PX390

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX390 或 PX390 Flavobacterium columnare Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 11801 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68327
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX390载体图谱



载体简介

载体序列

LOCUS       PX390 Flavobacterium columnare Cas9	11801 bp 	DNA	SYN
DEFINITION  PX390 Flavobacterium columnare Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="PX390 Flavobacterium columnare Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..6492
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMTKILGLDLGTNSIGWAIRDTEN
                     EGINQILDKGARIFSEGVKSENGKEISRAAERTAYRSARKIKYRRKLRKYETLKVLSI
                     NGMCPLSVEEVEQWKKSGFKEYPLNPEFLDWLRTNEDKNINPYLFRDKASKQKVTLFE
                     LGRALYHIAQRRGFLSNRLDQSAEGVFEEHNPQIQNLIEDLDSSNTILNELKEYYINL
                     GIIDETEKSGFKKDLDEGEKKLKSLYNSLVAITKKNANDIETCKQELIARLNKKEDLG
                     KVKGKIKDISQAMLDRNFKTLGQYFFSLYNKERIRNQYTSREEHYLEEFIIICQTQGI
                     EGINTNEKLPEKKFTGLAKDLYRAIFFQRPLKSQKGLIGKCSFEKNKSRCAVSHPDYE
                     EFRMWSYLNTIKIGTQSEKTLRFLTLEEKLKLVPKFYRKSDFNFEVLAKELVEKGASF
                     GYYKSSKKNEFFYWFNYKPTDSVSACVVSASLENAIGKDWKIKTFEYQTRNTEKNEVT
                     KSVDYKDLWHLLSVATSDTYLYDFAIEKLKLEPKNAKAFSKTKLKKDFASLSLAAIAK
                     ILPYLKQGLLYSHAVFMANIENIVDADIWKDEEQQKFIQSKIVELVDNYIVEKSKLEL
                     INGLLKIYNTEDKEGRKVYYSKEAESLFEADLRKKLVPFYKANIIIEEHEQEIIFQDL
                     FPILMDQLKKQEFIKIGRLDKQIEAFLEGENEEGQIFCNHTDKLKKLYHPSDIEVFKK
                     KTIKDEWGNEKVVLGSPLTTSIKNPMAMRALHQLRKVLNTLIANDQIDEDTRIHIEMA
                     RELNDANKRKGIQDFQNENKKFREEAIKEIKKLYLEECHKDVNPTEDDILRYQLWLEQ
                     GKCEIYEEGNNISICDIIGSNPSYDIEHTIPRSISQDNSQMNKTLCSLKFNRSIKKQK
                     MPVELSNYNDILPRIAHWKKEAEELTRQIETISRSIKSAATKVAKDKNIRKRHYLTLK
                     RDYILGKYERFTWEEPKVGFKNSQIPDTGIITKYAQAYLKSYFKRVESVKGGAVAEFR
                     KLWGIQESFIDENWWKHYKDKDRDKHTHHTIDAITIACMPKDKYDLLAHAWRLEDEQD
                     KKAAKVLIEQAKPWKTFKEDIEKIETEILVSHFTPDNVKKQSKSIIKNRGKKVYVLKN
                     ELPVNFKNKIEGKDYFKLKFDSKILYKIPKKKEKQTDTFYEELPKNYLNGVEGKDYFK
                     INTTGKTFYKIPIFNQGDTIRGSLHQETTYGAIKLPDIDIETKKPLHTDKGGFILKKD
                     IKGNEIVFFVVRKEISKISENDVQNIVDNVVRKKIENAIANSLITFKIVKKKKVAVIK
                     EGVTIWMREPNIEKGIEGIPIKKVRIITNSVKNPIEIKVHSPLSKSRHKHKQKVYGQN
                     DENYAMALYELDGKREFELINNFNLAKLLKQSQSYYPLHKEKEIKGKKILVPIEKRNN
                     KDVILKRGQQVVFYDKTVENPKDISEIIDFRERIYIIEGLTIQRQKDKKTSKVNEYGI
                     IQLRHFKEARKSEEISKDNFKPDGEFKINENKPTRKMNHNQFTAFVEGIDFKVLPSGK
                     FQKI*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMTKILGLDLGTNSIGWAIRDTEN
                     EGINQILDKGARIFSEGVKSENGKEISRAAERTAYRSARKIKYRRKLRKYETLKVLSI
                     NGMCPLSVEEVEQWKKSGFKEYPLNPEFLDWLRTNEDKNINPYLFRDKASKQKVTLFE
                     LGRALYHIAQRRGFLSNRLDQSAEGVFEEHNPQIQNLIEDLDSSNTILNELKEYYINL
                     GIIDETEKSGFKKDLDEGEKKLKSLYNSLVAITKKNANDIETCKQELIARLNKKEDLG
                     KVKGKIKDISQAMLDRNFKTLGQYFFSLYNKERIRNQYTSREEHYLEEFIIICQTQGI
                     EGINTNEKLPEKKFTGLAKDLYRAIFFQRPLKSQKGLIGKCSFEKNKSRCAVSHPDYE
                     EFRMWSYLNTIKIGTQSEKTLRFLTLEEKLKLVPKFYRKSDFNFEVLAKELVEKGASF
                     GYYKSSKKNEFFYWFNYKPTDSVSACVVSASLENAIGKDWKIKTFEYQTRNTEKNEVT
                     KSVDYKDLWHLLSVATSDTYLYDFAIEKLKLEPKNAKAFSKTKLKKDFASLSLAAIAK
                     ILPYLKQGLLYSHAVFMANIENIVDADIWKDEEQQKFIQSKIVELVDNYIVEKSKLEL
                     INGLLKIYNTEDKEGRKVYYSKEAESLFEADLRKKLVPFYKANIIIEEHEQEIIFQDL
                     FPILMDQLKKQEFIKIGRLDKQIEAFLEGENEEGQIFCNHTDKLKKLYHPSDIEVFKK
                     KTIKDEWGNEKVVLGSPLTTSIKNPMAMRALHQLRKVLNTLIANDQIDEDTRIHIEMA
                     RELNDANKRKGIQDFQNENKKFREEAIKEIKKLYLEECHKDVNPTEDDILRYQLWLEQ
                     GKCEIYEEGNNISICDIIGSNPSYDIEHTIPRSISQDNSQMNKTLCSLKFNRSIKKQK
                     MPVELSNYNDILPRIAHWKKEAEELTRQIETISRSIKSAATKVAKDKNIRKRHYLTLK
                     RDYILGKYERFTWEEPKVGFKNSQIPDTGIITKYAQAYLKSYFKRVESVKGGAVAEFR
                     KLWGIQESFIDENWWKHYKDKDRDKHTHHTIDAITIACMPKDKYDLLAHAWRLEDEQD
                     KKAAKVLIEQAKPWKTFKEDIEKIETEILVSHFTPDNVKKQSKSIIKNRGKKVYVLKN
                     ELPVNFKNKIEGKDYFKLKFDSKILYKIPKKKEKQTDTFYEELPKNYLNGVEGKDYFK
                     INTTGKTFYKIPIFNQGDTIRGSLHQETTYGAIKLPDIDIETKKPLHTDKGGFILKKD
                     IKGNEIVFFVVRKEISKISENDVQNIVDNVVRKKIENAIANSLITFKIVKKKKVAVIK
                     EGVTIWMREPNIEKGIEGIPIKKVRIITNSVKNPIEIKVHSPLSKSRHKHKQKVYGQN
                     DENYAMALYELDGKREFELINNFNLAKLLKQSQSYYPLHKEKEIKGKKILVPIEKRNN
                     KDVILKRGQQVVFYDKTVENPKDISEIIDFRERIYIIEGLTIQRQKDKKTSKVNEYGI
                     IQLRHFKEARKSEEISKDNFKPDGEFKINENKPTRKMNHNQFTAFVEGIDFKVLPSGK
                     FQKI*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMTKILGLDLGTNSIGWAIRDTEN
                     EGINQILDKGARIFSEGVKSENGKEISRAAERTAYRSARKIKYRRKLRKYETLKVLSI
                     NGMCPLSVEEVEQWKKSGFKEYPLNPEFLDWLRTNEDKNINPYLFRDKASKQKVTLFE
                     LGRALYHIAQRRGFLSNRLDQSAEGVFEEHNPQIQNLIEDLDSSNTILNELKEYYINL
                     GIIDETEKSGFKKDLDEGEKKLKSLYNSLVAITKKNANDIETCKQELIARLNKKEDLG
                     KVKGKIKDISQAMLDRNFKTLGQYFFSLYNKERIRNQYTSREEHYLEEFIIICQTQGI
                     EGINTNEKLPEKKFTGLAKDLYRAIFFQRPLKSQKGLIGKCSFEKNKSRCAVSHPDYE
                     EFRMWSYLNTIKIGTQSEKTLRFLTLEEKLKLVPKFYRKSDFNFEVLAKELVEKGASF
                     GYYKSSKKNEFFYWFNYKPTDSVSACVVSASLENAIGKDWKIKTFEYQTRNTEKNEVT
                     KSVDYKDLWHLLSVATSDTYLYDFAIEKLKLEPKNAKAFSKTKLKKDFASLSLAAIAK
                     ILPYLKQGLLYSHAVFMANIENIVDADIWKDEEQQKFIQSKIVELVDNYIVEKSKLEL
                     INGLLKIYNTEDKEGRKVYYSKEAESLFEADLRKKLVPFYKANIIIEEHEQEIIFQDL
                     FPILMDQLKKQEFIKIGRLDKQIEAFLEGENEEGQIFCNHTDKLKKLYHPSDIEVFKK
                     KTIKDEWGNEKVVLGSPLTTSIKNPMAMRALHQLRKVLNTLIANDQIDEDTRIHIEMA
                     RELNDANKRKGIQDFQNENKKFREEAIKEIKKLYLEECHKDVNPTEDDILRYQLWLEQ
                     GKCEIYEEGNNISICDIIGSNPSYDIEHTIPRSISQDNSQMNKTLCSLKFNRSIKKQK
                     MPVELSNYNDILPRIAHWKKEAEELTRQIETISRSIKSAATKVAKDKNIRKRHYLTLK
                     RDYILGKYERFTWEEPKVGFKNSQIPDTGIITKYAQAYLKSYFKRVESVKGGAVAEFR
                     KLWGIQESFIDENWWKHYKDKDRDKHTHHTIDAITIACMPKDKYDLLAHAWRLEDEQD
                     KKAAKVLIEQAKPWKTFKEDIEKIETEILVSHFTPDNVKKQSKSIIKNRGKKVYVLKN
                     ELPVNFKNKIEGKDYFKLKFDSKILYKIPKKKEKQTDTFYEELPKNYLNGVEGKDYFK
                     INTTGKTFYKIPIFNQGDTIRGSLHQETTYGAIKLPDIDIETKKPLHTDKGGFILKKD
                     IKGNEIVFFVVRKEISKISENDVQNIVDNVVRKKIENAIANSLITFKIVKKKKVAVIK
                     EGVTIWMREPNIEKGIEGIPIKKVRIITNSVKNPIEIKVHSPLSKSRHKHKQKVYGQN
                     DENYAMALYELDGKREFELINNFNLAKLLKQSQSYYPLHKEKEIKGKKILVPIEKRNN
                     KDVILKRGQQVVFYDKTVENPKDISEIIDFRERIYIIEGLTIQRQKDKKTSKVNEYGI
                     IQLRHFKEARKSEEISKDNFKPDGEFKINENKPTRKMNHNQFTAFVEGIDFKVLPSGK
                     FQKI*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMTKILGLDLGTNSIGWAIRDTEN
                     EGINQILDKGARIFSEGVKSENGKEISRAAERTAYRSARKIKYRRKLRKYETLKVLSI
                     NGMCPLSVEEVEQWKKSGFKEYPLNPEFLDWLRTNEDKNINPYLFRDKASKQKVTLFE
                     LGRALYHIAQRRGFLSNRLDQSAEGVFEEHNPQIQNLIEDLDSSNTILNELKEYYINL
                     GIIDETEKSGFKKDLDEGEKKLKSLYNSLVAITKKNANDIETCKQELIARLNKKEDLG
                     KVKGKIKDISQAMLDRNFKTLGQYFFSLYNKERIRNQYTSREEHYLEEFIIICQTQGI
                     EGINTNEKLPEKKFTGLAKDLYRAIFFQRPLKSQKGLIGKCSFEKNKSRCAVSHPDYE
                     EFRMWSYLNTIKIGTQSEKTLRFLTLEEKLKLVPKFYRKSDFNFEVLAKELVEKGASF
                     GYYKSSKKNEFFYWFNYKPTDSVSACVVSASLENAIGKDWKIKTFEYQTRNTEKNEVT
                     KSVDYKDLWHLLSVATSDTYLYDFAIEKLKLEPKNAKAFSKTKLKKDFASLSLAAIAK
                     ILPYLKQGLLYSHAVFMANIENIVDADIWKDEEQQKFIQSKIVELVDNYIVEKSKLEL
                     INGLLKIYNTEDKEGRKVYYSKEAESLFEADLRKKLVPFYKANIIIEEHEQEIIFQDL
                     FPILMDQLKKQEFIKIGRLDKQIEAFLEGENEEGQIFCNHTDKLKKLYHPSDIEVFKK
                     KTIKDEWGNEKVVLGSPLTTSIKNPMAMRALHQLRKVLNTLIANDQIDEDTRIHIEMA
                     RELNDANKRKGIQDFQNENKKFREEAIKEIKKLYLEECHKDVNPTEDDILRYQLWLEQ
                     GKCEIYEEGNNISICDIIGSNPSYDIEHTIPRSISQDNSQMNKTLCSLKFNRSIKKQK
                     MPVELSNYNDILPRIAHWKKEAEELTRQIETISRSIKSAATKVAKDKNIRKRHYLTLK
                     RDYILGKYERFTWEEPKVGFKNSQIPDTGIITKYAQAYLKSYFKRVESVKGGAVAEFR
                     KLWGIQESFIDENWWKHYKDKDRDKHTHHTIDAITIACMPKDKYDLLAHAWRLEDEQD
                     KKAAKVLIEQAKPWKTFKEDIEKIETEILVSHFTPDNVKKQSKSIIKNRGKKVYVLKN
                     ELPVNFKNKIEGKDYFKLKFDSKILYKIPKKKEKQTDTFYEELPKNYLNGVEGKDYFK
                     INTTGKTFYKIPIFNQGDTIRGSLHQETTYGAIKLPDIDIETKKPLHTDKGGFILKKD
                     IKGNEIVFFVVRKEISKISENDVQNIVDNVVRKKIENAIANSLITFKIVKKKKVAVIK
                     EGVTIWMREPNIEKGIEGIPIKKVRIITNSVKNPIEIKVHSPLSKSRHKHKQKVYGQN
                     DENYAMALYELDGKREFELINNFNLAKLLKQSQSYYPLHKEKEIKGKKILVPIEKRNN
                     KDVILKRGQQVVFYDKTVENPKDISEIIDFRERIYIIEGLTIQRQKDKKTSKVNEYGI
                     IQLRHFKEARKSEEISKDNFKPDGEFKINENKPTRKMNHNQFTAFVEGIDFKVLPSGK
                     FQKI*"
     CDS             complement(3754..5181)
                     /label="ORF frame 3"
                     /translation="MAHQDFSLNLFDILLEGFPWFGLLDQHLSGLFVLFILQPPGVCQ
                     QVVFILGHTGNSDCVDCMVGMFVSIFVLIVLPPVLIDKRFLDAPQFPELSHCSSLDTL
                     HPFEVRFQICLSVLGNDPSVWDLGVFKAHFRFLPSEPLVFPQNIVSLQCQVMPLSDVL
                     ILGHLGCSALDAARNGFDLPSQFLGLLFPVCDPGQNVIVVGQLHWHLLLFDGSVELQA
                     TQCLVHLAVILGDAPRNGMLDVIGGIAANDITNADIVAFLVDLALSLFQPQLIPQDIV
                     LGGVHVFVAFLQVQLLDLLDGFLPKLLVLVLEVLDAFSLISIVQFSGHLNMDPSILVD
                     LVVGNQCIQHLPQLVQGSHCHGVLDRCGQWGSQDHLFIPPLIFDSLFLKHLDVAWMVQ
                     FLQLICVVTKDLSFLIFPLQEGFNLLIQSANFDELLFLQLVHQDRKQILENDFLLVFL
                     DDDVSLVEGDQFLSQVCLEQAFSLL*"
     CDS             complement(3810..4295)
                     /label="ORF frame 1"
                     /translation="MASSRNFLFSFWKSWMPFRLLASFSSLAISIWIRVSSSIWSLAI
                     SVFSTFLSWCRALIAMGFLIDVVSGDPRTTFSFPHSSLIVFFLNTSMSLGWYSFFSLS
                     VWLQKICPSSFSPSRKASICLSSLPILMNSCFFSWSIRIGNRSWKMISCSCSSMMMLA
                     L*"
     CDS             complement(5209..5931)
                     /label="ORF frame 3"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     misc_feature    complement(6525..6542)
                     /label="Sp6_primer"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     misc_feature    6547..6571
                     /label="attB2"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     terminator      6583..7049
                     /label="hGH_PA_terminator"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     terminator      7055..7090
                     /label="T7_terminator"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     rep_origin      7207..7513
                     /label="f1_origin"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     misc_feature    complement(7627..7647)
                     /label="pBABE_3_primer"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     misc_feature    complement(7633..7848)
                     /label="SV40_enhancer"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     promoter        7645..7913
                     /label="SV40_promoter"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     rep_origin      7812..7889
                     /label="SV40_origin"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     misc_feature    7874..7893
                     /label="SV40pro_F_primer"
                     /translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
                     PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
                     NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
                     LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
                     EVHRQLVLQDVHLFAAILDDAL*"
     CDS             8028..8822
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            8031..8819
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(8337..8873)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8999..9118
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    9087..9106
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      9309..9344
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(9367..9384)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    9374..9451
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(9481..10098)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    10078..10111
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(10879..11739)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(10879..11739)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatgacc aaaatcctgg ggctggacct ggggactaat
 1921 agcatcgggt gggcaatccg cgacacagaa aatgagggca tcaatcagat cctggacaag
 1981 ggcgcccgca ttttcagcga gggagtgaag tccgagaatg gcaaagaaat cagtagggca
 2041 gctgaacgaa ccgcttaccg gagcgcaaga aagatcaaat atcggagaaa actgcggaag
 2101 tacgagacac tgaaggtgct gtcaattaac ggaatgtgcc ccctgagcgt ggaggaagtc
 2161 gaacagtgga agaaatctgg cttcaaggaa tatcccctga atcctgagtt tctggattgg
 2221 ctgcggacca acgaggacaa gaacatcaat ccttacctgt tcagagataa agctagcaag
 2281 cagaaagtga cactgtttga actgggaaga gctctgtatc acatcgcaca gaggcgcggc
 2341 ttcctgagta acaggctgga ccagtcagcc gagggcgtct ttgaggaaca taatcctcag
 2401 atccagaacc tgattgagga cctggacagc tccaacacaa ttctgaatga gctgaaggaa
 2461 tactatatca atctggggat cattgacgag actgaaaagt ccggcttcaa gaaagacctg
 2521 gatgaggggg aaaagaaact gaagtcactg tacaacagcc tggtggccat cacaaagaaa
 2581 aacgctaacg atatcgaaac ttgcaagcag gagctgatcg ctagactgaa taagaaagag
 2641 gacctgggca aggtcaaagg gaagatcaaa gatatttctc aggcaatgct ggacaggaac
 2701 tttaagactc tgggacagta tttcttttca ctgtacaaca aggagcgaat ccggaaccag
 2761 tataccagcc gcgaggaaca ctacctggag gagttcatca ttatctgtca gacccagggg
 2821 atcgagggaa ttaacacaaa tgagaagctg cctgagaaga agttcaccgg cctggcaaaa
 2881 gatctgtatc gagccatttt ctttcagcgg ccactgaaat cccagaaggg cctgatcggg
 2941 aagtgctctt tcgagaagaa caagtctcgc tgtgccgtga gtcacccaga ctatgaggaa
 3001 tttcgaatgt ggagctacct gaacacaatc aaaattggca ctcagtccga gaaaaccctg
 3061 cgcttcctga cactggagga aaagctgaaa ctggtgccca agttctaccg aaagagtgat
 3121 ttcaacttcg aggtgctggc taaggagctg gtcgaaaaag gagcatcatt cggctactac
 3181 aagtctagta agaaaaatga gttcttttac tggttcaact ataagcccac cgattcagtg
 3241 agcgcctgcg tggtgagcgc ttctctggag aacgcaatcg gcaaggactg gaagatcaag
 3301 actttcgaat atcagactag aaacaccgag aagaatgaag tgaccaaatc cgtcgattac
 3361 aaggacctgt ggcacctgct gtccgtggca acatctgata cttacctgta tgactttgcc
 3421 atcgaaaagc tgaaactgga gcctaaaaac gcaaaggcct tcagcaagac aaaactgaag
 3481 aaagactttg ccagtctgtc actggcagcc atcgctaaaa ttctgccata tctgaagcag
 3541 ggcctgctgt actcccacgc cgtgttcatg gctaacatcg agaatattgt cgacgccgat
 3601 atctggaagg atgaggaaca gcagaagttc atccagtcca agattgtgga gctggtcgac
 3661 aattatatcg tggaaaagtc taaactggag ctgatcaatg ggctgctgaa gatctacaac
 3721 accgaggata aggaaggacg gaaagtgtac tattcaaagg aggctgaaag cctgttcgag
 3781 gcagacctga gaaagaaact ggtccccttc tacaaggcta acatcatcat cgaggaacac
 3841 gagcaggaaa tcattttcca ggatctgttt cctatcctga tggaccagct gaagaaacag
 3901 gagttcatca aaattggcag actggataag cagattgaag ccttcctgga gggggaaaat
 3961 gaggaaggac agatcttttg taaccacaca gataagctga agaaactgta ccatccaagc
 4021 gacatcgagg tgtttaagaa aaagactatc aaagatgagt gggggaatga aaaggtggtc
 4081 ctgggatccc cactgaccac atctatcaag aaccccatgg caatgagagc cctgcaccag
 4141 ctgaggaagg tgctgaatac actgattgcc aacgaccaga tcgacgagga tactcggatc
 4201 catattgaga tggccagaga actgaacgat gctaataagc gaaaaggcat ccaggacttc
 4261 cagaacgaga acaagaagtt tcgggaggaa gccatcaagg agatcaagaa gctgtacctg
 4321 gaggaatgcc acaaagacgt gaaccccacc gaggacgata tcctgaggta tcagctgtgg
 4381 ctggaacagg gaaagtgcga gatctacgag gaaggcaaca atatcagcat ttgtgatatc
 4441 attggcagca atccctccta tgacatcgag cataccattc ctcggagcat ctcccaggat
 4501 aacagccaga tgaacaagac actgtgtagc ctgaagttca acagatccat caaaaagcag
 4561 aagatgccag tggagctgtc caactacaat gacattctgc ccaggatcgc acactggaaa
 4621 aaggaggccg aggaactgac taggcagatc gaaaccattt ctcgcagcat caagagcgct
 4681 gcaaccaagg tggccaagga taagaacatc agaaagaggc attacctgac actgaagcga
 4741 gactatattc tggggaaata cgagcggttc acttgggagg aacctaaagt gggctttaaa
 4801 aactcccaga tcccagacac tgggatcatt accaagtacg ctcaggcata tctgaaatct
 4861 tacttcaaaa gggtggagag tgtcaaggga ggagcagtgg ctgagttccg gaaactgtgg
 4921 ggcatccagg aatcttttat cgatgagaac tggtggaagc actataagga caaagataga
 4981 gacaaacata cccaccatac aatcgacgca atcactattg cctgtatgcc caaggataaa
 5041 tacgacctgc tggcacacgc ctggaggctg gaggatgaac aggacaaaaa ggccgctaag
 5101 gtgctgatcg agcaggccaa accatggaaa accttcaagg aggatatcga aaagattgag
 5161 actgaaatcc tggtgagcca ttttaccccc gacaacgtca aaaagcagtc aaagagcatc
 5221 atcaagaatc gcggcaaaaa ggtgtacgtc ctgaagaacg agctgcctgt gaacttcaag
 5281 aacaagatcg aagggaagga ttatttcaag ctgaaatttg acagcaagat tctgtacaaa
 5341 atccccaaaa agaaagagaa gcagaccgat acattctatg aggaactgcc taaaaactac
 5401 ctgaatggag tggaaggcaa ggactacttc aaaatcaata ctaccggcaa gaccttctac
 5461 aaaatcccaa tttttaacca gggcgacaca atccggggga gcctgcacca ggagacaact
 5521 tacggagcca ttaagctgcc agatatcgac attgaaacaa agaaacccct gcatactgat
 5581 aagggaggct tcattctgaa gaaagacatc aaaggcaatg agattgtgtt ctttgtggtc
 5641 cgcaaggaaa tctctaaaat tagtgagaac gatgtgcaga atatcgtcga caacgtggtc
 5701 cggaagaaaa ttgagaatgc tatcgcaaac tccctgatca cttttaagat cgtgaagaaa
 5761 aagaaagtgg ccgtcatcaa agagggggtc accatttgga tgagggagcc caacattgaa
 5821 aaggggatcg agggaatccc tattaagaaa gtgcgcatca ttaccaattc tgtcaaaaac
 5881 cctatcgaga ttaaggtgca cagtccactg tccaagtctc gccacaagca taaacagaag
 5941 gtctacggcc agaacgatga gaattatgcc atggctctgt acgaactgga cgggaagcgg
 6001 gagttcgaac tgatcaacaa ttttaatctg gccaagctgc tgaaacagag tcagtcatac
 6061 tatccactgc ataaagagaa ggaaatcaag ggaaagaaaa ttctggtgcc catcgagaag
 6121 agaaacaaca aggatgtcat cctgaagagg ggccagcagg tggtgttcta cgacaaaacc
 6181 gtggagaacc ccaaggatat ctctgaaatc attgactttc gcgagcgaat ctacatcatt
 6241 gaagggctga ccatccagag acagaaagat aagaaaacat ccaaagtgaa tgagtacgga
 6301 atcattcagc tgcgccactt caaggaagct cgaaaaagtg aggaaatctc aaaggataac
 6361 ttcaaacctg acggcgagtt caagatcaac gagaataagc caactaggaa aatgaaccat
 6421 aatcagttca ccgcctttgt ggaggggatc gacttcaagg tcctgcctag cggaaagttt
 6481 cagaaaattt aagaattcgg taccgagctc aagcttgcgg ccgcctatag tgtcacctaa
 6541 atcgtaaccc agctttcttg tacaaagtgg tcgggtggca tccctgtgac ccctccccag
 6601 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
 6661 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
 6721 tggtatggag caaggggcaa gttgggatga caacctgtag ggcctgcggg gtctattggg
 6781 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
 6841 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
 6901 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
 6961 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
 7021 cgtgaaccac tgctcccttc cctgtccttc tagcataacc ccttggggcc tctaaacggg
 7081 tcttgagggg ttttttgtct agaagacaat agcaggcatg ctggggatgc ggtgggctct
 7141 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
 7201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
 7261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
 7321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
 7381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
 7441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
 7501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
 7561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
 7621 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
 7681 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
 7741 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
 7801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
 7861 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
 7921 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
 7981 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
 8041 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
 8101 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
 8161 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
 8221 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
 8281 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
 8341 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
 8401 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
 8461 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
 8521 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
 8581 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
 8641 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
 8701 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
 8761 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
 8821 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
 8881 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
 8941 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
 9001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
 9061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
 9121 tgtctgttca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
 9181 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
 9241 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
 9301 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagca
 9361 tgcggaggaa attctccttg aagtttccct ggtgttcaaa gtaaaggagt ttgcaccaga
 9421 cgcacctctg ttcactggtc cggcgtatta aaacacgata cattgttatt agtacattta
 9481 ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
 9541 ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
 9601 gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
 9661 agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
 9721 caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
 9781 tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
 9841 ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata gagcttggac
 9901 atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
 9961 gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
10021 gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaattg cgatcgcata
10081 acttcgtata gcatacatta tacgaagtta taagctcgga acgctgcgct cggtcgttcg
10141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
10201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
10261 ggccgcgttg ctggcgtttt tccataggct ccgccccctg acgagcatca caaaaatcga
10321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
10381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
10441 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
10501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
10561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
10621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
10681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
10741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
10801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
10861 tctcaagaag atcctttgtt accaatgctt aatcagtgag gcacctatct cagcgatctg
10921 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
10981 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
11041 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
11101 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
11161 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
11221 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
11281 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
11341 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
11401 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
11461 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
11521 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
11581 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
11641 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
11701 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
11761 ttgaagcatt tatcagggtt attgtctcat gtccgcgcgt t
//

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