| 出品公司: | Addgene |
|---|---|
| 载体名称: | PX391 或 PX391 Sphaerochaeta globus str. Buddy Cas9 |
| 质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 10733 bp |
| 5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
| 载体标签: | NLS (C ter)；HA、NLS (N ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 昆虫细胞 |
| 备注: | -- |
| 产品目录号: | #68328 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PX391 Sphaerochaeta globus str. Buddy Cas9 10733 bp DNA SYN
DEFINITION PX391 Sphaerochaeta globus str. Buddy Cas9
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..10733
/organism="PX391 Sphaerochaeta globus str. Buddy Cas9"
/mol_type="other DNA"
misc_feature 47..1001
/label="Ac_5_flank"
CDS complement(343..948)
/label="ORF frame 3"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1023..1587
/label="CMV_immearly_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1090..1377
/label="CAG_enhancer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1544..1564
/label="CMV_fwd_primer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1615..1633
/label="T7_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1633..1656
/label="lacO"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1772..1796
/label="attB1"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
CDS 1822..5424
/label="ORF frame 1"
/translation="MYPYDVPDYASPKKKRKVEASMSKKVSRRYEEQAQEICQRLGSR
PYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFIPSTGAAERRQKRGQRNSLR
HRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAVVRANPYELRLKGLNEQLTL
SELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKEKGISTFIEVLT
AFNTNGLIGYRNSESVKSKGVPVPTRDIISNEIDVLLQTQKQFYQEILSDEYCDRIVS
AILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAAKRY
QSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFR
RLEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEVVDALKTVSLIG
DYGPIGKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGS
TQALMGKYWHSAFKEKRDSEGFFKPNTNSDEEKYGRIANPVVHQTLNELRKLMNELIT
ILGAKPQEITVELARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIER
FRLLEDQAFVCPYCLEHISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIK
GKRTPYAAFSNTSAWGPIMHYLDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESD
NSYIAKAAKEYLRCLFNPNNVTAVGSLKGMETSILRKAWNLQGIDDLLGSRHWSKDAD
TSPTMRKNRDDNRHHGLDAIVALYCSRSLVQMINTMSEQGKRAVEIEAMIPIPGYASE
PNLSFEAQRELFRKKILEFMDLHAFVSMKTDNDANGALLKDTVYSILGADTQGEDLVF
VVKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPMEMKDKIEQLQSKNEAALQKYK
ESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVGGYYYLISNNKRTKTFVV
KEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEIIRKIQAMNPSYKPAYMKQGYSLYV
RLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVIIITFTEMGSGYQIYFSN
LAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCGE*"
misc_feature 1825..1851
/label="HA"
/translation="MYPYDVPDYASPKKKRKVEASMSKKVSRRYEEQAQEICQRLGSR
PYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFIPSTGAAERRQKRGQRNSLR
HRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAVVRANPYELRLKGLNEQLTL
SELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKEKGISTFIEVLT
AFNTNGLIGYRNSESVKSKGVPVPTRDIISNEIDVLLQTQKQFYQEILSDEYCDRIVS
AILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAAKRY
QSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFR
RLEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEVVDALKTVSLIG
DYGPIGKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGS
TQALMGKYWHSAFKEKRDSEGFFKPNTNSDEEKYGRIANPVVHQTLNELRKLMNELIT
ILGAKPQEITVELARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIER
FRLLEDQAFVCPYCLEHISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIK
GKRTPYAAFSNTSAWGPIMHYLDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESD
NSYIAKAAKEYLRCLFNPNNVTAVGSLKGMETSILRKAWNLQGIDDLLGSRHWSKDAD
TSPTMRKNRDDNRHHGLDAIVALYCSRSLVQMINTMSEQGKRAVEIEAMIPIPGYASE
PNLSFEAQRELFRKKILEFMDLHAFVSMKTDNDANGALLKDTVYSILGADTQGEDLVF
VVKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPMEMKDKIEQLQSKNEAALQKYK
ESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVGGYYYLISNNKRTKTFVV
KEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEIIRKIQAMNPSYKPAYMKQGYSLYV
RLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVIIITFTEMGSGYQIYFSN
LAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCGE*"
misc_feature 1855..1875
/label="SV40 NLS"
/translation="MYPYDVPDYASPKKKRKVEASMSKKVSRRYEEQAQEICQRLGSR
PYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFIPSTGAAERRQKRGQRNSLR
HRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAVVRANPYELRLKGLNEQLTL
SELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKEKGISTFIEVLT
AFNTNGLIGYRNSESVKSKGVPVPTRDIISNEIDVLLQTQKQFYQEILSDEYCDRIVS
AILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAAKRY
QSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFR
RLEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEVVDALKTVSLIG
DYGPIGKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGS
TQALMGKYWHSAFKEKRDSEGFFKPNTNSDEEKYGRIANPVVHQTLNELRKLMNELIT
ILGAKPQEITVELARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIER
FRLLEDQAFVCPYCLEHISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIK
GKRTPYAAFSNTSAWGPIMHYLDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESD
NSYIAKAAKEYLRCLFNPNNVTAVGSLKGMETSILRKAWNLQGIDDLLGSRHWSKDAD
TSPTMRKNRDDNRHHGLDAIVALYCSRSLVQMINTMSEQGKRAVEIEAMIPIPGYASE
PNLSFEAQRELFRKKILEFMDLHAFVSMKTDNDANGALLKDTVYSILGADTQGEDLVF
VVKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPMEMKDKIEQLQSKNEAALQKYK
ESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVGGYYYLISNNKRTKTFVV
KEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEIIRKIQAMNPSYKPAYMKQGYSLYV
RLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVIIITFTEMGSGYQIYFSN
LAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCGE*"
misc_feature 1861..1875
/label="NLS"
/translation="MYPYDVPDYASPKKKRKVEASMSKKVSRRYEEQAQEICQRLGSR
PYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFIPSTGAAERRQKRGQRNSLR
HRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAVVRANPYELRLKGLNEQLTL
SELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKEKGISTFIEVLT
AFNTNGLIGYRNSESVKSKGVPVPTRDIISNEIDVLLQTQKQFYQEILSDEYCDRIVS
AILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAAKRY
QSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFR
RLEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEVVDALKTVSLIG
DYGPIGKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGS
TQALMGKYWHSAFKEKRDSEGFFKPNTNSDEEKYGRIANPVVHQTLNELRKLMNELIT
ILGAKPQEITVELARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIER
FRLLEDQAFVCPYCLEHISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIK
GKRTPYAAFSNTSAWGPIMHYLDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESD
NSYIAKAAKEYLRCLFNPNNVTAVGSLKGMETSILRKAWNLQGIDDLLGSRHWSKDAD
TSPTMRKNRDDNRHHGLDAIVALYCSRSLVQMINTMSEQGKRAVEIEAMIPIPGYASE
PNLSFEAQRELFRKKILEFMDLHAFVSMKTDNDANGALLKDTVYSILGADTQGEDLVF
VVKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPMEMKDKIEQLQSKNEAALQKYK
ESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVGGYYYLISNNKRTKTFVV
KEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEIIRKIQAMNPSYKPAYMKQGYSLYV
RLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVIIITFTEMGSGYQIYFSN
LAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCGE*"
CDS complement(3289..3789)
/label="ORF frame 3"
/translation="MIHVCPSSCDICDANVLQTVRAHKGLILQQAEPFNIPFVQIVGL
AVLAICQHSLLFLFGLLLNDVLSFLSSDLQLSCQFHCDLLRFSSQDGNQLIHQLAQFV
QSLMDHWVGNPAVFFLIAICVRLKESLTVSLFFKSRVPILPHQGLSRSCQDLPIVGQQ
ALLLPH*"
CDS complement(4047..4589)
/label="ORF frame 1"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
misc_feature complement(5457..5474)
/label="Sp6_primer"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
misc_feature 5479..5503
/label="attB2"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
terminator 5515..5981
/label="hGH_PA_terminator"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
terminator 5987..6022
/label="T7_terminator"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
rep_origin 6139..6445
/label="f1_origin"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
misc_feature complement(6559..6579)
/label="pBABE_3_primer"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
misc_feature complement(6565..6780)
/label="SV40_enhancer"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
promoter 6577..6845
/label="SV40_promoter"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
rep_origin 6744..6821
/label="SV40_origin"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
misc_feature 6806..6825
/label="SV40pro_F_primer"
/translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
AVTLFGLNRQRRYSFAAFAI*"
CDS 6960..7754
/label="ORF frame 3"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 6963..7751
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(7269..7805)
/label="ORF frame 1"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 7931..8050
/label="SV40_PA_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 8019..8038
/label="EBV_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 8241..8276
/label="T7_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature complement(8299..8316)
/label="Baculovirs_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 8306..8383
/label="3_AcPH"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
CDS complement(8413..9030)
/label="ORF frame 3"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
misc_feature 9010..9043
/label="loxP"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
gene complement(9811..10671)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
CDS complement(9811..10671)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatgagc aagaaagtca gtagacgcta tgaagaacag
1921 gcacaggaga tttgtcagag gctggggagt agaccttatt ccatcgggct ggacctggga
1981 gtgggatcta tcggagtggc agtcgccgct tacgacccaa ttaagaaaca gccctccgat
2041 ctggtgttcg tcagctccag gatttttatc ccttctaccg gcgcagccga gcggagacag
2101 aagagaggac agaggaacag cctgcgccac cgagcaaatc gcctgaaatt cctgtggaag
2161 ctgctggctg aacgaaacct gatgctgtca tatagcgagc aggacgtgcc agatcctgca
2221 cggctgagat ttgaggacgc tgtggtccga gcaaacccat acgagctgcg gctgaagggc
2281 ctgaatgaac agctgaccct gagcgagctg gggtatgcac tgtaccacat cgccaatcat
2341 aggggatcta gttcagtgcg cacattcctg gatgaggaaa agagctccga cgataagaaa
2401 ctggaggaac agcaggctat gacagaacag ctggcaaaag agaagggaat ttccactttc
2461 atcgaagtgc tgacagcctt taacactaat ggcctgatcg ggtacaggaa ctccgagtct
2521 gtgaagagta agggcgtgcc agtccccact cgcgacatca tttcaaatga gattgatgtg
2581 ctgctgcaga cccagaagca gttctatcag gaaatcctgt cagacgagta ctgcgatcgg
2641 attgtcagcg caatcctgtt tgaaaacgag aagatcgtgc cagaagccgg ctgctgtccc
2701 tatttccctg acgagaagaa actgcccaga tgtcactttc tgaatgagga aaggcgcctg
2761 tgggaagcca ttaacaatgc taggatcaag atgcccatgc aggagggcgc tgcaaaacgc
2821 taccagagtg cttcattcag cgacgagcag agacacattc tgtttcatat cgcaaggagc
2881 gggactgata tcacccctaa actggtgcag aaggagttcc cagccctgaa aacctccatc
2941 attgtgctgc agggaaaaga gaaggctatt cagaagatcg caggcttccg atttcgacgg
3001 ctggaggaaa aatctttttg gaagagactg agtgaggaac agaaggacga tttctttagc
3061 gcctggacaa acactcctga cgataaaaga ctgtccaagt acctgatgaa acacctgctg
3121 ctgacagaaa atgaggtggt cgacgccctg aaaaccgtga gcctgattgg agattatggc
3181 ccaatcggca agaccgcaac acagctgctg atgaaacatc tggaggatgg cctgacttac
3241 accgaagccc tggagcgggg aatggaaacc ggcgagttcc aggaactgtc agtgtgggag
3301 cagcagagcc tgctgcccta ctatgggcag atcctgacag gatctactca ggccctgatg
3361 gggaagtatt ggcactctgc ttttaaagaa aagagagaca gtgagggatt ctttaagcct
3421 aacacaaata gcgatgagga aaaatacggc aggattgcca acccagtggt ccatcagact
3481 ctgaacgaac tgcgcaagct gatgaatgag ctgattacca tcctgggagc taaacctcag
3541 gagatcacag tggaactggc acgagagctg aaggtcggag ctgagaaaag agaggacatc
3601 attaagcagc agaccaaaca ggaaaaggag gctgtgctgg catatagcaa gtactgcgag
3661 cccaacaatc tggacaaaag gtatattgaa aggttccgcc tgctggagga tcaggccttt
3721 gtgtgccctt actgtctgga gcacattagc gtcgcagata tcgcagctgg aagggcagac
3781 gtggatcata tcttcccacg cgacgataca gctgacaact cctacgggaa taaggtggtc
3841 gcacaccgac agtgtaacga tatcaaggga aagcggaccc cctatgcagc cttcagtaat
3901 acatcagcct ggggccccat catgcattac ctggacgaaa cccctgggat gtggcgcaaa
3961 agaaggaagt ttgagacaaa cgaggaagag tatgctaagt acctgcagtc aaaaggcttc
4021 gtgagcaggt ttgaaagcga taactcctat atcgcaaaag ctgcaaagga gtacctgcgc
4081 tgcctgttca atccaaacaa tgtgactgcc gtcgggtccc tgaagggaat ggagacatct
4141 atcctgcgga aggcctggaa tctgcaggga attgacgatc tgctgggcag ccggcactgg
4201 agtaaggacg ccgataccag ccccacaatg cgcaaaaacc gggacgacaa tcggcaccat
4261 ggcctggacg ccatcgtggc tctgtattgt tccagatctc tggtccagat gattaacacc
4321 atgtccgagc agggcaagcg agcagtggaa atcgaggcta tgattcctat cccagggtac
4381 gcatccgaac caaatctgtc tttcgaagcc cagcgggagc tgtttagaaa gaaaatcctg
4441 gagttcatgg acctgcacgc ctttgtgagt atgaaaaccg acaacgatgc aaatggcgcc
4501 ctgctgaaag atactgtgta ttcaattctg ggagcagaca cccagggaga ggatctggtg
4561 ttcgtggtca agaaaaagat taaggacatc ggcgtgaaaa tcggggatta tgaagaggtc
4621 gcatctgcca ttcgaggccg gatcaccgac aaacagccaa agtggtatcc catggaaatg
4681 aaagataaga tcgagcagct gcagtctaag aacgaagccg ctctgcagaa atacaaggag
4741 agtctggtgc aggcagccgc tgtcctggaa gagagtaata ggaagctgat tgagtcaggc
4801 aaaaagccca tccagctgag tgaaaaaaca atttcaaaaa aggccctgga gctggtgggc
4861 gggtactatt acctgattag caacaacaag cgcacaaaga ctttcgtggt caaggaacct
4921 tcaaacgagg tgaaagggtt cgcatttgac actggaagca atctgtgcct ggacttttat
4981 cacgatgccc agggaaagct gtgtggcgag atcattagaa aaatccaggc tatgaaccct
5041 tcctataagc cagcatacat gaaacagggg tattctctgt acgtgagact gtaccagggc
5101 gacgtgtgcg agctgagggc aagcgatctg actgaagcag agtccaacct ggccaagacc
5161 acacatgtcc gcctgcccaa tgctaaacct gggcgaacct tcgtgatcat tatcaccttt
5221 acagagatgg ggtctggata tcagatctac ttcagcaacc tggccaagtc caaaaaggga
5281 caggacacta gttttaccct gactaccatc aagaattatg atgtgcggaa agtccagctg
5341 tctagtgccg ggctggtgag atacgtcagc cctctgctgg tggacaaaat cgagaaggat
5401 gaagtcgctc tgtgtggaga gtaagaattc ggtaccgagc tcaagcttgc ggccgcctat
5461 agtgtcacct aaatcgtaac ccagctttct tgtacaaagt ggtcgggtgg catccctgtg
5521 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
5581 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
5641 ggtggagggg ggtggtatgg agcaaggggc aagttgggat gacaacctgt agggcctgcg
5701 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
5761 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
5821 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
5881 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
5941 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctagcataa ccccttgggg
6001 cctctaaacg ggtcttgagg ggttttttgt ctagaagaca atagcaggca tgctggggat
6061 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
6121 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
6181 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
6241 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
6301 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
6361 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
6421 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
6481 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
6541 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
6601 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
6661 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
6721 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
6781 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
6841 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
6901 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
6961 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
7021 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
7081 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
7141 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
7201 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
7261 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
7321 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
7381 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
7441 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
7501 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
7561 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
7621 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
7681 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
7741 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
7801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
7861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
7921 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
7981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
8041 tgtatcttat catgtctgtt catgaagccc cttgagcatc tgacttctgg ctaataaagg
8101 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
8161 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
8221 tatgcccata tgtaactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
8281 ttgctgaaag catgcggagg aaattctcct tgaagtttcc ctggtgttca aagtaaagga
8341 gtttgcacca gacgcacctc tgttcactgg tccggcgtat taaaacacga tacattgtta
8401 ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac
8461 gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa
8521 atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa
8581 taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa
8641 tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc
8701 gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc
8761 ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa
8821 tagagcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc
8881 gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg
8941 cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat
9001 tgcgatcgca taacttcgta tagcatacat tatacgaagt tataagctcg gaacgctgcg
9061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
9121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
9181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc tgacgagcat
9241 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
9301 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
9361 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
9421 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
9481 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
9541 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
9601 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
9661 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
9721 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
9781 agaaaaaaag gatctcaaga agatcctttg ttaccaatgc ttaatcagtg aggcacctat
9841 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
9901 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
9961 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
10021 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
10081 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
10141 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
10201 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
10261 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
10321 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
10381 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
10441 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
10501 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
10561 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
10621 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
10681 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgtccgcgc gtt
//
