| 出品公司: | ATCC |
|---|---|
| 载体名称: | YCplac111 |
| 质粒类型: | 酿酒酵母表达载体；中心粒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lacZ |
| 载体大小: | 6112 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | LEU3 |
| 表达菌株: | 酿酒酵母、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | 87587 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS YCplac111 6112 bp DNA SYN
DEFINITION YCplac111
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6112
/organism="YCplac111"
/mol_type="other DNA"
promoter 142..171
/label="lac_promoter"
misc_feature 185..207
/label="M13_pUC_rev_primer"
promoter 206..224
/label="M13_reverse_primer"
misc_feature 287..442
/label="lacZ_a"
promoter complement(290..306)
/label="M13_forward20_primer"
misc_feature complement(299..321)
/label="M13_pUC_fwd_primer"
misc_feature 504..1356
/label="CEN4"
CDS complement(2066..2545)
/label="ORF frame 1"
/translation="MKFIIETTRNYKMEYVHRVELILMFDSLSSIVQLHGDESWQEYQ
EFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDWVGRQ
ESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNAKK*"
gene complement(2589..3683)
/label="LEU2 (variant)"
/gene="LEU2 (variant)"
/translation="MKFIIETTRNYKMEYVHRVELILMFDSLSSIVQLHGDESWQEYQ
EFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDWVGRQ
ESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNAKK*"
CDS complement(2589..3695)
/label="ORF frame 3"
/translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
promoter 4241..4269
/label="AmpR_promoter"
/translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
gene 4311..5171
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
CDS 4311..5171
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 5326..5945
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat
541 tgtcatatta ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat
601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
961 taaaagttca ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa
1021 atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt
1081 aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat
1141 tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga
1201 aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac
1261 agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc
1321 acagttttac agtaaataag tatcacctct tagagttcga tgataagctg tcaaacatga
1381 gaattaattc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg
1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt
1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag
1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac
1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata
1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag
1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag
1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt
1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa
1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta
1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact
2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatcgatg
2461 ataagctgtc aaacatgaga attaattcta ccctatgaac atattccatt ttgtaatttc
2521 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
2581 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg
2641 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
2701 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
2761 gcagacaaga tagtggcgat agggtcaacc ttattctttg gcaaatctgg agcagaaccg
2821 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
2881 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
2941 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
3001 gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt
3061 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
3121 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
3181 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
3241 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
3301 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
3361 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
3421 ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcaacc
3481 ttcttggagg cttccagcgc ctcatctgga agtgggacac ctgtagcatc gatagcagca
3541 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
3601 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
3661 acgatcttct taggggcaga cataggggca gacattagaa tggtatatcc ttgaaatata
3721 tatatatatt gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc
3781 acctattgga aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc
3841 aagcatttag tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcctctcacc
3901 tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg acctctgaaa
3961 ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc tgtgtgttct
4021 cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc atcttacgat
4081 acctgagtat tcccacagtt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
4141 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
4201 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
4261 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
4321 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
4381 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
4441 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
4501 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
4561 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
4621 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
4681 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
4741 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
4801 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
4861 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
4921 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
4981 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
5041 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
5101 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
5161 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
5221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
5281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
5341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
5401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
5461 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
5521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
5581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
5641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
5701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
5761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
5821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
5881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
5941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
6001 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
6061 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
