pKC-4.06

LOCUS       pKC-4.06               9274 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC).
ACCESSION   .
VERSION     .
KEYWORDS    pKC-4.06
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg 
            JR
  TITLE     ICE1 promotes the link between splicing and nonsense-mediated mRNA 
            decay.
  JOURNAL   Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178.
  PUBMED    29528287
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             614..679
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             698..2344
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(761..779)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    2372..2396
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3658..3682)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3716..4338
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3915..3934)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4362..4382)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4380..4677
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     4609..4628
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4731..5525
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4785..4804)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5395..5414
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6177..6311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6214..6233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6268..6287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6316..6335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6317..6335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6349..6365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6349..6365)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6362..6384)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6373..6389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6397..6427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6442..6463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6580..6597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6751..7339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6831..6850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7510..8370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8133..8152
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8371..8475)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8502..8957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8589..8608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8799..8820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9083..9105
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9097..9114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9098..9114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattaacc atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta
      661 caaggatgac gatgacaagc ttgcggccgc gaattcagaa gacgccaaaa acataaagaa
      721 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc
      781 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
      841 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
      901 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
      961 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
     1021 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc
     1081 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat
     1141 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
     1201 atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga
     1261 caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc
     1321 tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa
     1381 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat
     1441 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga
     1501 agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc
     1561 aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt
     1621 acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa
     1681 gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat
     1741 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt
     1801 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga
     1861 actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa
     1921 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga
     1981 cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt
     2041 ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt
     2101 cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca
     2161 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc
     2221 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa
     2281 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc
     2341 cgtggcgaat tcatcgatag atctgatatc aacaagtttg tacaaaaaag caggctccgc
     2401 ggccgccccc ttcaccgtgc atctgactcc tgaggagaag tctgccgtta ctgccctgtg
     2461 gggcaaggtg aacgtggatg aagttggtgg tgaggccctg ggcaggttgg tatcaaggtt
     2521 acaagacagg tttaaggaga ccaatagaaa ctgggcatgt ggagacagag aagactcttg
     2581 ggtttctgat aggcactgac tctctctgcc tattggtcta ttttcccacc cttaggctgc
     2641 tggtggtcta cccttggacc tagaggttct ttgagtcctt tggggatctg tccactcctg
     2701 atgctgttat gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc ggtgccttta
     2761 gtgatggcct ggctcacctg gacaacctca agggcacctt tgccacactg agtgagctgc
     2821 actgtgacaa gctgcacgtg gatcctgaga acttcagggt gagtctgatg ggcacctcct
     2881 gggtttcctt cccctggcta ttctgctcaa ccttcctatc agaaaaaaag gggaagcgat
     2941 tctagggagc agtctccatg actgtgtgtg gagtgttgac aagagttcgg atattttatt
     3001 ctctactcag aattgctgct ccccctcact ctgttctgtg ttgtcatttc ctctttcttt
     3061 ggtaagcttt taatttccag ttgcatttta ctaaattaat taagctggtt atttacttcc
     3121 catcctgata tcagcttccc ctcctccttt cctcccagtc cttctctctc tcctctctct
     3181 ttctctaatc ctttcctttc cctcagttca tttcttcttc tttgatctac gtttgtttgt
     3241 ctttttaaat attgccttgt aacttgctca gaggacaagg aagatatgtc cctgtttctt
     3301 ctcatagctc tcaagaatag tagcataatt ggcttttatg ccagggtgac aggggaagaa
     3361 tatattttac atataaattc tgtttgacat aggattctta taataatttg tcagtagttt
     3421 aaggttgcaa acaaatgtct ttgtaaataa gcctgcagta tctggtattt ttgctctaca
     3481 gttatgttga tggttcttcc atattcccac agctcctggg caatatgatc gtgattgtgc
     3541 tgggccacca cctgggcaag gatttcaccc ccgctgcaca ggctgccttc cagaaggtgg
     3601 tggctggagt ggccactgcc ctggcccaca agtatcacta aaagggtggg cgcgccgacc
     3661 cagctttctt gtacaaagtg gttgatatcg gtaccagtcg actctagagg atcccgggtg
     3721 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     3781 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     3841 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     3901 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     3961 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     4021 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     4081 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     4141 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     4201 aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca
     4261 accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca
     4321 gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt gtcagttagg
     4381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4441 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4501 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4681 aattgatcag cttgggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4741 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4801 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4861 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     4921 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4981 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5041 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5101 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5161 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5221 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5281 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5341 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5401 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5461 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5521 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5581 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5641 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg
     5701 gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt
     5761 ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca
     5821 tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc cggacgggac
     5881 gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcatgagtgt gtcttcccgt
     5941 tttccgcctg aggtcactgc gtggatggag cgctggcgcc tgctgcgcga cggcgagctg
     6001 ctcaccaccc actcgccaag ctggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6061 aggctccgcc gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     6121 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     6181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6301 atgtctggat caattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt
     6361 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6421 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6481 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6541 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7621 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7801 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8101 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8461 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     8521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8941 attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     9001 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     9061 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
     9121 ctgatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
     9181 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
     9241 tatattggct catgtccaat atgaccgcca tgtt
//