| 质粒名称: | E1b-GFP-Tol2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37845 |
| 存储实验室: | Nadav Ahituv |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 斑马鱼增强子检测载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | E1b |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | GATGCGGGAAGAGGTGTATTAGT |
| 3' 测序引物 | AGTTCTCAGGATCGGTCGAAAT |
|
文献引用方法 |
|
| 材料和方法部分: | E1b-GFP-Tol2 was a gift from Nadav Ahituv (Addgene plasmid # 37845 ; http://n2t.net/addgene:37845 ; RRID:Addgene_37845) |
| 参考文献部分: | Coding exons function as tissue-specific enhancers of nearby genes. Birnbaum RY, Clowney EJ, Agamy O, Kim MJ, Zhao J, Yamanaka T, Pappalardo Z, Clarke SL, Wenger AM, Nguyen L, Gurrieri F, Everman DB, Schwartz CE, Birk OS, Bejerano G, Lomvardas S, Ahituv N. Genome Res. 2012 Jun;22(6):1059-68. Epub 2012 Mar 22. 10.1101/gr.133546.111 PubMed 22442009 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS E1b-GFP-Tol2 7602 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS E1b-GFP-Tol2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7602)
AUTHORS Birnbaum RY, Clowney EJ, Agamy O, Kim MJ, Zhao J, Yamanaka T,
Pappalardo Z, Clarke SL, Wenger AM, Nguyen L, Gurrieri F, Everman
DB, Schwartz CE, Birk OS, Bejerano G, Lomvardas S, Ahituv N
TITLE Coding exons function as tissue-specific enhancers of nearby genes.
JOURNAL Genome Res. 2012 Jun;22(6):1059-68. Epub 2012 Mar 22.
PUBMED 22442009
REFERENCE 2 (bases 1 to 7602)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7602
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 49..68
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(287..391)
/gene="bla"
/label=AmpR promoter
rep_origin complement(417..872)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(554..575)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 766..785
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 999..1021
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 1013..1030
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 1014..1030
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1040..1059
/label=T7
/note="T7 promoter, forward primer"
promoter 1040..1058
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
intron 3555..4127
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
regulatory 4201..4210
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4207..4926
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4252..4273)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4513..4532)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4860..4881
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 4971..5105
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4995..5014)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5049..5068
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(5775..5791)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5775..5791)
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(5828..5848)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(5828..5846)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5867..5883)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5867..5883)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5880..5902)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5891..5907
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5915..5945)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5960..5981
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6098..6115)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6269..6857)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6349..6368)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7028..286)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
61 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
421 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
481 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
541 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
601 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
661 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
721 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
781 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
841 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
901 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
961 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
1021 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta ccaaatagta
1081 ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt tgccattttc
1141 tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct tgaataaatg
1201 ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca ataatatgta
1261 atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga gaactggacc
1321 gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca aaatgaagtt
1381 aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga cgttgccaat
1441 aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc aacccctcta
1501 accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc gggctgcaca
1561 aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt aaggcatctg
1621 gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta ttacagaaat
1681 tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca ctggagcatt
1741 catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca ctcattacag
1801 ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt gattgattga
1861 ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt gattggtagt
1921 caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca tacataaaaa
1981 actggggggg gggggataca acaacacact taagtcacat ttgcctacgt aaagaaaagt
2041 aaagaaaatc aatagctata ttttacatct cctttttttg ctgtctttta attagccttg
2101 ttttgctgtt atcttgattg aaactgtaac ttcttcacct gcttcttttc tttgtaggtt
2161 ttgccagccc agaaacaggc atggctgtgc aaggccagag caccatgcac aatgcgctgc
2221 atgtcttcat gaacggctca atgtcctcag tccagggctc agccaacgac cccatcttcc
2281 tccttcacca tgctttcatt gacaggtaac aaacacgtca tgacattaga ctgcacagtt
2341 tttgacaaag ttcatacaat ctgttgttta tagctgctac aattagtgaa gtttgtgaat
2401 gtacttggat gagcagcgaa agatcaattg agatcaattg ttagagtttg gttgccctgc
2461 agagcaaaga acaaaaaata atctggtggc tttactgcgt gaggttatta ttggtggaat
2521 agaaacacaa aacataattg catttatttg tttaattttt tatcttatct taactttcat
2581 cttgcatatt tgtttcttac atcatttcta gcatctttga gcgctggcta agaactcatc
2641 agcctccccg gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact
2701 acatggtgcc attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc
2761 ttggatacga gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct
2821 ctgatctgca aaagacgtga atatctgttc agacacccat atccactctg ttccacacag
2881 gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc
2941 aagtgaaagt acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct
3001 agaatcttac ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag
3061 gaagtaaaag taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa
3121 aaaacattaa atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat
3181 tatcaaacta taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct
3241 cagatttaac ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt
3301 ggggctgaaa agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag
3361 aggtgtatta gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct
3421 ggtgtctgaa acacaggcca gatgggccct cgagagatct ctcgactcta gagggtatat
3481 aatggatccc atcgcgtctc agcctcactt tgagctcctc cacacgaatt tcgaccgatc
3541 ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa
3601 aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc
3661 ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac
3721 aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct
3781 tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc
3841 tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt
3901 agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct
3961 ggcgtggaaa tattcttatt ggtagaaaca actacaccct ggtcatcatc ctgcctttct
4021 ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac
4081 cgggcccctc tgctaaccat gttcatgcct tcttctcttt cctacagctc ctgggcaacg
4141 tgctggttgt tgtgctgtct catcattttg gcaaagaatt cctcgacgga tccaccggtc
4201 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
4261 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
4321 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
4381 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
4441 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
4501 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
4561 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
4621 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
4681 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
4741 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
4801 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
4861 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
4921 aagtaaagcg gccgccaccg cggtggagct cgaattaatt catcgatgat gatccagaca
4981 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
5041 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
5101 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
5161 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc
5221 agatccgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg actgtgctgc
5281 tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg actgttataa
5341 tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga catgacgtca
5401 cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa cgattttcat
5461 aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat tggtttggta
5521 atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat
5581 aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa
5641 aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt
5701 acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac cccctacctt
5761 cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg agctccagct
5821 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
5881 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
5941 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
6001 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
6061 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
6121 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
6181 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
6241 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
6301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
6361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
6421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
6481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
6541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
6601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
6661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
6721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
6781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
6841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
6901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
6961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
7021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
7081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
7141 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
7201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
7261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
7321 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
7381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
7441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
7501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
7561 gcttttctgt gactggtgag tactcaacca agtcattctg ag
//
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