| 质粒名称: | pHAGE2-TetOminiCMV-SKM |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 136551 |
| 存储实验室: | Hans Schöler |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE2 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6000 bp |
| 完整质粒大小: | 10186 bp |
| 载体类型: | 细菌表达，慢病毒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Sox2-T2A-Klf4-E2A-cMyc |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 4000 bp |
| 启动子 | tetO-miniCMV（dox诱导型） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI (未破坏) |
| 3' 克隆位点: | ClaI (未破坏) |
| 5' 测序引物 | GAGACGCCATCCACGCTGT |
| 3' 测序引物 | AGGAAGGTCCGCTGGATTGA |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE2-TetOminiCMV-SKM was a gift from Hans Schöler (Addgene plasmid # 136551 ; http://n2t.net/addgene:136551 ; RRID:Addgene_136551) |
| 参考文献部分: | Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR. Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 10.1016/j.stem.2019.10.002. 10.1016/j.stem.2019.10.002 PubMed 31708402 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE2-TetOminiCMV-SKM 10186 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Dox-inducible polycistronic reprogramming lentiviral vector
expressing mouse Sox2, Klf4, cMyc.
ACCESSION .
VERSION .
KEYWORDS pHAGE2-TetOminiCMV-SKM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10186)
AUTHORS Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
TITLE Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental
Potential of iPSCs.
JOURNAL Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi:
10.1016/j.stem.2019.10.002.
PUBMED 31708402
REFERENCE 2 (bases 1 to 10186)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10186
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 77..101
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 1145..1198
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 2669..2728
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
misc_feature 4116..4704
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4169..4189)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4587..4598)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4852..5032
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5079..5097)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5165..5269
/gene="bla"
/label=AmpR promoter
CDS 5270..6130
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5488..5507)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6301..6889
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6790..6809
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7043..7060
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 7152..7171
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 7300..7321
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7336..7366
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7374..7390
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7379..7401
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 7398..7414
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7398..7414
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 7464..7483
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 7675..8308
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 8355..8480
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8977..9210
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9395..9439
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 9732..9849
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 9925..9
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
ORIGIN
1 tgatagagaa aagtgaaagt cgagctcggt acccgggtcg aggtaggcat gtacggtggg
61 aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
121 gctgttttga cctccataga agacaccggg accgatccag cctgcggccg ccatgtataa
181 catgatggag acggagctga agccgccggg cccgcagcaa gcttcggggg gcggcggcgg
241 aggaggcaac gccacggcgg cggcaaccgg cggcaaccag aagaacagcc cggaccgcgt
301 caagaggccc atgaacgcct tcatggtatg gtcccggggg cagcggcgta agatggccca
361 ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgcgg agtggaaact
421 tttgtccgag accgagaagc ggccgttcat cgacgaggcc aagcggctgc gcgctctgca
481 catgaaggag cacccggatt ataaataccg gccgcggcgg aaaaccaaga cgctcatgaa
541 gaaggataag tacacgcttc ccggaggctt gctggccccc ggcgggaaca gcatggcgag
601 cggggttggg gtgggcgccg gcctgggtgc gggcgtgaac cagcgcatgg acagctacgc
661 gcacatgaac ggctggagca acggcagcta cagcatgatg caggagcagc tgggctaccc
721 gcagcacccg ggcctcaacg ctcacggcgc ggcacagatg caaccgatgc accgctacga
781 cgtcagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc
841 cacctacagc atgtcctact cgcagcaggg cacccccggt atggcgctgg gctccatggg
901 ctctgtggtc aagtccgagg ccagctccag cccccccgtg gttacctctt cctcccactc
961 cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtacc tccccggcgc
1021 cgaggtgccg gagcccgctg cgcccagtag actgcacatg gcccagcact accagagcgg
1081 cccggtgccc ggcacggcca ttaacggcac actgcccctg tcgcacatgg catgcggctc
1141 cggcgagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccggcccact
1201 cgagatgagg cagccacctg gcgagtctga catggctgtc agcgacgctc tgctcccgtc
1261 cttctccacg ttcgcgtccg gcccggcggg aagggagaag acactgcgtc cagcaggtgc
1321 cccgactaac cgttggcgtg aggaactctc tcacatgaag cgacttcccc cacttcccgg
1381 ccgcccctac gacctggcgg cgacggtggc cacagacctg gagagtggcg gagctggtgc
1441 agcttgcagc agtaacaacc cggccctcct agcccggagg gagaccgagg agttcaacga
1501 cctcctggac ctagacttta tcctttccaa ctcgctaacc caccaggaat cggtggccgc
1561 caccgtgacc acctcggcgt cagcttcatc ctcgtcttcc ccagcgagca gcggccctgc
1621 cagcgcgccc tccacctgca gcttcagcta tccgatccgg gccgggggtg acccgggcgt
1681 ggctgccagc aacacaggtg gagggctcct ctacagccga gaatctgcgc cacctcccac
1741 ggcccccttc aacctggcgg acatcaatga cgtgagcccc tcgggcggct tcgtggctga
1801 gctcctgcgg ccggagttgg acccagtata cattccgcca cagcagcctc agccgccagg
1861 tggcgggctg atgggcaagt ttgtgctgaa ggcgtctctg accacccctg gcagcgagta
1921 cagcagccct tcggtcatca gtgttagcaa aggaagccca gacggcagcc accccgtggt
1981 agtggcgccc tacagcggtg gcccgccgcg catgtgcccc aagattaagc aagaggcggt
2041 cccgtcctgc acggtcagcc ggtccctaga ggcccatttg agcgctggac cccagctcag
2101 caacggccac cggcccaaca cacacgactt ccccctgggg cggcagctcc ccaccaggac
2161 tacccctaca ctgagtcccg aggaactgct gaacagcagg gactgtcacc ctggcctgcc
2221 tcttccccca ggattccatc cccatccggg gcccaactac cctcctttcc tgccagacca
2281 gatgcagtca caagtcccct ctctccatta tcaagagctc atgccaccgg gttcctgcct
2341 gccagaggag cccaagccaa agaggggaag aaggtcgtgg ccccggaaaa gaacagccac
2401 ccacacttgt gactatgcag gctgtggcaa aacctatacc aagagttctc atctcaaggc
2461 acacctgcga actcacacag gcgagaaacc ttaccactgt gactgggacg gctgtgggtg
2521 gaaattcgcc cgctccgatg aactgaccag gcactaccgc aaacacacag ggcaccggcc
2581 ctttcagtgc cagaagtgcg acagggcctt ttccaggtcg gaccaccttg ccttacacat
2641 gaagaggcac tttctcgagg gctcgggcca gtgtactaat tatgctctct tgaaattggc
2701 tggagatgtt gagagcaacc caggtcccgg cgcgccactg gatttccttt gggcgttgga
2761 aaccccgcag acagccacga cgatgcccct caacgtgaac ttcaccaaca ggaactatga
2821 cctcgactac gactccgtac agccctattt catctgcgac gaggaagaga atttctatca
2881 ccagcaacag cagagcgagc tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt
2941 cgagctgctt cccaccccgc ccctgtcccc gagccgccgc tccgggctct gctctccatc
3001 ctatgttgcg gtcgctacgt ccttctcccc aagggaagac gatgacggcg gcggtggcaa
3061 cttctccacc gccgatcagc tggagatgat gaccgagtta cttggaggag acatggtgaa
3121 ccagagcttc atctgcgatc ctgacgacga gaccttcatc aagaacatca tcatccagga
3181 ctgtatgtgg agcggtttct cagccgctgc caagctggtc tcggagaagc tggcctccta
3241 ccaggctgcg cgcaaagaca gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc
3301 cacctccagc ctgtacctgc aggacctcac cgccgccgcg tccgagtgca ttgacccctc
3361 agtggtcttt ccctacccgc tcaacgacag cagctcgccc aaatcctgta cctcgtccga
3421 ttccacggcc ttctctcctt cctcggactc gctgctgtcc tccgagtcct ccccacgggc
3481 cagccctgag cccctagtgc tgcatgagga gacaccgccc accaccagca gcgactctga
3541 agaagagcaa gaagatgagg aagaaattga tgtggtgtct gtggagaaga ggcaaacccc
3601 tgccaagagg tcggagtcgg gctcatctcc atcccgaggc cacagcaaac ctccgcacag
3661 cccactggtc ctcaagaggt gccacgtctc cactcaccag cacaactacg ccgcaccccc
3721 ctccacaagg aaggactatc cagctgccaa gagggccaag ttggacagtg gcagggtcct
3781 gaagcagatc agcaacaacc gcaagtgctc cagccccagg tcctcagaca cggaggaaaa
3841 cgacaagagg cggacacaca acgtcttgga acgtcagagg aggaacgagc tgaagcgcag
3901 cttttttgcc ctgcgtgacc agatccctga attggaaaac aacgaaaagg cccccaaggt
3961 agtgatcctc aaaaaagcca ccgcctacat cctgtccatt caagcagacg agcacaagct
4021 cacctctgaa aaggacttat tgaggaaacg acgagaacag ttgaaacaca aactcgaaca
4081 gcttcgaaac tctggtgcat aaatcgatag atcctaatca acctctggat tacaaaattt
4141 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
4201 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
4261 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
4321 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
4381 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
4441 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
4501 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
4561 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
4621 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
4681 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
4741 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
4801 cgaagacaag atcacctgca ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc
4861 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
4921 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
4981 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cacccgggcg
5041 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
5101 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
5161 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
5221 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
5281 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
5341 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
5401 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
5461 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
5521 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
5581 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
5641 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
5701 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
5761 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
5821 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
5881 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
5941 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
6001 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
6061 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
6121 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
6181 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
6241 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
6301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
6361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
6421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
6481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
6541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
6601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
6661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
6721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
6781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
6841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
6901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
6961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
7021 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
7081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
7141 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
7201 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
7261 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
7321 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
7381 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
7441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
7501 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
7561 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
7621 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
7681 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
7741 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
7801 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
7861 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
7921 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
7981 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
8041 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
8101 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
8161 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
8221 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
8281 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
8341 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
8401 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
8461 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
8521 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
8581 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
8641 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
8701 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
8761 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
8821 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
8881 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
8941 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
9001 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
9061 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
9121 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
9181 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
9241 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
9301 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
9361 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
9421 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
9481 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
9541 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
9601 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
9661 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
9721 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
9781 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
9841 caaaattttc gggtttatta cagggacagc agagatccag tttggactag tccacaccct
9901 aactgacaca ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
9961 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
10021 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
10081 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
10141 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcag
//
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