phOct4-EGFP

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: phOct4-EGFP 
出品公司: 爱迪基因
目录编号: 38776
存储实验室: Wei Cui

载体骨架基本信息
载体名称: pEGFP-1
载体出品公司: Clontech
载体抗性: 卡那霉素
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: Oct-4 promoter
物种来源: H. sapiens (人)
插入大小: 3950 bp
启动子 human OCT4
标签/融合蛋白: EGFP(C端)
克隆方法: TOPO克隆
5' 测序引物 TT CCC ATG TCA AGT AAG TGG GGT GG
3' 测序引物 CGA GAA GGC AAA ATC TGA AGC CAG G

文献引用方法
材料和方法部分: phOct4-EGFP was a gift from Wei Cui (Addgene plasmid # 38776)
参考文献部分: Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Gerrard L, Zhao D, Clark AJ, Cui W. Stem Cells. 2005;23(1):124-33. 10.1634/stemcells.2004-0102 PubMed 15625129

载体图谱质粒图谱和多克隆位点信息

phOct4-EGFP载体图谱

载体简介

载体序列

LOCUS       phOct4-EGFP	8068 bp 	DNA	SYN
DEFINITION  phOct4-EGFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="phOct4-EGFP"
                     /mol_type="other DNA"
     CDS             complement(1080..1568)
                     /label="ORF frame 3"
     CDS             complement(3017..3487)
                     /label="ORF frame 1"
     CDS             3960..4733
                     /label="ORF frame 3"
     gene            4017..4730
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(4059..4080)
                     /label="EGFP_N_primer"
     misc_feature    4197..4226
                     /label="Y66 (EGFP)"
     misc_feature    4667..4688
                     /label="EGFP_C_primer"
     misc_feature    4947..4966
                     /label="EBV_rev_primer"
     rep_origin      complement(5116..5422)
                     /label="f1_origin"
     promoter        5501..5529
                     /label="AmpR_promoter"
     misc_feature    complement(5595..5615)
                     /label="pBABE_3_primer"
     misc_feature    complement(5601..5816)
                     /label="SV40_enhancer"
     promoter        5613..5881
                     /label="SV40_promoter"
     rep_origin      5780..5857
                     /label="SV40_origin"
     misc_feature    5842..5861
                     /label="SV40pro_F_primer"
     CDS             5964..6758
                     /label="ORF frame 3"
     gene            5967..6755
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(6273..6809)
                     /label="ORF frame 3"
     terminator      6933..7202
                     /label="TK_PA_terminator"
     rep_origin      7350..7969
                     /label="pBR322_origin"
ORIGIN
    1 TAGTTATTAC TAGCGCTACC GGACTCAGAT CTCGAGCTCA AGCTTCCCAT GTCAAGTAAG
   61 TGGGGTGGGT AGATGGGAGG GCATATGAGA GCGGGGCAGC AATTTTATGT CTTAGTTGGA
  121 AGGAAGTTTA GAGTTCATTG AGTCCAAATC CTCTTTACTA GGTGAGGAAA CTGAGGCACA
  181 GGAGGGGGAG GTGCCATTAG CCAGGGAGTC AGAGCCAGGA CTTAGAACCT GGTCCATGCC
  241 TCCTAGTTTG TTCCTGACCA ATCCTCCCAG CTCACCCACT CCTTTTGCTC TCACCTCTGA
  301 CTCCCTACAG GCTACAGCTA GAGAGTTGAC TCCTCTATTT GAGGTAAGAG TGTCTGGATT
  361 TGGGATAAGG TGACCTCAGT TTGCAGGTCT CATGGGGGAG ATCAGAGGGT CCTTTGTTGA
  421 GAAGAGGGGA CTTGGTCCAC AGTGAGCAAA GGAATTTGTC TAAATTGCTA TTAAGGGAGA
  481 TCATCTCCCA AGGTGGGCCA AGCCTGAACC CTCAGCCCTC CATTTCTTCC CTTTCTTTTG
  541 CCTTCAGATT GACAGGCCTC GGAAGTCAAA ATAAGTGGTT TCCTAGACCG GGTCGAGAGC
  601 AAGTCTCTAT TGGTCCCAAC TGAGTTTTTT CAGCTGGTTT TTCAACCAAA CAGCACCTCA
  661 TCTCCCAGTG AGGGGAAGGG AAGGCTGGGC TGAGAGCAGC AAGGCTGCTC ATCTCACCTC
  721 TCCCCACCCA GCCATGCCAG CCGCCACACC TGGTGGGGAG AGGTGGGCCT CACCTGGGTC
  781 CCCTGGCAGT GCTCTGTGAA GGGTCTTGAC ATTGCACTGT AATAATAAAG GTGTGTGTGA
  841 AGTATCTTTT TATGGTGACT TTCTAAAACC CAGGGAATCA TGGGACCAGT TCTGATGACT
  901 CAGCCTGGCT TCCAGTCTCC TCCAGGCCCA ACGGTGGCCC CCAGCACTGG TTGGGGCCTG
  961 GGAGAGCTGG CCTTGGCTGA AGTGAAGCCA CCTACCCTTC AGGCATAACA GGACAGTGAG
 1021 AAGGAAGGAA AGCCTGCCTC AACCTCCCAT CAGCCCTGAG CACCCCAGAA GGGGGCCGGC
 1081 TAGGAGTCTA GGCATGCAGG AGGCTGACCC CTGACTGGGC TCATATCCAG CCACAAGGCA
 1141 GCCAGGGACC CAGGCACCCA CCCCTTGTCT GCGTCCCTCT CGGGAATGGG CCTCTTGCCC
 1201 AGGCCAGAAA TACACCACCT ACAGTACAAA TTATAATCTA AAAACAAGAG GGTGGTGTTG
 1261 AGTGGGGAAA TTGGGGAAGG TGTTTTAGGA GCCACTAGGA AAATGGGCAG CAGGGACTCT
 1321 CTGGACTGGC TTGGGAAGAG CGCTTTTGGG GAACCTGGAG GATGGCAAGC TGAGAAACAC
 1381 TGGTGTGGAG ATTCCAGCCA AATCCCAGGC CTGCCCCTCC CCCTCCTCTG AGAGGCCGTC
 1441 TTCTTGGCAG ACAGCAGAGA GATGCATGAC AAAGGTGCCG TGATGGTTCT GTCCTGGGGA
 1501 TTGAGATGGC TGGGGAGGGG CCTCCTCCTG TTCCGAAGCA TGTTCCTCCC ACCCCCACCA
 1561 GGCCCCATAA TCTACCTGCC TTTTGGGCAG TTAAAGGCCG AGAAGTGAAC ACAGCTGCAA
 1621 CCCCACTGCC TTGTAGACCT TCCGGCAGAC CTGTGGCAGG TATTGAAATG CACGCATACA
 1681 ATTAGGCTCA AAAAGTCTAC ACAGACAGGA GATGGGCACA CGAACAGAGG CAACATAAGA
 1741 GTGGGGGAAA AGTCTCAAAA GACTCACGGA TGCCACCAAG ATGAAGACAG CTGGCCACGG
 1801 GACACCCATC CCCTTAGAAG GCAGACAGAG CCACTGACCC CAGCAGACAA GCCCAGGCAG
 1861 GGCTGAGCCT GGAGCCTGCA ATGAGAAGCC TTACTTAAGT CGACAGAGGT CAGCGTGCCC
 1921 AGTCCAGACC TGGCCTTCTG GCCTTCGAAG CTGTGGGGAG CCCTGGCCCA GAGCCCCCTC
 1981 TGGAGCCCCC AGACTTACCC CAGGCCCTCC ACTGAGATCA AGTTTTGGGA GCAGACAGAC
 2041 AAACATCATC CCTCACAGAC AGGCATTCCG TTGGCTATTC TCTTGCAAAC AGAATCAAGC
 2101 ACTAGACCAG CAGCATGAGC CTCAGGATAC TCAGGCCAGG CCCAGAAAAA CAGACCCTGA
 2161 AGGGGAGCTT AGGGCAGCCT TCCTGCACCC CTCCACAAAT CACTCGCCAC CTCCTCTGCG
 2221 TCTTTCTGCC AGCCAGCCCC ACTAAACAAA GCACATCCCT CAATCTTCCG GGCTCGGGGA
 2281 GGGATGCACG ATGAAGCTGG ACGCCTGAGT CCCCCAGAGG AAGGAGGAAC TAGATACCTA
 2341 GGTCCCTGTA GGGGGCCCTT GGTGCCCGTC TGAGGCTCAG TCTTTGAGGG GATTGCAGAG
 2401 GGGGGTTGCT GGAGCTCCTT TTAGCGTCTC TGAAGGGGAT TCTGTGTGAG GGGATTGGGA
 2461 CTGGGGGGTT GGGGAGCAGG AAGCAGTCCC CAGGGGAGCC ATCCAGGCCC ATTCAAGGGT
 2521 TGAGCACTTG TTTAGGGTTA GAGCTGCCCC CTCTGGGGAC CGGGATTGTC CAGCCAAGGC
 2581 CATTGTCCTG CCCCCTTCCC CCAGTCCCTC CCAGGCTTCT TTGAACCTGA AGTCAGATAT
 2641 TTTTTCTCCA CACCCCCCAC CCCCTGGTTT TCCCCACCCA GGGCCTAGGG CTGGAGGCCT
 2701 GGGCCAGGGA GGTGGGGGAG GGAGAACGGG GCCTACCGTG GTATTAGATG TCTGAGTTTT
 2761 GGTTGAGAGG GGAGCAAGGA ACCTGATGTG CAGGTTCCAT AGTGGAGGGG GCCCAAAGCG
 2821 GGTGTCTTAT CACTCTGTTT CAGCAAAGGT TGGGAAACTG AGGCCCAATC AGTCCAAAGT
 2881 CTGGTCCCTT GAAGGGGAAG TAGGGGCCAA CCCCTTAGTC TGTTAGATGA GGAGAGTCTG
 2941 GAGTCTGATT CTGGAAGACG GAGGGGTGGG GGGATGGGGG GTGGGGGGAT ATAGCACGGA
 3001 GGCCTTGTCT GGCAGTCTAC TCTTGAAGAT GGGGTGAAAT TTGGCAGGCT GGGCAGATGG
 3061 TGCCAGGCAC CCAGGCTGCG GGGTGGCTGG ATTTGGCCAG TATCGGGATG GGAATGCCTA
 3121 GGATTCTGGA TGGATCGGGG GAAGGCATAA GGGAGCAGCT GGCCATTGTG CTTATGGCTG
 3181 TTGATGCATT GAGGGATAGC GCCACACACA CATTCAATAA ATTTGAGGAG CTGAGAGGGT
 3241 GACTGGCCCC TGAAGGCACA GTGCCAGAGG TCTGTGGAGA GGGGGTCAAG CACCTGGGTT
 3301 CCTGAAGAAC ATGGAGGTGT GGGAGTGATT CCAGACAGCT GGGATGTGCA GAGCCTGAGA
 3361 GAGTGCCAGG GAGCGGGTTG GGAGTTGAAA GTTGGGTGTG GTGGCTCACG CCTTTAATCA
 3421 TGACACTGGG CGGCAGAGGC GGGAGGATTT CTTGAGGACA GGAATTCAAG ACCAGCCTGG
 3481 GTAACATAGC AAGGCCCCAT CTCTACTAAA AATAAAAAAA CTAACAGGGC ACAGTGGTCC
 3541 AAGCCTGTAG TCCCAGCCAC TTAGGAGGCT GGAGCAGAAG GATTGCTTTG GCCCAGTAGA
 3601 TCGAGGCTAC ATTGAGCCAT CATTGTACTC CACTGCACTC CAGTCTGGGC AACAAAGTGA
 3661 GACCCTGTCT TAAAAAATAA AAATAAAAAA AGTTTCTGTG GGGGACCTGC ACTGAGGTCC
 3721 TGGAGGGGCG CCAGTTGTGT CTCCCGGTTT TCCCCTTCCA CAGACACCAT TGCCACCACC
 3781 ATTAGGCAAA CATCCTTCGC CTCAGTTTCT CCCCCCACCT CCCTCTCCTC CACCCATCCA
 3841 GGGGGCGGGG CCAGAGGTCA AGGCTAGTGG GTGGGACTGG GGAGGGAGAG AGGGGTTGAG
 3901 TAGTCCCTTC GCAAGCCCTC ATTTCACCAG GCCCCCGGCT TGGGGCGCCT TCCTTCCCCA
 3961 TGGCGGGACA CCTGGCTTCA GATTTTGCCT TCTCGGATCC ACCGGTCGCC ACCATGGTGA
 4021 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
 4081 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
 4141 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
 4201 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
 4261 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
 4321 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
 4381 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
 4441 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
 4501 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
 4561 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
 4621 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
 4681 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC
 4741 GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA
 4801 CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT
 4861 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
 4921 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAGG
 4981 CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT
 5041 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG
 5101 AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT
 5161 CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC
 5221 CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA
 5281 GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA
 5341 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA
 5401 CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA
 5461 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
 5521 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG
 5581 AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC
 5641 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC
 5701 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT
 5761 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC
 5821 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT
 5881 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG
 5941 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG
 6001 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG
 6061 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC
 6121 TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA
 6181 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC
 6241 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG
 6301 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT
 6361 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG
 6421 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA
 6481 GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT
 6541 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG
 6601 GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG
 6661 GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC
 6721 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT
 6781 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA
 6841 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG
 6901 GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA
 6961 GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG
 7021 TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC
 7081 CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC
 7141 CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT
 7201 AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
 7261 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
 7321 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
 7381 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
 7441 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
 7501 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
 7561 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
 7621 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
 7681 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
 7741 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
 7801 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
 7861 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
 7921 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
 7981 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
 8041 TGTGGATAAC CGTATTACCG CCATGCAT
//

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