质粒名称: | phOct4-EGFP |
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出品公司: | 爱迪基因 |
目录编号: | 38776 |
存储实验室: | Wei Cui |
载体骨架基本信息 |
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载体名称: | pEGFP-1 |
载体出品公司: | Clontech |
载体抗性: | 卡那霉素 |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Oct-4 promoter |
物种来源: | H. sapiens (人) |
插入大小: | 3950 bp |
启动子 | human OCT4 |
标签/融合蛋白: | EGFP(C端) |
克隆方法: | TOPO克隆 |
5' 测序引物 | TT CCC ATG TCA AGT AAG TGG GGT GG |
3' 测序引物 | CGA GAA GGC AAA ATC TGA AGC CAG G |
文献引用方法 |
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材料和方法部分: | phOct4-EGFP was a gift from Wei Cui (Addgene plasmid # 38776) |
参考文献部分: | Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Gerrard L, Zhao D, Clark AJ, Cui W. Stem Cells. 2005;23(1):124-33. 10.1634/stemcells.2004-0102 PubMed 15625129 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS phOct4-EGFP 8068 bp DNA SYN DEFINITION phOct4-EGFP ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8068 /organism="phOct4-EGFP" /mol_type="other DNA" CDS complement(1080..1568) /label="ORF frame 3" CDS complement(3017..3487) /label="ORF frame 1" CDS 3960..4733 /label="ORF frame 3" gene 4017..4730 /label="EGFP" /gene="EGFP" misc_feature complement(4059..4080) /label="EGFP_N_primer" misc_feature 4197..4226 /label="Y66 (EGFP)" misc_feature 4667..4688 /label="EGFP_C_primer" misc_feature 4947..4966 /label="EBV_rev_primer" rep_origin complement(5116..5422) /label="f1_origin" promoter 5501..5529 /label="AmpR_promoter" misc_feature complement(5595..5615) /label="pBABE_3_primer" misc_feature complement(5601..5816) /label="SV40_enhancer" promoter 5613..5881 /label="SV40_promoter" rep_origin 5780..5857 /label="SV40_origin" misc_feature 5842..5861 /label="SV40pro_F_primer" CDS 5964..6758 /label="ORF frame 3" gene 5967..6755 /label="NeoR/KanR" /gene="NeoR/KanR" CDS complement(6273..6809) /label="ORF frame 3" terminator 6933..7202 /label="TK_PA_terminator" rep_origin 7350..7969 /label="pBR322_origin" ORIGIN 1 TAGTTATTAC TAGCGCTACC GGACTCAGAT CTCGAGCTCA AGCTTCCCAT GTCAAGTAAG 61 TGGGGTGGGT AGATGGGAGG GCATATGAGA GCGGGGCAGC AATTTTATGT CTTAGTTGGA 121 AGGAAGTTTA GAGTTCATTG AGTCCAAATC CTCTTTACTA GGTGAGGAAA CTGAGGCACA 181 GGAGGGGGAG GTGCCATTAG CCAGGGAGTC AGAGCCAGGA CTTAGAACCT GGTCCATGCC 241 TCCTAGTTTG TTCCTGACCA ATCCTCCCAG CTCACCCACT CCTTTTGCTC TCACCTCTGA 301 CTCCCTACAG GCTACAGCTA GAGAGTTGAC TCCTCTATTT GAGGTAAGAG TGTCTGGATT 361 TGGGATAAGG TGACCTCAGT TTGCAGGTCT CATGGGGGAG ATCAGAGGGT CCTTTGTTGA 421 GAAGAGGGGA CTTGGTCCAC AGTGAGCAAA GGAATTTGTC TAAATTGCTA TTAAGGGAGA 481 TCATCTCCCA AGGTGGGCCA AGCCTGAACC CTCAGCCCTC CATTTCTTCC CTTTCTTTTG 541 CCTTCAGATT GACAGGCCTC GGAAGTCAAA ATAAGTGGTT TCCTAGACCG GGTCGAGAGC 601 AAGTCTCTAT TGGTCCCAAC TGAGTTTTTT CAGCTGGTTT TTCAACCAAA CAGCACCTCA 661 TCTCCCAGTG AGGGGAAGGG AAGGCTGGGC TGAGAGCAGC AAGGCTGCTC ATCTCACCTC 721 TCCCCACCCA GCCATGCCAG CCGCCACACC TGGTGGGGAG AGGTGGGCCT CACCTGGGTC 781 CCCTGGCAGT GCTCTGTGAA GGGTCTTGAC ATTGCACTGT AATAATAAAG GTGTGTGTGA 841 AGTATCTTTT TATGGTGACT TTCTAAAACC CAGGGAATCA TGGGACCAGT TCTGATGACT 901 CAGCCTGGCT TCCAGTCTCC TCCAGGCCCA ACGGTGGCCC CCAGCACTGG TTGGGGCCTG 961 GGAGAGCTGG CCTTGGCTGA AGTGAAGCCA CCTACCCTTC AGGCATAACA GGACAGTGAG 1021 AAGGAAGGAA AGCCTGCCTC AACCTCCCAT CAGCCCTGAG CACCCCAGAA GGGGGCCGGC 1081 TAGGAGTCTA GGCATGCAGG AGGCTGACCC CTGACTGGGC TCATATCCAG CCACAAGGCA 1141 GCCAGGGACC CAGGCACCCA CCCCTTGTCT GCGTCCCTCT CGGGAATGGG CCTCTTGCCC 1201 AGGCCAGAAA TACACCACCT ACAGTACAAA TTATAATCTA AAAACAAGAG GGTGGTGTTG 1261 AGTGGGGAAA TTGGGGAAGG TGTTTTAGGA GCCACTAGGA AAATGGGCAG CAGGGACTCT 1321 CTGGACTGGC TTGGGAAGAG CGCTTTTGGG GAACCTGGAG GATGGCAAGC TGAGAAACAC 1381 TGGTGTGGAG ATTCCAGCCA AATCCCAGGC CTGCCCCTCC CCCTCCTCTG AGAGGCCGTC 1441 TTCTTGGCAG ACAGCAGAGA GATGCATGAC AAAGGTGCCG TGATGGTTCT GTCCTGGGGA 1501 TTGAGATGGC TGGGGAGGGG CCTCCTCCTG TTCCGAAGCA TGTTCCTCCC ACCCCCACCA 1561 GGCCCCATAA TCTACCTGCC TTTTGGGCAG TTAAAGGCCG AGAAGTGAAC ACAGCTGCAA 1621 CCCCACTGCC TTGTAGACCT TCCGGCAGAC CTGTGGCAGG TATTGAAATG CACGCATACA 1681 ATTAGGCTCA AAAAGTCTAC ACAGACAGGA GATGGGCACA CGAACAGAGG CAACATAAGA 1741 GTGGGGGAAA AGTCTCAAAA GACTCACGGA TGCCACCAAG ATGAAGACAG CTGGCCACGG 1801 GACACCCATC CCCTTAGAAG GCAGACAGAG CCACTGACCC CAGCAGACAA GCCCAGGCAG 1861 GGCTGAGCCT GGAGCCTGCA ATGAGAAGCC TTACTTAAGT CGACAGAGGT CAGCGTGCCC 1921 AGTCCAGACC TGGCCTTCTG GCCTTCGAAG CTGTGGGGAG CCCTGGCCCA GAGCCCCCTC 1981 TGGAGCCCCC AGACTTACCC CAGGCCCTCC ACTGAGATCA AGTTTTGGGA GCAGACAGAC 2041 AAACATCATC CCTCACAGAC AGGCATTCCG TTGGCTATTC TCTTGCAAAC AGAATCAAGC 2101 ACTAGACCAG CAGCATGAGC CTCAGGATAC TCAGGCCAGG CCCAGAAAAA CAGACCCTGA 2161 AGGGGAGCTT AGGGCAGCCT TCCTGCACCC CTCCACAAAT CACTCGCCAC CTCCTCTGCG 2221 TCTTTCTGCC AGCCAGCCCC ACTAAACAAA GCACATCCCT CAATCTTCCG GGCTCGGGGA 2281 GGGATGCACG ATGAAGCTGG ACGCCTGAGT CCCCCAGAGG AAGGAGGAAC TAGATACCTA 2341 GGTCCCTGTA GGGGGCCCTT GGTGCCCGTC TGAGGCTCAG TCTTTGAGGG GATTGCAGAG 2401 GGGGGTTGCT GGAGCTCCTT TTAGCGTCTC TGAAGGGGAT TCTGTGTGAG GGGATTGGGA 2461 CTGGGGGGTT GGGGAGCAGG AAGCAGTCCC CAGGGGAGCC ATCCAGGCCC ATTCAAGGGT 2521 TGAGCACTTG TTTAGGGTTA GAGCTGCCCC CTCTGGGGAC CGGGATTGTC CAGCCAAGGC 2581 CATTGTCCTG CCCCCTTCCC CCAGTCCCTC CCAGGCTTCT TTGAACCTGA AGTCAGATAT 2641 TTTTTCTCCA CACCCCCCAC CCCCTGGTTT TCCCCACCCA GGGCCTAGGG CTGGAGGCCT 2701 GGGCCAGGGA GGTGGGGGAG GGAGAACGGG GCCTACCGTG GTATTAGATG TCTGAGTTTT 2761 GGTTGAGAGG GGAGCAAGGA ACCTGATGTG CAGGTTCCAT AGTGGAGGGG GCCCAAAGCG 2821 GGTGTCTTAT CACTCTGTTT CAGCAAAGGT TGGGAAACTG AGGCCCAATC AGTCCAAAGT 2881 CTGGTCCCTT GAAGGGGAAG TAGGGGCCAA CCCCTTAGTC TGTTAGATGA GGAGAGTCTG 2941 GAGTCTGATT CTGGAAGACG GAGGGGTGGG GGGATGGGGG GTGGGGGGAT ATAGCACGGA 3001 GGCCTTGTCT GGCAGTCTAC TCTTGAAGAT GGGGTGAAAT TTGGCAGGCT GGGCAGATGG 3061 TGCCAGGCAC CCAGGCTGCG GGGTGGCTGG ATTTGGCCAG TATCGGGATG GGAATGCCTA 3121 GGATTCTGGA TGGATCGGGG GAAGGCATAA GGGAGCAGCT GGCCATTGTG CTTATGGCTG 3181 TTGATGCATT GAGGGATAGC GCCACACACA CATTCAATAA ATTTGAGGAG CTGAGAGGGT 3241 GACTGGCCCC TGAAGGCACA GTGCCAGAGG TCTGTGGAGA GGGGGTCAAG CACCTGGGTT 3301 CCTGAAGAAC ATGGAGGTGT GGGAGTGATT CCAGACAGCT GGGATGTGCA GAGCCTGAGA 3361 GAGTGCCAGG GAGCGGGTTG GGAGTTGAAA GTTGGGTGTG GTGGCTCACG CCTTTAATCA 3421 TGACACTGGG CGGCAGAGGC GGGAGGATTT CTTGAGGACA GGAATTCAAG ACCAGCCTGG 3481 GTAACATAGC AAGGCCCCAT CTCTACTAAA AATAAAAAAA CTAACAGGGC ACAGTGGTCC 3541 AAGCCTGTAG TCCCAGCCAC TTAGGAGGCT GGAGCAGAAG GATTGCTTTG GCCCAGTAGA 3601 TCGAGGCTAC ATTGAGCCAT CATTGTACTC CACTGCACTC CAGTCTGGGC AACAAAGTGA 3661 GACCCTGTCT TAAAAAATAA AAATAAAAAA AGTTTCTGTG GGGGACCTGC ACTGAGGTCC 3721 TGGAGGGGCG CCAGTTGTGT CTCCCGGTTT TCCCCTTCCA CAGACACCAT TGCCACCACC 3781 ATTAGGCAAA CATCCTTCGC CTCAGTTTCT CCCCCCACCT CCCTCTCCTC CACCCATCCA 3841 GGGGGCGGGG CCAGAGGTCA AGGCTAGTGG GTGGGACTGG GGAGGGAGAG AGGGGTTGAG 3901 TAGTCCCTTC GCAAGCCCTC ATTTCACCAG GCCCCCGGCT TGGGGCGCCT TCCTTCCCCA 3961 TGGCGGGACA CCTGGCTTCA GATTTTGCCT TCTCGGATCC ACCGGTCGCC ACCATGGTGA 4021 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG 4081 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC 4141 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA 4201 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG 4261 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG 4321 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC 4381 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG 4441 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA 4501 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT 4561 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA 4621 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG 4681 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC 4741 GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA 4801 CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT 4861 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 4921 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAGG 4981 CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT 5041 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG 5101 AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT 5161 CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC 5221 CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA 5281 GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA 5341 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA 5401 CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA 5461 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA 5521 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG 5581 AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC 5641 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC 5701 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT 5761 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC 5821 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT 5881 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG 5941 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG 6001 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG 6061 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 6121 TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA 6181 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC 6241 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG 6301 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT 6361 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG 6421 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA 6481 GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT 6541 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG 6601 GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG 6661 GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC 6721 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT 6781 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA 6841 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG 6901 GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA 6961 GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG 7021 TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC 7081 CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC 7141 CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT 7201 AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG 7261 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC 7321 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 7381 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 7441 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 7501 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 7561 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 7621 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 7681 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 7741 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 7801 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 7861 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 7921 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 7981 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC 8041 TGTGGATAAC CGTATTACCG CCATGCAT //
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