LOCUS hprtSAGFP 11351 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION In vivo single-strand annealing substrate composed of two truncated
GFP genes, separated by a drug selection marker, and flanked by
mouse hprt targeting sequences.
ACCESSION .
VERSION .
KEYWORDS hprtSAGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11351)
AUTHORS Stark JM, Pierce AJ, Oh J, Pastink A, Jasin M
TITLE Genetic steps of mammalian homologous repair with distinct mutagenic
consequences.
JOURNAL Mol Cell Biol. 2004 Nov;24(21):9305-16.
PUBMED 15485900
REFERENCE 2 (bases 1 to 11351)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11351
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 21..125
/gene="bla"
/label=AmpR promoter
CDS 126..986
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(344..363)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1157..1745
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1646..1665
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1899..1916
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2033..2054
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2069..2099
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2107..2123
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2112..2134
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2131..2147
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2131..2147
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 2168..2186
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 2223..2239
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2223..2239
/label=pBluescriptSK
/note="For pBluescript vector"
enhancer 3566..3945
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 4223..5239
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 5163..5185
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 5247..5266
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 5294..5303
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 5300..5818
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN173
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIE"
primer_bind complement(5345..5366)
/label=EGFP-N
/note="EGFP, reverse primer"
promoter 6056..6553
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(6091..6114)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 6486..6505
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 6572..7171
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6572..6591)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7068..7088
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind complement(7629..7648)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 7694..7749
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(7748..7767)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind 8682..8703
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(8907..8926)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(10707..10726)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(10708..10726)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(10733..10750)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(10733..10749)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10742..10764)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 10891..11346
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10978..10997)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 11188..11209
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
2221 gctctagaac tagtggatcc gcggccgcta attcgattct tctgtaaaat tatcttatta
2281 gtaagttgta accatctcag acttctaagt ttgagtttgt aaaggatcag ttttttttgg
2341 tgacctacac acacacacac acacacacac acacacacac acacaggggg gtggattctg
2401 agaatttaga gaatttagat ggttaatacc agtttttatt tttattctgt gtgatattta
2461 tttattaaga tttatttatt tattacatgt aggtacactg tatctgtctt cagacacacc
2521 agaagagggc atcagatctc gttacagatg gttgtgagtc accatgtggt tgctgggatt
2581 tgaactcagg acctctggaa gaacagtcag tgctcttaac cgttgagcca tctcaccagc
2641 cccgtgatat tttatttaac ttactagaat atactgtgtt aaaacatatt ttaggggatt
2701 ggggagatgg cccattctgt aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa
2761 ccccagcatg gagaagacag agacagggag acccctgggc ttcctggcaa gctagactag
2821 caaatttggt taactaggtt caatgagaga ctcagtctca aaacataaga agtgaatttt
2881 ttagacccaa tttcaatctc tgaactccat acgtatgcac atgtacctgc atataaatat
2941 atatacacac ccataaacac atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta
3001 taagagtaga aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc agcacttggg
3061 agcagaggca ggtggatttc tgagttcgag gccagcctgg tctacagagt gagttccagg
3121 acagccagag ctatacagag aaatcctgcc tcaaaaaacc aaaaataaag tgtatatact
3181 aatctttcat gcctaaaata tttatttttg ccacattttt catacctctt taatattttt
3241 ctttttctct ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc attcttgact
3301 ctattcagca gtaagacgca gcatggcttt cttagagaag tataatctat tacaaaacgg
3361 taacatttaa ctgctctaca taccctcttg tagctaaatt cctctgttaa actaagactt
3421 attatctaaa cgtaattaaa tgttgatatt attgaagcat ttatcagggt tattgtctca
3481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
3541 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca
3601 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
3661 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
3721 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
3781 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
3841 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
3901 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
3961 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
4021 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggsgcg cgccrggsgg
4081 ggsggggsgg ggsgrggggs ggggsggggs grggsggrga ggkgcggcgg cagccaatca
4141 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
4201 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
4261 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
4321 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
4381 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
4441 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
4501 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
4561 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
4621 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
4681 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
4741 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
4801 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
4861 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
4921 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
4981 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
5041 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
5101 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
5161 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
5221 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
5281 tggcaaagaa ttcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
5341 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
5401 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
5461 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
5521 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
5581 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
5641 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
5701 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
5761 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
5821 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
5881 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
5941 gaagtgagaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
6001 caatgccctg gctcacaaat accactgaga tctgatatca tcgatgaatt ctaccgggta
6061 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
6121 tggcgctaca caagtggcct ctggcctcac acattccaca tccaccggta ggcgccaacc
6181 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
6241 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
6301 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
6361 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
6421 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
6481 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
6541 tccgggcctt tcgaccgatc cagccgccac catgaccgag tacaagccca cggtgcgcct
6601 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
6661 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
6721 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
6781 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
6841 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
6901 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
6961 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
7021 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
7081 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
7141 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
7201 cgaaaggagc gcacgacccc atggctccga ccgaagccac ccggggcggc cccgccgacc
7261 ccgcacccgc ccccgaggcc caccgcgggg gacacaccga acacgccgac cctgctgaac
7321 acgcggcgca gttcggtgcc caggagcgga tcgaaattga tgatctatta aacaataaag
7381 atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg agaacagagt
7441 acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg ggattagata
7501 aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt tcatagttgg
7561 atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc ccactcatga
7621 tctatagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
7681 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
7741 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
7801 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
7861 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
7921 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
7981 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
8041 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagctttaa
8101 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
8161 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
8221 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
8281 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
8341 ggcaactaca agacccgcgc cgaggtgaag ttcgataggg ataacagggt aatccgcatc
8401 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
8461 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
8521 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
8581 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
8641 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
8701 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
8761 tctagatcat aatcagccat accacatttg tggaggtttt acttgcttaa aaaacctccc
8821 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
8881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
8941 tttttcactg aagcttgttt ttatttgtat ctttaaattt tatgatagat gttatagtgt
9001 actctcctct cccctcccct cccctctcct cctcagcagg atcttgttat gtagccctaa
9061 gggatctaat acttactctg tagtccaggc tgtccttcag ctcatgagac acaccatcat
9121 gcttggcatg tttcttgttc tgaaaggtat ctggttttaa ttttggttga gctggtgtgt
9181 tggtgtatat ctgtaatctc agcacaggca agtagagaca aggattatga ttacagtcca
9241 gcctgggcta tttagaggga ttctctttaa tctttcctca tgccccaaaa tcttaccttt
9301 ggtatatgaa aaatagtctc cacttctgca aaatattgct ttatgaagta agaattccct
9361 tcatagagac aaggaatgtg tcctgtaaaa gtttaatgtg taagaagtat ttgttataaa
9421 agataaatat tcagaatctt ctttttaatt cctgatttta tttctatagg actgaaagac
9481 ttgctcgaga tgtcatgaag gagatgggag gccatcacat tgtggccctc tgtgtgctca
9541 aggggggcta taagttcttt gctgacctgc tggattacat taaagcactg aatagaaata
9601 gtgatagatc cattcctatg actgtagatt ttatcagact gaagagctac tgtgtaagta
9661 taattaactt ataattaaaa aaatagggcc attctagttt tatctatatt ttttttaaac
9721 ttgtgcaaac tatgctacat aatcaactta cttgattatg taatatatat agtaatagtt
9781 ggaattacta ttattacata tttatttttc tgtgctgggg tagaacccag ggaactaagt
9841 actgtaataa ccgagcttca ttcatcctag tcatttttaa tttttttttt tttttttgtt
9901 ttttcgagac aaggtttctc tgtgtagccc tggctgtcct ggaactcact ctgtagacca
9961 ggctggcctc gaactcagaa atctgcctgc ctctgcctcc cagtgctgag attaatgacg
10021 tgcaccacca ctgcctggca tttttttaaa gttttaattt gagacccttt ctcactaact
10081 tgctcacact atcctcgaac tcattacgtg ggctggtcta gaaatttcag tcaacttgcc
10141 tcagcttctc aagtagctgg gattacaggc aggtgctacc acttctggcc atgatcattg
10201 ttgttgttgc tattattatt attattattt cactgatact tcatatcaca acacagaata
10261 gtaactgtag ttcatgacca acaaaaaagt tgtacagttt ctctgttgtc ttattttgat
10321 ctggattttg atttgtttca tggcactggg caggctaatc tcaaactgca tggctcaaat
10381 agtccttctg tcttaatctc ccatgttctg gaagtatagg tatcactaca cctagctgta
10441 ttttcatatt ttaaaaaaaa cgcctaagaa ttgctattga atgaggcgga gagatggctc
10501 agcagtcaca agtacttgta gaggactggg gtttggttac tagcacctca aagatggctt
10561 acaattatct gtagctccaa ttccagagga tctgtgtggt aaagctgcat gtatatgggc
10621 atgcaggtaa aactgctaaa catataaaaa tagctcagtc ttaagagaat tggtcgacct
10681 cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacaat tcactggccg
10741 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
10801 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
10861 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
10921 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
10981 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
11041 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
11101 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
11161 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
11221 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
11281 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
11341 caatttaggt g
//
