PX389

价格：1000元

联系方式：I47-825O-882O

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PX389载体质粒基本信息

出品公司:	Addgene
载体名称:	PX389 或 PX389 Fluviicola taffensis Cas9
质粒类型:	CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体
高拷贝/低拷贝:	高拷贝
插入位点:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	11570 bp
5' 测序引物及序列:	CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:	C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签:	NLS (C ter)；HA、NLS (N ter)
载体抗性:	氨苄青霉素
筛选标记:	Neomycin
克隆菌株:	Stbl3
宿主细胞（系）:	昆虫细胞
备注:	--
产品目录号:	#68326
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	杆状病毒

PX389质粒图谱载体图谱和PX389载体序列质粒序列多克隆位点信息

PX389载体图谱

PX389质粒载体简介

PX389质粒序列载体序列

LOCUS       PX389 Fluviicola taffensis Cas9	11570 bp 	DNA	SYN
DEFINITION  PX389 Fluviicola taffensis Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="PX389 Fluviicola taffensis Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             complement(1795..2502)
                     /label="ORF frame 3"
                     /translation="MKLHQLIWCLFLFLASQLIVSPFLIEVQQDPCQFLLLNFLRQSF
                     LSQIVDCPVMGNFLAVAHQLWLVDFKVDQHFLVALLKQEHVISIETDFWLRFRLELAE
                     PLLKVNLVGIVFWQEANDIQHSMQPLAPQQSSVDQSPDSPQSSSLSCLTDRVAFSELV
                     LNILCHRNDPAAHAQDLPLFVIEVLVNQCPSNAVSPQIQSQNSLCHGRFDLALFLRRS
                     VIWNIVWVHGGTGGISP*"
     CDS             1822..6261
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
                     FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
                     HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
                     HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
                     TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
                     IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
                     LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
                     HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
                     SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
                     LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
                     EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
                     IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
                     LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
                     DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
                     DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
                     QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
                     QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
                     LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
                     LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
                     TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
                     KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
                     KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
                     LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
                     VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
                     GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
                     ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
                     FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
                     HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
                     HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
                     TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
                     IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
                     LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
                     HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
                     SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
                     LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
                     EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
                     IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
                     LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
                     DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
                     DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
                     QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
                     QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
                     LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
                     LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
                     TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
                     KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
                     KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
                     LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
                     VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
                     GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
                     ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
                     FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
                     HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
                     HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
                     TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
                     IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
                     LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
                     HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
                     SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
                     LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
                     EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
                     IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
                     LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
                     DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
                     DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
                     QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
                     QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
                     LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
                     LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
                     TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
                     KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
                     KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
                     LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
                     VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
                     GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
                     ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
                     FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
                     HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
                     HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
                     TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
                     IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
                     LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
                     HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
                     SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
                     LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
                     EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
                     IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
                     LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
                     DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
                     DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
                     QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
                     QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
                     LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
                     LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
                     TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
                     KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
                     KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
                     LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
                     VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
                     GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
                     ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
     CDS             complement(4525..5097)
                     /label="ORF frame 3"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     misc_feature    complement(6294..6311)
                     /label="Sp6_primer"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     misc_feature    6316..6340
                     /label="attB2"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     terminator      6352..6818
                     /label="hGH_PA_terminator"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     terminator      6824..6859
                     /label="T7_terminator"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     rep_origin      6976..7282
                     /label="f1_origin"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     misc_feature    complement(7396..7416)
                     /label="pBABE_3_primer"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     misc_feature    complement(7402..7617)
                     /label="SV40_enhancer"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     promoter        7414..7682
                     /label="SV40_promoter"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     rep_origin      7581..7658
                     /label="SV40_origin"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     misc_feature    7643..7662
                     /label="SV40pro_F_primer"
                     /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                     SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                     DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                     HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
     CDS             7797..8591
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            7800..8588
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(8106..8642)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8768..8887
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8856..8875
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      9078..9113
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(9136..9153)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    9143..9220
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(9250..9867)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    9847..9880
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(10648..11508)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(10648..11508)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatataccA CCGGTGCCAC CATGTACCCA TACGATGTTC CAGATTACGC TTCGCCGAAG
 1861 AAAAAGCGCA AGGTCGAAGC GTCCATGACA AAGACTATTC TGGGACTGGA TCTGGGGACT
 1921 AACAGCATTG GATGGGCACT GATTAACCAG GACTTCGATA ACAAAAAGGG GGAGATCCTG
 1981 GGCATGGGCA GCCGGATCAT TCCTATGACA CAGGATATTA AGGACGAGTT CGGAAAAGGC
 2041 AACTCTATCA GTCAGACAGC TGAGCGAACT CGACTGCGGG GAGTCCGGAG ACTGATCCAC
 2101 AGAACTCTGC TGAGGCGCGA GCGGCTGCAT AGAGTGCTGA ATATCATTGG CTTCCTGCCA
 2161 GAACACTATG CCAACCAGAT TGACTTTGAG AAGAGGTTCG GCAAGTTCAA GCCTGAATCT
 2221 GAGCCAAAAA TCAGTTTCGA TGGAAATGAC GTGTTCCTGT TTGAGAAGAG CTACCAGGAA
 2281 ATGCTGGTCG ACTTTAAAAT CCACCAGCCA CAGCTGGTGA GCAACGGCAA GAAAATTCCC
 2341 CATGACTGGA CAATCTACTA TCTGAGAAAG AAAGCTCTGT CTAAGAAAAT TGAGAAGGAG
 2401 GAACTGGCAT GGATCCTGCT GAACTTCAAT CAGAAACGGG GATACTATCA GCTGAGAGGC
 2461 GAGGAAGAGG AAGAGACACC AAATAAGCTG GTGGAGTTTC ATAGCCTGAA AATTGTGGAG
 2521 GTCAACGCCG ATGAACCCCA GAAAGGAAAG CCTGAGATCT GGTACTCACT GGTGCTGGAA
 2581 AACGGCTGGG TCTATCGACG GGCTAGCAAG ACTCCCCTGT TCGATTGGAA AGACAAGATT
 2641 AGGGAGTTTA TCGTGACCAC AGAAATCAAC AATGATGGAA CTGTCAAAAC CGACAAGGAG
 2701 GGCACCGAAA AGAGGAGCTT CCGCGCACCA AAACCCGAGG ACTGGACTCT GCAGAAGAAA
 2761 AAGACCGAGT TCGAGCTGTC AAAGAGCGGG AATGAAGTGG GAGCCTTCAT CTACGAAAGT
 2821 ATCCTGCAGA AACCCAACCA GAAGATTAGA GGCAAACTGA TTTCAACCAT CGAGAGGAAA
 2881 TTTTATAAGG AAGAGCTGAA AACCATCCTG AAAACACAGC TGTTCTTTCA CAAAGAACTG
 2941 AAGGATGAGA AACTGTACAA TGCCTGCATC GAAGAGCTGT ATAAGAACAA TGAAGCTCAC
 3001 CGGAGCCTGC TGTCCAACAA GGGGTTCGAG CATCTGTTTA TTAACGACAT CCTGTTCTAC
 3061 CAGCGACCTC TGCGGTCTAA AAAGAGTCAG ATCTCAAACT GCCCACTGGA GAAGCGCACA
 3121 TATAAAAAGG AGGGGATTGA AATCACTGAG GGCATCAAAG TGATCTCCAA ATCTAATCCA
 3181 ATCTACCAGG AGTTCCGGCT GTGGCAGTGG ATTAGCAACC TGTCCCTGTA TTGTATCGAA
 3241 CCCACCGAGA CAAATGTGAC TTCAACCTTT CTGAACAGCA TTGAAGATTA CGAGAATCTG
 3301 TTCGAATTTC TGAACAATCG CAAGGAAATC GAGCAGAAGC ACCTGCTGAA ATATCTGCTG
 3361 GAGAACCAGG GGTTTAAAGG AAAGCTGCTG ACAAACGAAC TGGAGAAGTT CCGCTGGAAT
 3421 TTTGTCGCTG ACAAAAAGTA CCCCTGTAAT GAGACAGGCA GCCTGCTGCA TACTCGGCTG
 3481 AGCAAAGTGA AGAACATTTC CCCTGATTTC CTGACCAAGG AAATCGAGCA CCAGCTGTGG
 3541 CATATCATCT ACAGCGTGAC CGACAAGATT GAATATGAGC AGGCCCTGAA AACCTTTGCT
 3601 CGGAAAAACA ATCTGGATGT GGACTCCTTC TTTGAGCACT TCAAAAAGAT CCCCCCTTTT
 3661 GAGTCTACCT ACGGAGCATA TAGTGAAAAG GCCATCAAGA AGCTGCTGCC ACTGATCAGA
 3721 CTGGGCAAAT ACTGGAACTG GGAGGCCATT GATAGTATCT CAAAGGACAG GATTAGTAAA
 3781 ATCCTGTCAG GGGAATACGA TGAGAACATT AAGAACAGAG TGAGGGAGAA AGCAGTCCAC
 3841 CTGACCTCCG AAAACAATTT CCAGGGACTG CAGGAGTGGC TGGCCAAGTA CATCGTCTAT
 3901 GATCGCCATT CTGAGGGCAA TGACCTGGGG AAGTGGACTA GCGTGTCCGA CCTGGAGACA
 3961 TACCTGAAGG AGTTCAAGCA GCATAGCCTG CGGAACCCTA TTGTGGAGCA GGTCATCACA
 4021 GAAACTCTGA GAGTGGTCAA GGATATTTGG ATCAAGCACG GGAAAGGAAC CGAAAATTTC
 4081 TTTGACGAAA TCCATGTGGA GCTGGGCCGG GAAATGAAGA ACAATTCCGA GGATCGCAAA
 4141 CGACTGACCA ACACAATTAC TGAAAACGAG AATACAAACC TGAGAATCAA GGCCCTGCTG
 4201 ATGGAAATGA TGAATGATAA CGACGTGGAG AACGTCAGGC CTTACTCTCC AAGTCAGCAG
 4261 GAGATTCTGA AGATCTATGA GGACGGAGCT CTGAATAGCA ACATCGAGCT GGACGATGAA
 4321 ATTGTGAAGA TCTCCAAAAA GGCAGAGCCC ACCAAATCTG AACTGCAGCG CTACAAGCTG
 4381 TGGCTGGAGC AGAAATACCG ATCCCCTTAT ACTGGCCAGG TCATCCCACT GAACAAGCTG
 4441 TTCACCTCTG AATATGAGAT CGAACACGTG GTCCCTCAGA GTCGCTTCTT TGACGATAGC
 4501 TTCAGCAACA AAGTGATCTG CGAGTCAGCC GTCAACAAGC GGAAGGATAA CCAGCTGGGG
 4561 CTGCAGTTCA TCAAGAACCA TAGCGGAGAA AAAGTGGAGC TGGGCTTCGG GAAGGTGGTC
 4621 CAGGTCTTTA CAGAAGAGCA GTACCTGGAT TTTGTGAAGG AGCACTATAG CAAAAATCGC
 4681 TCCAAGCATA ACAAACTGCT GCTGGAAGAG ATTCCCGAGA AGATGATCGA AAGGCAGCTG
 4741 AATGACACTC GCTACATCAG TAAGTTCGTG AGCTCCATTC TGTCCAACAT CGTCAGATCT
 4801 GAGAAGGACG ATGACGGCCT GAATAGCAAA AACATTCTGC CTGGAAATGG CAAGATCACT
 4861 ACCGAACTGA AAAGGGACTG GGGGCTGAAT GATGTGTGGA ACGACCTGAT TCTGCCAAGA
 4921 TTCGAGAGGA TGAATACCAT CACAAACAGC GATCTGTTTA CAACTTACAA CGACAAGTAT
 4981 CAGAAACACC TGCCCACCGT GCCTTTCGAG TACTCCAAGG GCTTTCAGAA AAAGCGCATT
 5041 GATCACCGAC ACCATGCTAT GGACGCACTG GTCATCGCAT GTGCCACACG GGATCATCTG
 5101 AATCTGATGA ACAATCAGTC TGCCAAGAGT GAACTGAAAC GATACGACCT GCAGAACAAG
 5161 CTGCGGAAAA AGGAGCCTTA CTTCAACCAG AAGGAGAACA AACAGAAGGA AGCCTTCAAG
 5221 GATTTTATCA AACCATGGGG CACTTTCACC GAGGACAGCA AGAATGCTCT GGAAAAAATC
 5281 ATTATCTCCT TTAAGCAGAA CCTGAGAGTG ATCAACAAGG CAACAAACTC ATACGAGAGC
 5341 TATAAGGATG AGGACGGGAA TCTGAGGATC GGCAAGGATG GGAAACCAGA GAAGGGCCTG
 5401 ATCAAGCAGA AGGGGCTGAA CTGGGCAATC AGAAAGCCCC TGCACAAAGA CACCGTGTCA
 5461 GGCCAGATTA ACCTGAGCAG GATCAAGCTG CCCAATGGGA AAATCCTGAC AGCCACTCGC
 5521 AAGAATCTGG ATACCAGTTT CGACCTGAAA ACAATTGAGA ACTCAATCAC CGACACAGGC
 5581 ATTCAGAAAA TCCTGAAGAA TTACCTGCTG TCCAAGGAAT CTCCAGAGCT GGCCTTCTCT
 5641 CCCGAGGGCC TGGAAGAGAT GAACAATGAG ATCGAAAAGT ACAACAATGG GAAATTTCAC
 5701 CATCCTATTA ACAAGGCTAG GATCTATGAA CTGGGGAGCA AATTCAATGT CGGACAGACA
 5761 GGCAACAGAA GGGATAAGTT TGTGGAGGCC GCTAAAGGAA CTAATCTGTT CTTTGCTATC
 5821 TACCAGGACG AGAATAAGAA CCGCTCTTAT GAAACTATTC CCCTGAACGA AGTGATCGAG
 5881 CACCAGAAGT GGCGAGCAAG TCTGCCTAAG GAAGAGCAGG AGAAAATTCC ACTGGTGCCC
 5941 GTCAACAAGC TGAAGGGGAC CTTCATCTTT TCCCTGTCTC CCAACGATCT GGTGTACGTC
 6001 CCTTCCAATG ACGAGCTGGA ACGAAGTGCT TCAATTGACT TCTCTAAGCT GAAAAAGGAA
 6061 CAGATCAACC GGCTGTATAA AATGGTGTCT AGTTCAGGAT CCCAGTGCTT CTTTGTGAAG
 6121 AGTGAGGTCG CAACCTCAGT GGTCAACAAA ATGGAATACA GCAGCCTGAA CAAGATGGAG
 6181 AAGTCTATCG ATAACCTGAT GGTGAAGGAG ATTTGTATCA AACTGAAGAT TGACAGGCTG
 6241 GGCAACATCA GCAAGGCCTA AGAATTCggt accgagctca agcttgcggc cgcctatagt
 6301 gtcacctaaa tcgtaaccca gctttcttgt acaaagtggt cgggtggcat ccctgtgacc
 6361 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
 6421 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
 6481 ggaggggggt ggtatggagc aaggggcaag ttgggatgac aacctgtagg gcctgcgggg
 6541 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
 6601 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
 6661 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
 6721 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
 6781 gattacaggc gtgaaccact gctcccttcc ctgtccttct agcataaccc cttggggcct
 6841 ctaaacgggt cttgaggggt tttttgtcta gaagacaata gcaggcatgc tggggatgcg
 6901 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
 6961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
 7021 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
 7081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
 7141 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
 7201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
 7261 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
 7321 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
 7381 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
 7441 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
 7501 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
 7561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
 7621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
 7681 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
 7741 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
 7801 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
 7861 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
 7921 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
 7981 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
 8041 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
 8101 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
 8161 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
 8221 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
 8281 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
 8341 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
 8401 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
 8461 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
 8521 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
 8581 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
 8641 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
 8701 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
 8761 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
 8821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
 8881 atcttatcat gtctgttcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
 8941 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
 9001 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
 9061 gcccatatgt aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
 9121 ctgaaagcat gcggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt
 9181 tgcaccagac gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta
 9241 gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta
 9301 ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg
 9361 attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag
 9421 gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg
 9481 attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat
 9541 attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt
 9601 tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag
 9661 agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc
 9721 ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct
 9781 attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattgc
 9841 gatcgcataa cttcgtatag catacattat acgaagttat aagctcggaa cgctgcgctc
 9901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
 9961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
10021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgccccctga cgagcatcac
10081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
10141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
10201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
10261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
10321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
10381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
10441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
10501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
10561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
10621 aaaaaaggat ctcaagaaga tcctttgtta ccaatgctta atcagtgagg cacctatctc
10681 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
10741 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
10801 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
10861 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
10921 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
10981 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
11041 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
11101 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
11161 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
11221 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
11281 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
11341 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
11401 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
11461 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
11521 tcaatattat tgaagcattt atcagggtta ttgtctcatg tccgcgcgtt
//

pUG6	pBD-NF-kB
ISceI-GR-RFP	pSET152
pSUMO	pIJ702-del35-55
pAcGFP1-N2	pRSET-EGFP
pEF1/V5-HisA	Pgex-HRV3C
pCRE-DD-AmCyan1	pPIC ZαGB
pLVX-DD-ZsGreen1 Control	pET-32a(+)
pSH65	pCGN978
pNEO	pOrf681_TA
pLexm	pCas9D10A-GFP
pET-12c(+)	pAN7-1
pMTD1.3xA	pENTR11-Dual
pET-41a(+)	pAD-SV40T
pVPack-GP	pLLP-OmpA
pdKeima-Red-S1	pHSG422
PX543	pDsRed-Monomer
pDC515	pHS85
pTet-Splice	pCRE-MetLuc2-Reporter
pTi	pUC4-KISS
pKLAC1	pLNCX2
pACT-MyoD	pWF1
pBad43	pcDNA3.1-GP130v1
pET-21a(+)	pJSC73
pRB373	pMDLg/pRRE
pProLabel-C	pREP4
pcDNA6.2-DsRed2-MCS1 miR	pOM2
pBT-4	pSC101-EcoRImeth+
pUCTag	pGL4.38[luc2P/p53 RE/Hygro]
c-Flag pcDNA3	pMT-Bip-V5-HisA
EWD299	pCREj-2
pHV23	pMal-c2G
pIκB-EGFP	pFLAG-CMV-3
pET-32 EK/LIC	pGL4.41[luc2P/HSE/Hygro]
pCL-Ampho	pGL4.72[hRlucCP]
pNEO-ars	pAGO
pCYM1	pBluescript II KS(-)
pBBR1MCS-2	pGL4.45[luc2P/ISRE/Hygro]
pPIC9k-His	pIC20H
pHY15	pcDNA5/FRT/V5-His-TOPO
pET-28a-BFP-C	pCDH-CMV-MCS-EF1a-RFP+BSD
pcDNA4/HisMax B	pEF6/myc-His B
pCH2	pATPase_TA
pPIC9	pL-brevisGH3-MBP
pSC101	pALEX a,b,c
pSUPER.neo+gfp	pHB-1
pMal-c4X	pcDNA3.1(+)/CAT
pTP-4	pFLAG-CMV2
pDEST26	pFA1
pBAD18	pML21
pcDNA5/FRT	pSP72

pLJC5-3XHA-EGFP-PEX26	pT3-Neo-EF1a-GFP
Tet-O-FUW-Neurod1	LeGO-G
pRAD1	pCAS9-mCherry empty
pQUAST>stop>mCD8-GFP	pMal-T-Avi-His/BirA
EC2_10_dCas9_VPR_HC_sgRNA	pAAVS1-PC-GCaMP6f
pAC1404-pCR8-mRuby2_NLSPUFa	pHAGE-PTCH1
pCXLE-hOCT3/4	EC2_3_dCas9_Mxi1_sgRNA
pRC-CMV-Rev1b	Lenti-luciferase-P2A-Neo
pSR47	pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK
1306 pSG5L HA FKHR wt	AbVec1.1-IGKC
pAAV2/8	TAL3393
pcDNA3.4_7C11-1 heavy chain	pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV
pCMV-hA3G-BE3	phage UbiC NLS HA stdMCP stdHalo
pGL3 2 kb prom. CD274	3XMEF2-luc
p58sheLL	pMXs-hOCT4 (Plath)
pUCC001	pGGA008
MSCV-(pBabe mcs)- human p210BCR-ABL-IRES	pTRE Tight ArchT-GFP
pAAVS1-PDi-CRISPRn	pAc-Neo-OVA
pXAT2	pAraGFPCDF
pSBtet-GP	pCMV-MMLVgag-3xNES-ABE8e
pYES-mtGFP	PB_tre_Cas9
401 pSG5L	pLenti X2 Puro DEST (w16-1)
pICH86966::AtU6p::sgRNA_PDS	pLentiV2-dCas9-VP64
pTEF:ATP	FUmGW
BECLIN-HA WT	pLenti-DsRed_IRES_MAPT:EGFP
pPPEM	pDIRECT_23D
pAAV-EFS-CjCas9-eGFP-HIF1a	pM-Cas9
pLenti-EML4-ALK variant 3a	pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
NM9-SpCas9-NLS3	pEGFP-LC3
pMonAID_nCas9-PmCDA_Hyg_ALS	pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I
GST-HA-GFP11-N1	pNeae2
pAc-sgRNA-Cas9	pEGFP-puro
CKS2 gRNA (BRDN0001149143)	p201G Cas9
pLVX-M-puro	LB1366 (Bcl-2 promoter with MHS1234)
pSELECT-HA-mFOXO1	pMJ329: pCMV-MmeFz2
ilux pGEX(-)	pCW-Cas9
VVT1	pAAV-EF1a-FLEX-GTB
pC003 - LshC2C2 locus into pACYC184 for	pGH335_MS2-AID*Δ-Hygro
pET28a-At-TRL	pCS2TAL3-DD
pCDH-puro-Bcl-XL	pBHt2
pNK9	pT4-CMV-GFP
pM1s3AsR_TS-C7	pCS2TAL3-RR
800 pSG5L HA PTEN wt	pNEU-hMbPylRS-4xU6M15
pJKR-L-tetR	PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a
BPK3082	Cbh_v5 AAV-CBE N-terminal
c-myc-PT3EF1a	pNN9
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-	pTRKH2
pMSCV-IRES-GFP II (pMIG II)	pFC334
pMSCV-hygro-STING	pCAG-GBP1-10gly-lexADBD
EC1000	pJSC131 - Bacterial expression plasmid f

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