pOBCol3.6-GFPtpz

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

pOBCol3.6GFPtpz载体基本信息

质粒名称: pOBCol3.6-GFPtpz 
出品公司: 爱迪基因
目录编号: 110211
存储实验室: David Rowe

载体骨架基本信息
载体名称: pBC SK+
载体出品公司: Stratagene
载体抗性: 氯霉素
空载体大小: 3400 bp
完整质粒大小: 9600 bp
载体修饰: --
载体类型: 哺乳细胞表达
筛选标记: --
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: eGFPtopaz,Topaz,YFP
物种来源: A. victoria
插入大小: 720 bp
基因突变: --
GenBank编号 --
启动子 3.6 kb rat type 1 collagen + first intron
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: Sac2(未破坏)
3' 克隆位点: Sal1 (未破坏)
5' 测序引物 T3
3' 测序引物 T7

文献引用方法
材料和方法部分: pOBCol3.6-GFPtpz was a gift from David Rowe (Addgene plasmid # 110211 ; http://n2t.net/addgene:110211 ; RRID:Addgene_110211)
参考文献部分: Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler AC, Rowe D. J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 10.1359/jbmr.2002.17.1.15. 10.1359/jbmr.2002.17.1.15 PubMed 11771662

pOBCol3.6GFPtpz载体图谱质粒图谱和多克隆位点信息

pOBCol3.6-GFPtpz质粒图谱

pOBCol3.6GFPtpz载体简介

pOBCol3.6GFPtpz载体序列

LOCUS       pOBCol3.6-GFPtpz                9671 bp ds-DNA     circular SYN 07-AUG-2018
DEFINITION  Labels immature osteoblasts and connective tissue cells expressing 
            type 1 collagen..
ACCESSION   .
VERSION     .
KEYWORDS    pOBCol3.6-GFPtpz
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler
            AC, Rowe D
  TITLE     Use of type I collagen green fluorescent protein transgenes to 
            identify subpopulations of cells at different stages of the 
            osteoblast lineage.
  JOURNAL   J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 
            10.1359/jbmr.2002.17.1.15.
  PUBMED    11771662
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     480..499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     733..750
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    867..888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        903..933
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    941..957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     946..968
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     965..981
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     965..981
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1000..1020
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1002..1020
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     6294..6310
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6294..6310
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      6377..6386
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6383..7102
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6428..6449)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6689..6708)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7036..7057
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7139..7363
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7378..7394)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7379..7395)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7419..7438)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7420..7438)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7448..7465)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7448..7464)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7457..7479)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7606..8061
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7693..7712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7903..7924
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8087..8191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(8680..9339)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9254..9273
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(9340..9442)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      9662..579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
       61 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      121 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      601 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      661 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      721 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      781 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      841 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      901 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      961 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     1021 gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaatatagaa gccaaggatt
     1081 tcaagggttt ccttttctct cttcttcttt ttttttcttt ttctttttcc tgagatggag
     1141 tttccttttg tagccctgac tgtcctggaa ttcactctat agaccaggct agcctcacac
     1201 ttagtgatct gcctgcctct gcctcttggg tgctcaggat tcaaggcatg aaccaccact
     1261 acccgaccag ggatttctta cacacttctg actggactaa ccaggaaagc agagagggag
     1321 acaggaagaa aatgctcaga aggaaggagt aggattggag gtgagctggg ggaacccaga
     1381 ctgagccgtg cagaagacaa ggaagaagaa agccacccac acacctagga tccacccaca
     1441 gattttgctc tgggtacccc tgtctggaga ctgtagggct ttgtgatgga gggtggggta
     1501 tgcttcatgc cccgtgccct ttactccaga cctaaatgcc cacccccaca tacagctgct
     1561 cgctctctct ctcccctgcc cttctcccaa gagaccagtt ctccatccct ggtctgcagc
     1621 caaggctggg ggcagaagaa ctttctggag gatttgagtg agaaaagcaa gagagcctca
     1681 agtagggact ggaacctctg ggaagggagt gcagaggaga cccgggtatg tgccctacct
     1741 ggtacattta tacctgggca gcctctgctc ctgttccaga cttcagagcc cagacgggtc
     1801 ctctccctcc ctcatgaggg gaaacatttg gggaaatttg gagagagaca gaactcagag
     1861 ctcagcactt tcctctttct gtttttcttc ttgaggaatt ttttccccca actgctgatg
     1921 actttaccat tcttgggggt gggggggtgg agattctggc ttttgctccc cctacactcc
     1981 aagtgccgga caaagcccta cattccacaa gaagccaggg cttcagagtt tcctaaagat
     2041 gaggtggcgt ggcgagtctc ctccctctcc cagctccaac tccccctccc ccagtctcca
     2101 gccctcagcc tggccaggga ggccccgcca ggctgggagg agaccccaag cacattcttc
     2161 ctctcgctgt catgctgcag aaattaaaga cacatctctg agctgggtac ccgccaatcg
     2221 tttcaagttg agaagtggca gaggaggtcc cgagcttcag ctccatgcca cgtgtaaagg
     2281 aagcttggaa cccactgccc acaactcctg gggcaaaaac ctggagtcag acatggggtg
     2341 aaggctgtca cacggcacag acacgtcaag cacccccccc aattctagta gtctcctagc
     2401 ctccaccaga accccagacc cttgatgtgg cagtcaccag tccacacctg ttaggctctt
     2461 gtctcttctt ccagatgagc ctggggggcg ggggggtgct agatcaggag cagggaaaag
     2521 tagctttgga taagtgcttt tcccaataca aaacccaaca aagagtgggc agatcacact
     2581 gtgtagtgct tcgtggaacc ctaccctaga caactgcctt gaacacctat tccctctgat
     2641 gtacaccatc cccgtccact gttagggagt gggcatcctt tggaactgac cactgtggaa
     2701 ggcaggactt tactgagttc cggaactacc atctcagctt ctcagcccca gccttaccct
     2761 acaggcactg gcataggcgg gggcagatcc tgggccacaa gtcactgcca catggttggg
     2821 ataattgatg aagtcctgtc cttccattgc tgtctccagt tctgcttctc tggaaactct
     2881 atatttttcc ctttaattat agcctctgca gtctccctct gccaccccac ccgcaccgct
     2941 tagcctaact gcccacggcc agcgacgtgg ctccctcccc ttctgctccc ttggtctttt
     3001 ttattttttt ttctttgcct tcgttgcaca aaactagctc agggagggcg tgaagggggg
     3061 gggagcaatg gaatcttgga tggtttggag gaggcgggac tccttgcttc cacgtttaca
     3121 gctctgaaga cggctgtggg ggaagtgata caggacgtct atgggccctg agaggagacc
     3181 cctatgcttc cctgccaccc acacagttta acaaaatgaa gttcctaagt agagtggggg
     3241 tcaggcagag cacctttgca gggttgatgg gagcccaggg aaagaaagga cactgtcttt
     3301 tagggacaca tttaaatata agccactttt cttgggggac gacaaatgac cctttcctga
     3361 ttgcagaggt ggggaacaat ggctgagatt ttcagcaaag aagcgaggac atgaggagta
     3421 gccttcaaat aaagtcactc agctaccaaa aacaagtttc tgccacacac cgagttcacc
     3481 taggtgtccc cagaccagat ccaagtacag taaggaaagc aggttctcta cagagagaac
     3541 acggctctat ggccaatgcc ttctacctgc tctttctgga ttgatactgc tacctaagag
     3601 ggcctctaac caattcctgg ctgtagccac agctgacaca agaccttttt ctaagacatc
     3661 cctggtcaca ggcctcctgt agcaaattcc agccctggga tggaggtggt caggaaagag
     3721 tttatacaag aagacccagg ccacagcttt aaggactcag aaacccccct gcccacacgg
     3781 ctgcccatca taacgcagaa ggtttcttct ggaaggacaa ggatgtcaaa cttctcccca
     3841 agcctaatcc tcagagatgt ctccctctgt tacacctggg gctggagaaa ggtgggtctt
     3901 tcatggagcc acattcatgg cagaacagat agccacccca ctcctttcaa acaaccacat
     3961 atctgactct tagtatctgt gaagagatgt ctaatttgtt cccaaatatt cctaccctgc
     4021 atacctgggc ccacaccatg aggtattctc ctccctctaa cagtcacatc tgcttagctg
     4081 cctggttctt cggatttgga gagatgcttg cctaacttat tcttccttag gtcttcccaa
     4141 ggatgccaga aagactatga gacatggcca agaggacctt ttcccaattg tgcctgacac
     4201 tgaacccttt gtaatgttcc ccaactcaga ttcccaattc tacatccttc tgatttgagg
     4261 tcccagaagg aaagtgcaag gggcatcccc tacccacaat cagtatatcg agggcccagc
     4321 cacactcagt gatagcacct ctggcccatg tagatctggg ggacaagggt ggcagaattg
     4381 caaagggggg agggggctgg gtggactcct ttcccttcct ttccctcctc ccccctcttc
     4441 gttccaaatt gggggccggg ccaggcagtt ctgattggct gggggccggg ctgctggctc
     4501 cccctctcca agaggcaggg ttcctcccag ccctcctcca tcaggatggt ataaaagggg
     4561 cccaggccag tcgtcggagc agacgggagt ttcacctccg gacggagcag gaggcacacg
     4621 gagtgaggcc acgcatgagc cgaagctaac cccccacccc agccgcaaag agtctacatg
     4681 tctagggtct agaggcggcc gccttcagct ttgtggacct ccggctcctg ctcctcttag
     4741 gggccactgc cctcctgacg catggccaag aagacagtaa gttccaaact ctggggaggt
     4801 tatggtttct tgctatcgcg ttctgcatag gggtcctagc aataaagact taaacggaag
     4861 cgcggggtgc tccagagtta ggagggatga gaaggtgatg gagaggaact gggaagagag
     4921 aggtcaccct cgcgagcaga ggagagtgag cggatctcag aagtggagcc ctggttgaga
     4981 tggcacggca ggaaatttca agcatctatc tgtagcgcga cgggaaaagt gggaatctgg
     5041 gcaggggttg ggtcagagta cgtcgaagat caaataagac ctctggaata gagattcgaa
     5101 gctcaagatg taaaagcaat caaaaaggct gcgggatgat tcataaggaa agattgtttg
     5161 ccctctctgc aggctagaca gtgttgctgg ggcctccggg gtgctgggct ctgggagtgg
     5221 ggtgggggga ggaagggacg cacggagagg gcgggttgct ggatgagaaa gtttggctaa
     5281 gacttgactg ggcgcggtcc ctgtgcctcc tgctggtcaa ccttgagcac tacgagtcct
     5341 ggtacagtac tttacagcgc gcgggcttaa gagggaaaac ctgggctatt gccgggcctg
     5401 gaccctcatc tccagactag gaaaataaaa gcacagatgg atgtgatagc tttggtcttt
     5461 gaaagtgacc caggccagta aaattgggtc cgccccgtgc attgcttagc gttgcccgcc
     5521 acctagtggt tgctttgatc cggtgctaga gaggtgggcg tggttaacta atcattgcta
     5581 tttgtagact tgagttcttt ctttggctaa ggagtgaccc caaagctcta ctggctttga
     5641 aggaaggacc gggcaagctt tgattcctgg gagcggagag ttagggtgta tctaacttta
     5701 gggatatatt ttactactct ggacaaggct ccacggaagt caccctcctc cattttagcc
     5761 ccgcccattc cgtcccacct gcccaaggaa aggaggtggg accagggctg gaatttccct
     5821 tctctcctcc cacccccgcc tccggtctcc ccgcaaacca cgtcccacaa acttccacct
     5881 actttgtagg agccctccta ctctccgtca ggaggacttc tatttcattt ggttcccgcg
     5941 cgcgtgggct gaagttgagt ttggaagcaa ccccagtcta aagctttgtt taaattcctg
     6001 ggaacttgga agtaacagcc ctggccaggc ttcttggtgc tgtcactgaa cgccgttaca
     6061 gagcagacct gcatttaagg atttaaggga agaaaatagg ggttggggga agatgtggga
     6121 agtggaaaat ctacgtgcac ctcttagaca ggggaaaggg tgagtaaggc tgggatccgg
     6181 tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc taggcctgta
     6241 cggaagtgtt acttctgctc taaaagctgc tgcaggtctt agttaactaa gactctagaa
     6301 ctagtggatc ccccgggctg caggaattcg atatcaagct tatcgataag cttggcattc
     6361 cggtactgtt ggtaaagcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     6421 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6481 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6541 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgc agtgcttcgc
     6601 ccgctacccc gaccacatgc gccagcacga cttcttcaag tccgccatgc ccgaaggcta
     6661 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6721 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6781 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6841 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6901 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6961 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     7021 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     7081 catggacgag ctgtacaagt aactcgctag agtcggggcg gccgggcgag ctcgaattct
     7141 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7201 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     7261 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7321 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggaattccg agcttatcga
     7381 taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg
     7441 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7501 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7561 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     7621 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7681 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7741 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7801 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7861 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7921 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7981 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     8041 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8161 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8221 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8281 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8341 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8401 ttgatcagaa aaaaaggatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa
     8461 actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
     8521 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt
     8581 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
     8641 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     8701 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     8761 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     8821 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     8881 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     8941 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     9001 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     9061 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     9121 taattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     9181 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     9241 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     9301 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     9361 aaatctcgac aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     9421 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     9481 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     9541 ttattcggtc gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9661 cttgagatcc t
//

pOBCol3.6GFPtpz载体图谱质粒图谱pdf版和相关资料下载

pOBCol3.6GFPtpz载体质粒应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng 沪ICP备2021037978号-1
欢迎您的访问和咨询....