccac gaaaaagttc ggcaagagga acaccatctg gctgtttggg
1921 cctgcaacta ccgggaagac caacatcgcg gaggccatag cccacactgt gcccttctac
1981 gggtgcgtaa actggaccaa tgagaacttt cccttcaacg actgtgtcga caagatggtg
2041 atctggtggg aggaggggaa gatgaccgcc aaggtcgtgg agtcggccaa agccattctc
2101 ggaggaagca aggtgcgcgt ggaccagaaa tgcaagtcct cggcccagat agacccgact
2161 cccgtgatcg tcacctccaa caccaacatg tgcgccgtga ttgacgggaa ctcaacgacc
2221 ttcgaacacc agcagccgtt gcaagaccgg atgttcaaat ttgaactcac ccgccgtctg
2281 gatcatgact ttgggaaggt caccaagcag gaagtcaaag actttttccg gtgggcaaag
2341 gatcacgtgg ttgaggtgga gcatgaattc tacgtcaaaa agggtggagc caagaaaaga
2401 cccgccccca gtgacgcaga tataagtgag cccaaacggg tgcgcgagtc agttgcgcag
2461 ccatcgacgt cagacgcgga agcttcgatc aactacgcag acaggtacca aaacaaatgt
2521 tctcgtcacg tgggcatgaa tctgatgctg tttccctgca gacaatgcga gagaatgaat
2581 cagaattcaa atatctgctt cactcacgga cagaaagact gtttagagtg ctttcccgtg
2641 tcagaatctc aacccgtttc tgtcgtcaaa aaggcgtatc agaaactgtg ctacattcat
2701 catatcatgg gaaaggtgcc agacgcttgc actgcctgcg atctggtcaa tgtggatttg
2761 gatgactgca tctttgaaca ataaatgatt taaatcaggt atggctgccg atggttatct
2821 tccagattgg ctcgaggaca ctctctctga aggaataaga cagtggtgga agctcaaacc
2881 tggcccacca ccaccaaagc ccgcagagcg gcataaggac gacagcaggg gtcttgtgct
2941 tcctgggtac aagtacctcg gacccttcaa cggactcgac aagggagagc cggtcaacga
3001 ggcagacgcc gcggccctcg agcacgacaa agcctacgac cggcagctcg acagcggaga
3061 caacccgtac ctcaagtaca accacgccga cgcggagttt caggagcgcc ttaaagaaga
3121 tacgtctttt gggggcaacc tcggacgagc agtcttccag gcgaaaaaga gggttcttga
3181 acctctgggc ctggttgagg aacctgttaa gacggctccg ggaaaaaaga ggccggtaga
3241 gcactctcct gtggagccag actcctcctc gggaaccgga aaggcgggcc agcagcctgc
3301 aagaaaaaga ttgaattttg gtcagactgg agacgcagac tcagtacctg acccccagcc
3361 tctcggacag ccaccagcag ccccctctgg tctgggaact aatacgatgg ctacaggcag
3421 tggcgcacca atggcagaca ataacgaggg cgccgacgga gtgggtaatt cctcgggaaa
3481 ttggcattgc gattccacat ggatgggcga cagagtcatc accaccagca cccgaacctg
3541 ggccctgccc acctacaaca accacctcta caaacaaatt tccagccaat caggagcctc
3601 gaacgacaat cactactttg gctacagcac cccttggggg tattttgact tcaacagatt
3661 ccactgccac ttttcaccac gtgactggca aagactcatc aacaacaact ggggattccg
3721 acccaagaga ctcaacttca agctctttaa cattcaagtc aaagaggtca cgcagaatga
3781 cggtacgacg acgattgcca ataaccttac cagcacggtt caggtgttta ctgactcgga
3841 gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga tgcctcccgc cgttcccagc
3901 agacgtcttc atggtgccac agtatggata cctcaccctg aacaacggga gtcaggcagt
3961 aggacgctct tcattttact gcctggagta ctttccttct cagatgctgc gtaccggaaa
4021 caactttacc ttcagctaca cttttgagga cgttcctttc cacagcagct acgctcacag
4081 ccagagtctg gaccgtctca tgaatcctct catcgaccag tacctgtatt acttgagcag
4141 aacaaacact ccaagtggaa ccaccacgca gtcaaggctt cagttttctc aggccggagc
4201 gagtgacatt cgggaccagt ctaggaactg gcttcctgga ccctgttacc gccagcagcg
4261 agtatcaaag acatctgcgg ataacaacaa cagtgaatac tcgtggactg gagctaccaa
4321 gtaccacctc aatggcagag actctctggt gaatccgggc ccggccatgg caagccacaa
4381 ggacgatgaa gaaaagtttt ttcctcagag cggggttctc atctttggga agcaaggctc
4441 agagaaaaca aatgtggaca ttgaaaaggt catgattaca gacgaagagg aaatcaggac
4501 aaccaatccc gtggctacgg agcagtatgg ttctgtatct accaacctcc agagaggcaa
4561 cagacaagca gctaccgcag atgtcaacac acaaggcgtt cttccaggca tggtctggca
4621 ggacagagat gtgtaccttc aggggcccat ctgggcaaag attccacaca cggacggaca
4681 ttttcacccc tctcccctca tgggtggatt cggacttaaa caccctcctc cacagattct
4741 catcaagaac accccggtac ctgcgaatcc ttcgaccacc ttcagtgcgg caaagtttgc
4801 ttccttcatc acacagtact ccacgggaca ggtcagcgtg gagatcgagt gggagctgca
4861 gaaggaaaac agcaaacgct ggaatcccga aattcagtac acttccaact acaacaagtc
4921 tgttaatgtg gactttactg tggacactaa tggcgtgtat tcagagcctc gccccattgg
4981 caccagatac ctgactcgta atctgtaatt gcttgttaat caataaaccg tttaattcgt
5041 ttcagttgaa ctttggtctc tgcgtatttc tttcttatct agtttccatg gctacgtaga
5101 taagtagcat ggcgggttaa tcattaacta cagcccgggc gtttaaacag cgggcggagg
5161 ggtggagtcg tgacgtgaat tacgtcatag ggttagggag agtcctgtat tagaggtcac
5221 gtgagtgttt tgcgacattt tgcgacacca tgtggtcacg ctgggtattt aagcccgagt
5281 gagcacgcag ggtctccatt ttgagcggga ggtttgaacg agcgctggcg cgctcactgg
5341 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
5401 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
5461 cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa
5521 attcgcgtta aatttttgtt aaatcagctc atttttttaa ccaataggcc gaaatcggca
5581 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
5641 acaagagtcc actattaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
5701 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
5761 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
5821 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
5881 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
5941 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
6001 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
6061 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
6121 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
6181 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
6241 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
6301 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
6361 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
6421 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
6481 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
6541 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
6601 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
6661 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
6721 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
6781 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
6841 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
6901 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
6961 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
7021 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
7081 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
7141 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
7201 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
7261 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
7321 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
7381 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
7441 gtgcacacag cccagcttgg agcgaacg
//
