质粒名称: | pCMV_ABEmax |
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出品公司: | addgene |
目录编号: | 112095 |
存储实验室: | David Liu |
载体骨架基本信息 |
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载体名称: | pCMV |
完整质粒大小: | 8811 bp |
载体类型: | 哺乳细胞表达、CRISPR |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | NEB Stable |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | ABEmax |
物种来源: | H. sapiens (human) |
插入大小: | 5412 bp |
启动子: | CMV |
克隆方法: | 未知 |
5’测序引物 | T7 Forward |
3’测序引物 | BGH-Reverse |
文献引用方法 |
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材料和方法部分: |
pCMV_ABEmax was a gift from David Liu (Addgene plasmid # 112095 ; http://n2t.net/addgene:112095 ; RRID:Addgene_112095)
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参考文献部分: | mproving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A, Liu DR. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. 10.1038/nbt.4172 PubMed 29813047 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV_ABEmax 8811 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION A:T-to-G:C base editing. ACCESSION . VERSION . KEYWORDS pCMV_ABEmax SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8811) AUTHORS Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A, Liu DR TITLE Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. JOURNAL Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. PUBMED 29813047 REFERENCE 2 (bases 1 to 8811) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..8811 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 132..335 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 286..306 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 377..396 /label=T7 /note="T7 promoter, forward primer" promoter 377..395 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 445..465 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1654..5754 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(D10A) /note="generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5797..5817 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5826..5843 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(5866..5883) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5872..6096 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(6167..6183) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6167..6183) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6180..6202) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6191..6207 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6215..6245) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6260..6281 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6398..6415) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6569..7157) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6649..6668) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7328..8188) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7951..7970 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8189..8293) /gene="bla" /label=AmpR promoter primer_bind complement(8372..8391) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 8563..131 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga agtcgagttt 481 agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga 541 gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac 601 aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga 661 ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca 721 tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca 781 cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg 841 aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc 901 gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc 961 gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc 1021 gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac 1081 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgcgatga gagggaggtg 1141 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc 1201 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg 1261 gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg 1321 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac 1381 gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc actaccccgg catgaatcac 1441 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgctatttc 1501 tttcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc caccgactcc 1561 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca 1621 cctgaaagca gcgggggcag cagcgggggg tcagacaaga agtacagcat cggcctggcc 1681 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag 1741 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc 1801 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga 1861 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg 1921 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat 1981 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag 2041 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac 2101 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc 2161 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag 2221 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc 2281 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc 2341 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc 2401 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc 2461 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt 2521 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc 2581 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag 2641 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt 2701 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa 2761 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc 2821 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc 2881 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac 2941 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac 3001 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag 3061 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc 3121 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag 3181 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg 3241 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac 3301 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag 3361 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc 3421 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag 3481 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag 3541 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag 3601 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc 3661 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac 3721 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa 3781 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc 3841 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg 3901 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc 3961 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag 4021 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag 4081 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc 4141 aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac 4201 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg 4261 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag 4321 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa 4381 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac 4441 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc 4501 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc 4561 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac 4621 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac 4681 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc 4741 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt 4801 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg 4861 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc 4921 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc 4981 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag 5041 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa 5101 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa 5161 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg 5221 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg 5281 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc 5341 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag 5401 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc 5461 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg 5521 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc 5581 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc 5641 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag 5701 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgactctggc 5761 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtctaa 5821 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt 5881 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 5941 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 6001 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 6061 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 6121 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 6181 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6241 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 6361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 6421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 6481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 6541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 6601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 6781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 7021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac actcagtgga 7201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 8341 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa 8401 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt 8461 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc 8521 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta 8581 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 8641 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 8701 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 8761 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat c //
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