质粒名称: | CDA1-BE3 |
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出品公司: | Addgene |
目录编号: | 100804 |
存储实验室: | David Liu |
载体骨架基本信息 |
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载体名称: | pCMV |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 3402 bp |
完整质粒大小: | 8469 bp |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | CDA1-BE3 |
物种来源: | 合成;化脓链球菌 |
插入大小: | 5067 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | CMV |
标签/融合蛋白: | -- |
克隆方法: | 吉布森克隆 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | T7 |
3' 测序引物 | M13R |
文献引用方法 |
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材料和方法部分: | CDA1-BE3 was a gift from David Liu (Addgene plasmid # 100804 ; http://n2t.net/addgene:100804 ; RRID:Addgene_100804) |
参考文献部分: | Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. aao4774 [pii] PubMed 28875174 |
买家导航
CDA1BE3载体基本信息
CDA1BE3载体图谱质粒图谱和多克隆位点信息
CDA1BE3载体简介
CDA1BE3载体序列
LOCUS CDA1-BE3 8469 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Expresses CDA1-BE3 in mammalian cells. ACCESSION . VERSION . KEYWORDS CDA1-BE3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8469) AUTHORS Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR TITLE Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. JOURNAL Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. PUBMED 28875174 REFERENCE 2 (bases 1 to 8469) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8469 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 132..335 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 286..306 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 377..396 /label=T7 /note="T7 promoter, forward primer" promoter 377..395 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 409..1032 /codon_start=1 /product="cytidine deaminase from the sea lamprey Petromyzon marinus (Rogozin et al., 2007)" /label=PmCDA1 /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV" CDS 1081..5181 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(D10A) /note="generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5194..5442 /codon_start=1 /product="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 5455..5475 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5484..5501 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(5524..5541) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5530..5754 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(5825..5841) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5825..5841) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5838..5860) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5849..5865 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5873..5903) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5918..5939 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6056..6073) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6227..6815) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6307..6326) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6986..7846) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7609..7628 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7847..7951) /gene="bla" /label=AmpR promoter primer_bind complement(8030..8049) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 8221..131 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gacagacgct 421 gaatatgtta ggatccatga aaaactggat atctatacat ttaagaagca gttcttcaat 481 aacaaaaagt cagtatctca cagatgctat gtcctgttcg aactcaagag aagaggagaa 541 aggcgggcct gtttctgggg gtacgcggtt aataaacccc agtccgggac cgagaggggg 601 attcacgccg agatcttttc aattaggaag gttgaagagt atcttcgcga caatcccggt 661 cagttcacaa ttaactggta cagctcctgg agcccttgcg ctgattgcgc cgagaaaata 721 ctcgaatggt acaaccagga gttgagaggc aatggccaca ctctcaagat ttgggcttgc 781 aagctttact acgagaagaa cgcgagaaat cagattggct tgtggaacct cagggacaac 841 ggggtcgggt tgaatgttat ggtgtccgaa cattaccagt gctgtagaaa gatcttcatt 901 cagtccagtc acaatcagct gaacgagaac agatggctgg agaaaacact gaaacgggca 961 gagaaaaggc gctcagagct gagtatcatg atccaggtca aaatcctgca tacaaccaaa 1021 agcccggctg taagcggcag cgagactccc gggacctcag agtccgccac acccgaaagt 1081 gataaaaagt attctattgg tttagccatc ggcactaatt ccgttggatg ggctgtcata 1141 accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac agaccgtcat 1201 tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac ggcagaggcg 1261 actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg aatatgttac 1321 ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt tcaccgtttg 1381 gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat ctttggaaac 1441 atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct cagaaaaaag 1501 ctagttgact caactgataa agcggacctg aggttaatct acttggctct tgcccatatg 1561 ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa ctcggatgtc 1621 gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga gaaccctata 1681 aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa atcccgacgg 1741 ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt cggtaacctt 1801 atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt agctgaagat 1861 gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct actggcacaa 1921 attggagatc agtatgcgga cttatttttg gctgccaaaa accttagcga tgcaatcctc 1981 ctatctgaca tactgagagt taatactgag attaccaagg cgccgttatc cgcttcaatg 2041 atcaaaaggt acgatgaaca tcaccaagac ttgacacttc tcaaggccct agtccgtcag 2101 caactgcctg agaaatataa ggaaatattc tttgatcagt cgaaaaacgg gtacgcaggt 2161 tatattgacg gcggagcgag tcaagaggaa ttctacaagt ttatcaaacc catattagag 2221 aagatggatg ggacggaaga gttgcttgta aaactcaatc gcgaagatct actgcgaaag 2281 cagcggactt tcgacaacgg tagcattcca catcaaatcc acttaggcga attgcatgct 2341 atacttagaa ggcaggagga tttttatccg ttcctcaaag acaatcgtga aaagattgag 2401 aaaatcctaa cctttcgcat accttactat gtgggacccc tggcccgagg gaactctcgg 2461 ttcgcatgga tgacaagaaa gtccgaagaa acgattactc catggaattt tgaggaagtt 2521 gtcgataaag gtgcgtcagc tcaatcgttc atcgagagga tgaccaactt tgacaagaat 2581 ttaccgaacg aaaaagtatt gcctaagcac agtttacttt acgagtattt cacagtgtac 2641 aatgaactca cgaaagttaa gtatgtcact gagggcatgc gtaaacccgc ctttctaagc 2701 ggagaacaga agaaagcaat agtagatctg ttattcaaga ccaaccgcaa agtgacagtt 2761 aagcaattga aagaggacta ctttaagaaa attgaatgct tcgattctgt cgagatctcc 2821 ggggtagaag atcgatttaa tgcgtcactt ggtacgtatc atgacctcct aaagataatt 2881 aaagataagg acttcctgga taacgaagag aatgaagata tcttagaaga tatagtgttg 2941 actcttaccc tctttgaaga tcgggaaatg attgaggaaa gactaaaaac atacgctcac 3001 ctgttcgacg ataaggttat gaaacagtta aagaggcgtc gctatacggg ctggggacga 3061 ttgtcgcgga aacttatcaa cgggataaga gacaagcaaa gtggtaaaac tattctcgat 3121 tttctaaaga gcgacggctt cgccaatagg aactttatgc agctgatcca tgatgactct 3181 ttaaccttca aagaggatat acaaaaggca caggtttccg gacaagggga ctcattgcac 3241 gaacatattg cgaatcttgc tggttcgcca gccatcaaaa agggcatact ccagacagtc 3301 aaagtagtgg atgagctagt taaggtcatg ggacgtcaca aaccggaaaa cattgtaatc 3361 gagatggcac gcgaaaatca aacgactcag aaggggcaaa aaaacagtcg agagcggatg 3421 aagagaatag aagagggtat taaagaactg ggcagccaga tcttaaagga gcatcctgtg 3481 gaaaataccc aattgcagaa cgagaaactt tacctctatt acctacaaaa tggaagggac 3541 atgtatgttg atcaggaact ggacataaac cgtttatctg attacgacgt cgatcacatt 3601 gtaccccaat cctttttgaa ggacgattca atcgacaata aagtgcttac acgctcggat 3661 aagaaccgag ggaaaagtga caatgttcca agcgaggaag tcgtaaagaa aatgaagaac 3721 tattggcggc agctcctaaa tgcgaaactg ataacgcaaa gaaagttcga taacttaact 3781 aaagctgaga ggggtggctt gtctgaactt gacaaggccg gatttattaa acgtcagctc 3841 gtggaaaccc gccaaatcac aaagcatgtt gcacagatac tagattcccg aatgaatacg 3901 aaatacgacg agaacgataa gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa 3961 ttggtgtcgg acttcagaaa ggattttcaa ttctataaag ttagggagat aaataactac 4021 caccatgcgc acgacgctta tcttaatgcc gtcgtaggga ccgcactcat taagaaatac 4081 ccgaagctag aaagtgagtt tgtgtatggt gattacaaag tttatgacgt ccgtaagatg 4141 atcgcgaaaa gcgaacagga gataggcaag gctacagcca aatacttctt ttattctaac 4201 attatgaatt tctttaagac ggaaatcact ctggcaaacg gagagatacg caaacgacct 4261 ttaattgaaa ccaatgggga gacaggtgaa atcgtatggg ataagggccg ggacttcgcg 4321 acggtgagaa aagttttgtc catgccccaa gtcaacatag taaagaaaac tgaggtgcag 4381 accggagggt tttcaaagga atcgattctt ccaaaaagga atagtgataa gctcatcgct 4441 cgtaaaaagg actgggaccc gaaaaagtac ggtggcttcg atagccctac agttgcctat 4501 tctgtcctag tagtggcaaa agttgagaag ggaaaatcca agaaactgaa gtcagtcaaa 4561 gaattattgg ggataacgat tatggagcgc tcgtcttttg aaaagaaccc catcgacttc 4621 cttgaggcga aaggttacaa ggaagtaaaa aaggatctca taattaaact accaaagtat 4681 agtctgtttg agttagaaaa tggccgaaaa cggatgttgg ctagcgccgg agagcttcaa 4741 aaggggaacg aactcgcact accgtctaaa tacgtgaatt tcctgtattt agcgtcccat 4801 tacgagaagt tgaaaggttc acctgaagat aacgaacaga agcaactttt tgttgagcag 4861 cacaaacatt atctcgacga aatcatagag caaatttcgg aattcagtaa gagagtcatc 4921 ctagctgatg ccaatctgga caaagtatta agcgcataca acaagcacag ggataaaccc 4981 atacgtgagc aggcggaaaa tattatccat ttgtttactc ttaccaacct cggcgctcca 5041 gccgcattca agtattttga cacaacgata gatcgcaaac gatacacttc taccaaggag 5101 gtgctagacg cgacactgat tcaccaatcc atcacgggat tatatgaaac tcggatagat 5161 ttgtcacagc ttgggggtga ctctggtggt tctactaatc tgtcagatat tattgaaaag 5221 gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa 5281 gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc 5341 accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg 5401 gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag 5461 aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 5521 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 5581 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 5641 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 5701 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 5761 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat 5821 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 5881 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa 5941 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6001 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 6061 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 6121 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 6181 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 6241 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 6301 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 6361 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 6421 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6481 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6541 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6601 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 6661 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 6721 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 6781 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 6841 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6901 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 7141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 7201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 7261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 7321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 7621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 7741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7921 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7981 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct 8041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 8101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 8161 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 8221 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 8281 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 8341 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 8401 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 8461 aagtgtatc //