质粒名称: | pOBCol3.6-GFPtpz |
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出品公司: | 爱迪基因 |
目录编号: | 110211 |
存储实验室: | David Rowe |
载体骨架基本信息 |
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载体名称: | pBC SK+ |
载体出品公司: | Stratagene |
载体抗性: | 氯霉素 |
空载体大小: | 3400 bp |
完整质粒大小: | 9600 bp |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | eGFPtopaz,Topaz,YFP |
物种来源: | A. victoria |
插入大小: | 720 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | 3.6 kb rat type 1 collagen + first intron |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | Sac2(未破坏) |
3' 克隆位点: | Sal1 (未破坏) |
5' 测序引物 | T3 |
3' 测序引物 | T7 |
文献引用方法 |
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材料和方法部分: | pOBCol3.6-GFPtpz was a gift from David Rowe (Addgene plasmid # 110211 ; http://n2t.net/addgene:110211 ; RRID:Addgene_110211) |
参考文献部分: | Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler AC, Rowe D. J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 10.1359/jbmr.2002.17.1.15. 10.1359/jbmr.2002.17.1.15 PubMed 11771662 |
买家导航
pOBCol3.6GFPtpz载体基本信息
pOBCol3.6GFPtpz载体图谱质粒图谱和多克隆位点信息
pOBCol3.6GFPtpz载体简介
pOBCol3.6GFPtpz载体序列
LOCUS pOBCol3.6-GFPtpz 9671 bp ds-DNA circular SYN 07-AUG-2018 DEFINITION Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.. ACCESSION . VERSION . KEYWORDS pOBCol3.6-GFPtpz SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9671) AUTHORS Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler AC, Rowe D TITLE Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. JOURNAL J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 10.1359/jbmr.2002.17.1.15. PUBMED 11771662 REFERENCE 2 (bases 1 to 9671) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9671 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 480..499 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 733..750 /label=L4440 /note="L4440 vector, forward primer" protein_bind 867..888 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 903..933 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 941..957 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 946..968 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 965..981 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 965..981 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 1000..1020 /label=T3 /note="T3 promoter, forward primer" promoter 1002..1020 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 6294..6310 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 6294..6310 /label=pBluescriptSK /note="For pBluescript vector" regulatory 6377..6386 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6383..7102 /codon_start=1 /product="enhanced yellow variant of GFP" /label=Topaz YFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6428..6449) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6689..6708) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7036..7057 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 7139..7363 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(7378..7394) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7379..7395) /label=pBluescriptKS /note="For pBluescript vector" primer_bind complement(7419..7438) /label=T7 /note="T7 promoter, forward primer" promoter complement(7420..7438) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7448..7465) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7448..7464) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7457..7479) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7606..8061 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7693..7712) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7903..7924 /label=F1ori-F /note="F1 origin, forward primer" promoter 8087..8191 /gene="bla" /label=AmpR promoter CDS complement(8680..9339) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 9254..9273 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(9340..9442) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" rep_origin 9662..579 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 61 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 121 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 601 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 661 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 721 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 781 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 841 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 901 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 961 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg 1021 gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaatatagaa gccaaggatt 1081 tcaagggttt ccttttctct cttcttcttt ttttttcttt ttctttttcc tgagatggag 1141 tttccttttg tagccctgac tgtcctggaa ttcactctat agaccaggct agcctcacac 1201 ttagtgatct gcctgcctct gcctcttggg tgctcaggat tcaaggcatg aaccaccact 1261 acccgaccag ggatttctta cacacttctg actggactaa ccaggaaagc agagagggag 1321 acaggaagaa aatgctcaga aggaaggagt aggattggag gtgagctggg ggaacccaga 1381 ctgagccgtg cagaagacaa ggaagaagaa agccacccac acacctagga tccacccaca 1441 gattttgctc tgggtacccc tgtctggaga ctgtagggct ttgtgatgga gggtggggta 1501 tgcttcatgc cccgtgccct ttactccaga cctaaatgcc cacccccaca tacagctgct 1561 cgctctctct ctcccctgcc cttctcccaa gagaccagtt ctccatccct ggtctgcagc 1621 caaggctggg ggcagaagaa ctttctggag gatttgagtg agaaaagcaa gagagcctca 1681 agtagggact ggaacctctg ggaagggagt gcagaggaga cccgggtatg tgccctacct 1741 ggtacattta tacctgggca gcctctgctc ctgttccaga cttcagagcc cagacgggtc 1801 ctctccctcc ctcatgaggg gaaacatttg gggaaatttg gagagagaca gaactcagag 1861 ctcagcactt tcctctttct gtttttcttc ttgaggaatt ttttccccca actgctgatg 1921 actttaccat tcttgggggt gggggggtgg agattctggc ttttgctccc cctacactcc 1981 aagtgccgga caaagcccta cattccacaa gaagccaggg cttcagagtt tcctaaagat 2041 gaggtggcgt ggcgagtctc ctccctctcc cagctccaac tccccctccc ccagtctcca 2101 gccctcagcc tggccaggga ggccccgcca ggctgggagg agaccccaag cacattcttc 2161 ctctcgctgt catgctgcag aaattaaaga cacatctctg agctgggtac ccgccaatcg 2221 tttcaagttg agaagtggca gaggaggtcc cgagcttcag ctccatgcca cgtgtaaagg 2281 aagcttggaa cccactgccc acaactcctg gggcaaaaac ctggagtcag acatggggtg 2341 aaggctgtca cacggcacag acacgtcaag cacccccccc aattctagta gtctcctagc 2401 ctccaccaga accccagacc cttgatgtgg cagtcaccag tccacacctg ttaggctctt 2461 gtctcttctt ccagatgagc ctggggggcg ggggggtgct agatcaggag cagggaaaag 2521 tagctttgga taagtgcttt tcccaataca aaacccaaca aagagtgggc agatcacact 2581 gtgtagtgct tcgtggaacc ctaccctaga caactgcctt gaacacctat tccctctgat 2641 gtacaccatc cccgtccact gttagggagt gggcatcctt tggaactgac cactgtggaa 2701 ggcaggactt tactgagttc cggaactacc atctcagctt ctcagcccca gccttaccct 2761 acaggcactg gcataggcgg gggcagatcc tgggccacaa gtcactgcca catggttggg 2821 ataattgatg aagtcctgtc cttccattgc tgtctccagt tctgcttctc tggaaactct 2881 atatttttcc ctttaattat agcctctgca gtctccctct gccaccccac ccgcaccgct 2941 tagcctaact gcccacggcc agcgacgtgg ctccctcccc ttctgctccc ttggtctttt 3001 ttattttttt ttctttgcct tcgttgcaca aaactagctc agggagggcg tgaagggggg 3061 gggagcaatg gaatcttgga tggtttggag gaggcgggac tccttgcttc cacgtttaca 3121 gctctgaaga cggctgtggg ggaagtgata caggacgtct atgggccctg agaggagacc 3181 cctatgcttc cctgccaccc acacagttta acaaaatgaa gttcctaagt agagtggggg 3241 tcaggcagag cacctttgca gggttgatgg gagcccaggg aaagaaagga cactgtcttt 3301 tagggacaca tttaaatata agccactttt cttgggggac gacaaatgac cctttcctga 3361 ttgcagaggt ggggaacaat ggctgagatt ttcagcaaag aagcgaggac atgaggagta 3421 gccttcaaat aaagtcactc agctaccaaa aacaagtttc tgccacacac cgagttcacc 3481 taggtgtccc cagaccagat ccaagtacag taaggaaagc aggttctcta cagagagaac 3541 acggctctat ggccaatgcc ttctacctgc tctttctgga ttgatactgc tacctaagag 3601 ggcctctaac caattcctgg ctgtagccac agctgacaca agaccttttt ctaagacatc 3661 cctggtcaca ggcctcctgt agcaaattcc agccctggga tggaggtggt caggaaagag 3721 tttatacaag aagacccagg ccacagcttt aaggactcag aaacccccct gcccacacgg 3781 ctgcccatca taacgcagaa ggtttcttct ggaaggacaa ggatgtcaaa cttctcccca 3841 agcctaatcc tcagagatgt ctccctctgt tacacctggg gctggagaaa ggtgggtctt 3901 tcatggagcc acattcatgg cagaacagat agccacccca ctcctttcaa acaaccacat 3961 atctgactct tagtatctgt gaagagatgt ctaatttgtt cccaaatatt cctaccctgc 4021 atacctgggc ccacaccatg aggtattctc ctccctctaa cagtcacatc tgcttagctg 4081 cctggttctt cggatttgga gagatgcttg cctaacttat tcttccttag gtcttcccaa 4141 ggatgccaga aagactatga gacatggcca agaggacctt ttcccaattg tgcctgacac 4201 tgaacccttt gtaatgttcc ccaactcaga ttcccaattc tacatccttc tgatttgagg 4261 tcccagaagg aaagtgcaag gggcatcccc tacccacaat cagtatatcg agggcccagc 4321 cacactcagt gatagcacct ctggcccatg tagatctggg ggacaagggt ggcagaattg 4381 caaagggggg agggggctgg gtggactcct ttcccttcct ttccctcctc ccccctcttc 4441 gttccaaatt gggggccggg ccaggcagtt ctgattggct gggggccggg ctgctggctc 4501 cccctctcca agaggcaggg ttcctcccag ccctcctcca tcaggatggt ataaaagggg 4561 cccaggccag tcgtcggagc agacgggagt ttcacctccg gacggagcag gaggcacacg 4621 gagtgaggcc acgcatgagc cgaagctaac cccccacccc agccgcaaag agtctacatg 4681 tctagggtct agaggcggcc gccttcagct ttgtggacct ccggctcctg ctcctcttag 4741 gggccactgc cctcctgacg catggccaag aagacagtaa gttccaaact ctggggaggt 4801 tatggtttct tgctatcgcg ttctgcatag gggtcctagc aataaagact taaacggaag 4861 cgcggggtgc tccagagtta ggagggatga gaaggtgatg gagaggaact gggaagagag 4921 aggtcaccct cgcgagcaga ggagagtgag cggatctcag aagtggagcc ctggttgaga 4981 tggcacggca ggaaatttca agcatctatc tgtagcgcga cgggaaaagt gggaatctgg 5041 gcaggggttg ggtcagagta cgtcgaagat caaataagac ctctggaata gagattcgaa 5101 gctcaagatg taaaagcaat caaaaaggct gcgggatgat tcataaggaa agattgtttg 5161 ccctctctgc aggctagaca gtgttgctgg ggcctccggg gtgctgggct ctgggagtgg 5221 ggtgggggga ggaagggacg cacggagagg gcgggttgct ggatgagaaa gtttggctaa 5281 gacttgactg ggcgcggtcc ctgtgcctcc tgctggtcaa ccttgagcac tacgagtcct 5341 ggtacagtac tttacagcgc gcgggcttaa gagggaaaac ctgggctatt gccgggcctg 5401 gaccctcatc tccagactag gaaaataaaa gcacagatgg atgtgatagc tttggtcttt 5461 gaaagtgacc caggccagta aaattgggtc cgccccgtgc attgcttagc gttgcccgcc 5521 acctagtggt tgctttgatc cggtgctaga gaggtgggcg tggttaacta atcattgcta 5581 tttgtagact tgagttcttt ctttggctaa ggagtgaccc caaagctcta ctggctttga 5641 aggaaggacc gggcaagctt tgattcctgg gagcggagag ttagggtgta tctaacttta 5701 gggatatatt ttactactct ggacaaggct ccacggaagt caccctcctc cattttagcc 5761 ccgcccattc cgtcccacct gcccaaggaa aggaggtggg accagggctg gaatttccct 5821 tctctcctcc cacccccgcc tccggtctcc ccgcaaacca cgtcccacaa acttccacct 5881 actttgtagg agccctccta ctctccgtca ggaggacttc tatttcattt ggttcccgcg 5941 cgcgtgggct gaagttgagt ttggaagcaa ccccagtcta aagctttgtt taaattcctg 6001 ggaacttgga agtaacagcc ctggccaggc ttcttggtgc tgtcactgaa cgccgttaca 6061 gagcagacct gcatttaagg atttaaggga agaaaatagg ggttggggga agatgtggga 6121 agtggaaaat ctacgtgcac ctcttagaca ggggaaaggg tgagtaaggc tgggatccgg 6181 tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc taggcctgta 6241 cggaagtgtt acttctgctc taaaagctgc tgcaggtctt agttaactaa gactctagaa 6301 ctagtggatc ccccgggctg caggaattcg atatcaagct tatcgataag cttggcattc 6361 cggtactgtt ggtaaagcca ccatggtgag caagggcgag gagctgttca ccggggtggt 6421 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 6481 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 6541 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgc agtgcttcgc 6601 ccgctacccc gaccacatgc gccagcacga cttcttcaag tccgccatgc ccgaaggcta 6661 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 6721 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 6781 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 6841 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 6901 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 6961 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 7021 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 7081 catggacgag ctgtacaagt aactcgctag agtcggggcg gccgggcgag ctcgaattct 7141 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 7201 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 7261 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 7321 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggaattccg agcttatcga 7381 taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg 7441 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 7501 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 7561 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 7621 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 7681 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 7741 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 7801 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 7861 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 7921 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 7981 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 8041 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 8101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 8161 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 8221 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 8281 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 8341 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 8401 ttgatcagaa aaaaaggatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa 8461 actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg 8521 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt 8581 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca 8641 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc 8701 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc 8761 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc 8821 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt 8881 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa 8941 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg 9001 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac 9061 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg 9121 taattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 9181 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 9241 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 9301 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 9361 aaatctcgac aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 9421 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 9481 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 9541 ttattcggtc gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 9601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 9661 cttgagatcc t //
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