| 质粒名称: | pLYS1-FLAG-MitoGFP-HA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 50057 |
| 存储实验室: | Vamsi Mootha |
|
载体骨架基本信息 |
|
| 载体名称: | pLYS1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7331 bp |
| 完整质粒大小: | 8341 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Mito-GFP |
| 物种来源: | -- |
| 插入大小: | 717 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: |
Flag（插入N端子）
HA（插入件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Nhe1(未破坏) |
| 3' 克隆位点: | EcoR1 (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLYS1-FLAG-MitoGFP-HA was a gift from Vamsi Mootha (Addgene plasmid # 50057 ; http://n2t.net/addgene:50057 ; RRID:Addgene_50057) |
| 参考文献部分: | EMRE is an Essential Component of the Mitochondrial Calcium Uniporter Complex. Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O, Mootha VK. Science. 2013 Nov 14. 10.1126/science.1242993 PubMed 24231807 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLYS1-FLAG-MitoGFP-HA 8341 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression of GFP targeted to mitochondria with Flag and HA tags.
ACCESSION .
VERSION .
KEYWORDS pLYS1-FLAG-MitoGFP-HA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8341)
AUTHORS Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi
ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O,
Mootha VK
TITLE EMRE is an Essential Component of the Mitochondrial Calcium
Uniporter Complex.
JOURNAL Science. 2013 Nov 14.
PUBMED 24231807
REFERENCE 2 (bases 1 to 8341)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8341
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..227
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 228..408
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 455..580
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1073..1306
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1491..1535
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
primer_bind 1833..1853
/label=hU6-F
/note="Human U6 promoter, forward primer"
promoter 2142..2345
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2296..2316
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 2560..2583
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2614..3330
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2614..3330
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2659..2680)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2920..2939)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3267..3288
/label=EGFP-C
/note="EGFP, forward primer"
CDS 3358..3384
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
misc_feature 3435..3552
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3601..4111
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 4030..4048
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 4133..4732
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4133..4152)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4629..4649
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 4860..5093
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5165..5286
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5202..5221)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5256..5275
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5326..5461
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5388..5407
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5481..5500)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5482..5500)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5510..5527)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5510..5526)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5519..5541)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5668..6123
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5755..5774)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5965..5986
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6149..6253
/gene="bla"
/label=AmpR promoter
CDS 6254..7114
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6472..6491)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7285..7873
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7774..7793
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8027..8044
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8161..8182
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8197..8227
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8235..8251
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8240..8262
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8259..8275
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8259..8275
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8294..8314
/label=T3
/note="T3 promoter, forward primer"
promoter 8296..8314
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
1801 gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
1861 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
1921 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
1981 ttttaaaatt atgttttaaa atggactatc atatgccaag tacgccccct attgacgtca
2041 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
2101 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2161 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2221 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2281 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2341 gagctggttt agtgaaccgt cagatccgct agcatggcgg ccgccgcagg tagatcgctc
2401 ctgctgctcc tctcctctcg gggcggcggc ggcgggggcg ccggcggctg cggggcgctg
2461 actgccggct gcttccctgg gctgggcgtc agccgccacc ggcagcagca gcaccaccgg
2521 acggtacacc agaggatcgc ttccggaggc accggtatgg attacaagga tgacgatgac
2581 aagggtggat ctggtggatc tggtggaccc gggatggtga gcaagggcga ggagctgttc
2641 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
2701 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
2761 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
2821 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
2881 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
2941 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
3001 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
3061 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
3121 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
3181 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
3241 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
3301 atcactctcg gcatggacga gctgtacaag ggtggatctg gtggatctgg tggatcttac
3361 ccctatgatg tgcctgacta cgcataaggt accgaattct cgacctcgag acaaatggca
3421 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
3481 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
3541 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag
3601 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
3661 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
3721 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
3781 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
3841 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
3901 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
3961 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
4021 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
4081 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
4141 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
4201 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
4261 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
4321 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
4381 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
4441 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
4501 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
4561 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
4621 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
4681 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
4741 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag
4801 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
4861 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
4921 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
4981 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
5041 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
5101 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
5161 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
5221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
5281 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
5341 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
5401 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
5461 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
5521 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
5581 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
5641 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
5701 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
5761 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
5821 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
5881 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
5941 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
6001 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
6061 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
6121 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
6181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
6241 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
6301 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
6361 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
6421 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
6481 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
6541 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
6601 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
6661 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
6721 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
6781 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
6841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
6901 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
6961 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
7021 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
7081 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
7141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
7201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
7261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
7321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
7381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
7441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
7501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
7561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
7621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
7681 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
7741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
7801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
7861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
7921 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
7981 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
8041 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
8101 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
8161 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
8221 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
8281 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
8341 a
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pLV-ER GFP
- 下一篇：pHAGE2-TetOminiCMV-SKM
