质粒名称: | pLOX-CWBmi1 |
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出品公司: | 爱迪基因 |
目录编号: | 12240 |
存储实验室: | Didier Trono |
载体骨架基本信息 |
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载体名称: | pHR |
载体抗性: | 氨苄霉素 |
空载体大小: | 9967 bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Bmi1 |
物种来源: | M. musculus (鼠) |
基因突变: | CMV promoter upstream of mBmi-1. LoxP in 3' LTR. |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | |
3' 克隆位点: | |
5' 测序引物 | CMV-F |
3' 测序引物 | SP6 for LoxP site |
文献引用方法 |
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材料和方法部分: | pLOX-CWBmi1 was a gift from Didier Trono (Addgene plasmid # 12240) |
参考文献部分: | Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D. Mol Ther. 2000 Oct . 2(4):404-14. 10.1006/mthe.2000.0141 PubMed 11020357 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLOX-CWBmi1 9967 bp DNA SYN DEFINITION pLOX-CWBmi1 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..9967 /organism="pLOX-CWBmi1" /mol_type="other DNA" misc_feature 457..637 /label="HIV-1_5_LTR" misc_feature 457..637 /label="truncHIV-1_3_LTR" misc_feature 748..792 /label="HIV-1_psi_pack" CDS 1180..2028 /label="ORF frame 1" misc_feature 1302..1535 /label="RRE" promoter 2040..2544 /label="CMV_immearly_promoter" misc_feature 2047..2334 /label="CAG_enhancer" misc_feature 2501..2521 /label="CMV_fwd_primer" promoter 2502..2571 /label="CMV_promoter" misc_feature 2545..2564 /label="pCEP_fwd_primer" misc_feature 2547..2571 /label="LNCX_primer" CDS 2681..3655 /label="ORF frame 2" misc_feature 3801..4393 /label="WPRE" CDS complement(3886..4389) /label="ORF frame 2" CDS 3889..4449 /label="ORF frame 1" misc_feature 4459..4474 /label="cPPT" misc_feature 4459..4480 /label="U3PPT" misc_feature 4531..4564 /label="loxP" misc_feature 4584..4764 /label="HIV-1_5_LTR" misc_feature 4584..4764 /label="truncHIV-1_3_LTR" misc_feature complement(4790..4807) /label="Sp6_primer" gene 5045..5308 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(5097..5116) /label="pBRrevBam_primer" misc_feature 5449..5471 /label="pGEX_3_primer" promoter 5630..5658 /label="AmpR_promoter" gene 5700..6560 /label="Ampicillin" /gene="Ampicillin" CDS 5700..6560 /label="ORF frame 3" rep_origin 6715..7334 /label="pBR322_origin" misc_feature complement(7560..7580) /label="pBABE_3_primer" misc_feature complement(7566..8019) /label="SV40_enhancer" promoter 7578..7846 /label="SV40_promoter" rep_origin 7745..7822 /label="SV40_origin" misc_feature 7807..7826 /label="SV40pro_F_primer" misc_feature 9027..9042 /label="SV40_int" misc_feature 9048..9095 /label="SV40_3_splice" CDS 9172..9645 /label="ORF frame 1" misc_feature 9223..9243 /label="SV40 NLS" misc_feature 9229..9243 /label="NLS" terminator 9671..9802 /label="SV40_PA_terminator" misc_feature 9759..9778 /label="EBV_rev_primer" ORIGIN 1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc 61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc 121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg 181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc 241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc 301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt 361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca 421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc 481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga 661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca 721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct 781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat 841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat 901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag 961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta 1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag 1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac 1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt 1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca 1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg 1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc 1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg 1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg 1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc 2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg 2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga 2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatctgata 2641 tcatcgatga attctttccg ggatctttta tcaagcagaa atgcatcgaa caaccagaat 2701 caagatcact gagctaaatc cccacttaat gtgtgtcctg tgtggagggt acttcattga 2761 tgccactacc ataatagaat gtctacattc cttctgtaaa acatgtattg tacgttactt 2821 ggagaccagc aagtattgtc ctatttgtga tgtccaggtt cacaaaacca gaccactcct 2881 gaacataagg tcagataaaa ctcttcaaga tattgtatac aaattagtcc cagggctttt 2941 caaaaatgag atgaagagaa gaagagattt ttatgcagct cacccgtcag ctgatgctgc 3001 caatggctcc aatgaagacc gaggagaagt tgcagatgag gagaagagga ttataactga 3061 tgatgagata ataagcttgt ctattgagtt ctttgatcag agcagattgg atcggaaagt 3121 aaataaagag aagcctaagg aagaggtgaa tgataaaagg tacttacgat gcccagcagc 3181 aatgactgtg atgcacttga gaaagtttct caggagtaaa atggacatac ccaatacttt 3241 ccagattgac gtcatgtatg aagaggaacc tttaaaggat tactacacgc taatggacat 3301 tgcctacatt tatacctgga gaagaaatgg cccactacct ttgaaataca gagttcggcc 3361 aacttgcaaa agaatgaaga tgagtcacca gagggatgga ctgacgaatg ctggagagct 3421 ggaaagtgac tctgggagtg acaaggccaa cagcccagcc ggaggtgttc cctccacctc 3481 ttcctgtttg cccagtccca gcactccagt gcagtctcct cacccacagt tccctcacat 3541 ttccagtacc atgaatggaa ccagcaacag ccccagtgct aaccaccaat cttcctttgc 3601 cagtagacct cgaaaatcat cactaaatgg gtcatcagca acttcatctg gttaggactg 3661 ttaaggaaaa gatttttcaa ccccctgatt tagttacctt cattcattac agctttatag 3721 atgcttaata catgtgactg tcgtccagtt tgcttccttt tgtagtgact ttaaatttct 3781 gctcgaggga attccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt 3841 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 3901 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 3961 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 4021 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 4081 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 4141 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 4201 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 4261 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 4321 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 4381 tccccgcatc gggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt 4441 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 4501 aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca ttatacgaag 4561 ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 4621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 4681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 4741 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca 4801 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga 4861 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct 4921 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc 4981 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct 5041 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 5101 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg 5161 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt 5221 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt 5281 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct 5341 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac 5401 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 5461 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac 5521 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 5581 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 5641 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 5701 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 5761 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 5821 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 5881 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 5941 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 6001 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 6061 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 6121 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 6181 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 6241 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 6301 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 6361 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 6421 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 6481 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 6541 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 6601 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 6661 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 6721 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 6781 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 6841 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 6901 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 6961 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 7021 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 7081 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc 7141 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 7201 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 7261 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 7321 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 7381 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 7441 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 7501 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg 7561 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 7621 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 7681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 7741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 7801 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 7861 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg 7921 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg 7981 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta 8041 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg 8101 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac 8161 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg 8221 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta 8281 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg 8341 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat 8401 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga 8461 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt 8521 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt 8581 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat 8641 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa 8701 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg 8761 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg 8821 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac 8881 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg 8941 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa 9001 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt 9061 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg 9121 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct 9181 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag 9241 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt 9301 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta 9361 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat 9421 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat 9481 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg 9541 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact 9601 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 9661 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 9721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 9781 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact 9841 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct 9901 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa 9961 acttagt //