出品公司: | Addgene |
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载体名称: | PX392 或 PX392 Azospirillum B510 Cas9 |
质粒类型: | CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 10700 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
载体标签: | NLS (C ter);HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞(系): | 昆虫细胞 |
备注: | -- |
产品目录号: | #68329 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
买家导航
PX392载体质粒基本信息
PX392质粒图谱载体图谱和PX392载体序列质粒序列多克隆位点信息
PX392质粒载体简介
PX392质粒序列载体序列
LOCUS PX392 Azospirillum B510 Cas9 10700 bp DNA SYN DEFINITION PX392 Azospirillum B510 Cas9 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10700 /organism="PX392 Azospirillum B510 Cas9" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 3" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1023..1587 /label="CMV_immearly_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1090..1377 /label="CAG_enhancer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1544..1564 /label="CMV_fwd_primer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1615..1633 /label="T7_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1633..1656 /label="lacO" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1772..1796 /label="attB1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" CDS 1822..5391 /label="ORF frame 1" /translation="MYPYDVPDYASPKKKRKVEASMARPAFRAPRREHVNGWTPDPHR ISKPFFILVSWHLLSRVVIDSSSGCFPGTSRDHTDKFAEWECAVQPYRLSFDLGTNSI GWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLARQMRRRRDRYLTRRT RLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGRALFHLNQRRGY KPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRARLAGKGK EAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPVGRC TLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPK AGRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTA LPLEEQDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVA ELLPVLERETRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLER HVAFGTGNPADPEEKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLK RSAEDRRREDKRQADNQKRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPY SGETISMRMLLSEQVDIDHILPFSVSLDDSAANKVVCLREANRIKRNRSPWEAFGHDS ERWAGILARAEALPKNKRWRFAPDALEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVC PKVRVSPGRLTALLRRRWGIDAILAEADGPPPEVPAETLDPSPAEKNRADHRHHALDA VVIGCIDRSMVQRVQLAAASAEREAAAREDNIRRVLEGFKEEPWDGFRAELERRARTI VVSHRPEHGIGGALHKETAYGPVDPPEEGFNLVVRKPIDGLSKDEINSVRDPRLRRAL IDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLKKESNPIRVEHGGNPSGP RSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGADGAAAPPRLGDGE RFLMRLHKGDCLKLEHKGRVRVMQVVKLEPSSNSVVVVEPHQVKTDRSKHVKISCDQL RARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS*" misc_feature 1825..1851 /label="HA" /translation="MYPYDVPDYASPKKKRKVEASMARPAFRAPRREHVNGWTPDPHR ISKPFFILVSWHLLSRVVIDSSSGCFPGTSRDHTDKFAEWECAVQPYRLSFDLGTNSI GWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLARQMRRRRDRYLTRRT RLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGRALFHLNQRRGY KPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRARLAGKGK EAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPVGRC TLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPK AGRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTA LPLEEQDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVA ELLPVLERETRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLER HVAFGTGNPADPEEKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLK RSAEDRRREDKRQADNQKRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPY SGETISMRMLLSEQVDIDHILPFSVSLDDSAANKVVCLREANRIKRNRSPWEAFGHDS ERWAGILARAEALPKNKRWRFAPDALEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVC PKVRVSPGRLTALLRRRWGIDAILAEADGPPPEVPAETLDPSPAEKNRADHRHHALDA VVIGCIDRSMVQRVQLAAASAEREAAAREDNIRRVLEGFKEEPWDGFRAELERRARTI VVSHRPEHGIGGALHKETAYGPVDPPEEGFNLVVRKPIDGLSKDEINSVRDPRLRRAL IDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLKKESNPIRVEHGGNPSGP RSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGADGAAAPPRLGDGE RFLMRLHKGDCLKLEHKGRVRVMQVVKLEPSSNSVVVVEPHQVKTDRSKHVKISCDQL RARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS*" misc_feature 1855..1875 /label="SV40 NLS" /translation="MYPYDVPDYASPKKKRKVEASMARPAFRAPRREHVNGWTPDPHR ISKPFFILVSWHLLSRVVIDSSSGCFPGTSRDHTDKFAEWECAVQPYRLSFDLGTNSI GWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLARQMRRRRDRYLTRRT RLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGRALFHLNQRRGY KPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRARLAGKGK EAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPVGRC TLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPK AGRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTA LPLEEQDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVA ELLPVLERETRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLER HVAFGTGNPADPEEKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLK RSAEDRRREDKRQADNQKRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPY SGETISMRMLLSEQVDIDHILPFSVSLDDSAANKVVCLREANRIKRNRSPWEAFGHDS ERWAGILARAEALPKNKRWRFAPDALEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVC PKVRVSPGRLTALLRRRWGIDAILAEADGPPPEVPAETLDPSPAEKNRADHRHHALDA VVIGCIDRSMVQRVQLAAASAEREAAAREDNIRRVLEGFKEEPWDGFRAELERRARTI VVSHRPEHGIGGALHKETAYGPVDPPEEGFNLVVRKPIDGLSKDEINSVRDPRLRRAL IDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLKKESNPIRVEHGGNPSGP RSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGADGAAAPPRLGDGE RFLMRLHKGDCLKLEHKGRVRVMQVVKLEPSSNSVVVVEPHQVKTDRSKHVKISCDQL RARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS*" misc_feature 1861..1875 /label="NLS" /translation="MYPYDVPDYASPKKKRKVEASMARPAFRAPRREHVNGWTPDPHR ISKPFFILVSWHLLSRVVIDSSSGCFPGTSRDHTDKFAEWECAVQPYRLSFDLGTNSI GWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLARQMRRRRDRYLTRRT RLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGRALFHLNQRRGY KPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRARLAGKGK EAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPVGRC TLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPK AGRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTA LPLEEQDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVA ELLPVLERETRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLER HVAFGTGNPADPEEKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLK RSAEDRRREDKRQADNQKRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPY SGETISMRMLLSEQVDIDHILPFSVSLDDSAANKVVCLREANRIKRNRSPWEAFGHDS ERWAGILARAEALPKNKRWRFAPDALEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVC PKVRVSPGRLTALLRRRWGIDAILAEADGPPPEVPAETLDPSPAEKNRADHRHHALDA VVIGCIDRSMVQRVQLAAASAEREAAAREDNIRRVLEGFKEEPWDGFRAELERRARTI VVSHRPEHGIGGALHKETAYGPVDPPEEGFNLVVRKPIDGLSKDEINSVRDPRLRRAL IDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLKKESNPIRVEHGGNPSGP RSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGADGAAAPPRLGDGE RFLMRLHKGDCLKLEHKGRVRVMQVVKLEPSSNSVVVVEPHQVKTDRSKHVKISCDQL RARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS*" CDS complement(2122..2787) /label="ORF frame 3" /translation="MKNSVPQDLASFGGQQIWMVPGLLCPQSVKFGLQHSPCGIEWVC SLFSLPSQSCSQSFSSCFSPCEPSSQSRCSGCSDCCLQPFDSLFHLAGFLIRLCGGAH GFISSALVQVKQGPPDFEGLQPFSCGPLSCQVWIDGHFQALPSCRVCWHQAEAHQSSH QPGPSSQISVPSAPHLSGQSPCHSQRSLVLGIPSVAEDAGPQCSDLPWFSLAIQIQQA PSD*" CDS complement(3454..4377) /label="ORF frame 3" /translation="MVPVVCSVLLSWGRVQCLCGHFWWRSICFCQNGVDAPSPSQQCS QSAWAHPHLRTDAPQILHGQATQMPCIIQVSCSQASLSFQFLQCVRSKAPPFVLGQSF CSCQNPCPSLTVMAEGLPGRSIPLDSVGLPQTDHLVCSGIVQGHAEWQNVIDVHLLAQ QHSHTNCLPRVGAAPIFHWALFLPQPQLQQVSWGWALSQTQDQSLAFLISFLVIGLPL VLSPSIFGRPFQVPCQLNHDFLRAAVPGQNCIHQMPQLVQSDVHSGIGDSSHPLFLRI CGVSCPKSDMSLQHGSIVGQCVQQSSFPAEV*" CDS complement(3921..4562) /label="ORF frame 1" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" misc_feature complement(5424..5441) /label="Sp6_primer" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" misc_feature 5446..5470 /label="attB2" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" terminator 5482..5948 /label="hGH_PA_terminator" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" terminator 5954..5989 /label="T7_terminator" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" rep_origin 6106..6412 /label="f1_origin" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" misc_feature complement(6526..6546) /label="pBABE_3_primer" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" misc_feature complement(6532..6747) /label="SV40_enhancer" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" promoter 6544..6812 /label="SV40_promoter" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" rep_origin 6711..6788 /label="SV40_origin" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" misc_feature 6773..6792 /label="SV40pro_F_primer" /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*" CDS 6927..7721 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 6930..7718 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(7236..7772) /label="ORF frame 1" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7898..8017 /label="SV40_PA_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7986..8005 /label="EBV_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 8208..8243 /label="T7_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature complement(8266..8283) /label="Baculovirs_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 8273..8350 /label="3_AcPH" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" CDS complement(8380..8997) /label="ORF frame 3" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" misc_feature 8977..9010 /label="loxP" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" gene complement(9778..10638) /label="Ampicillin" /gene="Ampicillin" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" CDS complement(9778..10638) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag 1861 aaaaagcgca aggtcgaagc gtccatggca agacctgcat ttcgggcacc tcggagagaa 1921 cacgtcaacg gctggacccc tgacccacat cggattagca aacccttttt catcctggtg 1981 agctggcacc tgctgtcccg ggtggtcatt gacagctcct ctggatgctt cccaggcacc 2041 agccgggacc acaccgacaa gtttgccgag tgggaatgtg ctgtgcagcc ctacaggctg 2101 agtttcgacc tggggaccaa ctcaatcgga tggggcctgc tgaatctgga tcgccaggga 2161 aaaccaaggg agatccgagc actggggtcc cgcatcttca gcgacggacg ggatccccag 2221 gacaaggctt ctctggctgt ggcacggaga ctggccagac agatgaggcg ccgacgggac 2281 agatatctga ctagaaggac caggctgatg ggagctctgg tgcgcttcgg cctgatgcca 2341 gcagaccctg cagctaggaa gcgcctggaa gtggccgtcg atccatacct ggcacgagag 2401 cgggccacaa gagaaaggct ggagcccttc gaaatcggga gggccctgtt tcacctgaac 2461 cagcgccgag gatataaacc cgtgcgcacc gccacaaagc ctgatgagga agccggcaag 2521 gtgaaagagg ctgtcgaaag gctggaggca gcaatcgctg cagccggagc acctactctg 2581 ggagcttggt tcgcatggcg aaaaacacga ggagaaactc tgcgagcacg actggctggg 2641 aagggaaaag aggctgcata cccattctat cccgcacgga gaatgctgga ggccgaattt 2701 gacactctgt gggcagagca ggccaggcac catccagatc tgctgaccgc cgaagctcgc 2761 gagatcctgc ggcacagaat ttttcatcag cggcccctga agccacctcc agtgggaaga 2821 tgcactctgt accctgacga tgggagagct cctagggcac tgccaagcgc tcagaggctg 2881 cgcctgttcc aggagctggc cagcctgaga gtgatccacc tggacctgtc cgaacgccct 2941 ctgaccccag ctgagcgaga tcggattgtg gcatttgtcc agggcagacc ccctaaagcc 3001 ggaaggaagc ctggcaaagt gcagaagagc gtcccattcg aaaagctgag ggggctgctg 3061 gagctgccac caggcactgg gttttctctg gagagtgaca aacggcctga actgctgggc 3121 gacgagacag gcgccagaat cgcaccagca ttcggacctg gatggacagc tctgcctctg 3181 gaggaacagg acgccctggt ggaactgctg ctgacagagg cagaaccaga gagggcaatt 3241 gcagctctga ctgcacgatg ggctctggac gaggcaactg cagcaaagct ggctggagca 3301 accctgccag attttcacgg acgatatggc aggcgcgcag tggctgaact gctgcctgtc 3361 ctggaacgcg agacacgagg cgacccagat gggagagtga ggcccatccg gctggacgag 3421 gcagtgaaac tgctgagagg cgggaaggat cactcagact tcagccggga aggagctctg 3481 ctggacgcac tgccctacta tggagccgtg ctggagagac atgtcgcttt tgggacagga 3541 aaccccgcag atcctgagga aaagcgggtg ggaagagtcg ccaatcccac tgtgcacatc 3601 gctctgaacc agctgcggca tctggtgaat gcaattctgg ccaggcacgg ccgccctgag 3661 gaaatcgtga ttgagctggc acgggacctg aaaaggtctg ccgaagatcg acggagagag 3721 gacaagcggc aggccgataa ccagaaaaga aatgaggaac gcaagcgact gatcctgagt 3781 ctgggagagc gcccaacccc acgaaacctg ctgaagctgc ggctgtggga ggaacagggc 3841 ccagtggaaa ataggcgctg cccctactct ggggagacaa ttagtatgag aatgctgctg 3901 agcgagcagg tggacatcga tcacattctg ccattcagcg tgtccctgga cgattccgct 3961 gcaaacaagg tggtctgtct gcgggaggcc aacagaatca agcggaatag atctccctgg 4021 gaggccttcg gccatgacag tgagagatgg gcagggattc tggcacgagc agaagctctg 4081 cccaagaaca aaaggtggcg ctttgctcct gacgcactgg agaaactgga aggagaggga 4141 ggcctgcgag cacgacacct gaatgataca aggcatctga gtcgcctggc cgtggagtat 4201 ctgcggtgcg tctgtcctaa ggtgcgggtg agcccaggcc gactgactgc actgctgcga 4261 cggagatggg gcatcgacgc cattctggca gaagcagatg gacctccacc agaagtgccc 4321 gcagagacac tggacccttc cccagctgag aagaaccgag cagaccaccg gcaccatgcc 4381 ctggatgctg tggtcatcgg ctgtattgat cgctcaatgg tgcagcgagt ccagctggcc 4441 gctgcaagcg cagaaagaga ggccgctgca agggaggaca atatcaggcg cgtgctggag 4501 ggattcaaag aggaaccttg ggatggcttt agagctgaac tggagcgacg ggcacggacc 4561 atcgtggtga gccacagacc agaacatggg attgggggag ccctgcataa ggagacagct 4621 tacgggcctg tggaccctcc agaggaagga ttcaacctgg tggtcaggaa accaatcgac 4681 ggcctgtcaa aggatgagat taatagcgtg cgggaccccc ggctgagaag ggcactgatc 4741 gatcgcctgg ccattcgccg acgggatgct aacgaccctg ctaccgcact ggccaaagca 4801 gctgaggatc tggcagcaca gccagcctcc cgcggcatca gaagggtgcg ggtcctgaag 4861 aaagaatcta accccattag ggtggagcac ggcgggaatc caagtggacc ccgctcagga 4921 ggcccttttc ataagctgct gctggcagga gaggtgcacc atgtggacgt cgcactgcga 4981 gcagatggcc gccgatgggt gggacactgg gtcacactgt tcgaggcaca tgggggacgg 5041 ggagcagacg gagctgcagc cccacctaga ctgggcgatg gggaaagatt tctgatgagg 5101 ctgcacaagg gagactgcct gaaactggag cataagggca gagtgagggt catgcaggtg 5161 gtcaaactgg aacctagttc aaatagcgtg gtcgtggtcg agccacacca ggtgaaaacc 5221 gacagatcca aacatgtcaa gatctcttgt gatcagctgc gcgctcgagg agcacggaga 5281 gtgaccgtcg atccactggg acgggtgaga gtccacgccc caggagctag ggtgggaatc 5341 ggaggggacg ccggacgaac cgctatggaa cccgcagagg atattagcta agaattcggt 5401 accgagctca agcttgcggc cgcctatagt gtcacctaaa tcgtaaccca gctttcttgt 5461 acaaagtggt cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag 5521 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg 5581 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag 5641 ttgggatgac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg 5701 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc 5761 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt 5821 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga 5881 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc 5941 ctgtccttct agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgtcta 6001 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 6061 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 6121 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 6181 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 6241 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 6301 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 6361 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 6421 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 6481 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 6541 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 6601 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 6661 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 6721 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 6781 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 6841 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 6901 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 6961 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 7021 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 7081 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 7141 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 7201 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 7261 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 7321 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 7381 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 7441 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 7501 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 7561 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 7621 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 7681 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 7741 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 7801 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 7861 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 7921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 7981 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgttcat gaagcccctt 8041 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 8101 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 8161 gagtatttgg tttagagttt ggcaacatat gcccatatgt aactagcata accccttggg 8221 gcctctaaac gggtcttgag gggttttttg ctgaaagcat gcggaggaaa ttctccttga 8281 agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt tcactggtcc 8341 ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag attctgtgcg 8401 ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt 8461 agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa 8521 tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt 8581 ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa 8641 atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg 8701 ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta 8761 caattgactc gacgtaaaca cgttaaatag agcttggaca tatttaacat cgggcgtgtt 8821 agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga 8881 agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat 8941 caaatttgta gttgagcttt ttggaattgc gatcgcataa cttcgtatag catacattat 9001 acgaagttat aagctcggaa cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 9061 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 9121 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 9181 ccataggctc cgccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 9241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 9301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 9361 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 9421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 9481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 9541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 9601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 9661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 9721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgtta 9781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 10021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 10081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 10141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 10201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 10261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 10321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 10381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 10441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 10501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 10561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 10621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 10681 ttgtctcatg tccgcgcgtt //