| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.51 ; pGL4.51[luc2/CMV/Neo] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6358 bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | EBV-R: GTGGTTTGTCCAAACTCATC |
| 载体标签: | luc2 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 新霉素(Neomycin) |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.51[luc2/CMV/Neo]载体是信号通路报告载体；含ARE应答元件；含荧光 素酶报告基因luc2。 |
| 产品目录号: | E1320 |
| 稳定性: | 瞬表达或稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.51载体基本信息
pGL4.51载体图谱质粒图谱和多克隆位点信息
pGL4.51载体简介
pGL4.51载体简介 The pGL4.51[luc2/CMV/Neo] Vector encodes the luciferase reporter gene luc2 (Photinus pyralis), which has been codon optimized for mammalian expression. This vector is also engineered with fewer consensus regulatory sequences for reduced backgrounds and a decreased risk of anomalous transcription. This vector contains the following features: luc2 reporter gene for expression in mammalian cells; CMV promoter for high translational expression; SV40 late poly(A) signal sequence is positioned downstream of luc2 to provide efficient transcription termination and mRNA polyadenylation; Binding region for RVprimer 3 and RVprimer 4; Synthetic poly(A) signal/transcription start site; Synthetic Neomycin-resistance gene for mammalian cell selection of the plasmid; Plasmid replication origin; Ampr gene for bacterial selection for vector amplification.
pGL4.51载体序列
LOCUS EU921841 6358 bp DNA linear SYN 28-AUG-2008
DEFINITION Firefly luciferase reporter vector pGL4.51[luc2/CMV/Neo], complete
sequence.
ACCESSION EU921841
VERSION EU921841.1 GI:197215836
KEYWORDS .
SOURCE Firefly luciferase reporter vector pGL4.51[luc2/CMV/Neo]
ORGANISM Firefly luciferase reporter vector pGL4.51[luc2/CMV/Neo]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6358)
AUTHORS Brouette,C., Hung,L. and Kopish,K.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6358)
AUTHORS Brouette,C., Hung,L. and Kopish,K.
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2008) SciComm, Promega, 5500 E Cheryl Parkway,
Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..6358
/organism="Firefly luciferase reporter vector
pGL4.51[luc2/CMV/Neo]"
/mol_type="other DNA"
/db_xref="taxon:554673"
regulatory 14..755
/regulatory_class="promoter"
/note="CMV immediate early enhancer/promoter"
gene 859..2511
/gene="luc2"
CDS 859..2511
/gene="luc2"
/note="luminescent reporter protein; derived from Photinus
pyralis"
/codon_start=1
/transl_table=11
/product="firefly luciferase"
/protein_id="ACH53166.1"
/db_xref="GI:197215837"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
misc_feature 2546..2767
/note="SV40 late poly(A) region"
regulatory 2815..3233
/regulatory_class="promoter"
/note="SV40 early enhancer/promoter"
gene 3258..4052
/gene="NeoR"
CDS 3258..4052
/gene="NeoR"
/note="antibiotic resistance protein"
/codon_start=1
/transl_table=11
/product="neomycin phosphotransferase"
/protein_id="ACH53167.1"
/db_xref="GI:197215838"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4077..4125
/note="poly(A) region"
primer_bind complement(4192..4211)
/note="RV primer 4 binding region"
gene 5240..6100
/gene="AmpR"
CDS complement(5240..6100)
/note="confers resistance to ampicillin"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="ACH53168.1"
/db_xref="GI:197215839"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 6205..6358
/note="poly(A) signal/transcription pause region"
primer_bind 6307..6326
/note="RV primer 3 binding region"
ORIGIN
1 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
61 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
121 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
721 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
781 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
841 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
901 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
961 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
1021 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
1081 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
1141 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
1201 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
1261 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
1321 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
1381 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
1441 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
1501 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
1561 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
1621 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
1681 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
1741 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
1801 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
1861 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
1921 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
1981 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
2041 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
2101 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
2161 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
2221 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
2281 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
2341 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
2401 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
2461 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta ataattctag
2521 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
2581 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
2641 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
2701 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
2761 tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc tcttccgctg atctgcgcag
2821 caccatggcc tgaaataacc tctgaaagag gaacttggtt agctaccttc tgaggcggaa
2881 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
2941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
3001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
3061 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
3121 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
3181 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcgattctt
3241 ctgacactag cgccaccatg atcgaacaag acggcctcca tgctggcagt cccgcagctt
3301 gggtcgaacg cttgttcggg tacgactggg cccagcagac catcggatgt agcgatgcgg
3361 ccgtgttccg tctaagcgct caaggccggc ccgtgctgtt cgtgaagacc gacctgagcg
3421 gcgccctgaa cgagcttcaa gacgaggctg cccgcctgag ctggctggcc accaccggcg
3481 taccctgcgc cgctgtgttg gatgttgtga ccgaagccgg ccgggactgg ctgctgctgg
3541 gcgaggtccc tggccaggat ctgctgagca gccaccttgc ccccgctgag aaggtttcta
3601 tcatggccga tgcaatgcgg cgcctgcaca ccctggaccc cgctacctgc cccttcgacc
3661 accaggctaa gcatcggatc gagcgtgctc ggacccgcat ggaggccggc ctggtggacc
3721 aggacgacct ggacgaggag catcagggcc tggcccccgc tgaactgttc gcccgactga
3781 aagcccgcat gccggacggt gaggacctgg ttgtcacaca cggagatgcc tgcctcccta
3841 acatcatggt cgagaatggc cgcttctccg gcttcatcga ctgcggtcgc ctaggagttg
3901 ccgaccgcta ccaggacatc gccctggcca cccgcgacat cgctgaggag cttggcggcg
3961 agtgggccga ccgcttctta gtcttgtacg gcatcgcagc tcccgacagc cagcgcatcg
4021 ccttctaccg cttgctcgac gagttctttt aatgatctag aaccggtcat ggccgcaata
4081 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgttcga actagatgct
4141 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
4201 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
4261 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
4321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
4381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
4441 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
4501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
4561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
4621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
4681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
4741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
4801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
4861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
4921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
4981 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
5041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
5101 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
5161 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa
5221 tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct
5281 gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg
5341 agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc
5401 ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta
5461 ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc
5521 cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt
5581 cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac
5641 ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt
5701 tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc
5761 catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt
5821 gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata
5881 gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga
5941 tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag
6001 catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa
6061 agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt
6121 attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt aatattaagg
6181 tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
6241 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
6301 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctct
//
