PX392

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: PX392 或 PX392 Azospirillum B510 Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 10700 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68329
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX392载体图谱



载体简介

载体序列

LOCUS       PX392 Azospirillum B510 Cas9	10700 bp 	DNA	SYN
DEFINITION  PX392 Azospirillum B510 Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="PX392 Azospirillum B510 Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5391
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMARPAFRAPRREHVNGWTPDPHR
                     ISKPFFILVSWHLLSRVVIDSSSGCFPGTSRDHTDKFAEWECAVQPYRLSFDLGTNSI
                     GWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLARQMRRRRDRYLTRRT
                     RLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGRALFHLNQRRGY
                     KPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRARLAGKGK
                     EAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPVGRC
                     TLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPK
                     AGRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTA
                     LPLEEQDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVA
                     ELLPVLERETRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLER
                     HVAFGTGNPADPEEKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLK
                     RSAEDRRREDKRQADNQKRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPY
                     SGETISMRMLLSEQVDIDHILPFSVSLDDSAANKVVCLREANRIKRNRSPWEAFGHDS
                     ERWAGILARAEALPKNKRWRFAPDALEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVC
                     PKVRVSPGRLTALLRRRWGIDAILAEADGPPPEVPAETLDPSPAEKNRADHRHHALDA
                     VVIGCIDRSMVQRVQLAAASAEREAAAREDNIRRVLEGFKEEPWDGFRAELERRARTI
                     VVSHRPEHGIGGALHKETAYGPVDPPEEGFNLVVRKPIDGLSKDEINSVRDPRLRRAL
                     IDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLKKESNPIRVEHGGNPSGP
                     RSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGADGAAAPPRLGDGE
                     RFLMRLHKGDCLKLEHKGRVRVMQVVKLEPSSNSVVVVEPHQVKTDRSKHVKISCDQL
                     RARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMARPAFRAPRREHVNGWTPDPHR
                     ISKPFFILVSWHLLSRVVIDSSSGCFPGTSRDHTDKFAEWECAVQPYRLSFDLGTNSI
                     GWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLARQMRRRRDRYLTRRT
                     RLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGRALFHLNQRRGY
                     KPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRARLAGKGK
                     EAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPVGRC
                     TLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPK
                     AGRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTA
                     LPLEEQDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVA
                     ELLPVLERETRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLER
                     HVAFGTGNPADPEEKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLK
                     RSAEDRRREDKRQADNQKRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPY
                     SGETISMRMLLSEQVDIDHILPFSVSLDDSAANKVVCLREANRIKRNRSPWEAFGHDS
                     ERWAGILARAEALPKNKRWRFAPDALEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVC
                     PKVRVSPGRLTALLRRRWGIDAILAEADGPPPEVPAETLDPSPAEKNRADHRHHALDA
                     VVIGCIDRSMVQRVQLAAASAEREAAAREDNIRRVLEGFKEEPWDGFRAELERRARTI
                     VVSHRPEHGIGGALHKETAYGPVDPPEEGFNLVVRKPIDGLSKDEINSVRDPRLRRAL
                     IDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLKKESNPIRVEHGGNPSGP
                     RSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGADGAAAPPRLGDGE
                     RFLMRLHKGDCLKLEHKGRVRVMQVVKLEPSSNSVVVVEPHQVKTDRSKHVKISCDQL
                     RARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMARPAFRAPRREHVNGWTPDPHR
                     ISKPFFILVSWHLLSRVVIDSSSGCFPGTSRDHTDKFAEWECAVQPYRLSFDLGTNSI
                     GWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLARQMRRRRDRYLTRRT
                     RLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGRALFHLNQRRGY
                     KPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRARLAGKGK
                     EAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPVGRC
                     TLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPK
                     AGRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTA
                     LPLEEQDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVA
                     ELLPVLERETRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLER
                     HVAFGTGNPADPEEKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLK
                     RSAEDRRREDKRQADNQKRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPY
                     SGETISMRMLLSEQVDIDHILPFSVSLDDSAANKVVCLREANRIKRNRSPWEAFGHDS
                     ERWAGILARAEALPKNKRWRFAPDALEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVC
                     PKVRVSPGRLTALLRRRWGIDAILAEADGPPPEVPAETLDPSPAEKNRADHRHHALDA
                     VVIGCIDRSMVQRVQLAAASAEREAAAREDNIRRVLEGFKEEPWDGFRAELERRARTI
                     VVSHRPEHGIGGALHKETAYGPVDPPEEGFNLVVRKPIDGLSKDEINSVRDPRLRRAL
                     IDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLKKESNPIRVEHGGNPSGP
                     RSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGADGAAAPPRLGDGE
                     RFLMRLHKGDCLKLEHKGRVRVMQVVKLEPSSNSVVVVEPHQVKTDRSKHVKISCDQL
                     RARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMARPAFRAPRREHVNGWTPDPHR
                     ISKPFFILVSWHLLSRVVIDSSSGCFPGTSRDHTDKFAEWECAVQPYRLSFDLGTNSI
                     GWGLLNLDRQGKPREIRALGSRIFSDGRDPQDKASLAVARRLARQMRRRRDRYLTRRT
                     RLMGALVRFGLMPADPAARKRLEVAVDPYLARERATRERLEPFEIGRALFHLNQRRGY
                     KPVRTATKPDEEAGKVKEAVERLEAAIAAAGAPTLGAWFAWRKTRGETLRARLAGKGK
                     EAAYPFYPARRMLEAEFDTLWAEQARHHPDLLTAEAREILRHRIFHQRPLKPPPVGRC
                     TLYPDDGRAPRALPSAQRLRLFQELASLRVIHLDLSERPLTPAERDRIVAFVQGRPPK
                     AGRKPGKVQKSVPFEKLRGLLELPPGTGFSLESDKRPELLGDETGARIAPAFGPGWTA
                     LPLEEQDALVELLLTEAEPERAIAALTARWALDEATAAKLAGATLPDFHGRYGRRAVA
                     ELLPVLERETRGDPDGRVRPIRLDEAVKLLRGGKDHSDFSREGALLDALPYYGAVLER
                     HVAFGTGNPADPEEKRVGRVANPTVHIALNQLRHLVNAILARHGRPEEIVIELARDLK
                     RSAEDRRREDKRQADNQKRNEERKRLILSLGERPTPRNLLKLRLWEEQGPVENRRCPY
                     SGETISMRMLLSEQVDIDHILPFSVSLDDSAANKVVCLREANRIKRNRSPWEAFGHDS
                     ERWAGILARAEALPKNKRWRFAPDALEKLEGEGGLRARHLNDTRHLSRLAVEYLRCVC
                     PKVRVSPGRLTALLRRRWGIDAILAEADGPPPEVPAETLDPSPAEKNRADHRHHALDA
                     VVIGCIDRSMVQRVQLAAASAEREAAAREDNIRRVLEGFKEEPWDGFRAELERRARTI
                     VVSHRPEHGIGGALHKETAYGPVDPPEEGFNLVVRKPIDGLSKDEINSVRDPRLRRAL
                     IDRLAIRRRDANDPATALAKAAEDLAAQPASRGIRRVRVLKKESNPIRVEHGGNPSGP
                     RSGGPFHKLLLAGEVHHVDVALRADGRRWVGHWVTLFEAHGGRGADGAAAPPRLGDGE
                     RFLMRLHKGDCLKLEHKGRVRVMQVVKLEPSSNSVVVVEPHQVKTDRSKHVKISCDQL
                     RARGARRVTVDPLGRVRVHAPGARVGIGGDAGRTAMEPAEDIS*"
     CDS             complement(2122..2787)
                     /label="ORF frame 3"
                     /translation="MKNSVPQDLASFGGQQIWMVPGLLCPQSVKFGLQHSPCGIEWVC
                     SLFSLPSQSCSQSFSSCFSPCEPSSQSRCSGCSDCCLQPFDSLFHLAGFLIRLCGGAH
                     GFISSALVQVKQGPPDFEGLQPFSCGPLSCQVWIDGHFQALPSCRVCWHQAEAHQSSH
                     QPGPSSQISVPSAPHLSGQSPCHSQRSLVLGIPSVAEDAGPQCSDLPWFSLAIQIQQA
                     PSD*"
     CDS             complement(3454..4377)
                     /label="ORF frame 3"
                     /translation="MVPVVCSVLLSWGRVQCLCGHFWWRSICFCQNGVDAPSPSQQCS
                     QSAWAHPHLRTDAPQILHGQATQMPCIIQVSCSQASLSFQFLQCVRSKAPPFVLGQSF
                     CSCQNPCPSLTVMAEGLPGRSIPLDSVGLPQTDHLVCSGIVQGHAEWQNVIDVHLLAQ
                     QHSHTNCLPRVGAAPIFHWALFLPQPQLQQVSWGWALSQTQDQSLAFLISFLVIGLPL
                     VLSPSIFGRPFQVPCQLNHDFLRAAVPGQNCIHQMPQLVQSDVHSGIGDSSHPLFLRI
                     CGVSCPKSDMSLQHGSIVGQCVQQSSFPAEV*"
     CDS             complement(3921..4562)
                     /label="ORF frame 1"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     misc_feature    complement(5424..5441)
                     /label="Sp6_primer"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     misc_feature    5446..5470
                     /label="attB2"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     terminator      5482..5948
                     /label="hGH_PA_terminator"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     terminator      5954..5989
                     /label="T7_terminator"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     rep_origin      6106..6412
                     /label="f1_origin"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     misc_feature    complement(6526..6546)
                     /label="pBABE_3_primer"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     misc_feature    complement(6532..6747)
                     /label="SV40_enhancer"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     promoter        6544..6812
                     /label="SV40_promoter"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     rep_origin      6711..6788
                     /label="SV40_origin"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     misc_feature    6773..6792
                     /label="SV40pro_F_primer"
                     /translation="MVRARRSSSALKPSQGSSLNPSSTRLILSSLAAASLSALAAASW
                     TRCTIERSIQPMTTASRAWCRWSARFFSAGEGSSVSAGTSGGGPSASARMASMPHLRR
                     SSAVSRPGLTRTLGQTHRRYSTARRLRCLVSFRCRARRPPSPSSFSSASGAKRHLLFL
                     GRASARARIPAHLSLSWPKASQGDLFRLILLASRRQTTLFAAESSRDTLNGRM*"
     CDS             6927..7721
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6930..7718
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7236..7772)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7898..8017
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7986..8005
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8208..8243
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8266..8283)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8273..8350
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8380..8997)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8977..9010
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9778..10638)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9778..10638)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatggca agacctgcat ttcgggcacc tcggagagaa
 1921 cacgtcaacg gctggacccc tgacccacat cggattagca aacccttttt catcctggtg
 1981 agctggcacc tgctgtcccg ggtggtcatt gacagctcct ctggatgctt cccaggcacc
 2041 agccgggacc acaccgacaa gtttgccgag tgggaatgtg ctgtgcagcc ctacaggctg
 2101 agtttcgacc tggggaccaa ctcaatcgga tggggcctgc tgaatctgga tcgccaggga
 2161 aaaccaaggg agatccgagc actggggtcc cgcatcttca gcgacggacg ggatccccag
 2221 gacaaggctt ctctggctgt ggcacggaga ctggccagac agatgaggcg ccgacgggac
 2281 agatatctga ctagaaggac caggctgatg ggagctctgg tgcgcttcgg cctgatgcca
 2341 gcagaccctg cagctaggaa gcgcctggaa gtggccgtcg atccatacct ggcacgagag
 2401 cgggccacaa gagaaaggct ggagcccttc gaaatcggga gggccctgtt tcacctgaac
 2461 cagcgccgag gatataaacc cgtgcgcacc gccacaaagc ctgatgagga agccggcaag
 2521 gtgaaagagg ctgtcgaaag gctggaggca gcaatcgctg cagccggagc acctactctg
 2581 ggagcttggt tcgcatggcg aaaaacacga ggagaaactc tgcgagcacg actggctggg
 2641 aagggaaaag aggctgcata cccattctat cccgcacgga gaatgctgga ggccgaattt
 2701 gacactctgt gggcagagca ggccaggcac catccagatc tgctgaccgc cgaagctcgc
 2761 gagatcctgc ggcacagaat ttttcatcag cggcccctga agccacctcc agtgggaaga
 2821 tgcactctgt accctgacga tgggagagct cctagggcac tgccaagcgc tcagaggctg
 2881 cgcctgttcc aggagctggc cagcctgaga gtgatccacc tggacctgtc cgaacgccct
 2941 ctgaccccag ctgagcgaga tcggattgtg gcatttgtcc agggcagacc ccctaaagcc
 3001 ggaaggaagc ctggcaaagt gcagaagagc gtcccattcg aaaagctgag ggggctgctg
 3061 gagctgccac caggcactgg gttttctctg gagagtgaca aacggcctga actgctgggc
 3121 gacgagacag gcgccagaat cgcaccagca ttcggacctg gatggacagc tctgcctctg
 3181 gaggaacagg acgccctggt ggaactgctg ctgacagagg cagaaccaga gagggcaatt
 3241 gcagctctga ctgcacgatg ggctctggac gaggcaactg cagcaaagct ggctggagca
 3301 accctgccag attttcacgg acgatatggc aggcgcgcag tggctgaact gctgcctgtc
 3361 ctggaacgcg agacacgagg cgacccagat gggagagtga ggcccatccg gctggacgag
 3421 gcagtgaaac tgctgagagg cgggaaggat cactcagact tcagccggga aggagctctg
 3481 ctggacgcac tgccctacta tggagccgtg ctggagagac atgtcgcttt tgggacagga
 3541 aaccccgcag atcctgagga aaagcgggtg ggaagagtcg ccaatcccac tgtgcacatc
 3601 gctctgaacc agctgcggca tctggtgaat gcaattctgg ccaggcacgg ccgccctgag
 3661 gaaatcgtga ttgagctggc acgggacctg aaaaggtctg ccgaagatcg acggagagag
 3721 gacaagcggc aggccgataa ccagaaaaga aatgaggaac gcaagcgact gatcctgagt
 3781 ctgggagagc gcccaacccc acgaaacctg ctgaagctgc ggctgtggga ggaacagggc
 3841 ccagtggaaa ataggcgctg cccctactct ggggagacaa ttagtatgag aatgctgctg
 3901 agcgagcagg tggacatcga tcacattctg ccattcagcg tgtccctgga cgattccgct
 3961 gcaaacaagg tggtctgtct gcgggaggcc aacagaatca agcggaatag atctccctgg
 4021 gaggccttcg gccatgacag tgagagatgg gcagggattc tggcacgagc agaagctctg
 4081 cccaagaaca aaaggtggcg ctttgctcct gacgcactgg agaaactgga aggagaggga
 4141 ggcctgcgag cacgacacct gaatgataca aggcatctga gtcgcctggc cgtggagtat
 4201 ctgcggtgcg tctgtcctaa ggtgcgggtg agcccaggcc gactgactgc actgctgcga
 4261 cggagatggg gcatcgacgc cattctggca gaagcagatg gacctccacc agaagtgccc
 4321 gcagagacac tggacccttc cccagctgag aagaaccgag cagaccaccg gcaccatgcc
 4381 ctggatgctg tggtcatcgg ctgtattgat cgctcaatgg tgcagcgagt ccagctggcc
 4441 gctgcaagcg cagaaagaga ggccgctgca agggaggaca atatcaggcg cgtgctggag
 4501 ggattcaaag aggaaccttg ggatggcttt agagctgaac tggagcgacg ggcacggacc
 4561 atcgtggtga gccacagacc agaacatggg attgggggag ccctgcataa ggagacagct
 4621 tacgggcctg tggaccctcc agaggaagga ttcaacctgg tggtcaggaa accaatcgac
 4681 ggcctgtcaa aggatgagat taatagcgtg cgggaccccc ggctgagaag ggcactgatc
 4741 gatcgcctgg ccattcgccg acgggatgct aacgaccctg ctaccgcact ggccaaagca
 4801 gctgaggatc tggcagcaca gccagcctcc cgcggcatca gaagggtgcg ggtcctgaag
 4861 aaagaatcta accccattag ggtggagcac ggcgggaatc caagtggacc ccgctcagga
 4921 ggcccttttc ataagctgct gctggcagga gaggtgcacc atgtggacgt cgcactgcga
 4981 gcagatggcc gccgatgggt gggacactgg gtcacactgt tcgaggcaca tgggggacgg
 5041 ggagcagacg gagctgcagc cccacctaga ctgggcgatg gggaaagatt tctgatgagg
 5101 ctgcacaagg gagactgcct gaaactggag cataagggca gagtgagggt catgcaggtg
 5161 gtcaaactgg aacctagttc aaatagcgtg gtcgtggtcg agccacacca ggtgaaaacc
 5221 gacagatcca aacatgtcaa gatctcttgt gatcagctgc gcgctcgagg agcacggaga
 5281 gtgaccgtcg atccactggg acgggtgaga gtccacgccc caggagctag ggtgggaatc
 5341 ggaggggacg ccggacgaac cgctatggaa cccgcagagg atattagcta agaattcggt
 5401 accgagctca agcttgcggc cgcctatagt gtcacctaaa tcgtaaccca gctttcttgt
 5461 acaaagtggt cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
 5521 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
 5581 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
 5641 ttgggatgac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
 5701 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
 5761 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
 5821 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
 5881 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
 5941 ctgtccttct agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgtcta
 6001 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
 6061 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
 6121 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
 6181 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
 6241 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
 6301 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
 6361 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
 6421 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
 6481 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
 6541 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
 6601 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
 6661 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
 6721 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
 6781 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
 6841 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
 6901 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
 6961 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
 7021 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
 7081 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
 7141 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
 7201 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
 7261 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
 7321 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
 7381 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
 7441 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
 7501 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
 7561 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
 7621 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
 7681 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
 7741 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
 7801 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
 7861 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
 7921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
 7981 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgttcat gaagcccctt
 8041 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
 8101 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
 8161 gagtatttgg tttagagttt ggcaacatat gcccatatgt aactagcata accccttggg
 8221 gcctctaaac gggtcttgag gggttttttg ctgaaagcat gcggaggaaa ttctccttga
 8281 agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt tcactggtcc
 8341 ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag attctgtgcg
 8401 ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt
 8461 agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa
 8521 tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt
 8581 ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa
 8641 atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg
 8701 ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta
 8761 caattgactc gacgtaaaca cgttaaatag agcttggaca tatttaacat cgggcgtgtt
 8821 agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga
 8881 agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat
 8941 caaatttgta gttgagcttt ttggaattgc gatcgcataa cttcgtatag catacattat
 9001 acgaagttat aagctcggaa cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
 9061 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
 9121 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
 9181 ccataggctc cgccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
 9241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
 9301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
 9361 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
 9421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
 9481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
 9541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
 9601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
 9661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
 9721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgtta
 9781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
 9841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
 9901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
 9961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
10021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
10081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
10141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
10201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
10261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
10321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
10381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
10441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
10501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
10561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
10621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
10681 ttgtctcatg tccgcgcgtt
//

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